Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells
Glial‐derived nexin (GDN) is a proteinase inhibitor secreted from glial cells and it can enhance neuronal function. However, its expression and function in neuronal differentiation are not, as yet, well‐known. In the present study, we analyzed glial‐derived nexin gene expression in dissociated neura...
Gespeichert in:
Veröffentlicht in: | International journal of developmental neuroscience 2005-02, Vol.23 (1), p.9-14 |
---|---|
Hauptverfasser: | , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
container_end_page | 14 |
---|---|
container_issue | 1 |
container_start_page | 9 |
container_title | International journal of developmental neuroscience |
container_volume | 23 |
creator | Lin, Hsingchi J. Shaffer, Kara M. Sun, Zairen Jay, Gilbert He, Wei‐wu Ma, Wu |
description | Glial‐derived nexin (GDN) is a proteinase inhibitor secreted from glial cells and it can enhance neuronal function. However, its expression and function in neuronal differentiation are not, as yet, well‐known. In the present study, we analyzed glial‐derived nexin gene expression in dissociated neural stem/progenitor cells (NS/PCs) (D0) from the embryonic mouse cerebral cortex, expanded NS/PC cultures (D4 and D10 cultures) and cultured neurons (E15) using a semi‐quantitative RT‐PCR assay. Our data suggest that mouse GDN, homologue of human GDN, was significantly up‐regulated in the expanded NS/PC cultures and cultured neurons. To analyze its function in neuronal differentiation, human GDN cDNA was cloned into bicistronic plasmids containing green fluorescent protein (GFP) and the resulting plasmids were transfected into rodent primary NS/PCs and non‐neuronal human embryonic kidney (HEK) cells. Our data suggest that the ectopic expression of human GDN triggered the expression of the neuronal marker TuJ1 in both NS/PCs and HEK cells. We conclude that GDN is up‐regulated during neuronal differentiation and plays a role in transforming non‐neuronal HEK cells into neuron‐like cells. |
doi_str_mv | 10.1016/j.ijdevneu.2004.09.007 |
format | Article |
fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67460705</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>67460705</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3757-a04fe0ab5ab04a2ebcc34d87ac9a88664248094b9b622bb713dfa1b2e8ac27fd3</originalsourceid><addsrcrecordid>eNqNkUFP3DAQhS1EBQvtX0A59UTSSeLEzrGCBXaL6KVUvVl2PEHeep2tnQB748K9v7G_pI52q_bY00gz33vP8iPkLIcsh7z-sMrMSuOjwzErAGgGTQbADsgs56xMKaPfDskMWFmnFaP8mJyEsAKAqgJ6RI7zipXAeTEjr9fWSPvr5adGbx5RJw6fjTtPZKJN16FHN8S73Sb4vPEYQiQe0GGiR2_cQ6RH3ztp_6UH00eDwUsXut6vQ3Iz_5S0aG1IjBv6vSZGWvMdd4e35E0nbcB3-3lK7q_mXy5u0tvP14uLj7dpW7KKpRJohyBVJRVQWaBq25JqzmTbSM7rmhaUQ0NVo-qiUIrlpe5krgrksi1Yp8tT8n7nu_H9jxHDINYmTC-QDvsxiJrRGhhUEax3YOv7EDx2YuPNWvqtyEFMBYiV-FOAmAoQ0IhYQBSe7RNGtUb9V7b_8QgsdsCTsbj9T1uxvLxbLpaX86938_tpD80U9hsJoKD1</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>67460705</pqid></control><display><type>article</type><title>Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells</title><source>MEDLINE</source><source>Wiley Online Library Journals Frontfile Complete</source><source>Elsevier ScienceDirect Journals</source><creator>Lin, Hsingchi J. ; Shaffer, Kara M. ; Sun, Zairen ; Jay, Gilbert ; He, Wei‐wu ; Ma, Wu</creator><creatorcontrib>Lin, Hsingchi J. ; Shaffer, Kara M. ; Sun, Zairen ; Jay, Gilbert ; He, Wei‐wu ; Ma, Wu</creatorcontrib><description>Glial‐derived nexin (GDN) is a proteinase inhibitor secreted from glial cells and it can enhance neuronal function. However, its expression and function in neuronal differentiation are not, as yet, well‐known. In the present study, we analyzed glial‐derived nexin gene expression in dissociated neural stem/progenitor cells (NS/PCs) (D0) from the embryonic mouse cerebral