Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process
To investigate molecular effects of anti-sperm autoantibodies on fertilization, we previously established anti-mouse sperm-head auto-monoclonal antibodies (mAbs). Among the mAbs established, one mAb (named Ts4) recognized the sugar moiety of TEX101, a germ cell-marker glycoprotein. In the present st...
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| Veröffentlicht in: | Journal of Reproduction and Development 2009, Vol.55(3), pp.240-246 |
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| creator | Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan)) Yoshitake, H Maruyama, M Takamori, K Ogawa, H Hasegawa, A Araki, Y |
| description | To investigate molecular effects of anti-sperm autoantibodies on fertilization, we previously established anti-mouse sperm-head auto-monoclonal antibodies (mAbs). Among the mAbs established, one mAb (named Ts4) recognized the sugar moiety of TEX101, a germ cell-marker glycoprotein. In the present study, we examined the immunoreactivity of Ts4 in mouse spermatozoa and fertilized eggs during early embryogenesis to clarify the distribution of the Ts4-reactive antigen in the fertilization process. Similar to TES101 mAb (a specific probe for TEX101), immunopositive staining of Ts4 was observed on spermatocytes, spermatids and spermatozoa within the testis. In contrast to the results obtained with TES101 mAb, Ts4 reacted with the sperm acrosomal region within the cauda epididymis. A Western blot analysis of epididymal sperm extract revealed that Ts4 mainly detected two bands between 100 and 150 kDa, while Ts4 faintly detected a band corresponding to TEX101 at 38 kDa. In addition, Ts4-reactive molecules were observed in the growing early embryo after fertilization. Since Ts4-reactive antigen, potentially a carbohydrate chain, is only observed in reproduction-related areas such as the testis, epididymal sperm-head and early embryo, it is expected to have an effect on fertilization. Therefore, additional studies of this antigen may elucidate the molecular mechanisms underlying the reproductive process. |
| doi_str_mv | 10.1262/jrd.20156 |
| format | Article |
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Among the mAbs established, one mAb (named Ts4) recognized the sugar moiety of TEX101, a germ cell-marker glycoprotein. In the present study, we examined the immunoreactivity of Ts4 in mouse spermatozoa and fertilized eggs during early embryogenesis to clarify the distribution of the Ts4-reactive antigen in the fertilization process. Similar to TES101 mAb (a specific probe for TEX101), immunopositive staining of Ts4 was observed on spermatocytes, spermatids and spermatozoa within the testis. In contrast to the results obtained with TES101 mAb, Ts4 reacted with the sperm acrosomal region within the cauda epididymis. A Western blot analysis of epididymal sperm extract revealed that Ts4 mainly detected two bands between 100 and 150 kDa, while Ts4 faintly detected a band corresponding to TEX101 at 38 kDa. In addition, Ts4-reactive molecules were observed in the growing early embryo after fertilization. Since Ts4-reactive antigen, potentially a carbohydrate chain, is only observed in reproduction-related areas such as the testis, epididymal sperm-head and early embryo, it is expected to have an effect on fertilization. Therefore, additional studies of this antigen may elucidate the molecular mechanisms underlying the reproductive process.