Protein recognition onto silica particles using chitosan as intermedium substrate
A novel molecular imprinting method was used to prepare twice‐coated silica particles with specific recognition sites for hemoglobin. Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites fo...
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| Veröffentlicht in: | Journal of biomedical materials research. Part A 2009-08, Vol.90A (2), p.326-332 |
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| container_end_page | 332 |
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| container_issue | 2 |
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| container_title | Journal of biomedical materials research. Part A |
| container_volume | 90A |
| creator | Xia, Yong-Qing Guo, Tian-Ying Zhao, Hai-Li Song, Mou-Dao Zhang, Bang-Hua Zhang, Bao-Long |
| description | A novel molecular imprinting method was used to prepare twice‐coated silica particles with specific recognition sites for hemoglobin. Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites for introducing aldehyde groups. After hemoglobin was covalently immobilized by forming imine bonds with the aldehyde groups, acrylamide was then polymerized onto chitosan‐coated silica particles to form the recognition sites. The obtained hemoglobin imprinted [molecularly imprinted polymer (MIP)] beads were characterized by FTIR spectroscopy, thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The MIP particles exhibited selectively adsorption for the imprinted protein compared to the nonselectively adsorption for most of proteins of the nonimprinted (NIP) beads. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009 |
| doi_str_mv | 10.1002/jbm.a.32084 |
| format | Article |
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Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites for introducing aldehyde groups. After hemoglobin was covalently immobilized by forming imine bonds with the aldehyde groups, acrylamide was then polymerized onto chitosan‐coated silica particles to form the recognition sites. The obtained hemoglobin imprinted [molecularly imprinted polymer (MIP)] beads were characterized by FTIR spectroscopy, thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The MIP particles exhibited selectively adsorption for the imprinted protein compared to the nonselectively adsorption for most of proteins of the nonimprinted (NIP) beads. © 2008 Wiley Periodicals, Inc. 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Part A</title><addtitle>J. Biomed. Mater. Res</addtitle><description>A novel molecular imprinting method was used to prepare twice‐coated silica particles with specific recognition sites for hemoglobin. Chitosan was used as an intermedium to be coated on silica particles via phase inversion process, and the abundance of exposed amine groups (NH2) were active sites for introducing aldehyde groups. After hemoglobin was covalently immobilized by forming imine bonds with the aldehyde groups, acrylamide was then polymerized onto chitosan‐coated silica particles to form the recognition sites. The obtained hemoglobin imprinted [molecularly imprinted polymer (MIP)] beads were characterized by FTIR spectroscopy, thermogravimetric analysis (TG), and scanning electron microscopy (SEM). The MIP particles exhibited selectively adsorption for the imprinted protein compared to the nonselectively adsorption for most of proteins of the nonimprinted (NIP) beads. © 2008 Wiley Periodicals, Inc. J Biomed Mater Res, 2009</description><subject>Acrylamides - chemistry</subject><subject>Adsorption</subject><subject>Aldehydes - chemistry</subject><subject>Amines - chemistry</subject><subject>Animals</subject><subject>Biocompatible Materials - chemistry</subject><subject>Catalytic Domain</subject><subject>Cattle</subject><subject>chitosan</subject><subject>Chitosan - chemistry</subject><subject>Hemoglobins - chemistry</subject><subject>Microscopy, Electron, Scanning</subject><subject>molecular imprinting</subject><subject>Polymers - chemistry</subject><subject>protein recognition</subject><subject>silica particles</subject><subject>Silicon Dioxide - chemistry</subject><subject>Spectroscopy, Fourier Transform Infrared</subject><subject>Thermogravimetry - methods</subject><issn>1549-3296</issn><issn>1552-4965</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUtP3DAURq2qqLy66h551U2Vwa_EzhIoDKDhUakVVTeW47kB0yQebEfAv8cwU9iV1b2L8x3p3g-hL5RMKCFs97bpJ2bCGVHiA9qgZckKUVflx-dd1AVndbWONmO8zXBFSvYJrVNVEsW52kA_LoNP4AYcwPrrwSXnB-yH5HF0nbMGL0xIznYQ8RjdcI3tjUs-mgGbiN2QIPQwd2OP49jEFEyCbbTWmi7C59XcQr-ODn8eHBezi-nJwd6ssILVojBkbmzbkFLwdk4Z56YVrZSSqjkAoU0lhW24hdZIqA2hLbNWKSUbqKCGpuJb6OvSuwj-boSYdO-iha4zA_gx6izghFb1uyAXNakkV--CjJKskyyD35agDT7GAK1eBNeb8Kgp0c-d6NyJNvqlk0zvrLRjk5_1xq5KyABdAveug8f_ufTp_tk_abHMuJjg4TVjwt98OJelvjqf6t-z_T_l0fepvuJPOrmoJQ</recordid><startdate>200908</startdate><enddate>200908</enddate><creator>Xia, Yong-Qing</creator><creator>Guo, Tian-Ying</creator><creator>Zhao, Hai-Li</creator><creator>Song, Mou-Dao</creator><creator>Zhang, Bang-Hua</creator><creator>Zhang, Bao-Long</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7SR</scope><scope>7TB</scope><scope>7U5</scope><scope>8BQ</scope><scope>F28</scope><scope>JG9</scope><scope>L7M</scope><scope>7X8</scope></search><sort><creationdate>200908</creationdate><title>Protein recognition onto silica particles using chitosan as intermedium substrate</title><author>Xia, Yong-Qing ; Guo, Tian-Ying ; Zhao, Hai-Li ; Song, Mou-Dao ; Zhang, Bang-Hua ; Zhang, Bao-Long</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4294-a0dacfb0543fd1233af4f77718dee01b674cb3cefa7e9a01f2cc8887be6e9eb63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Acrylamides - chemistry</topic><topic>Adsorption</topic><topic>Aldehydes - chemistry</topic><topic>Amines - chemistry</topic><topic>Animals</topic><topic>Biocompatible Materials - chemistry</topic><topic>Catalytic Domain</topic><topic>Cattle</topic><topic>chitosan</topic><topic>Chitosan - chemistry</topic><topic>Hemoglobins - chemistry</topic><topic>Microscopy, Electron, Scanning</topic><topic>molecular imprinting</topic><topic>Polymers - chemistry</topic><topic>protein recognition</topic><topic>silica particles</topic><topic>Silicon Dioxide - chemistry</topic><topic>Spectroscopy, Fourier Transform Infrared</topic><topic>Thermogravimetry - methods</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Xia, Yong-Qing</creatorcontrib><creatorcontrib>Guo, Tian-Ying</creatorcontrib><creatorcontrib>Zhao, Hai-Li</creatorcontrib><creatorcontrib>Song, Mou-Dao</creatorcontrib><creatorcontrib>Zhang, Bang-Hua</creatorcontrib><creatorcontrib>Zhang, Bao-Long</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>METADEX</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Materials Research Database</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of biomedical materials research. 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| subjects | Acrylamides - chemistry Adsorption Aldehydes - chemistry Amines - chemistry Animals Biocompatible Materials - chemistry Catalytic Domain Cattle chitosan Chitosan - chemistry Hemoglobins - chemistry Microscopy, Electron, Scanning molecular imprinting Polymers - chemistry protein recognition silica particles Silicon Dioxide - chemistry Spectroscopy, Fourier Transform Infrared Thermogravimetry - methods |
| title | Protein recognition onto silica particles using chitosan as intermedium substrate |
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