Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns

We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent prot...

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Veröffentlicht in:	Fungal genetics and biology 2005-03, Vol.42 (3), p.191-199
Hauptverfasser:	Burns, C., Gregory, K.E., Kirby, M., Cheung, M.K., Riquelme, M., Elliott, T.J., Challen, M.P., Bailey, A., Foster, G.D.
Format:	Artikel
Sprache:	eng
Schlagworte:	Agaricus - enzymology Agaricus - genetics Agaricus bisporus Base Sequence Cloning, Molecular Coprinus - enzymology Coprinus - genetics Coprinus cinereus DNA Primers DNA, Fungal - genetics DNA, Fungal - isolation & purification Escherichia coli - genetics Gene Expression Regulation, Fungal Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Introns - genetics Plants, Genetically Modified - enzymology Polymerase Chain Reaction Promoter Regions, Genetic - genetics Restriction Mapping
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