</description><identifier>ISSN: 0916-8818</identifier><identifier>EISSN: 1348-4400</identifier><identifier>DOI: 10.1262/jrd.20156</identifier><identifier>PMID: 19234371</identifier><language>eng</language><publisher>Japan: THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</publisher><subject>ANIMAL EMBRYOS ; Animals ; Antibodies, Monoclonal - analysis ; Antibodies, Monoclonal - chemistry ; Antibodies, Monoclonal - immunology ; ANTICORPS MONOCLONAL ; ANTICUERPOS MONOCLONALES ; ANTIGENE ; ANTIGENOS ; ANTIGENS ; Auto-antibody ; Autoantibodies - analysis ; Autoantibodies - chemistry ; Autoantibodies - immunology ; EMBRIONES ANIMALES ; EMBRYON ANIMAL ; Epitope Mapping ; ESPERMATOZOO ; FECONDATION ; FECUNDACION ; Female ; FERTILIZATION ; Fertilization - immunology ; Genitalia, Male - immunology ; Genitalia, Male - metabolism ; GLICOPROTEINAS ; GLYCOPROTEINE ; GLYCOPROTEINS ; Male ; MICE ; Mice, Inbred C57BL ; Mice, Inbred ICR ; Models, Biological ; MONOCLONAL ANTIBODIES ; Monoclonal antibody ; RATON ; SOURIS ; Sperm ; SPERMATOZOA ; Spermatozoa - immunology ; SPERMATOZOIDE ; TESTES ; TESTICULE ; TESTICULOS ; TEX101 ; Zygote - immunology ; Zygote - metabolism</subject><ispartof>Journal of Reproduction and Development, 2009, Vol.55(3), pp.240-246</ispartof><rights>2009 Society for Reproduction and Development</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c600t-81f8975540945e0cce42c8ae5578c82c96636b492c7e3223a17d69256b62310f3</citedby><cites>FETCH-LOGICAL-c600t-81f8975540945e0cce42c8ae5578c82c96636b492c7e3223a17d69256b62310f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,860,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19234371$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan))</creatorcontrib><creatorcontrib>Yoshitake, H</creatorcontrib><creatorcontrib>Maruyama, M</creatorcontrib><creatorcontrib>Takamori, K</creatorcontrib><creatorcontrib>Ogawa, H</creatorcontrib><creatorcontrib>Hasegawa, A</creatorcontrib><creatorcontrib>Araki, Y</creatorcontrib><title>Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process</title><title>Journal of Reproduction and Development</title><addtitle>J. Reprod. Dev.</addtitle><description>To investigate molecular effects of anti-sperm autoantibodies on fertilization, we previously established anti-mouse sperm-head auto-monoclonal antibodies (mAbs). Among the mAbs established, one mAb (named Ts4) recognized the sugar moiety of TEX101, a germ cell-marker glycoprotein. In the present study, we examined the immunoreactivity of Ts4 in mouse spermatozoa and fertilized eggs during early embryogenesis to clarify the distribution of the Ts4-reactive antigen in the fertilization process. Similar to TES101 mAb (a specific probe for TEX101), immunopositive staining of Ts4 was observed on spermatocytes, spermatids and spermatozoa within the testis. In contrast to the results obtained with TES101 mAb, Ts4 reacted with the sperm acrosomal region within the cauda epididymis. A Western blot analysis of epididymal sperm extract revealed that Ts4 mainly detected two bands between 100 and 150 kDa, while Ts4 faintly detected a band corresponding to TEX101 at 38 kDa. In addition, Ts4-reactive molecules were observed in the growing early embryo after fertilization. Since Ts4-reactive antigen, potentially a carbohydrate chain, is only observed in reproduction-related areas such as the testis, epididymal sperm-head and early embryo, it is expected to have an effect on fertilization. Therefore, additional studies of this antigen may elucidate the molecular mechanisms underlying the reproductive process.