Helicobacter spp. in Cats: Association between Infecting Species and Epithelial Proliferation within the Gastric Lamina Propria
The aim of this study was to determine whether there is an association between Helicobacter spp. infection of the feline stomach and the presence of gastric lesions and epithelial proliferation within the mucosa of this tissue. The study included 23 pet cats of both sexes and of varied age and breed...
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| Veröffentlicht in: | Journal of comparative pathology 2009-08, Vol.141 (2), p.127-134 |
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| creator | Takemura, L.S. Camargo, P.L. Alfieri, A.A. Bracarense, A.P.F.R.L. |
| description | The aim of this study was to determine whether there is an association between
Helicobacter spp. infection of the feline stomach and the presence of gastric lesions and epithelial proliferation within the mucosa of this tissue. The study included 23 pet cats of both sexes and of varied age and breed. Eighteen of these animals were clinically normal and five had a history of chronic vomiting. Samples of the mucosa of the pyloric antrum, corpus and fundus were collected by gastroscopy. The presence of
Helicobacter spp. was confirmed by polymerase chain reaction (PCR) or Warthin–Starry (WS) staining and the species of
Helicobacter was determined by PCR. Mucosal lesions were evaluated by examination of sections stained by haematoxylin and eosin (HE) and epithelial proliferation was determined by enumerating nucleolar organizer regions (AgNOR). In 20 (87%) cats the presence of
Helicobacter spp. was confirmed by both PCR and WS. There was no significant difference in colonization density between the different gastric regions.
H. heilmannii was the most frequently identified species (17 of 20 cats), and
H. felis was only identified in co-infection (two of 17 cats). One sample that was PCR positive to the genus level for
Helicobacter spp. was negative for the four individual species reactions. Histological changes in the lamina propria included mild mononuclear inflammatory infiltration, the presence of lymphoid follicles, fibrosis and glandular degeneration. These changes were most severe in the pyloric antrum. There was significant association between infection with gastric
Helicobacter spp. and the presence of lymphoid follicles (
P
=
0.03), and between infection and epithelial proliferation in the antrum (
P
<
0.01), corpus (
P
<
0.001) and fundus (
P
<
0.001). |
| doi_str_mv | 10.1016/j.jcpa.2009.01.002 |
| format | Article |
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Helicobacter spp. infection of the feline stomach and the presence of gastric lesions and epithelial proliferation within the mucosa of this tissue. The study included 23 pet cats of both sexes and of varied age and breed. Eighteen of these animals were clinically normal and five had a history of chronic vomiting. Samples of the mucosa of the pyloric antrum, corpus and fundus were collected by gastroscopy. The presence of
Helicobacter spp. was confirmed by polymerase chain reaction (PCR) or Warthin–Starry (WS) staining and the species of
Helicobacter was determined by PCR. Mucosal lesions were evaluated by examination of sections stained by haematoxylin and eosin (HE) and epithelial proliferation was determined by enumerating nucleolar organizer regions (AgNOR). In 20 (87%) cats the presence of
Helicobacter spp. was confirmed by both PCR and WS. There was no significant difference in colonization density between the different gastric regions.
