Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT)
Detection of human immunodeficiency virus (HIV) infections has been enhanced by incorporating p24 antigen detection with current HIV antibody detection using enzyme immunoassays (EIAs). However, screening for HIV antibodies has increased through the use of rapid, lateral-flow HIV antibody detection...
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| Veröffentlicht in: | Journal of virological methods 2009-09, Vol.160 (1), p.14-21 |
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| container_title | Journal of virological methods |
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| creator | Workman, Shon Wells, Susan K. Pau, Chou-Pong Owen, S. Michele Dong, X. Fan LaBorde, Ron Granade, Timothy C. |
| description | Detection of human immunodeficiency virus (HIV) infections has been enhanced by incorporating p24 antigen detection with current HIV antibody detection using enzyme immunoassays (EIAs). However, screening for HIV antibodies has increased through the use of rapid, lateral-flow HIV antibody detection assays that currently do not have the capability to detect HIV p24 antigen. In this report, a lateral-flow based assay using super-paramagnetic particles as the detection marker was developed for the detection of HIV-1 p24 antigen. This magnetic immuno-chromatographic test (MICT) uses an inexpensive, low-maintenance instrument that detects the magnetic moment of the super-paramagnetic particles in a magnetic field. MICT is simple to perform, provides a numerical output for easier determination of reactive results and can be completed in 40
min. The lower limit of detection for HIV-1 p24 spiked into assay sample buffer and 50% plasma was 30
pg/ml for both. Detection of HIV-1 p24 antigen at 50
pg/ml was reproducible in both inter-run and intra-run assays with coefficients of variation of |
| doi_str_mv | 10.1016/j.jviromet.2009.04.003 |
| format | Article |
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min. The lower limit of detection for HIV-1 p24 spiked into assay sample buffer and 50% plasma was 30
pg/ml for both. Detection of HIV-1 p24 antigen at 50
pg/ml was reproducible in both inter-run and intra-run assays with coefficients of variation of <13%. Furthermore, the MICT p24 assay was able to detect intact virus spiked into 50% plasma (lower detection limit of ∼250,000 viral RNA copies/ml). MICT detection of increasing HIV-1 p24 levels in commercially available seroconversion panels by MICT was only slightly later than that detected by much more complex EIAs. MICT could provide a simple, low-cost, and portable method for rapid HIV-1 p24 detection in a variety of testing environments.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/j.jviromet.2009.04.003</identifier><identifier>PMID: 19482361</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Animals ; Antigens, Viral - blood ; Biological and medical sciences ; Chromatography - methods ; Fundamental and applied biological sciences. Psychology ; HIV Core Protein p24 - blood ; HIV Infections - diagnosis ; Human immunodeficiency virus ; Human immunodeficiency virus 1 ; Humans ; Immunomagnetic Separation - methods ; Lateral flow immunoassays ; Mice ; Mice, Inbred BALB C ; Microbiology ; p24 antigen ; Plasma - virology ; Rapid tests ; Reproducibility of Results ; Sensitivity and Specificity ; Techniques used in virology ; Virology</subject><ispartof>Journal of virological methods, 2009-09, Vol.160 (1), p.14-21</ispartof><rights>2009</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c493t-aeb12dc77640c731db62adbb18daf34865ccb96d0876966deb9706c00b4a088f3</citedby><cites>FETCH-LOGICAL-c493t-aeb12dc77640c731db62adbb18daf34865ccb96d0876966deb9706c00b4a088f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jviromet.2009.04.003$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21699289$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19482361$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Workman, Shon</creatorcontrib><creatorcontrib>Wells, Susan K.</creatorcontrib><creatorcontrib>Pau, Chou-Pong</creatorcontrib><creatorcontrib>Owen, S. Michele</creatorcontrib><creatorcontrib>Dong, X. Fan</creatorcontrib><creatorcontrib>LaBorde, Ron</creatorcontrib><creatorcontrib>Granade, Timothy C.</creatorcontrib><title>Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT)</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>Detection of human immunodeficiency virus (HIV) infections has been enhanced by incorporating p24 antigen detection with current HIV antibody detection using enzyme immunoassays (EIAs). However, screening for HIV antibodies has increased through the use of rapid, lateral-flow HIV antibody detection assays that currently do not have the capability to detect HIV p24 antigen. In this report, a lateral-flow based assay using super-paramagnetic particles as the detection marker was developed for the detection of HIV-1 p24 antigen. This magnetic immuno-chromatographic test (MICT) uses an inexpensive, low-maintenance instrument that detects the magnetic moment of the super-paramagnetic particles in a magnetic field. MICT is simple to perform, provides a numerical output for easier determination of reactive results and can be completed in 40
min. The lower limit of detection for HIV-1 p24 spiked into assay sample buffer and 50% plasma was 30
pg/ml for both. Detection of HIV-1 p24 antigen at 50