</description><subject>ANIMAL EMBRYOS</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - analysis</subject><subject>Antibodies, Monoclonal - chemistry</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>ANTICORPS MONOCLONAL</subject><subject>ANTICUERPOS MONOCLONALES</subject><subject>ANTIGENE</subject><subject>ANTIGENOS</subject><subject>ANTIGENS</subject><subject>Auto-antibody</subject><subject>Autoantibodies - analysis</subject><subject>Autoantibodies - chemistry</subject><subject>Autoantibodies - immunology</subject><subject>EMBRIONES ANIMALES</subject><subject>EMBRYON ANIMAL</subject><subject>Epitope Mapping</subject><subject>ESPERMATOZOO</subject><subject>FECONDATION</subject><subject>FECUNDACION</subject><subject>Female</subject><subject>FERTILIZATION</subject><subject>Fertilization - immunology</subject><subject>Genitalia, Male - immunology</subject><subject>Genitalia, Male - metabolism</subject><subject>GLICOPROTEINAS</subject><subject>GLYCOPROTEINE</subject><subject>GLYCOPROTEINS</subject><subject>Male</subject><subject>MICE</subject><subject>Mice, Inbred C57BL</subject><subject>Mice, Inbred ICR</subject><subject>Models, Biological</subject><subject>MONOCLONAL ANTIBODIES</subject><subject>Monoclonal antibody</subject><subject>RATON</subject><subject>SOURIS</subject><subject>Sperm</subject><subject>SPERMATOZOA</subject><subject>Spermatozoa - immunology</subject><subject>SPERMATOZOIDE</subject><subject>TESTES</subject><subject>TESTICULE</subject><subject>TESTICULOS</subject><subject>TEX101</subject><subject>Zygote - immunology</subject><subject>Zygote - metabolism</subject><issn>0916-8818</issn><issn>1348-4400</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi1ERbeFAz8A5BNSJVLGn0lOFSpfrSrBoZwtxztpvUriYDuH8uvrZlfliGTZh3n8jGZeQt4yOGdc80-7uD3nwJR-QTZMyKaSEuAl2UDLdNU0rDkmJyntAARXWr4ix6zlQoqabcj0xaccfbdkHyYaejqGAd0y2Ehx9jnMSPsQ6W2SH6mdysm-SjPGkdolh2oMU3BDmOywlrqwfaB-ovm-fMOY_eD_2tU8x-AwpdfkqLdDwjeH95T8_vb19vJHdfPz-9Xl55vKaYBcNaxv2lopCa1UCM6h5K6xqFTduIa7VmuhO9lyV6PgXFhWb3Vbhus0Fwx6cUo-7L2l758FUzajTw6HwU4YlmR0LaViUvwX5MCF4iALeLYHXQwpRezNHP1o44NhYJ5SMCUFs6ZQ2PcH6dKNuP1HHtZegIs9sEvZ3uEzYMvK3ICrSikjnq5V-Vxx9zYanIrh3d7Q22DsXfTJXP_iAC2ArGshHgHRJKIA</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan))</creator><creator>Yoshitake, H</creator><creator>Maruyama, M</creator><creator>Takamori, K</creator><creator>Ogawa, H</creator><creator>Hasegawa, A</creator><creator>Araki, Y</creator><general>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090601</creationdate><title>Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process</title><author>Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan)) ; Yoshitake, H ; Maruyama, M ; Takamori, K ; Ogawa, H ; Hasegawa, A ; Araki, Y</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c600t-81f8975540945e0cce42c8ae5578c82c96636b492c7e3223a17d69256b62310f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>ANIMAL EMBRYOS</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - analysis</topic><topic>Antibodies, Monoclonal - chemistry</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>ANTICORPS MONOCLONAL</topic><topic>ANTICUERPOS MONOCLONALES</topic><topic>ANTIGENE</topic><topic>ANTIGENOS</topic><topic>ANTIGENS</topic><topic>Auto-antibody</topic><topic>Autoantibodies - analysis</topic><topic>Autoantibodies - chemistry</topic><topic>Autoantibodies - immunology</topic><topic>EMBRIONES ANIMALES</topic><topic>EMBRYON ANIMAL</topic><topic>Epitope Mapping</topic><topic>ESPERMATOZOO</topic><topic>FECONDATION</topic><topic>FECUNDACION</topic><topic>Female</topic><topic>FERTILIZATION</topic><topic>Fertilization - immunology</topic><topic>Genitalia, Male - immunology</topic><topic>Genitalia, Male - metabolism</topic><topic>GLICOPROTEINAS</topic><topic>GLYCOPROTEINE</topic><topic>GLYCOPROTEINS</topic><topic>Male</topic><topic>MICE</topic><topic>Mice, Inbred C57BL</topic><topic>Mice, Inbred ICR</topic><topic>Models, Biological</topic><topic>MONOCLONAL ANTIBODIES</topic><topic>Monoclonal antibody</topic><topic>RATON</topic><topic>SOURIS</topic><topic>Sperm</topic><topic>SPERMATOZOA</topic><topic>Spermatozoa - immunology</topic><topic>SPERMATOZOIDE</topic><topic>TESTES</topic><topic>TESTICULE</topic><topic>TESTICULOS</topic><topic>TEX101</topic><topic>Zygote - immunology</topic><topic>Zygote - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan))</creatorcontrib><creatorcontrib>Yoshitake, H</creatorcontrib><creatorcontrib>Maruyama, M</creatorcontrib><creatorcontrib>Takamori, K</creatorcontrib><creatorcontrib>Ogawa, H</creatorcontrib><creatorcontrib>Hasegawa, A</creatorcontrib><creatorcontrib>Araki, Y</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of Reproduction and Development</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shirai, Y.(Juntendo Univ., Urayasu, Chiba (Japan))</au><au>Yoshitake, H</au><au>Maruyama, M</au><au>Takamori, K</au><au>Ogawa, H</au><au>Hasegawa, A</au><au>Araki, Y</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process</atitle><jtitle>Journal of Reproduction and Development</jtitle><addtitle>J. Reprod. Dev.</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>55</volume><issue>3</issue><spage>240</spage><epage>246</epage><pages>240-246</pages><issn>0916-8818</issn><eissn>1348-4400</eissn><abstract>To investigate molecular effects of anti-sperm autoantibodies on fertilization, we previously established anti-mouse sperm-head auto-monoclonal antibodies (mAbs). Among the mAbs established, one mAb (named Ts4) recognized the sugar moiety of TEX101, a germ cell-marker glycoprotein. In the present study, we examined the immunoreactivity of Ts4 in mouse spermatozoa and fertilized eggs during early embryogenesis to clarify the distribution of the Ts4-reactive antigen in the fertilization process. Similar to TES101 mAb (a specific probe for TEX101), immunopositive staining of Ts4 was observed on spermatocytes, spermatids and spermatozoa within the testis. In contrast to the results obtained with TES101 mAb, Ts4 reacted with the sperm acrosomal region within the cauda epididymis. A Western blot analysis of epididymal sperm extract revealed that Ts4 mainly detected two bands between 100 and 150 kDa, while Ts4 faintly detected a band corresponding to TEX101 at 38 kDa. In addition, Ts4-reactive molecules were observed in the growing early embryo after fertilization. Since Ts4-reactive antigen, potentially a carbohydrate chain, is only observed in reproduction-related areas such as the testis, epididymal sperm-head and early embryo, it is expected to have an effect on fertilization. Therefore, additional studies of this antigen may elucidate the molecular mechanisms underlying the reproductive process.</abstract><cop>Japan</cop><pub>THE SOCIETY FOR REPRODUCTION AND DEVELOPMENT</pub><pmid>19234371</pmid><doi>10.1262/jrd.20156</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| source | J-STAGE Free; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | ANIMAL EMBRYOS Animals Antibodies, Monoclonal - analysis Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - immunology ANTICORPS MONOCLONAL ANTICUERPOS MONOCLONALES ANTIGENE ANTIGENOS ANTIGENS Auto-antibody Autoantibodies - analysis Autoantibodies - chemistry Autoantibodies - immunology EMBRIONES ANIMALES EMBRYON ANIMAL Epitope Mapping ESPERMATOZOO FECONDATION FECUNDACION Female FERTILIZATION Fertilization - immunology Genitalia, Male - immunology Genitalia, Male - metabolism GLICOPROTEINAS GLYCOPROTEINE GLYCOPROTEINS Male MICE Mice, Inbred C57BL Mice, Inbred ICR Models, Biological MONOCLONAL ANTIBODIES Monoclonal antibody RATON SOURIS Sperm SPERMATOZOA Spermatozoa - immunology SPERMATOZOIDE TESTES TESTICULE TESTICULOS TEX101 Zygote - immunology Zygote - metabolism |
| title | Distribution of molecular epitope for Ts4, an anti-sperm auto-monoclonal antibody in the fertilization process |
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