Fabrication of dissolved O2 metric uric acid biosensor using uricase epoxy resin biocomposite membrane

Uricase purified from 20-day-old leaves of cowpea was immobilized on to epoxy resin membrane with 80% retention of initial activity of free enzyme and a conjugation yield of 0.056 mg/cm(2). The uricase epoxy resin bioconjugate membrane was mounted over the sensing part of the combined electrode of &...

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Veröffentlicht in:	Analytica chimica acta 2009-08, Vol.647 (2), p.195-201
Hauptverfasser:	Arora, Jyoti, Nandwani, Seep, Bhambi, Manu, Pundir, C S
Format:	Artikel
Sprache:	eng
Schlagworte:	Biosensing Techniques - instrumentation Biosensing Techniques - methods Enzymes, Immobilized - chemistry Enzymes, Immobilized - isolation & purification Enzymes, Immobilized - metabolism Epoxy Resins - chemistry Equipment Design Fabaceae - chemistry Female Humans Kinetics Male Membranes, Artificial Microscopy, Electron, Scanning Oxidation-Reduction Oxygen - analysis Plant Leaves - chemistry Sensitivity and Specificity Urate Oxidase - chemistry Urate Oxidase - isolation & purification Urate Oxidase - metabolism Uric Acid - blood
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