Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2
The Epstein–Barr virus (EBV)-encoded latency protein Epstein–Barr nuclear antigen 2 (EBNA2) is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. In a previous study, we demonstrated that EBNA2 interacts with signal transducer and activator of transcriptio...
Gespeichert in:
| Veröffentlicht in: | Biological & Pharmaceutical Bulletin 2009/07/01, Vol.32(7), pp.1283-1285 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1285 |
|---|---|
| container_issue | 7 |
| container_start_page | 1283 |
| container_title | Biological & Pharmaceutical Bulletin |
| container_volume | 32 |
| creator | Ikeda, Osamu Togi, Sumihito Kamitani, Shinya Muromoto, Ryuta Sekine, Yuichi Nanbo, Asuka Fujimuro, Masahiro Matsuda, Tadashi |
| description | The Epstein–Barr virus (EBV)-encoded latency protein Epstein–Barr nuclear antigen 2 (EBNA2) is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. In a previous study, we demonstrated that EBNA2 interacts with signal transducer and activator of transcription 3 (STAT3), a signal transducer for an interleukin (IL)-6 family cytokine, and enhances its transcriptional activity. Here, we show that overexpression of a corepressor, silencing mediator of retinoic acid and thyroid hormone receptor (SMRT), decreases the EBNA2-mediated enhanced STAT3 activation. Furthermore, small-interfering RNA-mediated reduction of endogenous SMRT expression augments the EBNA2-mediated enhanced STAT3 activation. Importantly, EBNA2 reduces interactions between STAT3 and SMRT. These data demonstrate that EBNA2 acts as a transcriptional coactivator of STAT3 by influencing the SMRT corepressor complex. |
| doi_str_mv | 10.1248/bpb.32.1283 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67442804</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>20797130</sourcerecordid><originalsourceid>FETCH-LOGICAL-c764t-4dd07a704d923ff01e93f2ad6730cdc270baf120f1e797cf297550044acc51e73</originalsourceid><addsrcrecordid>eNqFks9u1DAQxiMEoqVw4o4sIXFBKf6XeHNjKVuKVEBqF66W40x2vcraqe1U2hvvwLPxAjwJzmZLpV6QJXvk-c33jezJspcEnxLKZ-_qvj5lNMUz9ig7JoyLvKCkeJwd44rM8pIUs6PsWQgbjLHAlD3NjkhVCMKq6jj7fW06sNrYFfoCjVHReeRadAXRWGc0mmvTIGUbtFzvvEvxhfNbZyERGvqRvoLV0KkIAS3sWlkNTSqK5lZF4-yodW1WVnVo6ZUNzaDB7_UOzGS3z2lv-n0NQ_UOLfoQwdg_P399UN6jH8YPIf8I3twmgwfJr4PuQHk0t9GswCL6PHvSqi7Ai8N5kn0_XyzPLvLLb58-n80vcy1KHnPeNFgogXlTUda2mEDFWqqaUjCsG00FrlVLKG4JiErollaiKDDmXGldpDt2kr2ZdHvvbgYIUW5N0NB1yoIbgiwF53SG-X9BipMBYTiBrx-AGzf49H5BEs4rJtIqE_V2orR3IXhoZe_NVvmdJFiOQyHTUEhG5TgUiX510BzqLTT37GEKEnA-ASlrtOqc7YyFe2cdRG1c51KbuJIYs9SuxLTcy49bwdJ74tHp_SS0CVGt4J-T8tGkL7rrSkzbvvoupdfKS7DsLz095Cw</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1449373736</pqid></control><display><type>article</type><title>Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2</title><source>J-STAGE Free</source><source>MEDLINE</source><source>Free Full-Text Journals in Chemistry</source><source>EZB Electronic Journals Library</source><creator>Ikeda, Osamu ; Togi, Sumihito ; Kamitani, Shinya ; Muromoto, Ryuta ; Sekine, Yuichi ; Nanbo, Asuka ; Fujimuro, Masahiro ; Matsuda, Tadashi</creator><creatorcontrib>Ikeda, Osamu ; Togi, Sumihito ; Kamitani, Shinya ; Muromoto, Ryuta ; Sekine, Yuichi ; Nanbo, Asuka ; Fujimuro, Masahiro ; Matsuda, Tadashi ; University of Yamanashi ; aDepartment of Immunology ; Interdisciplinary Graduate School of Medicine and Engineering ; bDepartment of Molecular Cell Biology ; Graduate School of Pharmaceutical Sciences ; Hokkaido University</creatorcontrib><description>The Epstein–Barr virus (EBV)-encoded latency protein Epstein–Barr nuclear antigen 2 (EBNA2) is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. In a previous study, we demonstrated that EBNA2 interacts with signal transducer and activator of transcription 3 (STAT3), a signal transducer for an interleukin (IL)-6 family cytokine, and enhances its transcriptional activity. Here, we show that overexpression of a corepressor, silencing mediator of retinoic acid and thyroid hormone receptor (SMRT), decreases the EBNA2-mediated enhanced STAT3 activation. Furthermore, small-interfering RNA-mediated reduction of endogenous SMRT expression augments the EBNA2-mediated enhanced STAT3 activation. Importantly, EBNA2 reduces interactions between STAT3 and SMRT. These data demonstrate that EBNA2 acts as a transcriptional coactivator of STAT3 by influencing the SMRT corepressor complex.