Amino acid analysis by micellar electrokinetic chromatography with laser-induced fluorescence detection: Application to nanolitre-volume biological samples from Arabidopsis thaliana and Myzus persicae

Amino acids were derivatised with 4‐fluoro‐7‐nitrobenzo‐2,1,3‐oxadiazol (NBD‐F), separated by micellar electrokinetic chromatography (MEKC), and detected by argon‐ion (488 nm) laser‐induced fluorescence. The optimised MEKC background electrolyte conditions were: 40 mM sodium cholate, 5 mM β‐cyclodex...

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Veröffentlicht in:	Electrophoresis 2005-02, Vol.26 (4-5), p.911-919
Hauptverfasser:	Zhu, Xunlin, Shaw, P. Nicholas, Pritchard, Jeremy, Newbury, John, Hunt, Emma J., Barrett, David A.
Format:	Artikel
Sprache:	eng
Schlagworte:	4-Chloro-7-nitrobenzofurazan - analogs & derivatives 4-Chloro-7-nitrobenzofurazan - chemistry Amino acids Amino Acids - analysis Amino Acids - isolation & purification Animals Aphids - chemistry Arabidopsis - chemistry Body Fluids - chemistry Chromatography, Micellar Electrokinetic Capillary - methods Honeydew Laser-induced fluorescence Lasers Micellar electrokinetic chromatography Nanotechnology - instrumentation Phloem sap Reproducibility of Results Spectrometry, Fluorescence
Online-Zugang:	Volltext
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