H. heilmannii was the most frequently identified species (17 of 20 cats), and
H. felis was only identified in co-infection (two of 17 cats). One sample that was PCR positive to the genus level for
Helicobacter spp. was negative for the four individual species reactions. Histological changes in the lamina propria included mild mononuclear inflammatory infiltration, the presence of lymphoid follicles, fibrosis and glandular degeneration. These changes were most severe in the pyloric antrum. There was significant association between infection with gastric
Helicobacter spp. and the presence of lymphoid follicles (
P
=
0.03), and between infection and epithelial proliferation in the antrum (
P
<
0.01), corpus (
P
<
0.001) and fundus (
P
<
0.001).</description><identifier>ISSN: 0021-9975</identifier><identifier>EISSN: 1532-3129</identifier><identifier>DOI: 10.1016/j.jcpa.2009.01.002</identifier><identifier>PMID: 19446836</identifier><language>eng</language><publisher>England: Elsevier Ltd</publisher><subject>Animals ; Antigens, Nuclear - metabolism ; bacterial infections ; cat ; Cat Diseases - metabolism ; Cat Diseases - microbiology ; Cat Diseases - pathology ; Cats ; Cell Proliferation ; DNA, Bacterial - analysis ; epithelial cells ; Epithelial Cells - metabolism ; Epithelial Cells - microbiology ; Epithelial Cells - pathology ; epithelial proliferation ; Felis ; Female ; gastric lesions ; gastric mucosa ; Gastritis - metabolism ; Gastritis - microbiology ; Gastritis - pathology ; Gastritis - veterinary ; Helicobacter ; Helicobacter - genetics ; Helicobacter - isolation & purification ; Helicobacter Infections - metabolism ; Helicobacter Infections - microbiology ; Helicobacter Infections - pathology ; Helicobacter Infections - veterinary ; Helicobacter spp ; histopathology ; infection ; lesions (animal) ; Male ; Mucous Membrane - metabolism ; Mucous Membrane - microbiology ; Mucous Membrane - pathology ; pathogen identification ; Polymerase Chain Reaction - veterinary ; stomach ; Stomach - metabolism ; Stomach - microbiology ; Stomach - pathology</subject><ispartof>Journal of comparative pathology, 2009-08, Vol.141 (2), p.127-134</ispartof><rights>2009 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c409t-c4fb80e620e5b405ff5b4cde755f5dc6483af5405f8c84bb26c003f96480a2bf3</citedby><cites>FETCH-LOGICAL-c409t-c4fb80e620e5b405ff5b4cde755f5dc6483af5405f8c84bb26c003f96480a2bf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jcpa.2009.01.002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19446836$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Takemura, L.S.</creatorcontrib><creatorcontrib>Camargo, P.L.</creatorcontrib><creatorcontrib>Alfieri, A.A.</creatorcontrib><creatorcontrib>Bracarense, A.P.F.R.L.</creatorcontrib><title>Helicobacter spp. in Cats: Association between Infecting Species and Epithelial Proliferation within the Gastric Lamina Propria</title><title>Journal of comparative pathology</title><addtitle>J Comp Pathol</addtitle><description>The aim of this study was to determine whether there is an association between
Helicobacter spp. infection of the feline stomach and the presence of gastric lesions and epithelial proliferation within the mucosa of this tissue. The study included 23 pet cats of both sexes and of varied age and breed. Eighteen of these animals were clinically normal and five had a history of chronic vomiting. Samples of the mucosa of the pyloric antrum, corpus and fundus were collected by gastroscopy. The presence of
Helicobacter spp. was confirmed by polymerase chain reaction (PCR) or Warthin–Starry (WS) staining and the species of
Helicobacter was determined by PCR. Mucosal lesions were evaluated by examination of sections stained by haematoxylin and eosin (HE) and epithelial proliferation was determined by enumerating nucleolar organizer regions (AgNOR). In 20 (87%) cats the presence of
Helicobacter spp. was confirmed by both PCR and WS. There was no significant difference in colonization density between the different gastric regions.
H. heilmannii was the most frequently identified species (17 of 20 cats), and
H. felis was only identified in co-infection (two of 17 cats). One sample that was PCR positive to the genus level for
Helicobacter spp. was negative for the four individual species reactions. Histological changes in the lamina propria included mild mononuclear inflammatory infiltration, the presence of lymphoid follicles, fibrosis and glandular degeneration. These changes were most severe in the pyloric antrum. There was significant association between infection with gastric
