Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial
Diagnostic differentiation between the ruminant pathogens Mycoplasma agalactiae and Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common e...
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| Veröffentlicht in: | The veterinary journal (1997) 2005-03, Vol.169 (2), p.268-275 |
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| creator | Bashiruddin, John B. Frey, Joachim Königsson, Malin Heldtander Johansson, Karl-Erik Hotzel, Helmut Diller, Roland de Santis, Paola Botelho, Ana Ayling, Roger D. Nicholas, Robin A.J. Thiaucourt, François Sachse, Konrad |
| description | Diagnostic differentiation between the ruminant pathogens
Mycoplasma agalactiae and
Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for
M. agalactiae and three systems for
M. bovis on a total of 41 strains of the two
Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three
M. bovis strains from small ruminants and two
M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes. |
| doi_str_mv | 10.1016/j.tvjl.2004.01.018 |
| format | Article |
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Mycoplasma agalactiae and
Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for
M. agalactiae and three systems for
M. bovis on a total of 41 strains of the two
Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three
M. bovis strains from small ruminants and two
M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.</description><identifier>ISSN: 1090-0233</identifier><identifier>EISSN: 1532-2971</identifier><identifier>DOI: 10.1016/j.tvjl.2004.01.018</identifier><identifier>PMID: 15727920</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>accuracy ; animal diseases ; Animals ; Cattle ; Cattle Diseases - epidemiology ; Cattle Diseases - microbiology ; diagnostic techniques ; disease diagnosis ; DNA Primers ; Europe - epidemiology ; Identification ; Mycoplasma agalactiae ; Mycoplasma agalactiae - classification ; Mycoplasma agalactiae - genetics ; Mycoplasma agalactiae - isolation & purification ; Mycoplasma bovis ; Mycoplasma bovis - classification ; Mycoplasma bovis - genetics ; Mycoplasma bovis - isolation & purification ; Mycoplasma Infections - epidemiology ; Mycoplasma Infections - microbiology ; Mycoplasma Infections - veterinary ; mycoplasmosis ; pathogen identification ; Polymerase chain reaction ; Polymerase Chain Reaction - standards ; Polymerase Chain Reaction - veterinary ; Predictive Value of Tests ; RNA, Ribosomal, 16S - genetics ; RNA, Ribosomal, 23S - genetics ; ruminants ; Sensitivity and Specificity ; Specificity ; strains ; test sensitivity ; test specificity</subject><ispartof>The veterinary journal (1997), 2005-03, Vol.169 (2), p.268-275</ispartof><rights>2004 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c378t-f44404e6fb172f3531b63c62ec7afd8a467b082a85cfa9b267abc5ea234a28a33</citedby><cites>FETCH-LOGICAL-c378t-f44404e6fb172f3531b63c62ec7afd8a467b082a85cfa9b267abc5ea234a28a33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S1090023304000358$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65534</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15727920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bashiruddin, John B.</creatorcontrib><creatorcontrib>Frey, Joachim</creatorcontrib><creatorcontrib>Königsson, Malin Heldtander</creatorcontrib><creatorcontrib>Johansson, Karl-Erik</creatorcontrib><creatorcontrib>Hotzel, Helmut</creatorcontrib><creatorcontrib>Diller, Roland</creatorcontrib><creatorcontrib>de Santis, Paola</creatorcontrib><creatorcontrib>Botelho, Ana</creatorcontrib><creatorcontrib>Ayling, Roger D.</creatorcontrib><creatorcontrib>Nicholas, Robin A.J.</creatorcontrib><creatorcontrib>Thiaucourt, François</creatorcontrib><creatorcontrib>Sachse, Konrad</creatorcontrib><title>Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial</title><title>The veterinary journal (1997)</title><addtitle>Vet J</addtitle><description>Diagnostic differentiation between the ruminant pathogens
Mycoplasma agalactiae and
Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for
M. agalactiae and three systems for
M. bovis on a total of 41 strains of the two
Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three
M. bovis strains from small ruminants and two
