A Proteomic Analysis of Lysosomal Integral Membrane Proteins Reveals the Diverse Composition of the Organelle

Lysosomes are endocytic subcellular compartments that contribute to the degradation and recycling of cellular material. Using highly purified rat liver tritosomes (Triton WR1339-filled lysosomes) and an ion exchange chromatography/LC-tandem MS-based protein/peptide separation and identification proc...

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Veröffentlicht in:	Molecular & cellular proteomics 2005-02, Vol.4 (2), p.133-143
Hauptverfasser:	Bagshaw, Richard D, Mahuran, Don J, Callahan, John W
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Membrane - metabolism Chromatography, Ion Exchange - methods Cytosol - metabolism DNA, Complementary - metabolism Electrophoresis, Polyacrylamide Gel Endoplasmic Reticulum - metabolism Golgi Apparatus - metabolism Immunoblotting Intracellular Membranes - metabolism Liver - metabolism Lysosomes - metabolism Mass Spectrometry Membrane Microdomains - metabolism Microscopy, Electron Protein Transport Proteomics - methods Rats Sepharose - chemistry SNARE Proteins Subcellular Fractions - metabolism Ubiquitin - metabolism Vesicular Transport Proteins - metabolism
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