Helicobacter spp. and the presence of lymphoid follicles (
P
=
0.03), and between infection and epithelial proliferation in the antrum (
P
<
0.01), corpus (
P
<
0.001) and fundus (
P
<
0.001).</description><subject>Animals</subject><subject>Antigens, Nuclear - metabolism</subject><subject>bacterial infections</subject><subject>cat</subject><subject>Cat Diseases - metabolism</subject><subject>Cat Diseases - microbiology</subject><subject>Cat Diseases - pathology</subject><subject>Cats</subject><subject>Cell Proliferation</subject><subject>DNA, Bacterial - analysis</subject><subject>epithelial cells</subject><subject>Epithelial Cells - metabolism</subject><subject>Epithelial Cells - microbiology</subject><subject>Epithelial Cells - pathology</subject><subject>epithelial proliferation</subject><subject>Felis</subject><subject>Female</subject><subject>gastric lesions</subject><subject>gastric mucosa</subject><subject>Gastritis - metabolism</subject><subject>Gastritis - microbiology</subject><subject>Gastritis - pathology</subject><subject>Gastritis - veterinary</subject><subject>Helicobacter</subject><subject>Helicobacter - genetics</subject><subject>Helicobacter - isolation & purification</subject><subject>Helicobacter Infections - metabolism</subject><subject>Helicobacter Infections - microbiology</subject><subject>Helicobacter Infections - pathology</subject><subject>Helicobacter Infections - veterinary</subject><subject>Helicobacter spp</subject><subject>histopathology</subject><subject>infection</subject><subject>lesions (animal)</subject><subject>Male</subject><subject>Mucous Membrane - metabolism</subject><subject>Mucous Membrane - microbiology</subject><subject>Mucous Membrane - pathology</subject><subject>pathogen identification</subject><subject>Polymerase Chain Reaction - veterinary</subject><subject>stomach</subject><subject>Stomach - metabolism</subject><subject>Stomach - microbiology</subject><subject>Stomach - pathology</subject><issn>0021-9975</issn><issn>1532-3129</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERbeFP8ABfOK2YezE-UBcqlW_pJWKVHq2HGdcvMo6wc624sRf70RZqTe4eCTPM68_HsY-CsgEiPLrLtvZ0WQSoMlAZADyDVsJlct1LmTzlq1oR6ybplKn7CylHQDUtZLv2KloiqKs83LF_t5g7-3QGjth5GkcM-4D35gpfeMXKQ3Wm8kPgbc4PSMGfhsc2smHR34_ovWYuAkdvxz99IuCTM9_xKH3DuMy9kz7lEdNfm3SFL3lW7P3wczcGL15z06c6RN-ONZz9nB1-XNzs97eXd9uLrZrW0Az0eraGrCUgKotQDlHxXZYKeVUZ8uizo1Tc6O2ddG2srQAuWuoAUa2Lj9nX5bcMQ6_D5gmvffJYt-bgMMh6bIqVFWJ-r-gBFFAmVcEygW0cUgpotP0nr2Jf7QAPfvROz370bMfDUKTDRr6dEw_tHvsXkeOQgj4vADODNo8Rp_0wz2dmVNgCSCAiO8LgfRdTx6jTuQhWOx8JDW6G_y_bvACadur7A</recordid><startdate>20090801</startdate><enddate>20090801</enddate><creator>Takemura, L.S.</creator><creator>Camargo, P.L.</creator><creator>Alfieri, A.A.</creator><creator>Bracarense, A.P.F.R.L.</creator><general>Elsevier Ltd</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>20090801</creationdate><title>Helicobacter spp. in Cats: Association between Infecting Species and Epithelial Proliferation within the Gastric Lamina Propria</title><author>Takemura, L.S. ; Camargo, P.L. ; Alfieri, A.A. ; Bracarense, A.P.F.R.L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c409t-c4fb80e620e5b405ff5b4cde755f5dc6483af5405f8c84bb26c003f96480a2bf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antigens, Nuclear - metabolism</topic><topic>bacterial infections</topic><topic>cat</topic><topic>Cat Diseases - metabolism</topic><topic>Cat Diseases - microbiology</topic><topic>Cat Diseases - pathology</topic><topic>Cats</topic><topic>Cell Proliferation</topic><topic>DNA, Bacterial - analysis</topic><topic>epithelial cells</topic><topic>Epithelial Cells - metabolism</topic><topic>Epithelial Cells - microbiology</topic><topic>Epithelial Cells - pathology</topic><topic>epithelial proliferation</topic><topic>Felis</topic><topic>Female</topic><topic>gastric lesions</topic><topic>gastric mucosa</topic><topic>Gastritis - metabolism</topic><topic>Gastritis - microbiology</topic><topic>Gastritis - pathology</topic><topic>Gastritis - veterinary</topic><topic>Helicobacter</topic><topic>Helicobacter - genetics</topic><topic>Helicobacter - isolation & purification</topic><topic>Helicobacter Infections - metabolism</topic><topic>Helicobacter Infections - microbiology</topic><topic>Helicobacter Infections - pathology</topic><topic>Helicobacter Infections - veterinary</topic><topic>Helicobacter spp</topic><topic>histopathology</topic><topic>infection</topic><topic>lesions (animal)</topic><topic>Male</topic><topic>Mucous Membrane - metabolism</topic><topic>Mucous Membrane - microbiology</topic><topic>Mucous Membrane - pathology</topic><topic>pathogen identification</topic><topic>Polymerase Chain Reaction - veterinary</topic><topic>stomach</topic><topic>Stomach - metabolism</topic><topic>Stomach - microbiology</topic><topic>Stomach - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Takemura, L.S.