M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.</description><subject>accuracy</subject><subject>animal diseases</subject><subject>Animals</subject><subject>Cattle</subject><subject>Cattle Diseases - epidemiology</subject><subject>Cattle Diseases - microbiology</subject><subject>diagnostic techniques</subject><subject>disease diagnosis</subject><subject>DNA Primers</subject><subject>Europe - epidemiology</subject><subject>Identification</subject><subject>Mycoplasma agalactiae</subject><subject>Mycoplasma agalactiae - classification</subject><subject>Mycoplasma agalactiae - genetics</subject><subject>Mycoplasma agalactiae - isolation & purification</subject><subject>Mycoplasma bovis</subject><subject>Mycoplasma bovis - classification</subject><subject>Mycoplasma bovis - genetics</subject><subject>Mycoplasma bovis - isolation & purification</subject><subject>Mycoplasma Infections - epidemiology</subject><subject>Mycoplasma Infections - microbiology</subject><subject>Mycoplasma Infections - veterinary</subject><subject>mycoplasmosis</subject><subject>pathogen identification</subject><subject>Polymerase chain reaction</subject><subject>Polymerase Chain Reaction - standards</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>Predictive Value of Tests</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>RNA, Ribosomal, 23S - genetics</subject><subject>ruminants</subject><subject>Sensitivity and Specificity</subject><subject>Specificity</subject><subject>strains</subject><subject>test sensitivity</subject><subject>test specificity</subject><issn>1090-0233</issn><issn>1532-2971</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc9u1DAQxiMEoqXwAhzAp96y-F_iBHGpVoUiFYGAnq2JMy5eJfFieyPtI_DWOM0KOCGNZGvm930azVcULxndMMrqN7tNmnfDhlMqN5Tlah4V56wSvOStYo_zn7a0pFyIs-JZjDtKaSslf1qcsUpx1XJ6Xvy6nmE4QHJ-It6SL9uvJB5jwjES6wNJP5C4HqfkrDMrBVNPemcthqX9R_npaPx-gDgCgXsYwOQZPsD_TDo_u_iWXBHjhwE6H7J8RpKCg-F58cTCEPHF6b0o7t5ff9_elLefP3zcXt2WRqgmlVZKSSXWtmOKW1EJ1tXC1ByNAts3IGvV0YZDUxkLbcdrBZ2pELiQwBsQ4qK4XH33wf88YEx6dNFg3mdCf4i6VrJqFVUZ5Ctogo8xoNX74EYIR82oXgLQO70EoJcANGW5mix6dXI_dCP2fyWni2fg9QpY8Brug4v67hunTORwmoaJxeLdSmC-wuww6GgcTgZ7F9Ak3Xv3vw1-A4LVozc</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Bashiruddin, John B.</creator><creator>Frey, Joachim</creator><creator>Königsson, Malin Heldtander</creator><creator>Johansson, Karl-Erik</creator><creator>Hotzel, Helmut</creator><creator>Diller, Roland</creator><creator>de Santis, Paola</creator><creator>Botelho, Ana</creator><creator>Ayling, Roger D.</creator><creator>Nicholas, Robin A.J.</creator><creator>Thiaucourt, François</creator><creator>Sachse, Konrad</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050301</creationdate><title>Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial</title><author>Bashiruddin, John B. ; Frey, Joachim ; Königsson, Malin Heldtander ; Johansson, Karl-Erik ; Hotzel, Helmut ; Diller, Roland ; de Santis, Paola ; Botelho, Ana ; Ayling, Roger D. ; Nicholas, Robin A.J. ; Thiaucourt, François ; Sachse, Konrad</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c378t-f44404e6fb172f3531b63c62ec7afd8a467b082a85cfa9b267abc5ea234a28a33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>accuracy</topic><topic>animal diseases</topic><topic>Animals</topic><topic>Cattle</topic><topic>Cattle Diseases - epidemiology</topic><topic>Cattle Diseases - microbiology</topic><topic>diagnostic techniques</topic><topic>disease diagnosis</topic><topic>DNA Primers</topic><topic>Europe - epidemiology</topic><topic>Identification</topic><topic>Mycoplasma agalactiae</topic><topic>Mycoplasma agalactiae - classification</topic><topic>Mycoplasma agalactiae - genetics</topic><topic>Mycoplasma agalactiae - isolation & purification</topic><topic>Mycoplasma bovis</topic><topic>Mycoplasma bovis - classification</topic><topic>Mycoplasma bovis - genetics</topic><topic>Mycoplasma bovis - isolation & purification</topic><topic>Mycoplasma Infections - epidemiology</topic><topic>Mycoplasma Infections - microbiology</topic><topic>Mycoplasma Infections - veterinary</topic><topic>mycoplasmosis</topic><topic>pathogen identification</topic><topic>Polymerase chain reaction</topic><topic>Polymerase Chain Reaction - standards</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>Predictive Value of Tests</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>RNA, Ribosomal, 23S - genetics</topic><topic>ruminants</topic><topic>Sensitivity and Specificity</topic><topic>Specificity</topic><topic>strains</topic><topic>test sensitivity</topic><topic>test specificity</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bashiruddin, John B.