Interaction of DNA Fragmentation Factor (DFF) with DNA Reveals an Unprecedented Mechanism for Nuclease Inhibition and Suggests That DFF Can Be Activated in a DNA-bound State

DNA fragmentation factor (DFF) is a complex of the DNase DFF40 (CAD) and its chaperone/inhibitor DFF45 (ICAD-L) that can be activated during apoptosis to induce DNA fragmentation. Here, we demonstrate that DFF directly binds to DNA in vitro without promoting DNA cleavage. DNA binding by DFF is media...

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Veröffentlicht in:	The Journal of biological chemistry 2005-02, Vol.280 (7), p.6005-6015
Hauptverfasser:	Korn, Christian, Scholz, Sebastian R., Gimadutdinow, Oleg, Lurz, Rudi, Pingoud, Alfred, Meiss, Gregor
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Apoptosis Apoptosis Regulatory Proteins Base Sequence Caspase 3 Caspases - metabolism Chromatin - metabolism Deoxyribonucleases - antagonists & inhibitors Deoxyribonucleases - chemistry Deoxyribonucleases - genetics Deoxyribonucleases - metabolism DNA - genetics DNA - metabolism DNA - ultrastructure Enzyme Activation HeLa Cells Humans Mice Microscopy, Electron, Transmission Models, Biological Models, Molecular Poly-ADP-Ribose Binding Proteins Protein Binding Protein Conformation Protein Subunits - antagonists & inhibitors Protein Subunits - chemistry Protein Subunits - genetics Protein Subunits - metabolism Protein Transport Proteins - genetics Proteins - metabolism Recombinant Proteins - chemistry Recombinant Proteins - genetics Recombinant Proteins - metabolism Substrate Specificity Transfection
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