Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns
We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes Agaricus bisporus and Coprinus cinereus using green fluorescent prot...
Gespeichert in:
| Veröffentlicht in: | Fungal genetics and biology 2005-03, Vol.42 (3), p.191-199 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 199 |
|---|---|
| container_issue | 3 |
| container_start_page | 191 |
| container_title | Fungal genetics and biology |
| container_volume | 42 |
| creator | Burns, C. Gregory, K.E. Kirby, M. Cheung, M.K. Riquelme, M. Elliott, T.J. Challen, M.P. Bailey, A. Foster, G.D. |
| description | We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes
Agaricus bisporus and
Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the
C. cinereus trp1 promoter and
A. bisporus trp2 and
gpdII promoters proving successful in driving expression in
C. cinereus, with the
gpdII promoter also functioning in
A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in
C. cinereus and
A. bisporus is the presence of an intron. This is the first reported expression of GFP in either
C. cinereus or
A. bisporus. |
| doi_str_mv | 10.1016/j.fgb.2004.11.005 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_67428857</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S108718450400180X</els_id><sourcerecordid>20812964</sourcerecordid><originalsourceid>FETCH-LOGICAL-c479t-4495309b77adee93aa0acc1b5165e3939b6e70b9c9ce1d760dc1051db721616c3</originalsourceid><addsrcrecordid>eNqFkU1r3DAQhkVpadKkP6CX4lNvdmdsfVj0FJYkLQTaQ3MWkjxOtKytjWSX9t9Hyy7k1pw0gmdehvdh7BNCg4Dy67YZH1zTAvAGsQEQb9g5gpY16E69Pcy9qrHn4ox9yHkLgCg4vmdnKBSonsM5c9fjGHygealub35V9HefKOcQ5yrM1fJI1bTmxxTjlKurB5uCX3PlQt7HVAY7D9Um7lOYy8eHmRKVIdHTGkpKSVhSnPMlezfaXaaPp_eC3d9c_958r-9-3v7YXN3Vniu91Jxr0YF2StmBSHfWgvUenUApqNOddpIUOO21JxyUhMEjCBycalGi9N0F-3LM3af4tFJezBSyp93OzhTXbKTibd8L9SrYQo-tlvxVEFW5uUcoIB5Bn2LOiUZTSpls-mcQzEGV2ZqiyhxUGURTVJWdz6fw1U00vGyc3BTg2xGgUtqfQMnkgyhPQ2nXL2aI4T_xz6HTpMI</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>17495810</pqid></control><display><type>article</type><title>Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Burns, C. ; Gregory, K.E. ; Kirby, M. ; Cheung, M.K. ; Riquelme, M. ; Elliott, T.J. ; Challen, M.P. ; Bailey, A. ; Foster, G.D.</creator><creatorcontrib>Burns, C. ; Gregory, K.E. ; Kirby, M. ; Cheung, M.K. ; Riquelme, M. ; Elliott, T.J. ; Challen, M.P. ; Bailey, A. ; Foster, G.D.</creatorcontrib><description>We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes
Agaricus bisporus and
Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the
C. cinereus trp1 promoter and
A. bisporus trp2 and
gpdII promoters proving successful in driving expression in
C. cinereus, with the
gpdII promoter also functioning in
A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in
C. cinereus and
A. bisporus is the presence of an intron. This is the first reported expression of GFP in either
C. cinereus or
A. bisporus.</description><identifier>ISSN: 1087-1845</identifier><identifier>EISSN: 1096-0937</identifier><identifier>DOI: 10.1016/j.fgb.2004.11.005</identifier><identifier>PMID: 15707840</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Agaricus - enzymology ; Agaricus - genetics ; Agaricus bisporus ; Base Sequence ; Cloning, Molecular ; Coprinus - enzymology ; Coprinus - genetics ; Coprinus cinereus ; DNA Primers ; DNA, Fungal - genetics ; DNA, Fungal - isolation & purification ; Escherichia coli - genetics ; Gene Expression Regulation, Fungal ; Genes, Reporter ; Green Fluorescent Proteins - genetics ; Green Fluorescent Proteins - metabolism ; Introns - genetics ; Plants, Genetically Modified - enzymology ; Polymerase Chain Reaction ; Promoter Regions, Genetic - genetics ; Restriction Mapping</subject><ispartof>Fungal genetics and biology, 2005-03, Vol.42 (3), p.191-199</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c479t-4495309b77adee93aa0acc1b5165e3939b6e70b9c9ce1d760dc1051db721616c3</citedby><cites>FETCH-LOGICAL-c479t-4495309b77adee93aa0acc1b5165e3939b6e70b9c9ce1d760dc1051db721616c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S108718450400180X$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27903,27904,65309</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15707840$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Burns, C.