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.32.1283</identifier><identifier>PMID: 19571399</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>Antibodies, Monoclonal - pharmacology ; DNA-Binding Proteins - biosynthesis ; DNA-Binding Proteins - metabolism ; Epstein-Barr virus ; Epstein-Barr Virus Nuclear Antigens - metabolism ; Epstein–Barr nuclear antigen 2 ; HeLa Cells ; Humans ; Immunoblotting ; Immunoprecipitation ; Interleukin-6 - biosynthesis ; Luciferases - genetics ; Nuclear Receptor Co-Repressor 2 ; Repressor Proteins - biosynthesis ; Repressor Proteins - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; RNA, Small Interfering - genetics ; SMRT ; STAT3 ; STAT3 Transcription Factor - biosynthesis ; STAT3 Transcription Factor - genetics ; Transcriptional Activation ; Transfection ; Viral Proteins - metabolism</subject><ispartof>Biological and Pharmaceutical Bulletin, 2009/07/01, Vol.32(7), pp.1283-1285</ispartof><rights>2009 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c764t-4dd07a704d923ff01e93f2ad6730cdc270baf120f1e797cf297550044acc51e73</citedby><cites>FETCH-LOGICAL-c764t-4dd07a704d923ff01e93f2ad6730cdc270baf120f1e797cf297550044acc51e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>315,781,785,1884,27928,27929</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19571399$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ikeda, Osamu</creatorcontrib><creatorcontrib>Togi, Sumihito</creatorcontrib><creatorcontrib>Kamitani, Shinya</creatorcontrib><creatorcontrib>Muromoto, Ryuta</creatorcontrib><creatorcontrib>Sekine, Yuichi</creatorcontrib><creatorcontrib>Nanbo, Asuka</creatorcontrib><creatorcontrib>Fujimuro, Masahiro</creatorcontrib><creatorcontrib>Matsuda, Tadashi</creatorcontrib><creatorcontrib>University of Yamanashi</creatorcontrib><creatorcontrib>aDepartment of Immunology</creatorcontrib><creatorcontrib>Interdisciplinary Graduate School of Medicine and Engineering</creatorcontrib><creatorcontrib>bDepartment of Molecular Cell Biology</creatorcontrib><creatorcontrib>Graduate School of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Hokkaido University</creatorcontrib><title>Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2</title><title>Biological & Pharmaceutical Bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>The Epstein–Barr virus (EBV)-encoded latency protein Epstein–Barr nuclear antigen 2 (EBNA2) is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. In a previous study, we demonstrated that EBNA2 interacts with signal transducer and activator of transcription 3 (STAT3), a signal transducer for an interleukin (IL)-6 family cytokine, and enhances its transcriptional activity. Here, we show that overexpression of a corepressor, silencing mediator of retinoic acid and thyroid hormone receptor (SMRT), decreases the EBNA2-mediated enhanced STAT3 activation. Furthermore, small-interfering RNA-mediated reduction of endogenous SMRT expression augments the EBNA2-mediated enhanced STAT3 activation. Importantly, EBNA2 reduces interactions between STAT3 and SMRT. These data demonstrate that EBNA2 acts as a transcriptional coactivator of STAT3 by influencing the SMRT corepressor complex.