Citri Reticulatae Viride Pericarpium extract induced apoptosis in SNU-C4, human colon cancer cells
Citri Reticulatae Viride Pericarpium (CR) has been used traditionally in Korea to promote the Liver Qi activity and the function of digestive system. We investigated whether the immature peels of Citrus reticulata Blanco (Rutaceae) induced cell-death on SNU-C4, human colon cancer cells. Cytotoxicity...
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| Veröffentlicht in: | Journal of ethnopharmacology 2005-02, Vol.97 (2), p.231-235 |
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| creator | Kang, Soon Ah Park, Hae Jeong Kim, Mi-Ja Lee, Se-Yun Han, Sang-Won Leem, Kang-Hyun |
| description | Citri Reticulatae Viride Pericarpium (CR) has been used traditionally in Korea to promote the Liver Qi activity and the function of digestive system. We investigated whether the immature peels of
Citrus reticulata Blanco (Rutaceae) induced cell-death on SNU-C4, human colon cancer cells. Cytotoxicity of CR was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cell death was identified as apoptosis using 4,6-diamidineo-2-phenylindole (DAPI) staining and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of pro-apoptotic gene,
Bax, was increased and the expression of anti-apoptotic gene,
Bcl-2, was decreased by CR-treatment. The expression and activity of major apoptotic gene,
caspase-3 was significantly increased by CR-treatment. Considering the above results, CR could induce the apoptosis on SNU-C4, human colon cancer cells via Bax-related caspase-3 activation. And it might provide the experimental data for the future clinical use of CR on colon cancer. |
| doi_str_mv | 10.1016/j.jep.2004.11.012 |
| format | Article |
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Citrus reticulata Blanco (Rutaceae) induced cell-death on SNU-C4, human colon cancer cells. Cytotoxicity of CR was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cell death was identified as apoptosis using 4,6-diamidineo-2-phenylindole (DAPI) staining and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of pro-apoptotic gene,
Bax, was increased and the expression of anti-apoptotic gene,
Bcl-2, was decreased by CR-treatment. The expression and activity of major apoptotic gene,
caspase-3 was significantly increased by CR-treatment. Considering the above results, CR could induce the apoptosis on SNU-C4, human colon cancer cells via Bax-related caspase-3 activation. And it might provide the experimental data for the future clinical use of CR on colon cancer.</description><identifier>ISSN: 0378-8741</identifier><identifier>EISSN: 1872-7573</identifier><identifier>DOI: 10.1016/j.jep.2004.11.012</identifier><identifier>PMID: 15707758</identifier><identifier>CODEN: JOETD7</identifier><language>eng</language><publisher>Shannon: Elsevier Ireland Ltd</publisher><subject>Anti-tumor activity ; anticarcinogenic activity ; Antineoplastic Agents - isolation & purification ; Antineoplastic Agents - therapeutic use ; Apoptosis ; Apoptosis - drug effects ; Biological and medical sciences ; Caspase 3 ; Caspases - metabolism ; cell lines ; Cell Survival - drug effects ; Citrus ; citrus peels ; Citrus reticulata ; Colon cancer ; Colonic Neoplasms - drug therapy ; Colonic Neoplasms - enzymology ; colorectal neoplasms ; cytotoxicity ; Enzyme Activation - drug effects ; General pharmacology ; Humans ; In Situ Nick-End Labeling ; Korean traditional medicine ; Medical sciences ; medicinal plants ; Oriental traditional medicine ; Pharmacognosy. Homeopathy. Health food ; Pharmacology. Drug treatments ; Phytotherapy ; plant extracts ; Plant Preparations - therapeutic use ; Reverse Transcriptase Polymerase Chain Reaction ; SNU-C4 ; Tumor Cells, Cultured</subject><ispartof>Journal of ethnopharmacology, 2005-02, Vol.97 (2), p.231-235</ispartof><rights>2004 Elsevier Ireland Ltd</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c405t-1626bb291c2ab63ef417879168ab5c204fed17885abd8d42f5577c89515043153</citedby><cites>FETCH-LOGICAL-c405t-1626bb291c2ab63ef417879168ab5c204fed17885abd8d42f5577c89515043153</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jep.2004.11.012$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16534659$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15707758$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kang, Soon