</creatorcontrib><creatorcontrib>Gregory, K.E.</creatorcontrib><creatorcontrib>Kirby, M.</creatorcontrib><creatorcontrib>Cheung, M.K.</creatorcontrib><creatorcontrib>Riquelme, M.</creatorcontrib><creatorcontrib>Elliott, T.J.</creatorcontrib><creatorcontrib>Challen, M.P.</creatorcontrib><creatorcontrib>Bailey, A.</creatorcontrib><creatorcontrib>Foster, G.D.</creatorcontrib><title>Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns</title><title>Fungal genetics and biology</title><addtitle>Fungal Genet Biol</addtitle><description>We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes
Agaricus bisporus and
Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the
C. cinereus trp1 promoter and
A. bisporus trp2 and
gpdII promoters proving successful in driving expression in
C. cinereus, with the
gpdII promoter also functioning in
A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in
C. cinereus and
A. bisporus is the presence of an intron. This is the first reported expression of GFP in either
C. cinereus or
A. bisporus.</description><subject>Agaricus - enzymology</subject><subject>Agaricus - genetics</subject><subject>Agaricus bisporus</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>Coprinus - enzymology</subject><subject>Coprinus - genetics</subject><subject>Coprinus cinereus</subject><subject>DNA Primers</subject><subject>DNA, Fungal - genetics</subject><subject>DNA, Fungal - isolation & purification</subject><subject>Escherichia coli - genetics</subject><subject>Gene Expression Regulation, Fungal</subject><subject>Genes, Reporter</subject><subject>Green Fluorescent Proteins - genetics</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Introns - genetics</subject><subject>Plants, Genetically Modified - enzymology</subject><subject>Polymerase Chain Reaction</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>Restriction Mapping</subject><issn>1087-1845</issn><issn>1096-0937</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1r3DAQhkVpadKkP6CX4lNvdmdsfVj0FJYkLQTaQ3MWkjxOtKytjWSX9t9Hyy7k1pw0gmdehvdh7BNCg4Dy67YZH1zTAvAGsQEQb9g5gpY16E69Pcy9qrHn4ox9yHkLgCg4vmdnKBSonsM5c9fjGHygealub35V9HefKOcQ5yrM1fJI1bTmxxTjlKurB5uCX3PlQt7HVAY7D9Um7lOYy8eHmRKVIdHTGkpKSVhSnPMlezfaXaaPp_eC3d9c_958r-9-3v7YXN3Vniu91Jxr0YF2StmBSHfWgvUenUApqNOddpIUOO21JxyUhMEjCBycalGi9N0F-3LM3af4tFJezBSyp93OzhTXbKTibd8L9SrYQo-tlvxVEFW5uUcoIB5Bn2LOiUZTSpls-mcQzEGV2ZqiyhxUGURTVJWdz6fw1U00vGyc3BTg2xGgUtqfQMnkgyhPQ2nXL2aI4T_xz6HTpMI</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Burns, C.</creator><creator>Gregory, K.E.</creator><creator>Kirby, M.</creator><creator>Cheung, M.K.</creator><creator>Riquelme, M.</creator><creator>Elliott, T.J.</creator><creator>Challen, M.P.</creator><creator>Bailey, A.</creator><creator>Foster, G.D.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050301</creationdate><title>Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns</title><author>Burns, C. ; Gregory, K.E. ; Kirby, M. ; Cheung, M.K. ; Riquelme, M. ; Elliott, T.J. ; Challen, M.P. ; Bailey, A. ; Foster, G.D.