Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells
To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐α (TNF‐α), and interleukin‐1α (IL‐1α) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs)...
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| Veröffentlicht in: | Cell biochemistry and function 2009-07, Vol.27 (5), p.284-288 |
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| creator | Hu, Yiyi Chen, Xi Duan, Huiqin Hu, Yuanliang Mu, Xiang |
| description | To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐α (TNF‐α), and interleukin‐1α (IL‐1α) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml−1 for 3 h and then treated with PD at 1, 5, and 10 mg ml−1 and its seven ingredients at 1, 5, and 10 µg ml−1 for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B4, berberine, jatrorrhizine, and aesculin downregulated TNF‐α expression; PD, anemoside B4, berberine, and palmatine decreased the content of IL‐1α. It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐α, and IL‐1α in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd. |
| doi_str_mv | 10.1002/cbf.1570 |
| format | Article |
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RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml−1 for 3 h and then treated with PD at 1, 5, and 10 mg ml−1 and its seven ingredients at 1, 5, and 10 µg ml−1 for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B4, berberine, jatrorrhizine, and aesculin downregulated TNF‐α expression; PD, anemoside B4, berberine, and palmatine decreased the content of IL‐1α. It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐α, and IL‐1α in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.1570</identifier><identifier>PMID: 19472295</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Animals ; Cells ; Cytokines - metabolism ; Drugs, Chinese Herbal - chemistry ; Drugs, Chinese Herbal - pharmacology ; Endothelial Cells - drug effects ; Endothelial Cells - metabolism ; Endothelin-1 - metabolism ; Endothelium, Vascular - cytology ; Endothelium, Vascular - metabolism ; ET-1 ; IL-1α ; Inflammation Mediators - metabolism ; Interleukin-1alpha - metabolism ; Intestines - blood supply ; Lipopolysaccharides - pharmacology ; LPS ; Nitric Oxide - biosynthesis ; Nitric Oxide - metabolism ; Plant Preparations - chemistry ; Plant Preparations - pharmacology ; Pulsatilla ; Pulsatilla - chemistry ; Rats ; RIMECs ; TNF-α ; Tumor Necrosis Factor-alpha - metabolism</subject><ispartof>Cell biochemistry and function, 2009-07, Vol.27 (5), p.284-288</ispartof><rights>Copyright © 2009 John Wiley & Sons, Ltd.</rights><rights>(c) 2009 John Wiley & Sons, Ltd.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3880-5151f50922b5563eb5223314da466706a339e70985949e44b89b70c375b72de93</citedby><cites>FETCH-LOGICAL-c3880-5151f50922b5563eb5223314da466706a339e70985949e44b89b70c375b72de93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.1570$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.1570$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,777,781,1412,27905,27906,45555,45556</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19472295$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hu, Yiyi</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Duan, Huiqin</creatorcontrib><creatorcontrib>Hu, Yuanliang</creatorcontrib><creatorcontrib>Mu, Xiang</creatorcontrib><title>Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐α (TNF‐α), and interleukin‐1α (IL‐1α) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml−1 for 3 h and then treated with PD at 1, 5, and 10 mg ml−1 and its seven ingredients at 1, 5, and 10 µg ml−1 for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B4, berberine, jatrorrhizine, and aesculin downregulated TNF‐α expression; PD, anemoside B4, berberine, and palmatine decreased the content of IL‐1α. It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐α, and IL‐1α in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd.</description><subject>Animals</subject><subject>Cells</subject><subject>Cytokines - metabolism</subject><subject>Drugs, Chinese Herbal - chemistry</subject><subject>Drugs, Chinese Herbal - pharmacology</subject><subject>Endothelial Cells - drug effects</subject><subject>Endothelial Cells - metabolism</subject><subject>Endothelin-1 - metabolism</subject><subject>Endothelium, Vascular - cytology</subject><subject>Endothelium, Vascular - metabolism</subject><subject>ET-1</subject><subject>IL-1α</subject><subject>Inflammation Mediators - metabolism</subject><subject>Interleukin-1alpha - metabolism</subject><subject>Intestines - blood supply</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>LPS</subject><subject>Nitric Oxide - biosynthesis</subject><subject>Nitric Oxide - metabolism</subject><subject>Plant Preparations - chemistry</subject><subject>Plant Preparations - pharmacology</subject><subject>Pulsatilla</subject><subject>Pulsatilla - chemistry</subject><subject>Rats</subject><subject>RIMECs</subject><subject>TNF-α</subject><subject>Tumor Necrosis Factor-alpha - metabolism</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAQhyMEoktB4gmQT4jDuvhvEh_pqrsURduKLuJoOc6EGrxJsZPSPk2foS_SZ8LbjeCEkA-WZz5_mtEvy15TckQJYe9t3R5RWZAn2YwSpTAphXiazQjLOc5FKQ6yFzF-J4SonJPn2QFVomBMyVl2dz76aAbnvUEN2N4Oru-Q6RrkhohMel4Dct23AI2DLpVcd-lqN6AINsAj3LdofTZHJxtM52izXuKH-_mj4bTC9OE-_UDV-QV2XTNaaFAwQyoNEAfXGY-2zob-2kQ7ehMQdE0_XIJ3qWPB-_gye9YaH-HVdB9mX5Ynm8VHXJ2tThcfKmx5WRIsqaStJIqxWsqcQy0Z45yKxog8L0huOFdQEFVKJRQIUZeqLojlhawL1oDih9nbvfcq9D_HNJzeuribwHTQj1HnhWCcJun_QEaKdPId-G4Ppv1iDNDqq-C2JtxqSvQuNZ1S07vUEvpmco71Fpq_4BRTAvAe-OU83P5TpBfHy0k48S4OcPOHN-FHWiQtrb-uV1ocf_6kLlZcV_w3Tn6vgw</recordid><startdate>200907</startdate><enddate>200907</enddate><creator>Hu, Yiyi</creator><creator>Chen, Xi</creator><creator>Duan, Huiqin</creator><creator>Hu, Yuanliang</creator><creator>Mu, Xiang</creator><general>John Wiley & Sons, Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>200907</creationdate><title>Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells</title><author>Hu, Yiyi ; Chen, Xi ; Duan, Huiqin ; Hu, Yuanliang ; Mu, Xiang</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3880-5151f50922b5563eb5223314da466706a339e70985949e44b89b70c375b72de93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Animals</topic><topic>Cells</topic><topic>Cytokines - metabolism</topic><topic>Drugs, Chinese Herbal - chemistry</topic><topic>Drugs, Chinese Herbal - pharmacology</topic><topic>Endothelial Cells - drug effects</topic><topic>Endothelial Cells - metabolism</topic><topic>Endothelin-1 - metabolism</topic><topic>Endothelium, Vascular - cytology</topic><topic>Endothelium, Vascular - metabolism</topic><topic>ET-1</topic><topic>IL-1α</topic><topic>Inflammation Mediators - metabolism</topic><topic>Interleukin-1alpha - metabolism</topic><topic>Intestines - blood supply</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>LPS</topic><topic>Nitric Oxide - biosynthesis</topic><topic>Nitric Oxide - metabolism</topic><topic>Plant Preparations - chemistry</topic><topic>Plant Preparations - pharmacology</topic><topic>Pulsatilla</topic><topic>Pulsatilla - chemistry</topic><topic>Rats</topic><topic>RIMECs</topic><topic>TNF-α</topic><topic>Tumor Necrosis Factor-alpha - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hu, Yiyi</creatorcontrib><creatorcontrib>Chen, Xi</creatorcontrib><creatorcontrib>Duan, Huiqin</creatorcontrib><creatorcontrib>Hu, Yuanliang</creatorcontrib><creatorcontrib>Mu, Xiang</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hu, Yiyi</au><au>Chen, Xi</au><au>Duan, Huiqin</au><au>Hu, Yuanliang</au><au>Mu, Xiang</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>2009-07</date><risdate>2009</risdate><volume>27</volume><issue>5</issue><spage>284</spage><epage>288</epage><pages>284-288</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><abstract>To investigate the pharmacological mechanism of the traditional Chinese medicine, Pulsatilla decoction (PD), the levels of nitric oxide (NO), endothelin‐1 (ET‐1), tumor necrosis factor‐α (TNF‐α), and interleukin‐1α (IL‐1α) secreted by cultured rat intestinal microvascular endothelial cells (RIMECs) were determined after treatment with PD and its seven active ingredients, namely anemoside B4, anemonin, berberine, jatrorrhizine, palmatine, aesculin, and esculetin. RIMECs were challenged with lipopolysaccharide (LPS) at 1 µg ml−1 for 3 h and then treated with PD at 1, 5, and 10 mg ml−1 and its seven ingredients at 1, 5, and 10 µg ml−1 for 21 h, respectively. The results revealed that PD, anemonin, berberine, and esculetin inhibited the production of NO; PD, anemonin, and esculetin inhibited the secretion of ET‐1; PD, anemoside B4, berberine, jatrorrhizine, and aesculin downregulated TNF‐α expression; PD, anemoside B4, berberine, and palmatine decreased the content of IL‐1α. It showed that PD and its active ingredients could significantly inhibit the secretion of NO, ET‐1, TNF‐α, and IL‐1α in LPS‐induced RIMECs and suggested they would reduce inflammatory response via these cytokines. Copyright © 2009 John Wiley & Sons, Ltd.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>19472295</pmid><doi>10.1002/cbf.1570</doi><tpages>5</tpages></addata></record> |
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| ispartof | Cell biochemistry and function, 2009-07, Vol.27 (5), p.284-288 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Animals Cells Cytokines - metabolism Drugs, Chinese Herbal - chemistry Drugs, Chinese Herbal - pharmacology Endothelial Cells - drug effects Endothelial Cells - metabolism Endothelin-1 - metabolism Endothelium, Vascular - cytology Endothelium, Vascular - metabolism ET-1 IL-1α Inflammation Mediators - metabolism Interleukin-1alpha - metabolism Intestines - blood supply Lipopolysaccharides - pharmacology LPS Nitric Oxide - biosynthesis Nitric Oxide - metabolism Plant Preparations - chemistry Plant Preparations - pharmacology Pulsatilla Pulsatilla - chemistry Rats RIMECs TNF-α Tumor Necrosis Factor-alpha - metabolism |
| title | Pulsatilla decoction and its active ingredients inhibit secretion of NO, ET-1, TNF-α, and IL-1α in LPS-induced rat intestinal microvascular endothelial cells |
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