Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: A window on renal citrate metabolism
Background: An increase in urinary citrate excretion is associated with a decrease in activity of renal cortical cytosolic ATP citrate lyase (ACL) and mitochondrial aconitase (m‐aconitase). Because potassium‐magnesium citrate causes an increase in urinary citrate excretion, we decided to assess its...
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| Veröffentlicht in: | International journal of urology 2005-02, Vol.12 (2), p.140-144 |
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| creator | TOSUKHOWONG, PIYARATANA TUNGSANGA, KRIANG PHONGUDOM, SUMET SRIBOONLUE, POTE |
| description | Background: An increase in urinary citrate excretion is associated with a decrease in activity of renal cortical cytosolic ATP citrate lyase (ACL) and mitochondrial aconitase (m‐aconitase). Because potassium‐magnesium citrate causes an increase in urinary citrate excretion, we decided to assess its effects on ACL and m‐aconitase in the leukocytes of renal stone patients.
Methods: Twenty male renal stone patients were supplemented with potassium‐magnesium citrate twice daily (i.e. 42 mEq potassium, 21 mEq magnesium, and 63 mEq citrate per day) for a period of 1 month. Two 24‐h urine and one 15‐mL heparinized blood samples were collected from each patient before and after supplementation. Urine samples were analyzed for relevant biochemical compositions. Leukocytes were separated from blood samples by centrifugation and assayed for ACL and m‐aconitase activity.
Results: Supplementation with potassium‐magnesium citrate significantly increased urinary pH (P |
| doi_str_mv | 10.1111/j.1442-2042.2005.01001.x |
| format | Article |
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Methods: Twenty male renal stone patients were supplemented with potassium‐magnesium citrate twice daily (i.e. 42 mEq potassium, 21 mEq magnesium, and 63 mEq citrate per day) for a period of 1 month. Two 24‐h urine and one 15‐mL heparinized blood samples were collected from each patient before and after supplementation. Urine samples were analyzed for relevant biochemical compositions. Leukocytes were separated from blood samples by centrifugation and assayed for ACL and m‐aconitase activity.
Results: Supplementation with potassium‐magnesium citrate significantly increased urinary pH (P < 0.005) and excretions of potassium (P < 0.001), magnesium (P < 0.001) and citrate (P < 0.0001). The activity of both ACL and m‐aconitase were significantly decreased (P < 0.004 and P < 0.02 respectively). The decrease in ACL activity was inversely correlated with an increase in urinary excretion of both potassium (r = −0.620, P < 0.0001) and citrate (r = −0.451, P < 0.004). A similar inverse correlation was observed between m‐aconitase activity and urinary excretion of citrate (r = −0.322, P < 0.043).
Conclusion: Changes in enzyme activity, related to citrate metabolism in leukocytes, might reflect the status of renal tubular cells.]]></description><identifier>ISSN: 0919-8172</identifier><identifier>EISSN: 1442-2042</identifier><identifier>DOI: 10.1111/j.1442-2042.2005.01001.x</identifier><identifier>PMID: 15733107</identifier><language>eng</language><publisher>Melbourne, Australia: Blackwell Science Pty</publisher><subject>Aconitate Hydratase - drug effects ; Adult ; ATP Citrate (pro-S)-Lyase - drug effects ; Citric Acid - urine ; Diuretics - pharmacology ; Humans ; Hydrogen-Ion Concentration ; Kidney Calculi - enzymology ; leukocyte ATP citrate lyase ; leukocyte mitochondrial aconitase ; Leukocytes - enzymology ; Magnesium - urine ; Magnesium Compounds - pharmacology ; Male ; Middle Aged ; Potassium - urine ; Potassium Citrate - pharmacology ; renal stone ; urinary citrate ; Urine - chemistry</subject><ispartof>International journal of urology, 2005-02, Vol.12 (2), p.140-144</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4581-58507a793062a5814d19900ce1667c4a8168a96da6dd3eef0ea035447b7641fa3</citedby><cites>FETCH-LOGICAL-c4581-58507a793062a5814d19900ce1667c4a8168a96da6dd3eef0ea035447b7641fa3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1442-2042.2005.01001.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1442-2042.2005.01001.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27923,27924,45573,45574</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15733107$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>TOSUKHOWONG, PIYARATANA</creatorcontrib><creatorcontrib>TUNGSANGA, KRIANG</creatorcontrib><creatorcontrib>PHONGUDOM, SUMET</creatorcontrib><creatorcontrib>SRIBOONLUE, POTE</creatorcontrib><title>Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: A window on renal citrate metabolism</title><title>International journal of urology</title><addtitle>Int J Urol</addtitle><description><![CDATA[Background: An increase in urinary citrate excretion is associated with a decrease in activity of renal cortical cytosolic ATP citrate lyase (ACL) and mitochondrial aconitase (m‐aconitase). Because potassium‐magnesium citrate causes an increase in urinary citrate excretion, we decided to assess its effects on ACL and m‐aconitase in the leukocytes of renal stone patients.