pg/ml was reproducible in both inter-run and intra-run assays with coefficients of variation of <13%. Furthermore, the MICT p24 assay was able to detect intact virus spiked into 50% plasma (lower detection limit of ∼250,000 viral RNA copies/ml). MICT detection of increasing HIV-1 p24 levels in commercially available seroconversion panels by MICT was only slightly later than that detected by much more complex EIAs. MICT could provide a simple, low-cost, and portable method for rapid HIV-1 p24 detection in a variety of testing environments.</description><subject>Animals</subject><subject>Antigens, Viral - blood</subject><subject>Biological and medical sciences</subject><subject>Chromatography - methods</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HIV Core Protein p24 - blood</subject><subject>HIV Infections - diagnosis</subject><subject>Human immunodeficiency virus</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Immunomagnetic Separation - methods</subject><subject>Lateral flow immunoassays</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Microbiology</subject><subject>p24 antigen</subject><subject>Plasma - virology</subject><subject>Rapid tests</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Techniques used in virology</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v1DAQhi0EotvCX6h8AdFDwvhj7fgGWgFdqQiEClfLsZ2tVxs72Eml_ntc7QLHnmYOzzszegahSwItASLe79v9fchp9HNLAVQLvAVgz9CKdFI1oDr-HK0qKGrP-Bk6L2UPAGvJ2Et0RhTvKBNkhb7_MFNw2PnZ2zmkiNOAr7e_GoInyrGJc9j5iJcS4g6PZhf9HCwO47jE1Ni7eoCZ0y6b6e4Bv_u63dxevUIvBnMo_vWpXqCfnz_dbq6bm29ftpuPN43lis2N8T2hzkopOFjJiOsFNa7vSefMwHgn1tb2SjjopFBCON8rCcIC9NxA1w3sAr09zp1y-r34MusxFOsPBxN9WooWkjPCBH0SpCAkVXxdQXEEbU6lZD_oKYfR5AdNQD9K13v9V7p-lK6B6yq9Bi9PG5Z-9O5_7GS5Am9OgCnWHIZsog3lH0eJUIp2qnIfjpyv4u6Dz7rY4KP1LuT6Hu1SeOqWP7ggomA</recordid><startdate>20090901</startdate><enddate>20090901</enddate><creator>Workman, Shon</creator><creator>Wells, Susan K.</creator><creator>Pau, Chou-Pong</creator><creator>Owen, S. Michele</creator><creator>Dong, X. Fan</creator><creator>LaBorde, Ron</creator><creator>Granade, Timothy C.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20090901</creationdate><title>Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT)</title><author>Workman, Shon ; Wells, Susan K. ; Pau, Chou-Pong ; Owen, S. Michele ; Dong, X. Fan ; LaBorde, Ron ; Granade, Timothy C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c493t-aeb12dc77640c731db62adbb18daf34865ccb96d0876966deb9706c00b4a088f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Antigens, Viral - blood</topic><topic>Biological and medical sciences</topic><topic>Chromatography - methods</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HIV Core Protein p24 - blood</topic><topic>HIV Infections - diagnosis</topic><topic>Human immunodeficiency virus</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Immunomagnetic Separation - methods</topic><topic>Lateral flow immunoassays</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Microbiology</topic><topic>p24 antigen</topic><topic>Plasma - virology</topic><topic>Rapid tests</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Techniques used in virology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Workman, Shon</creatorcontrib><creatorcontrib>Wells, Susan K.</creatorcontrib><creatorcontrib>Pau, Chou-Pong</creatorcontrib><creatorcontrib>Owen, S. Michele</creatorcontrib><creatorcontrib>Dong, X. Fan</creatorcontrib><creatorcontrib>LaBorde, Ron</creatorcontrib><creatorcontrib>Granade, Timothy C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of virological methods</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Workman, Shon</au><au>Wells, Susan K.</au><au>Pau, Chou-Pong</au><au>Owen, S. Michele</au><au>Dong, X. Fan</au><au>LaBorde, Ron</au><au>Granade, Timothy C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT)</atitle><jtitle>Journal of virological methods</jtitle><addtitle>J Virol Methods</addtitle><date>2009-09-01</date><risdate>2009</risdate><volume>160</volume><issue>1</issue><spage>14</spage><epage>21</epage><pages>14-21</pages><issn>0166-0934</issn><eissn>1879-0984</eissn><coden>JVMEDH</coden><abstract>Detection of human immunodeficiency virus (HIV) infections has been enhanced by incorporating p24 antigen detection with current HIV antibody detection using enzyme immunoassays (EIAs). However, screening for HIV antibodies has increased through the use of rapid, lateral-flow HIV antibody detection assays that currently do not have the capability to detect HIV p24 antigen. In this report, a lateral-flow based assay using super-paramagnetic particles as the detection marker was developed for the detection of HIV-1 p24 antigen. This magnetic immuno-chromatographic test (MICT) uses an inexpensive, low-maintenance instrument that detects the magnetic moment of the super-paramagnetic particles in a magnetic field. MICT is simple to perform, provides a numerical output for easier determination of reactive results and can be completed in 40
min. The lower limit of detection for HIV-1 p24 spiked into assay sample buffer and 50% plasma was 30
pg/ml for both. Detection of HIV-1 p24 antigen at 50
pg/ml was reproducible in both inter-run and intra-run assays with coefficients of variation of <13%. Furthermore, the MICT p24 assay was able to detect intact virus spiked into 50% plasma (lower detection limit of ∼250,000 viral RNA copies/ml). MICT detection of increasing HIV-1 p24 levels in commercially available seroconversion panels by MICT was only slightly later than that detected by much more complex EIAs. MICT could provide a simple, low-cost, and portable method for rapid HIV-1 p24 detection in a variety of testing environments.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>19482361</pmid><doi>10.1016/j.jviromet.2009.04.003</doi><tpages>8</tpages></addata></record> |
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| subjects | Animals Antigens, Viral - blood Biological and medical sciences Chromatography - methods Fundamental and applied biological sciences. Psychology HIV Core Protein p24 - blood HIV Infections - diagnosis Human immunodeficiency virus Human immunodeficiency virus 1 Humans Immunomagnetic Separation - methods Lateral flow immunoassays Mice Mice, Inbred BALB C Microbiology p24 antigen Plasma - virology Rapid tests Reproducibility of Results Sensitivity and Specificity Techniques used in virology Virology |
| title | Rapid detection of HIV-1 p24 antigen using magnetic immuno-chromatography (MICT) |
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