Ah</creatorcontrib><creatorcontrib>Park, Hae Jeong</creatorcontrib><creatorcontrib>Kim, Mi-Ja</creatorcontrib><creatorcontrib>Lee, Se-Yun</creatorcontrib><creatorcontrib>Han, Sang-Won</creatorcontrib><creatorcontrib>Leem, Kang-Hyun</creatorcontrib><title>Citri Reticulatae Viride Pericarpium extract induced apoptosis in SNU-C4, human colon cancer cells</title><title>Journal of ethnopharmacology</title><addtitle>J Ethnopharmacol</addtitle><description>Citri Reticulatae Viride Pericarpium (CR) has been used traditionally in Korea to promote the Liver Qi activity and the function of digestive system. We investigated whether the immature peels of
Citrus reticulata Blanco (Rutaceae) induced cell-death on SNU-C4, human colon cancer cells. Cytotoxicity of CR was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cell death was identified as apoptosis using 4,6-diamidineo-2-phenylindole (DAPI) staining and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of pro-apoptotic gene,
Bax, was increased and the expression of anti-apoptotic gene,
Bcl-2, was decreased by CR-treatment. The expression and activity of major apoptotic gene,
caspase-3 was significantly increased by CR-treatment. Considering the above results, CR could induce the apoptosis on SNU-C4, human colon cancer cells via Bax-related caspase-3 activation. And it might provide the experimental data for the future clinical use of CR on colon cancer.</description><subject>Anti-tumor activity</subject><subject>anticarcinogenic activity</subject><subject>Antineoplastic Agents - isolation & purification</subject><subject>Antineoplastic Agents - therapeutic use</subject><subject>Apoptosis</subject><subject>Apoptosis - drug effects</subject><subject>Biological and medical sciences</subject><subject>Caspase 3</subject><subject>Caspases - metabolism</subject><subject>cell lines</subject><subject>Cell Survival - drug effects</subject><subject>Citrus</subject><subject>citrus peels</subject><subject>Citrus reticulata</subject><subject>Colon cancer</subject><subject>Colonic Neoplasms - drug therapy</subject><subject>Colonic Neoplasms - enzymology</subject><subject>colorectal neoplasms</subject><subject>cytotoxicity</subject><subject>Enzyme Activation - drug effects</subject><subject>General pharmacology</subject><subject>Humans</subject><subject>In Situ Nick-End Labeling</subject><subject>Korean traditional medicine</subject><subject>Medical sciences</subject><subject>medicinal plants</subject><subject>Oriental traditional medicine</subject><subject>Pharmacognosy. Homeopathy. Health food</subject><subject>Pharmacology. Drug treatments</subject><subject>Phytotherapy</subject><subject>plant extracts</subject><subject>Plant Preparations - therapeutic use</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>SNU-C4</subject><subject>Tumor Cells, Cultured</subject><issn>0378-8741</issn><issn>1872-7573</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kE1v1DAQhi0EotvCD-ACvsCJhBnHjr3qCa34qFRRRFmuluNMwKtkk9pJBf8er3al3nqZkV49M3r1MPYKoUTA-sOu3NFUCgBZIpaA4glbodGi0EpXT9kKKm0KoyWesfOUdgCgUcJzdoZKg9bKrFizCXMM_AfNwS-9mx3xXyGGlvh3isG7OIVl4PR3js7PPOzbxVPL3TRO85hCygm__bYtNvI9_7MMbs_92I95ur2nyD31fXrBnnWuT_TytC_Y9vOnn5uvxfXNl6vNx-vCS1BzgbWom0as0QvX1BV1ErXRa6yNa5QXIDtqc2KUa1rTStEppbU3a4UKZIWqumDvjn-nON4tlGY7hHRo4PY0LsnWWgqJQmQQj6CPY0qROjvFMLj4zyLYg1i7s1msPYi1iDaLzTevT8-XZqD24eJkMgNvT4BL3vVdzAZCeuBqVclarTP35sh1brTud8zM9lYAVoAARhqdicsjQVnWfaBokw-UfbYhkp9tO4ZHiv4HVSadwg</recordid><startdate>20050228</startdate><enddate>20050228</enddate><creator>Kang, Soon Ah</creator><creator>Park, Hae Jeong</creator><creator>Kim, Mi-Ja</creator><creator>Lee, Se-Yun</creator><creator>Han, Sang-Won</creator><creator>Leem, Kang-Hyun</creator><general>Elsevier Ireland Ltd</general><general>Elsevier</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050228</creationdate><title>Citri Reticulatae Viride Pericarpium extract induced apoptosis in SNU-C4, human colon cancer cells</title><author>Kang, Soon Ah ; Park, Hae Jeong ; Kim, Mi-Ja ; Lee, Se-Yun ; Han, Sang-Won ; Leem, Kang-Hyun</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c405t-1626bb291c2ab63ef417879168ab5c204fed17885abd8d42f5577c89515043153</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Anti-tumor activity</topic><topic>anticarcinogenic activity</topic><topic>Antineoplastic