cortex, expanded NS/PC cultures (D4 and D10 cultures) and cultured neurons (E15) using a semi‐quantitative RT‐PCR assay. Our data suggest that mouse GDN, homologue of human GDN, was significantly up‐regulated in the expanded NS/PC cultures and cultured neurons. To analyze its function in neuronal differentiation, human GDN cDNA was cloned into bicistronic plasmids containing green fluorescent protein (GFP) and the resulting plasmids were transfected into rodent primary NS/PCs and non‐neuronal human embryonic kidney (HEK) cells. Our data suggest that the ectopic expression of human GDN triggered the expression of the neuronal marker TuJ1 in both NS/PCs and HEK cells. We conclude that GDN is up‐regulated during neuronal differentiation and plays a role in transforming non‐neuronal HEK cells into neuron‐like cells.</description><identifier>ISSN: 0736-5748</identifier><identifier>EISSN: 1873-474X</identifier><identifier>DOI: 10.1016/j.ijdevneu.2004.09.007</identifier><identifier>PMID: 15730882</identifier><language>eng</language><publisher>United States</publisher><subject>Amyloid beta-Protein Precursor - physiology ; Animals ; Blotting, Northern - methods ; Bromodeoxyuridine - metabolism ; Cell Differentiation - physiology ; Cells, Cultured ; Cloning, Molecular - methods ; Differential display ; Embryo, Mammalian ; Gene Expression - physiology ; Glial‐derived nexin ; Green Fluorescent Proteins - metabolism ; HEK ; Humans ; Immunohistochemistry - methods ; Indoles ; Mice ; Neural stem cells ; Neurogenesis ; Neurons - cytology ; Neurons - physiology ; Protease Nexins ; Receptors, Cell Surface - physiology ; Reverse Transcriptase Polymerase Chain Reaction - methods ; RNA, Messenger - biosynthesis ; Stem Cells - physiology ; Transfection ; Transfection - methods ; Tubulin - metabolism</subject><ispartof>International journal of developmental neuroscience, 2005-02, Vol.23 (1), p.9-14</ispartof><rights>2005 ISDN</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3757-a04fe0ab5ab04a2ebcc34d87ac9a88664248094b9b622bb713dfa1b2e8ac27fd3</citedby><cites>FETCH-LOGICAL-c3757-a04fe0ab5ab04a2ebcc34d87ac9a88664248094b9b622bb713dfa1b2e8ac27fd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.ijdevneu.2004.09.007$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.ijdevneu.2004.09.007$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15730882$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lin, Hsingchi J.</creatorcontrib><creatorcontrib>Shaffer, Kara M.</creatorcontrib><creatorcontrib>Sun, Zairen</creatorcontrib><creatorcontrib>Jay, Gilbert</creatorcontrib><creatorcontrib>He, Wei‐wu</creatorcontrib><creatorcontrib>Ma, Wu</creatorcontrib><title>Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells</title><title>International journal of developmental neuroscience</title><addtitle>Int J Dev Neurosci</addtitle><description>Glial‐derived nexin (GDN) is a proteinase inhibitor secreted from glial cells and it can enhance neuronal function. However, its expression and function in neuronal differentiation are not, as yet, well‐known. In the present study, we analyzed glial‐derived nexin gene expression in dissociated neural stem/progenitor cells (NS/PCs) (D0) from the embryonic mouse cerebral cortex, expanded NS/PC cultures (D4 and D10 cultures) and cultured neurons (E15) using a semi‐quantitative RT‐PCR assay. Our data suggest that mouse GDN, homologue of human GDN, was significantly up‐regulated in the expanded NS/PC cultures and cultured neurons. To analyze its function in neuronal differentiation, human GDN cDNA was cloned into bicistronic plasmids containing green fluorescent protein (GFP) and the resulting plasmids were transfected into rodent primary NS/PCs and non‐neuronal human embryonic kidney (HEK) cells. Our data suggest that the ectopic expression of human GDN triggered the expression of the neuronal marker TuJ1 in both NS/PCs and HEK cells. We conclude that GDN is up‐regulated during neuronal differentiation and plays a role in transforming non‐neuronal HEK cells into neuron‐like cells.