CpG-DNA protects against a lethal orthopoxvirus infection in a murine model
CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection w...
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| Veröffentlicht in: | Antiviral research 2005-02, Vol.65 (2), p.87-95 |
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| creator | Rees, D.G. Cerys Gates, Amanda J. Green, Michael Eastaugh, Lin Lukaszewski, Roman A. Griffin, Kate F. Krieg, Arthur M. Titball, Richard W. |
| description | CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection with an orthopoxvirus (vaccinia virus). Dosing with either CpG-A or B by the intraperitonal or intranasal route protected mice against a subsequent intranasal challenge with vaccinia virus. To our knowledge, this is the first time CpG-mediated protection has been demonstrated at the lung surface. The level of protection was greater when CpG-DNA was administered intranasally demonstrating a clear relationship between the route of CpG dosing and infection route. Treatment with CpG-B reduced viral titer in the lung by 10,000-fold at day 3 post-infection. The CC chemokines RANTES and MIP-1β were elevated in the broncho-alveolar lavage from animals treated intranasally with CpG-B compared to untreated and intraperitoneally dosed controls, and it is possible that these chemokines play a role in the clearance of intranasally delivered vaccinia virus. |
| doi_str_mv | 10.1016/j.antiviral.2004.10.004 |
| format | Article |
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Cerys ; Gates, Amanda J. ; Green, Michael ; Eastaugh, Lin ; Lukaszewski, Roman A. ; Griffin, Kate F. ; Krieg, Arthur M. ; Titball, Richard W.</creator><creatorcontrib>Rees, D.G. Cerys ; Gates, Amanda J. ; Green, Michael ; Eastaugh, Lin ; Lukaszewski, Roman A. ; Griffin, Kate F. ; Krieg, Arthur M. ; Titball, Richard W.</creatorcontrib><description>CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection with an orthopoxvirus (vaccinia virus). Dosing with either CpG-A or B by the intraperitonal or intranasal route protected mice against a subsequent intranasal challenge with vaccinia virus. To our knowledge, this is the first time CpG-mediated protection has been demonstrated at the lung surface. The level of protection was greater when CpG-DNA was administered intranasally demonstrating a clear relationship between the route of CpG dosing and infection route. Treatment with CpG-B reduced viral titer in the lung by 10,000-fold at day 3 post-infection. The CC chemokines RANTES and MIP-1β were elevated in the broncho-alveolar lavage from animals treated intranasally with CpG-B compared to untreated and intraperitoneally dosed controls, and it is possible that these chemokines play a role in the clearance of intranasally delivered vaccinia virus.</description><identifier>ISSN: 0166-3542</identifier><identifier>EISSN: 1872-9096</identifier><identifier>DOI: 10.1016/j.antiviral.2004.10.004</identifier><identifier>PMID: 15708635</identifier><identifier>CODEN: ARSRDR</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Antibiotics. Antiinfectious agents. 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Cerys</creatorcontrib><creatorcontrib>Gates, Amanda J.</creatorcontrib><creatorcontrib>Green, Michael</creatorcontrib><creatorcontrib>Eastaugh, Lin</creatorcontrib><creatorcontrib>Lukaszewski, Roman A.</creatorcontrib><creatorcontrib>Griffin, Kate F.</creatorcontrib><creatorcontrib>Krieg, Arthur M.</creatorcontrib><creatorcontrib>Titball, Richard W.</creatorcontrib><title>CpG-DNA protects against a lethal orthopoxvirus infection in a murine model</title><title>Antiviral research</title><addtitle>Antiviral Res</addtitle><description>CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection with an orthopoxvirus (vaccinia virus). Dosing with either CpG-A or B by the intraperitonal or intranasal route protected mice against a subsequent intranasal challenge with vaccinia virus. To our knowledge, this is the first time CpG-mediated protection has been demonstrated at the lung surface. The level of protection was greater when CpG-DNA was administered intranasally demonstrating a clear relationship between the route of CpG dosing and infection route. Treatment with CpG-B reduced viral titer in the lung by 10,000-fold at day 3 post-infection. The CC chemokines RANTES and MIP-1β were elevated in the broncho-alveolar lavage from animals treated intranasally with CpG-B compared to untreated and intraperitoneally dosed controls, and it is possible that these chemokines play a role in the clearance of intranasally delivered vaccinia virus.