alternative tandem affinity purification strategy applied to Arabidopsis protein complex isolation

Tandem affinity purification (TAP) strategies constitute an efficient approach for protein complex purification from many different organisms. However, the application of such strategies for purifying endogenous Arabidopsis multi-protein complexes has not yet been reported. Here, we describe an alte...

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Veröffentlicht in:	The Plant journal : for cell and molecular biology 2005-03, Vol.41 (5), p.767-778
Hauptverfasser:	Rubio, V, Shen, Y, Saijo, Y, Liu, Y, Gusmaroli, G, Dinesh-Kumar, S.P, Deng, X.D
Format:	Artikel
Sprache:	eng
Schlagworte:	Agronomy. Soil science and plant productions Amino Acid Sequence amino acid sequences Arabidopsis Arabidopsis - metabolism Arabidopsis Proteins - chemistry Arabidopsis Proteins - isolation & purification Arabidopsis Proteins - metabolism Arabidopsis thaliana Biological and medical sciences Chromatography, Affinity Classical and quantitative genetics. Population genetics. Molecular genetics CSN CSN3 Fundamental and applied biological sciences. Psychology Gene expression Generalities. Genetics. Plant material Genetics and breeding of economic plants Macromolecular Substances - isolation & purification Models, Biological Molecular genetics Molecular Sequence Data Pathogens Plant pathology Plant populations plant proteins protein complex purification protein tagging Proteins Rhinovirus tandem affinity purification TAP Tobacco etch virus transgenic plants
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