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c479t-4495309b77adee93aa0acc1b5165e3939b6e70b9c9ce1d760dc1051db721616c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Agaricus - enzymology</topic><topic>Agaricus - genetics</topic><topic>Agaricus bisporus</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>Coprinus - enzymology</topic><topic>Coprinus - genetics</topic><topic>Coprinus cinereus</topic><topic>DNA Primers</topic><topic>DNA, Fungal - genetics</topic><topic>DNA, Fungal - isolation & purification</topic><topic>Escherichia coli - genetics</topic><topic>Gene Expression Regulation, Fungal</topic><topic>Genes, Reporter</topic><topic>Green Fluorescent Proteins - genetics</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Introns - genetics</topic><topic>Plants, Genetically Modified - enzymology</topic><topic>Polymerase Chain Reaction</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>Restriction Mapping</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Burns, C.</creatorcontrib><creatorcontrib>Gregory, K.E.</creatorcontrib><creatorcontrib>Kirby, M.</creatorcontrib><creatorcontrib>Cheung, M.K.</creatorcontrib><creatorcontrib>Riquelme, M.</creatorcontrib><creatorcontrib>Elliott, T.J.</creatorcontrib><creatorcontrib>Challen, M.P.</creatorcontrib><creatorcontrib>Bailey, A.</creatorcontrib><creatorcontrib>Foster, G.D.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Fungal genetics and biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Burns, C.</au><au>Gregory, K.E.</au><au>Kirby, M.</au><au>Cheung, M.K.</au><au>Riquelme, M.</au><au>Elliott, T.J.</au><au>Challen, M.P.</au><au>Bailey, A.</au><au>Foster, G.D.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns</atitle><jtitle>Fungal genetics and biology</jtitle><addtitle>Fungal Genet Biol</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>42</volume><issue>3</issue><spage>191</spage><epage>199</epage><pages>191-199</pages><issn>1087-1845</issn><eissn>1096-0937</eissn><abstract>We have developed a “Molecular Toolkit” comprising interchangeable promoters and marker genes to facilitate transformation of homobasidiomycete mushrooms. We describe the evaluation of a range of promoters in the homobasidiomycetes
Agaricus bisporus and
Coprinus cinereus using green fluorescent protein (GFP) as a reporter gene; the
C. cinereus trp1 promoter and
A. bisporus trp2 and
gpdII promoters proving successful in driving expression in
C. cinereus, with the
gpdII promoter also functioning in
A. bisporus. Our investigations demonstrate that a prerequisite for GFP expression in
C. cinereus and
A. bisporus is the presence of an intron. This is the first reported expression of GFP in either
C. cinereus or
A. bisporus.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15707840</pmid><doi>10.1016/j.fgb.2004.11.005</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1087-1845 |
| ispartof | Fungal genetics and biology, 2005-03, Vol.42 (3), p.191-199 |
| issn | 1087-1845 1096-0937 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67428857 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Agaricus - enzymology Agaricus - genetics Agaricus bisporus Base Sequence Cloning, Molecular Coprinus - enzymology Coprinus - genetics Coprinus cinereus DNA Primers DNA, Fungal - genetics DNA, Fungal - isolation & purification Escherichia coli - genetics Gene Expression Regulation, Fungal Genes, Reporter Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Introns - genetics Plants, Genetically Modified - enzymology Polymerase Chain Reaction Promoter Regions, Genetic - genetics Restriction Mapping |
| title | Efficient GFP expression in the mushrooms Agaricus bisporus and Coprinus cinereus requires introns |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-27T23%3A54%3A27IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Efficient%20GFP%20expression%20in%20the%20mushrooms%20Agaricus%20bisporus%20and%20Coprinus%20cinereus%20requires%20introns&rft.jtitle=Fungal%20genetics%20and%20biology&rft.au=Burns,%20C.&rft.date=2005-03-01&rft.volume=42&rft.issue=3&rft.spage=191&rft.epage=199&rft.pages=191-199&rft.issn=1087-1845&rft.eissn=1096-0937&rft_id=info:doi/10.1016/j.fgb.2004.11.005&rft_dat=%3Cproquest_cross%3E20812964%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=17495810&rft_id=info:pmid/15707840&rft_els_id=S108718450400180X&rfr_iscdi=true |