</description><subject>Amyloid beta-Protein Precursor - physiology</subject><subject>Animals</subject><subject>Blotting, Northern - methods</subject><subject>Bromodeoxyuridine - metabolism</subject><subject>Cell Differentiation - physiology</subject><subject>Cells, Cultured</subject><subject>Cloning, Molecular - methods</subject><subject>Differential display</subject><subject>Embryo, Mammalian</subject><subject>Gene Expression - physiology</subject><subject>Glial‐derived nexin</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>HEK</subject><subject>Humans</subject><subject>Immunohistochemistry - methods</subject><subject>Indoles</subject><subject>Mice</subject><subject>Neural stem cells</subject><subject>Neurogenesis</subject><subject>Neurons - cytology</subject><subject>Neurons - physiology</subject><subject>Protease Nexins</subject><subject>Receptors, Cell Surface - physiology</subject><subject>Reverse Transcriptase Polymerase Chain Reaction - methods</subject><subject>RNA, Messenger - biosynthesis</subject><subject>Stem Cells - physiology</subject><subject>Transfection</subject><subject>Transfection - methods</subject><subject>Tubulin - metabolism</subject><issn>0736-5748</issn><issn>1873-474X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkUFP3DAQhS1EBQvtX0A59UTSSeLEzrGCBXaL6KVUvVl2PEHeep2tnQB748K9v7G_pI52q_bY00gz33vP8iPkLIcsh7z-sMrMSuOjwzErAGgGTQbADsgs56xMKaPfDskMWFmnFaP8mJyEsAKAqgJ6RI7zipXAeTEjr9fWSPvr5adGbx5RJw6fjTtPZKJN16FHN8S73Sb4vPEYQiQe0GGiR2_cQ6RH3ztp_6UH00eDwUsXut6vQ3Iz_5S0aG1IjBv6vSZGWvMdd4e35E0nbcB3-3lK7q_mXy5u0tvP14uLj7dpW7KKpRJohyBVJRVQWaBq25JqzmTbSM7rmhaUQ0NVo-qiUIrlpe5krgrksi1Yp8tT8n7nu_H9jxHDINYmTC-QDvsxiJrRGhhUEax3YOv7EDx2YuPNWvqtyEFMBYiV-FOAmAoQ0IhYQBSe7RNGtUb9V7b_8QgsdsCTsbj9T1uxvLxbLpaX86938_tpD80U9hsJoKD1</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Lin, Hsingchi J.</creator><creator>Shaffer, Kara M.</creator><creator>Sun, Zairen</creator><creator>Jay, Gilbert</creator><creator>He, Wei‐wu</creator><creator>Ma, Wu</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells</title><author>Lin, Hsingchi J. ; Shaffer, Kara M. ; Sun, Zairen ; Jay, Gilbert ; He, Wei‐wu ; Ma, Wu</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3757-a04fe0ab5ab04a2ebcc34d87ac9a88664248094b9b622bb713dfa1b2e8ac27fd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Amyloid beta-Protein Precursor - physiology</topic><topic>Animals</topic><topic>Blotting, Northern - methods</topic><topic>Bromodeoxyuridine - metabolism</topic><topic>Cell Differentiation - physiology</topic><topic>Cells, Cultured</topic><topic>Cloning, Molecular - methods</topic><topic>Differential display</topic><topic>Embryo, Mammalian</topic><topic>Gene Expression - physiology</topic><topic>Glial‐derived nexin</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>HEK</topic><topic>Humans</topic><topic>Immunohistochemistry - methods</topic><topic>Indoles</topic><topic>Mice</topic><topic>Neural stem cells</topic><topic>Neurogenesis</topic><topic>Neurons - cytology</topic><topic>Neurons - physiology</topic><topic>Protease Nexins</topic><topic>Receptors, Cell Surface - physiology</topic><topic>Reverse Transcriptase Polymerase Chain Reaction - methods</topic><topic>RNA, Messenger - biosynthesis</topic><topic>Stem Cells - physiology</topic><topic>Transfection</topic><topic>Transfection - methods</topic><topic>Tubulin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lin, Hsingchi J.</creatorcontrib><creatorcontrib>Shaffer, Kara M.