</creatorcontrib><creatorcontrib>Camargo, P.L.</creatorcontrib><creatorcontrib>Alfieri, A.A.</creatorcontrib><creatorcontrib>Bracarense, A.P.F.R.L.</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of comparative pathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Takemura, L.S.</au><au>Camargo, P.L.</au><au>Alfieri, A.A.</au><au>Bracarense, A.P.F.R.L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Helicobacter spp. in Cats: Association between Infecting Species and Epithelial Proliferation within the Gastric Lamina Propria</atitle><jtitle>Journal of comparative pathology</jtitle><addtitle>J Comp Pathol</addtitle><date>2009-08-01</date><risdate>2009</risdate><volume>141</volume><issue>2</issue><spage>127</spage><epage>134</epage><pages>127-134</pages><issn>0021-9975</issn><eissn>1532-3129</eissn><abstract>The aim of this study was to determine whether there is an association between
Helicobacter spp. infection of the feline stomach and the presence of gastric lesions and epithelial proliferation within the mucosa of this tissue. The study included 23 pet cats of both sexes and of varied age and breed. Eighteen of these animals were clinically normal and five had a history of chronic vomiting. Samples of the mucosa of the pyloric antrum, corpus and fundus were collected by gastroscopy. The presence of
Helicobacter spp. was confirmed by polymerase chain reaction (PCR) or Warthin–Starry (WS) staining and the species of
Helicobacter was determined by PCR. Mucosal lesions were evaluated by examination of sections stained by haematoxylin and eosin (HE) and epithelial proliferation was determined by enumerating nucleolar organizer regions (AgNOR). In 20 (87%) cats the presence of
Helicobacter spp. was confirmed by both PCR and WS. There was no significant difference in colonization density between the different gastric regions.
H. heilmannii was the most frequently identified species (17 of 20 cats), and
H. felis was only identified in co-infection (two of 17 cats). One sample that was PCR positive to the genus level for
Helicobacter spp. was negative for the four individual species reactions. Histological changes in the lamina propria included mild mononuclear inflammatory infiltration, the presence of lymphoid follicles, fibrosis and glandular degeneration. These changes were most severe in the pyloric antrum. There was significant association between infection with gastric
Helicobacter spp. and the presence of lymphoid follicles (
P
=
0.03), and between infection and epithelial proliferation in the antrum (
P
<
0.01), corpus (
P
<
0.001) and fundus (
P
<
0.001).</abstract><cop>England</cop><pub>Elsevier Ltd</pub><pmid>19446836</pmid><doi>10.1016/j.jcpa.2009.01.002</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
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| ispartof | Journal of comparative pathology, 2009-08, Vol.141 (2), p.127-134 |
| issn | 0021-9975 1532-3129 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antigens, Nuclear - metabolism bacterial infections cat Cat Diseases - metabolism Cat Diseases - microbiology Cat Diseases - pathology Cats Cell Proliferation DNA, Bacterial - analysis epithelial cells Epithelial Cells - metabolism Epithelial Cells - microbiology Epithelial Cells - pathology epithelial proliferation Felis Female gastric lesions gastric mucosa Gastritis - metabolism Gastritis - microbiology Gastritis - pathology Gastritis - veterinary Helicobacter Helicobacter - genetics Helicobacter - isolation & purification Helicobacter Infections - metabolism Helicobacter Infections - microbiology Helicobacter Infections - pathology Helicobacter Infections - veterinary Helicobacter spp histopathology infection lesions (animal) Male Mucous Membrane - metabolism Mucous Membrane - microbiology Mucous Membrane - pathology pathogen identification Polymerase Chain Reaction - veterinary stomach Stomach - metabolism Stomach - microbiology Stomach - pathology |
| title | Helicobacter spp. in Cats: Association between Infecting Species and Epithelial Proliferation within the Gastric Lamina Propria |
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