</description><subject>Antibodies, Monoclonal - pharmacology</subject><subject>DNA-Binding Proteins - biosynthesis</subject><subject>DNA-Binding Proteins - metabolism</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Nuclear Antigens - metabolism</subject><subject>Epstein–Barr nuclear antigen 2</subject><subject>HeLa Cells</subject><subject>Humans</subject><subject>Immunoblotting</subject><subject>Immunoprecipitation</subject><subject>Interleukin-6 - biosynthesis</subject><subject>Luciferases - genetics</subject><subject>Nuclear Receptor Co-Repressor 2</subject><subject>Repressor Proteins - biosynthesis</subject><subject>Repressor Proteins - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Small Interfering - genetics</subject><subject>SMRT</subject><subject>STAT3</subject><subject>STAT3 Transcription Factor - biosynthesis</subject><subject>STAT3 Transcription Factor - genetics</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><subject>Viral Proteins - metabolism</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFks9u1DAQxiMEoqVw4o4sIXFBKf6XeHNjKVuKVEBqF66W40x2vcraqe1U2hvvwLPxAjwJzmZLpV6QJXvk-c33jezJspcEnxLKZ-_qvj5lNMUz9ig7JoyLvKCkeJwd44rM8pIUs6PsWQgbjLHAlD3NjkhVCMKq6jj7fW06sNrYFfoCjVHReeRadAXRWGc0mmvTIGUbtFzvvEvxhfNbZyERGvqRvoLV0KkIAS3sWlkNTSqK5lZF4-yodW1WVnVo6ZUNzaDB7_UOzGS3z2lv-n0NQ_UOLfoQwdg_P399UN6jH8YPIf8I3twmgwfJr4PuQHk0t9GswCL6PHvSqi7Ai8N5kn0_XyzPLvLLb58-n80vcy1KHnPeNFgogXlTUda2mEDFWqqaUjCsG00FrlVLKG4JiErollaiKDDmXGldpDt2kr2ZdHvvbgYIUW5N0NB1yoIbgiwF53SG-X9BipMBYTiBrx-AGzf49H5BEs4rJtIqE_V2orR3IXhoZe_NVvmdJFiOQyHTUEhG5TgUiX510BzqLTT37GEKEnA-ASlrtOqc7YyFe2cdRG1c51KbuJIYs9SuxLTcy49bwdJ74tHp_SS0CVGt4J-T8tGkL7rrSkzbvvoupdfKS7DsLz095Cw</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Ikeda, Osamu</creator><creator>Togi, Sumihito</creator><creator>Kamitani, Shinya</creator><creator>Muromoto, Ryuta</creator><creator>Sekine, Yuichi</creator><creator>Nanbo, Asuka</creator><creator>Fujimuro, Masahiro</creator><creator>Matsuda, Tadashi</creator><general>The Pharmaceutical Society of Japan</general><general>Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2</title><author>Ikeda, Osamu ; Togi, Sumihito ; Kamitani, Shinya ; Muromoto, Ryuta ; Sekine, Yuichi ; Nanbo, Asuka ; Fujimuro, Masahiro ; Matsuda, Tadashi</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c764t-4dd07a704d923ff01e93f2ad6730cdc270baf120f1e797cf297550044acc51e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Antibodies, Monoclonal - pharmacology</topic><topic>DNA-Binding Proteins - biosynthesis</topic><topic>DNA-Binding Proteins - metabolism</topic><topic>Epstein-Barr virus</topic><topic>Epstein-Barr Virus Nuclear Antigens - metabolism</topic><topic>Epstein–Barr nuclear antigen 2</topic><topic>HeLa Cells</topic><topic>Humans</topic><topic>Immunoblotting</topic><topic>Immunoprecipitation</topic><topic>Interleukin-6 - biosynthesis</topic><topic>Luciferases - genetics</topic><topic>Nuclear Receptor Co-Repressor 2</topic><topic>Repressor Proteins - biosynthesis</topic><topic>Repressor Proteins - metabolism</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Small Interfering - genetics</topic><topic>SMRT</topic><topic>STAT3</topic><topic>STAT3 Transcription Factor - biosynthesis</topic><topic>STAT3 Transcription Factor - genetics</topic><topic>Transcriptional Activation</topic><topic>Transfection</topic><topic>Viral Proteins - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ikeda, Osamu</creatorcontrib><creatorcontrib>Togi, Sumihito</creatorcontrib><creatorcontrib>Kamitani, Shinya</creatorcontrib><creatorcontrib>Muromoto, Ryuta</creatorcontrib><creatorcontrib>Sekine, Yuichi</creatorcontrib><creatorcontrib>Nanbo, Asuka</creatorcontrib><creatorcontrib>Fujimuro, Masahiro</creatorcontrib><creatorcontrib>Matsuda, Tadashi</creatorcontrib><creatorcontrib>University of Yamanashi</creatorcontrib><creatorcontrib>aDepartment of Immunology</creatorcontrib><creatorcontrib>Interdisciplinary Graduate School of Medicine and Engineering</creatorcontrib><creatorcontrib>bDepartment of Molecular Cell Biology</creatorcontrib><creatorcontrib>Graduate School of Pharmaceutical Sciences</creatorcontrib><creatorcontrib>Hokkaido University</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & Pharmaceutical Bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ikeda, Osamu</au><au>Togi, Sumihito</au><au>Kamitani, Shinya</au><au>Muromoto, Ryuta</au><au>Sekine, Yuichi</au><au>Nanbo, Asuka</au><au>Fujimuro, Masahiro</au><au>Matsuda, Tadashi</au><aucorp>University of Yamanashi</aucorp><aucorp>aDepartment of Immunology</aucorp><aucorp>Interdisciplinary Graduate School of Medicine and Engineering</aucorp><aucorp>bDepartment of Molecular Cell Biology</aucorp><aucorp>Graduate School of Pharmaceutical Sciences</aucorp><aucorp>Hokkaido University</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2</atitle><jtitle>Biological & Pharmaceutical Bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>32</volume><issue>7</issue><spage>1283</spage><epage>1285</epage><pages>1283-1285</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>The Epstein–Barr virus (EBV)-encoded latency protein Epstein–Barr nuclear antigen 2 (EBNA2) is a nuclear transcriptional activator that is essential for EBV-induced cellular transformation. In a previous study, we demonstrated that EBNA2 interacts with signal transducer and activator of transcription 3 (STAT3), a signal transducer for an interleukin (IL)-6 family cytokine, and enhances its transcriptional activity. Here, we show that overexpression of a corepressor, silencing mediator of retinoic acid and thyroid hormone receptor (SMRT), decreases the EBNA2-mediated enhanced STAT3 activation. Furthermore, small-interfering RNA-mediated reduction of endogenous SMRT expression augments the EBNA2-mediated enhanced STAT3 activation. Importantly, EBNA2 reduces interactions between STAT3 and SMRT. These data demonstrate that EBNA2 acts as a transcriptional coactivator of STAT3 by influencing the SMRT corepressor complex.</abstract><cop>Japan</cop><pub>The Pharmaceutical Society of Japan</pub><pmid>19571399</pmid><doi>10.1248/bpb.32.1283</doi><tpages>3</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0918-6158 |
| ispartof | Biological and Pharmaceutical Bulletin, 2009/07/01, Vol.32(7), pp.1283-1285 |
| issn | 0918-6158 1347-5215 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67442804 |
| source | J-STAGE Free; MEDLINE; Free Full-Text Journals in Chemistry; EZB Electronic Journals Library |
| subjects | Antibodies, Monoclonal - pharmacology DNA-Binding Proteins - biosynthesis DNA-Binding Proteins - metabolism Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens - metabolism Epstein–Barr nuclear antigen 2 HeLa Cells Humans Immunoblotting Immunoprecipitation Interleukin-6 - biosynthesis Luciferases - genetics Nuclear Receptor Co-Repressor 2 Repressor Proteins - biosynthesis Repressor Proteins - metabolism Reverse Transcriptase Polymerase Chain Reaction RNA, Small Interfering - genetics SMRT STAT3 STAT3 Transcription Factor - biosynthesis STAT3 Transcription Factor - genetics Transcriptional Activation Transfection Viral Proteins - metabolism |
| title | Silencing Mediator of Retinoic Acid and Thyroid Hormone Receptor Regulates Enhanced Activation of Signal Transducer and Activator of Transcription 3 by Epstein–Barr Virus-Derived Epstein–Barr Nuclear Antigen 2 |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-16T21%3A43%3A01IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Silencing%20Mediator%20of%20Retinoic%20Acid%20and%20Thyroid%20Hormone%20Receptor%20Regulates%20Enhanced%20Activation%20of%20Signal%20Transducer%20and%20Activator%20of%20Transcription%203%20by%20Epstein%E2%80%93Barr%20Virus-Derived%20Epstein%E2%80%93Barr%20Nuclear%20Antigen%202&rft.jtitle=Biological%20&%20Pharmaceutical%20Bulletin&rft.au=Ikeda,%20Osamu&rft.aucorp=University%20of%20Yamanashi&rft.date=2009-07-01&rft.volume=32&rft.issue=7&rft.spage=1283&rft.epage=1285&rft.pages=1283-1285&rft.issn=0918-6158&rft.eissn=1347-5215&rft_id=info:doi/10.1248/bpb.32.1283&rft_dat=%3Cproquest_cross%3E20797130%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1449373736&rft_id=info:pmid/19571399&rfr_iscdi=true |