</creatorcontrib><creatorcontrib>Sun, Zairen</creatorcontrib><creatorcontrib>Jay, Gilbert</creatorcontrib><creatorcontrib>He, Wei‐wu</creatorcontrib><creatorcontrib>Ma, Wu</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of developmental neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lin, Hsingchi J.</au><au>Shaffer, Kara M.</au><au>Sun, Zairen</au><au>Jay, Gilbert</au><au>He, Wei‐wu</au><au>Ma, Wu</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells</atitle><jtitle>International journal of developmental neuroscience</jtitle><addtitle>Int J Dev Neurosci</addtitle><date>2005-02</date><risdate>2005</risdate><volume>23</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0736-5748</issn><eissn>1873-474X</eissn><abstract>Glial‐derived nexin (GDN) is a proteinase inhibitor secreted from glial cells and it can enhance neuronal function. However, its expression and function in neuronal differentiation are not, as yet, well‐known. In the present study, we analyzed glial‐derived nexin gene expression in dissociated neural stem/progenitor cells (NS/PCs) (D0) from the embryonic mouse cerebral cortex, expanded NS/PC cultures (D4 and D10 cultures) and cultured neurons (E15) using a semi‐quantitative RT‐PCR assay. Our data suggest that mouse GDN, homologue of human GDN, was significantly up‐regulated in the expanded NS/PC cultures and cultured neurons. To analyze its function in neuronal differentiation, human GDN cDNA was cloned into bicistronic plasmids containing green fluorescent protein (GFP) and the resulting plasmids were transfected into rodent primary NS/PCs and non‐neuronal human embryonic kidney (HEK) cells. Our data suggest that the ectopic expression of human GDN triggered the expression of the neuronal marker TuJ1 in both NS/PCs and HEK cells. We conclude that GDN is up‐regulated during neuronal differentiation and plays a role in transforming non‐neuronal HEK cells into neuron‐like cells.</abstract><cop>United States</cop><pmid>15730882</pmid><doi>10.1016/j.ijdevneu.2004.09.007</doi><tpages>6</tpages></addata></record> |
fulltext | fulltext |
identifier | ISSN: 0736-5748 |
ispartof | International journal of developmental neuroscience, 2005-02, Vol.23 (1), p.9-14 |
issn | 0736-5748 1873-474X |
language | eng |
recordid | cdi_proquest_miscellaneous_67460705 |
source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Elsevier ScienceDirect Journals |
subjects | Amyloid beta-Protein Precursor - physiology Animals Blotting, Northern - methods Bromodeoxyuridine - metabolism Cell Differentiation - physiology Cells, Cultured Cloning, Molecular - methods Differential display Embryo, Mammalian Gene Expression - physiology Glial‐derived nexin Green Fluorescent Proteins - metabolism HEK Humans Immunohistochemistry - methods Indoles Mice Neural stem cells Neurogenesis Neurons - cytology Neurons - physiology Protease Nexins Receptors, Cell Surface - physiology Reverse Transcriptase Polymerase Chain Reaction - methods RNA, Messenger - biosynthesis Stem Cells - physiology Transfection Transfection - methods Tubulin - metabolism |
title | Glial‐derived nexin, a differentially expressed gene during neuronal differentiation, transforms HEK cells into neuron‐like cells |
url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-03T21%3A15%3A48IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Glial%E2%80%90derived%20nexin,%20a%20differentially%20expressed%20gene%20during%20neuronal%20differentiation,%20transforms%20HEK%20cells%20into%20neuron%E2%80%90like%20cells&rft.jtitle=International%20journal%20of%20developmental%20neuroscience&rft.au=Lin,%20Hsingchi%20J.&rft.date=2005-02&rft.volume=23&rft.issue=1&rft.spage=9&rft.epage=14&rft.pages=9-14&rft.issn=0736-5748&rft.eissn=1873-474X&rft_id=info:doi/10.1016/j.ijdevneu.2004.09.007&rft_dat=%3Cproquest_cross%3E67460705%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=67460705&rft_id=info:pmid/15730882&rfr_iscdi=true |