Agents - isolation & purification</topic><topic>Antineoplastic Agents - therapeutic use</topic><topic>Apoptosis</topic><topic>Apoptosis - drug effects</topic><topic>Biological and medical sciences</topic><topic>Caspase 3</topic><topic>Caspases - metabolism</topic><topic>cell lines</topic><topic>Cell Survival - drug effects</topic><topic>Citrus</topic><topic>citrus peels</topic><topic>Citrus reticulata</topic><topic>Colon cancer</topic><topic>Colonic Neoplasms - drug therapy</topic><topic>Colonic Neoplasms - enzymology</topic><topic>colorectal neoplasms</topic><topic>cytotoxicity</topic><topic>Enzyme Activation - drug effects</topic><topic>General pharmacology</topic><topic>Humans</topic><topic>In Situ Nick-End Labeling</topic><topic>Korean traditional medicine</topic><topic>Medical sciences</topic><topic>medicinal plants</topic><topic>Oriental traditional medicine</topic><topic>Pharmacognosy. Homeopathy. Health food</topic><topic>Pharmacology. Drug treatments</topic><topic>Phytotherapy</topic><topic>plant extracts</topic><topic>Plant Preparations - therapeutic use</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>SNU-C4</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kang, Soon Ah</creatorcontrib><creatorcontrib>Park, Hae Jeong</creatorcontrib><creatorcontrib>Kim, Mi-Ja</creatorcontrib><creatorcontrib>Lee, Se-Yun</creatorcontrib><creatorcontrib>Han, Sang-Won</creatorcontrib><creatorcontrib>Leem, Kang-Hyun</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of ethnopharmacology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kang, Soon Ah</au><au>Park, Hae Jeong</au><au>Kim, Mi-Ja</au><au>Lee, Se-Yun</au><au>Han, Sang-Won</au><au>Leem, Kang-Hyun</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Citri Reticulatae Viride Pericarpium extract induced apoptosis in SNU-C4, human colon cancer cells</atitle><jtitle>Journal of ethnopharmacology</jtitle><addtitle>J Ethnopharmacol</addtitle><date>2005-02-28</date><risdate>2005</risdate><volume>97</volume><issue>2</issue><spage>231</spage><epage>235</epage><pages>231-235</pages><issn>0378-8741</issn><eissn>1872-7573</eissn><coden>JOETD7</coden><abstract>Citri Reticulatae Viride Pericarpium (CR) has been used traditionally in Korea to promote the Liver Qi activity and the function of digestive system. We investigated whether the immature peels of
Citrus reticulata Blanco (Rutaceae) induced cell-death on SNU-C4, human colon cancer cells. Cytotoxicity of CR was assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay. The cell death was identified as apoptosis using 4,6-diamidineo-2-phenylindole (DAPI) staining and terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay. The expression of pro-apoptotic gene,
Bax, was increased and the expression of anti-apoptotic gene,
Bcl-2, was decreased by CR-treatment. The expression and activity of major apoptotic gene,
caspase-3 was significantly increased by CR-treatment. Considering the above results, CR could induce the apoptosis on SNU-C4, human colon cancer cells via Bax-related caspase-3 activation. And it might provide the experimental data for the future clinical use of CR on colon cancer.</abstract><cop>Shannon</cop><pub>Elsevier Ireland Ltd</pub><pmid>15707758</pmid><doi>10.1016/j.jep.2004.11.012</doi><tpages>5</tpages></addata></record> |
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| subjects | Anti-tumor activity anticarcinogenic activity Antineoplastic Agents - isolation & purification Antineoplastic Agents - therapeutic use Apoptosis Apoptosis - drug effects Biological and medical sciences Caspase 3 Caspases - metabolism cell lines Cell Survival - drug effects Citrus citrus peels Citrus reticulata Colon cancer Colonic Neoplasms - drug therapy Colonic Neoplasms - enzymology colorectal neoplasms cytotoxicity Enzyme Activation - drug effects General pharmacology Humans In Situ Nick-End Labeling Korean traditional medicine Medical sciences medicinal plants Oriental traditional medicine Pharmacognosy. Homeopathy. Health food Pharmacology. Drug treatments Phytotherapy plant extracts Plant Preparations - therapeutic use Reverse Transcriptase Polymerase Chain Reaction SNU-C4 Tumor Cells, Cultured |
| title | Citri Reticulatae Viride Pericarpium extract induced apoptosis in SNU-C4, human colon cancer cells |
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