Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection
Resistance of Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151 S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory c...
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| Veröffentlicht in: | Veterinary microbiology 2005-03, Vol.106 (1), p.97-102 |
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| creator | Haveri, M. Suominen, S. Rantala, L. Honkanen-Buzalski, T. Pyörälä, S. |
| description | Resistance of
Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151
S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory concentration (MIC) by a standard agar dilution technique and direct testing of β-lactamase production using a chromogenic cephalosporin, nitrocefin. The results from these tests were compared with the presence of the β-lactamase (
blaZ) gene in the isolates, which was detected by polymerase chain reaction (PCR). Testing β-lactamase production with nitrocefin was more predictive for the presence of the
blaZ gene than the agar dilution method and the results of the former agreed highly with the presence of the
blaZ gene in the isolates. In contrast, the resistance breakpoint generally used in the agar dilution method may be too high for prediction of penicillin resistance in
S. aureus isolates with borderline MICs. Using this method, 40% of the isolates possessing the
blaZ gene were classified as susceptible; however, majority of these isolates produced β-lactamase when tested with nitrocefin. |
| doi_str_mv | 10.1016/j.vetmic.2004.12.015 |
| format | Article |
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Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151
S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory concentration (MIC) by a standard agar dilution technique and direct testing of β-lactamase production using a chromogenic cephalosporin, nitrocefin. The results from these tests were compared with the presence of the β-lactamase (
blaZ) gene in the isolates, which was detected by polymerase chain reaction (PCR). Testing β-lactamase production with nitrocefin was more predictive for the presence of the
blaZ gene than the agar dilution method and the results of the former agreed highly with the presence of the
blaZ gene in the isolates. In contrast, the resistance breakpoint generally used in the agar dilution method may be too high for prediction of penicillin resistance in
S. aureus isolates with borderline MICs. Using this method, 40% of the isolates possessing the
blaZ gene were classified as susceptible; however, majority of these isolates produced β-lactamase when tested with nitrocefin.</description><identifier>ISSN: 0378-1135</identifier><identifier>EISSN: 1873-2542</identifier><identifier>DOI: 10.1016/j.vetmic.2004.12.015</identifier><identifier>PMID: 15737478</identifier><identifier>CODEN: VMICDQ</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Anti-Bacterial Agents - pharmacology ; Bacteriology ; beta-Lactamases - genetics ; beta-Lactamases - metabolism ; Biological and medical sciences ; Cattle ; Cephalosporins - metabolism ; DNA, Bacterial - chemistry ; DNA, Bacterial - genetics ; Female ; Fundamental and applied biological sciences. Psychology ; Mastitis ; Mastitis, Bovine - drug therapy ; Mastitis, Bovine - microbiology ; Microbial Sensitivity Tests - veterinary ; Microbiology ; Miscellaneous ; Penicillin G - pharmacology ; Penicillin resistance ; Penicillin Resistance - genetics ; Polymerase Chain Reaction - veterinary ; S. aureus ; Staphylococcal Infections - drug therapy ; Staphylococcal Infections - microbiology ; Staphylococcal Infections - veterinary ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - enzymology ; Staphylococcus aureus - genetics ; Staphylococcus aureus - isolation & purification ; Statistics, Nonparametric ; β-Lactamase</subject><ispartof>Veterinary microbiology, 2005-03, Vol.106 (1), p.97-102</ispartof><rights>2005 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c421t-7b46e7f17ba32b8fcf09d45c1b983d8922e69d7dfbe9d290cedbc4b38f3a8133</citedby><cites>FETCH-LOGICAL-c421t-7b46e7f17ba32b8fcf09d45c1b983d8922e69d7dfbe9d290cedbc4b38f3a8133</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.vetmic.2004.12.015$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16582165$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15737478$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haveri, M.</creatorcontrib><creatorcontrib>Suominen, S.</creatorcontrib><creatorcontrib>Rantala, L.</creatorcontrib><creatorcontrib>Honkanen-Buzalski, T.</creatorcontrib><creatorcontrib>Pyörälä, S.</creatorcontrib><title>Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection</title><title>Veterinary microbiology</title><addtitle>Vet Microbiol</addtitle><description>Resistance of
Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151
S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory concentration (MIC) by a standard agar dilution technique and direct testing of β-lactamase production using a chromogenic cephalosporin, nitrocefin. The results from these tests were compared with the presence of the β-lactamase (
blaZ) gene in the isolates, which was detected by polymerase chain reaction (PCR). Testing β-lactamase production with nitrocefin was more predictive for the presence of the
blaZ gene than the agar dilution method and the results of the former agreed highly with the presence of the
blaZ gene in the isolates. In contrast, the resistance breakpoint generally used in the agar dilution method may be too high for prediction of penicillin resistance in
S. aureus isolates with borderline MICs. Using this method, 40% of the isolates possessing the
blaZ gene were classified as susceptible; however, majority of these isolates produced β-lactamase when tested with nitrocefin.</description><subject>Animals</subject><subject>Anti-Bacterial Agents - pharmacology</subject><subject>Bacteriology</subject><subject>beta-Lactamases - genetics</subject><subject>beta-Lactamases - metabolism</subject><subject>Biological and medical sciences</subject><subject>Cattle</subject><subject>Cephalosporins - metabolism</subject><subject>DNA, Bacterial - chemistry</subject><subject>DNA, Bacterial - genetics</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mastitis</subject><subject>Mastitis, Bovine - drug therapy</subject><subject>Mastitis, Bovine - microbiology</subject><subject>Microbial Sensitivity Tests - veterinary</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Penicillin G - pharmacology</subject><subject>Penicillin resistance</subject><subject>Penicillin Resistance - genetics</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>S. aureus</subject><subject>Staphylococcal Infections - drug therapy</subject><subject>Staphylococcal Infections - microbiology</subject><subject>Staphylococcal Infections - veterinary</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - enzymology</subject><subject>Staphylococcus aureus - genetics</subject><subject>Staphylococcus aureus - isolation & purification</subject><subject>Statistics, Nonparametric</subject><subject>β-Lactamase</subject><issn>0378-1135</issn><issn>1873-2542</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1q3TAQhUVoaW7SvkEp2rQ7u_rxnzaFcmmTQiCLZi9kaZToYluuJF-4b5FHji42za7dzDDMNwfpHIQ-UlJSQpuvh_IIaXS6ZIRUJWUlofUF2tGu5QWrK_YG7Qhvu4JSXl-iqxgPJIOiIe_QJa1b3lZtt0PPez_OKrjoJ-wtnp9g8uk0O43VZPDj38lAAp3cRsHktBsGN-EbHCC6mNSk4bz6ndT8dBq89lovEaslQG5ZflAJDLbBj7j3RzcBdlMKalTjqMIpD3bVf4_eWjVE-LD1a_Tw88fD_ra4u7_5tf9-V-iK0VS0fdVAa2nbK876zmpLhKlqTXvRcdMJxqARpjW2B2GYIBpMr6ued5arjnJ-jb6ssnPwfxaISY4uahgGNYFfomyyPY0QzX9BKrJ63YgMViuog48xgJVzcOe_SUrkOTF5kGti8pyYpEzmxPLZp01_6Ucwr0dbRBn4vAEqajXYkK128ZVr6o7lkrlvKwfZtaODIKN2kGMxLmRrpfHu3y95AU73uxA</recordid><startdate>20050320</startdate><enddate>20050320</enddate><creator>Haveri, M.</creator><creator>Suominen, S.</creator><creator>Rantala, L.</creator><creator>Honkanen-Buzalski, T.</creator><creator>Pyörälä, S.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20050320</creationdate><title>Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection</title><author>Haveri, M. ; Suominen, S. ; Rantala, L. ; Honkanen-Buzalski, T. ; Pyörälä, S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c421t-7b46e7f17ba32b8fcf09d45c1b983d8922e69d7dfbe9d290cedbc4b38f3a8133</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Anti-Bacterial Agents - pharmacology</topic><topic>Bacteriology</topic><topic>beta-Lactamases - genetics</topic><topic>beta-Lactamases - metabolism</topic><topic>Biological and medical sciences</topic><topic>Cattle</topic><topic>Cephalosporins - metabolism</topic><topic>DNA, Bacterial - chemistry</topic><topic>DNA, Bacterial - genetics</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mastitis</topic><topic>Mastitis, Bovine - drug therapy</topic><topic>Mastitis, Bovine - microbiology</topic><topic>Microbial Sensitivity Tests - veterinary</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Penicillin G - pharmacology</topic><topic>Penicillin resistance</topic><topic>Penicillin Resistance - genetics</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>S. aureus</topic><topic>Staphylococcal Infections - drug therapy</topic><topic>Staphylococcal Infections - microbiology</topic><topic>Staphylococcal Infections - veterinary</topic><topic>Staphylococcus aureus</topic><topic>Staphylococcus aureus - drug effects</topic><topic>Staphylococcus aureus - enzymology</topic><topic>Staphylococcus aureus - genetics</topic><topic>Staphylococcus aureus - isolation & purification</topic><topic>Statistics, Nonparametric</topic><topic>β-Lactamase</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haveri, M.</creatorcontrib><creatorcontrib>Suominen, S.</creatorcontrib><creatorcontrib>Rantala, L.</creatorcontrib><creatorcontrib>Honkanen-Buzalski, T.</creatorcontrib><creatorcontrib>Pyörälä, S.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Veterinary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haveri, M.</au><au>Suominen, S.</au><au>Rantala, L.</au><au>Honkanen-Buzalski, T.</au><au>Pyörälä, S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection</atitle><jtitle>Veterinary microbiology</jtitle><addtitle>Vet Microbiol</addtitle><date>2005-03-20</date><risdate>2005</risdate><volume>106</volume><issue>1</issue><spage>97</spage><epage>102</epage><pages>97-102</pages><issn>0378-1135</issn><eissn>1873-2542</eissn><coden>VMICDQ</coden><abstract>Resistance of
Staphylococcus aureus to penicillin G is common among isolates from bovine mastitis. We determined phenotypic resistance to penicillin G for 151
S. aureus isolates derived from dairy cows with intramammary infection by two methods. The methods were determination of minimum inhibitory concentration (MIC) by a standard agar dilution technique and direct testing of β-lactamase production using a chromogenic cephalosporin, nitrocefin. The results from these tests were compared with the presence of the β-lactamase (
blaZ) gene in the isolates, which was detected by polymerase chain reaction (PCR). Testing β-lactamase production with nitrocefin was more predictive for the presence of the
blaZ gene than the agar dilution method and the results of the former agreed highly with the presence of the
blaZ gene in the isolates. In contrast, the resistance breakpoint generally used in the agar dilution method may be too high for prediction of penicillin resistance in
S. aureus isolates with borderline MICs. Using this method, 40% of the isolates possessing the
blaZ gene were classified as susceptible; however, majority of these isolates produced β-lactamase when tested with nitrocefin.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15737478</pmid><doi>10.1016/j.vetmic.2004.12.015</doi><tpages>6</tpages></addata></record> |
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| ispartof | Veterinary microbiology, 2005-03, Vol.106 (1), p.97-102 |
| issn | 0378-1135 1873-2542 |
| language | eng |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | Animals Anti-Bacterial Agents - pharmacology Bacteriology beta-Lactamases - genetics beta-Lactamases - metabolism Biological and medical sciences Cattle Cephalosporins - metabolism DNA, Bacterial - chemistry DNA, Bacterial - genetics Female Fundamental and applied biological sciences. Psychology Mastitis Mastitis, Bovine - drug therapy Mastitis, Bovine - microbiology Microbial Sensitivity Tests - veterinary Microbiology Miscellaneous Penicillin G - pharmacology Penicillin resistance Penicillin Resistance - genetics Polymerase Chain Reaction - veterinary S. aureus Staphylococcal Infections - drug therapy Staphylococcal Infections - microbiology Staphylococcal Infections - veterinary Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - enzymology Staphylococcus aureus - genetics Staphylococcus aureus - isolation & purification Statistics, Nonparametric β-Lactamase |
| title | Comparison of phenotypic and genotypic detection of penicillin G resistance of Staphylococcus aureus isolated from bovine intramammary infection |
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