Methods: Twenty male renal stone patients were supplemented with potassium‐magnesium citrate twice daily (i.e. 42 mEq potassium, 21 mEq magnesium, and 63 mEq citrate per day) for a period of 1 month. Two 24‐h urine and one 15‐mL heparinized blood samples were collected from each patient before and after supplementation. Urine samples were analyzed for relevant biochemical compositions. Leukocytes were separated from blood samples by centrifugation and assayed for ACL and m‐aconitase activity.
Results: Supplementation with potassium‐magnesium citrate significantly increased urinary pH (P < 0.005) and excretions of potassium (P < 0.001), magnesium (P < 0.001) and citrate (P < 0.0001). The activity of both ACL and m‐aconitase were significantly decreased (P < 0.004 and P < 0.02 respectively). The decrease in ACL activity was inversely correlated with an increase in urinary excretion of both potassium (r = −0.620, P < 0.0001) and citrate (r = −0.451, P < 0.004). A similar inverse correlation was observed between m‐aconitase activity and urinary excretion of citrate (r = −0.322, P < 0.043).
Conclusion: Changes in enzyme activity, related to citrate metabolism in leukocytes, might reflect the status of renal tubular cells.]]></description><subject>Aconitate Hydratase - drug effects</subject><subject>Adult</subject><subject>ATP Citrate (pro-S)-Lyase - drug effects</subject><subject>Citric Acid - urine</subject><subject>Diuretics - pharmacology</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Kidney Calculi - enzymology</subject><subject>leukocyte ATP citrate lyase</subject><subject>leukocyte mitochondrial aconitase</subject><subject>Leukocytes - enzymology</subject><subject>Magnesium - urine</subject><subject>Magnesium Compounds - pharmacology</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Potassium - urine</subject><subject>Potassium Citrate - pharmacology</subject><subject>renal stone</subject><subject>urinary citrate</subject><subject>Urine - chemistry</subject><issn>0919-8172</issn><issn>1442-2042</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFu1DAQhi0EokvhFZBP3BLs2I4TJA5LVUpRKQi1gpvldSbgbWJvY4fdvBTPiNNdliuWJY88__fPSD9CmJKcpvN6nVPOi6wgvMgLQkROKCE03z1Ci2PjMVqQmtZZRWVxgp6FsE4SVtDqKTqhQjJGiVyg3-dtCyYG7Fu88VGHYMc-6_UPB3OFjY2DjoDDuNl00IOLOlrvcLpmij74zhq8vPlyFHaTDoC1a3Bvozc_vWsGqzusjXc2PvRMtL9snLB1uIPxzicjCG_wEm-ta_x29h7AJeavZw9Rr9Kk0D9HT1rdBXhxeE_R7fvzm7MP2dXni8uz5VVmuKhoJipBpJY1I2Wh0wdvaF0TYoCWpTRcV7SsdF02umwaBtAS0IQJzuVKlpy2mp2iV3vfzeDvRwhR9TYY6DrtwI9BlZKXXHCRhNVeaAYfwgCt2gy218OkKFFzVmqt5kjUHImas1IPWaldQl8eZoyrHpp_4CGcJHi7F2xtB9N_G6vLj7dzlfhsz9sQYXfk9XCX9mdSqG_XF-pafP307jsjirM_gvm1bA</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>TOSUKHOWONG, PIYARATANA</creator><creator>TUNGSANGA, KRIANG</creator><creator>PHONGUDOM, SUMET</creator><creator>SRIBOONLUE, POTE</creator><general>Blackwell Science Pty</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: A window on renal citrate metabolism</title><author>TOSUKHOWONG, PIYARATANA ; TUNGSANGA, KRIANG ; PHONGUDOM, SUMET ; SRIBOONLUE, POTE</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4581-58507a793062a5814d19900ce1667c4a8168a96da6dd3eef0ea035447b7641fa3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aconitate Hydratase - drug effects</topic><topic>Adult</topic><topic>ATP Citrate (pro-S)-Lyase - drug effects</topic><topic>Citric Acid - urine</topic><topic>Diuretics - pharmacology</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Kidney Calculi - enzymology</topic><topic>leukocyte ATP citrate lyase</topic><topic>leukocyte mitochondrial aconitase</topic><topic>Leukocytes - enzymology</topic><topic>Magnesium - urine</topic><topic>Magnesium Compounds - pharmacology</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Potassium - urine</topic><topic>Potassium Citrate - pharmacology</topic><topic>renal stone</topic><topic>urinary citrate</topic><topic>Urine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>TOSUKHOWONG, PIYARATANA</creatorcontrib><creatorcontrib>TUNGSANGA, KRIANG</creatorcontrib><creatorcontrib>PHONGUDOM, SUMET</creatorcontrib><creatorcontrib>SRIBOONLUE, POTE</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of urology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>TOSUKHOWONG, PIYARATANA</au><au>TUNGSANGA, KRIANG</au><au>PHONGUDOM, SUMET</au><au>SRIBOONLUE, POTE</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: A window on renal citrate metabolism</atitle><jtitle>International journal of urology</jtitle><addtitle>Int J Urol</addtitle><date>2005-02</date><risdate>2005</risdate><volume>12</volume><issue>2</issue><spage>140</spage><epage>144</epage><pages>140-144</pages><issn>0919-8172</issn><eissn>1442-2042</eissn><abstract><![CDATA[Background: An increase in urinary citrate excretion is associated with a decrease in activity of renal cortical cytosolic ATP citrate lyase (ACL) and mitochondrial aconitase (m‐aconitase). Because potassium‐magnesium citrate causes an increase in urinary citrate excretion, we decided to assess its effects on ACL and m‐aconitase in the leukocytes of renal stone patients.
Methods: Twenty male renal stone patients were supplemented with potassium‐magnesium citrate twice daily (i.e. 42 mEq potassium, 21 mEq magnesium, and 63 mEq citrate per day) for a period of 1 month. Two 24‐h urine and one 15‐mL heparinized blood samples were collected from each patient before and after supplementation. Urine samples were analyzed for relevant biochemical compositions. Leukocytes were separated from blood samples by centrifugation and assayed for ACL and m‐aconitase activity.
Results: Supplementation with potassium‐magnesium citrate significantly increased urinary pH (P < 0.005) and excretions of potassium (P < 0.001), magnesium (P < 0.001) and citrate (P < 0.0001). The activity of both ACL and m‐aconitase were significantly decreased (P < 0.004 and P < 0.02 respectively). The decrease in ACL activity was inversely correlated with an increase in urinary excretion of both potassium (r = −0.620, P < 0.0001) and citrate (r = −0.451, P < 0.004). A similar inverse correlation was observed between m‐aconitase activity and urinary excretion of citrate (r = −0.322, P < 0.043).
Conclusion: Changes in enzyme activity, related to citrate metabolism in leukocytes, might reflect the status of renal tubular cells.]]></abstract><cop>Melbourne, Australia</cop><pub>Blackwell Science Pty</pub><pmid>15733107</pmid><doi>10.1111/j.1442-2042.2005.01001.x</doi><tpages>5</tpages></addata></record> |
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| subjects | Aconitate Hydratase - drug effects Adult ATP Citrate (pro-S)-Lyase - drug effects Citric Acid - urine Diuretics - pharmacology Humans Hydrogen-Ion Concentration Kidney Calculi - enzymology leukocyte ATP citrate lyase leukocyte mitochondrial aconitase Leukocytes - enzymology Magnesium - urine Magnesium Compounds - pharmacology Male Middle Aged Potassium - urine Potassium Citrate - pharmacology renal stone urinary citrate Urine - chemistry |
| title | Effects of potassium-magnesium citrate supplementation on cytosolic ATP citrate lyase and mitochondrial aconitase activity in leukocytes: A window on renal citrate metabolism |
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