</creatorcontrib><creatorcontrib>Frey, Joachim</creatorcontrib><creatorcontrib>Königsson, Malin Heldtander</creatorcontrib><creatorcontrib>Johansson, Karl-Erik</creatorcontrib><creatorcontrib>Hotzel, Helmut</creatorcontrib><creatorcontrib>Diller, Roland</creatorcontrib><creatorcontrib>de Santis, Paola</creatorcontrib><creatorcontrib>Botelho, Ana</creatorcontrib><creatorcontrib>Ayling, Roger D.</creatorcontrib><creatorcontrib>Nicholas, Robin A.J.</creatorcontrib><creatorcontrib>Thiaucourt, François</creatorcontrib><creatorcontrib>Sachse, Konrad</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The veterinary journal (1997)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bashiruddin, John B.</au><au>Frey, Joachim</au><au>Königsson, Malin Heldtander</au><au>Johansson, Karl-Erik</au><au>Hotzel, Helmut</au><au>Diller, Roland</au><au>de Santis, Paola</au><au>Botelho, Ana</au><au>Ayling, Roger D.</au><au>Nicholas, Robin A.J.</au><au>Thiaucourt, François</au><au>Sachse, Konrad</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial</atitle><jtitle>The veterinary journal (1997)</jtitle><addtitle>Vet J</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>169</volume><issue>2</issue><spage>268</spage><epage>275</epage><pages>268-275</pages><issn>1090-0233</issn><eissn>1532-2971</eissn><abstract>Diagnostic differentiation between the ruminant pathogens
Mycoplasma agalactiae and
Mycoplasma bovis is known to be problematic when only conventional serological and biochemical tests are used. The main reason for this is that both agents share a considerable number of related proteins and common epitopes. DNA-based detection methods offer advantages in terms of specificity and sensitivity. However, there is an urgent need to compare currently used PCR assays because they target different genomic regions and, therefore, may perform differently. In the present work, five laboratories, which use PCR routinely, evaluated the specificity of four different PCR systems for
M. agalactiae and three systems for
M. bovis on a total of 41 strains of the two
Mycoplasma species including six previously unidentified strains. As the vast majority of PCR examinations (97.1% of all tests) correctly identified the strains the specificity of all seven detection systems appears to be high. In four cases, incorrect identification by conventional diagnostic methods was rectified by PCR. Isolates from non-typical hosts, i.e. three
M. bovis strains from small ruminants and two
M. agalactiae strains from cattle, were characterised by sequencing the 16S and part of the 23S ribosomal RNA genes.</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>15727920</pmid><doi>10.1016/j.tvjl.2004.01.018</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1090-0233 |
| ispartof | The veterinary journal (1997), 2005-03, Vol.169 (2), p.268-275 |
| issn | 1090-0233 1532-2971 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | accuracy animal diseases Animals Cattle Cattle Diseases - epidemiology Cattle Diseases - microbiology diagnostic techniques disease diagnosis DNA Primers Europe - epidemiology Identification Mycoplasma agalactiae Mycoplasma agalactiae - classification Mycoplasma agalactiae - genetics Mycoplasma agalactiae - isolation & purification Mycoplasma bovis Mycoplasma bovis - classification Mycoplasma bovis - genetics Mycoplasma bovis - isolation & purification Mycoplasma Infections - epidemiology Mycoplasma Infections - microbiology Mycoplasma Infections - veterinary mycoplasmosis pathogen identification Polymerase chain reaction Polymerase Chain Reaction - standards Polymerase Chain Reaction - veterinary Predictive Value of Tests RNA, Ribosomal, 16S - genetics RNA, Ribosomal, 23S - genetics ruminants Sensitivity and Specificity Specificity strains test sensitivity test specificity |
| title | Evaluation of PCR systems for the identification and differentiation of Mycoplasma agalactiae and Mycoplasma bovis: A collaborative trial |
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