</description><subject>Animals</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Antiviral agents</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chemokines - biosynthesis</subject><subject>CpG-DNA</subject><subject>Cytokines - biosynthesis</subject><subject>Disease Models, Animal</subject><subject>Female</subject><subject>Innate immunity</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Oligodeoxyribonucleotides - genetics</subject><subject>Oligodeoxyribonucleotides - pharmacology</subject><subject>Orthopoxvirus</subject><subject>Pharmacology. Drug treatments</subject><subject>Poxviridae Infections - immunology</subject><subject>Poxviridae Infections - prevention & control</subject><subject>Poxviridae Infections - virology</subject><subject>Vaccinia virus</subject><subject>Vaccinia virus - immunology</subject><subject>Vaccinia virus - pathogenicity</subject><subject>Vaccinia virus - physiology</subject><subject>Virus Replication - drug effects</subject><issn>0166-3542</issn><issn>1872-9096</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkMtOwzAQRS0EgvL4BcgGdim2azvJsipPgWADa2tiT8BVEhc7QfD3uGoFS1Zjec7MXB1CzhidMsrU5XIK_eA-XYB2yikV6Xeayg6ZsLLgeUUrtUsmiVT5TAp-QA5jXFJKVVGV--SAyYKWaiYn5GGxus2vnubZKvgBzRAzeAPXxyGDrMXhHdrMh-Hdr_xXujbGzPVNwpzv0ysx3Rhcj1nnLbbHZK-BNuLJth6R15vrl8Vd_vh8e7-YP-ZGVGrIG-BlI9BSg6WksqgZN0ZVUnHKhQGAuq6tArDS1ExKbGbCMpBWYckKQavZEbnY7E2ZP0aMg-5cNNi20KMfo1aF4LyU_F8wrSsFVSKBxQY0wccYsNGr4DoI35pRvRaul_pXuF4LXzdSSZOn2xNj3aH9m9saTsD5FoBooG0C9MbFP05JXkm-zjrfcJjMfToMOhqHvUHrQhKurXf_hvkB3gqjWQ</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Rees, D.G. 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Antiparasitic agents</topic><topic>Antiviral agents</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chemokines - biosynthesis</topic><topic>CpG-DNA</topic><topic>Cytokines - biosynthesis</topic><topic>Disease Models, Animal</topic><topic>Female</topic><topic>Innate immunity</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Oligodeoxyribonucleotides - genetics</topic><topic>Oligodeoxyribonucleotides - pharmacology</topic><topic>Orthopoxvirus</topic><topic>Pharmacology. 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Cerys</au><au>Gates, Amanda J.</au><au>Green, Michael</au><au>Eastaugh, Lin</au><au>Lukaszewski, Roman A.</au><au>Griffin, Kate F.</au><au>Krieg, Arthur M.</au><au>Titball, Richard W.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>CpG-DNA protects against a lethal orthopoxvirus infection in a murine model</atitle><jtitle>Antiviral research</jtitle><addtitle>Antiviral Res</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>65</volume><issue>2</issue><spage>87</spage><epage>95</epage><pages>87-95</pages><issn>0166-3542</issn><eissn>1872-9096</eissn><coden>ARSRDR</coden><abstract>CpG-DNA has been described as a potent activator of the innate immune system, with potential to protect against infection caused by a range of pathogens in a non-specific manner. Here two classes of CpG-DNA (CpG-A and CpG-B) have been investigated for their abilities to protect mice from infection with an orthopoxvirus (vaccinia virus). Dosing with either CpG-A or B by the intraperitonal or intranasal route protected mice against a subsequent intranasal challenge with vaccinia virus. To our knowledge, this is the first time CpG-mediated protection has been demonstrated at the lung surface. The level of protection was greater when CpG-DNA was administered intranasally demonstrating a clear relationship between the route of CpG dosing and infection route. Treatment with CpG-B reduced viral titer in the lung by 10,000-fold at day 3 post-infection. The CC chemokines RANTES and MIP-1β were elevated in the broncho-alveolar lavage from animals treated intranasally with CpG-B compared to untreated and intraperitoneally dosed controls, and it is possible that these chemokines play a role in the clearance of intranasally delivered vaccinia virus.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15708635</pmid><doi>10.1016/j.antiviral.2004.10.004</doi><tpages>9</tpages></addata></record> |
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| ispartof | Antiviral research, 2005-02, Vol.65 (2), p.87-95 |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Antibiotics. Antiinfectious agents. Antiparasitic agents Antiviral agents Base Sequence Biological and medical sciences Chemokines - biosynthesis CpG-DNA Cytokines - biosynthesis Disease Models, Animal Female Innate immunity Medical sciences Mice Mice, Inbred BALB C Oligodeoxyribonucleotides - genetics Oligodeoxyribonucleotides - pharmacology Orthopoxvirus Pharmacology. Drug treatments Poxviridae Infections - immunology Poxviridae Infections - prevention & control Poxviridae Infections - virology Vaccinia virus Vaccinia virus - immunology Vaccinia virus - pathogenicity Vaccinia virus - physiology Virus Replication - drug effects |
| title | CpG-DNA protects against a lethal orthopoxvirus infection in a murine model |
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