rapid and efficient method for isolation of total RNA from Euglena gracilis (Euglenoidea)

RNA isolation is essential to the study of gene expression at the molecular level. However, it is difficult to isolate RNA from organisms that contain large amounts of polysaccharides or other compounds that bind or coprecipitate with RNA, such as the unicellular protist Euglena gracilis. Currently,...

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Veröffentlicht in:	Genetics and molecular research 2009-01, Vol.8 (2), p.482-486
Hauptverfasser:	González-Mendoza, D, Morales-Trejo, A, Brito-Vera, H
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Euglena gracilis Euglena gracilis - genetics Phenol - chemistry polysaccharides Reverse Transcriptase Polymerase Chain Reaction RNA RNA, Protozoan - chemistry RNA, Protozoan - isolation & purification sodium dodecyl sulfate Sodium Dodecyl Sulfate - chemistry
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