A guide to hair follicle analysis by transmission electron microscopy: technique and practice

:  Hair follicles contain several tissues and cell types that differentiate down distinct pathways to provide for growth, keratinization and the maintenance of the hair shaft. Electron microscopy is useful for examining the morphological characteristics of developing hair follicles, including specia...

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Veröffentlicht in:Experimental dermatology 2009-07, Vol.18 (7), p.577-582
1. Verfasser: Morioka, Kiyokazu
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description :  Hair follicles contain several tissues and cell types that differentiate down distinct pathways to provide for growth, keratinization and the maintenance of the hair shaft. Electron microscopy is useful for examining the morphological characteristics of developing hair follicles, including special types of keratinization, the timing of keratinization, programmed cell death, cell adhesion and separation, cell movement and changes in organelles. Hair follicles are one of the more challenging targets for electron microscopic analysis, and the use of neonatal animals combined with careful treatment of the samples can yield informative photomicrographs. Detailed protocols and examples of a number of techniques are presented here.
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subjects Animals
Animals, Newborn
Biological and medical sciences
Cell Adhesion
Cell Death
Cell Movement
Dermatology
development
electron microscopy
hair follicle
Hair Follicle - cytology
Hair Follicle - ultrastructure
keratinization
Keratins - ultrastructure
Medical sciences
Microscopy, Electron, Transmission - methods
programmed cell death
Rats
Rats, Sprague-Dawley
title A guide to hair follicle analysis by transmission electron microscopy: technique and practice
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