Expression Analysis and Characteristics of Profilin Gene from Silkworm, Bombyx mori

A recombinant Bombyx mori profilin protein (rBmPFN) was overexpressed in Escherichia coli BL21. Purified rBmPFN was used to generate anti-BmPFN polyclonal antibody, which were used to determine the subcellular localization of BmPFN. Immunostaining indicated that profilin can be found in both the nuc...

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Veröffentlicht in:	Applied biochemistry and biotechnology 2009-07, Vol.158 (1), p.59-71
Hauptverfasser:	Nie, Zuoming, Xu, Jiangtao, Chen, Jian, Lv, Zhengbing, Wang, Dan, Sheng, Qing, Wu, Yi, Wang, Xuedong, Wu, Xiangfu, Zhang, Yaozhou
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies - immunology Biochemistry Biological and medical sciences Biotechnology Bombyx - cytology Bombyx - genetics Bombyx - growth & development Bombyx mori cell nucleus Cellular biology Chemistry Chemistry and Materials Science Computational Biology cytoplasm DNA, Complementary - genetics E coli Escherichia coli Escherichia coli - genetics Evolution, Molecular Fundamental and applied biological sciences. Psychology Gene Expression Gene Expression Regulation Gene Expression Regulation, Developmental gene overexpression Humans Intracellular Space - metabolism Larvae Molecular Sequence Data Phylogeny polyclonal antibodies profilin Profilins - chemistry Profilins - genetics Profilins - immunology Profilins - metabolism Protein Transport Proteins pupal development reverse transcriptase polymerase chain reaction silk glands silk proteins silkworms tissue distribution Western blotting Worms
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