Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi
To determine whether Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model. A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal...
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| Veröffentlicht in: | American journal of obstetrics and gynecology 2005-02, Vol.192 (2), p.586-591 |
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| container_title | American journal of obstetrics and gynecology |
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| creator | Shippey, Stuart H. Zahn, Christopher M. Cisar, Margaret M. Wu, T. John Satin, Andrew J. |
| description | To determine whether
Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model.
A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically.
Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24–48 hours. Following placental perfusion,
T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative.
This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta. |
| doi_str_mv | 10.1016/j.ajog.2004.07.079 |
| format | Article |
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Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model.
A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically.
Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24–48 hours. Following placental perfusion,
T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative.
This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta.</description><identifier>ISSN: 0002-9378</identifier><identifier>EISSN: 1097-6868</identifier><identifier>DOI: 10.1016/j.ajog.2004.07.079</identifier><identifier>PMID: 15696007</identifier><identifier>CODEN: AJOGAH</identifier><language>eng</language><publisher>Philadelphia, PA: Mosby, Inc</publisher><subject>Animals ; Biological and medical sciences ; Cells, Cultured ; Chagas Disease - congenital ; Chagas Disease - transmission ; Congenital Chagas' disease ; Diseases of mother, fetus and pregnancy ; DNA, Protozoan - analysis ; Female ; Gynecology. Andrology. Obstetrics ; Human protozoal diseases ; Humans ; Infectious Disease Transmission, Vertical ; Infectious diseases ; Medical sciences ; Parasitic diseases ; Perfusion ; Placenta - parasitology ; Placental perfusion model ; Pregnancy ; Pregnancy. Fetus. Placenta ; Protozoal diseases ; Transplacental infection ; Trophoblasts - parasitology ; Trypanosoma cruzi ; Trypanosoma cruzi - isolation & purification ; Trypanosomiasis</subject><ispartof>American journal of obstetrics and gynecology, 2005-02, Vol.192 (2), p.586-591</ispartof><rights>2005 Elsevier Inc.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c384t-d749fd1565c66649c6ebb8a4d9052b4f22b3be1d7febb5449460017dea143a363</citedby><cites>FETCH-LOGICAL-c384t-d749fd1565c66649c6ebb8a4d9052b4f22b3be1d7febb5449460017dea143a363</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/S0002937804008634$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3537,27901,27902,65306</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16524588$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15696007$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shippey, Stuart H.</creatorcontrib><creatorcontrib>Zahn, Christopher M.</creatorcontrib><creatorcontrib>Cisar, Margaret M.</creatorcontrib><creatorcontrib>Wu, T. John</creatorcontrib><creatorcontrib>Satin, Andrew J.</creatorcontrib><title>Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi</title><title>American journal of obstetrics and gynecology</title><addtitle>Am J Obstet Gynecol</addtitle><description>To determine whether
Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model.
A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically.
Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24–48 hours. Following placental perfusion,
T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative.
This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chagas Disease - congenital</subject><subject>Chagas Disease - transmission</subject><subject>Congenital Chagas' disease</subject><subject>Diseases of mother, fetus and pregnancy</subject><subject>DNA, Protozoan - analysis</subject><subject>Female</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Human protozoal diseases</subject><subject>Humans</subject><subject>Infectious Disease Transmission, Vertical</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Parasitic diseases</subject><subject>Perfusion</subject><subject>Placenta - parasitology</subject><subject>Placental perfusion model</subject><subject>Pregnancy</subject><subject>Pregnancy. Fetus. Placenta</subject><subject>Protozoal diseases</subject><subject>Transplacental infection</subject><subject>Trophoblasts - parasitology</subject><subject>Trypanosoma cruzi</subject><subject>Trypanosoma cruzi - isolation & purification</subject><subject>Trypanosomiasis</subject><issn>0002-9378</issn><issn>1097-6868</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFq3DAQhkVpSbZJXqCHokt781aSZcmCXkpo2kCgl-QWEGNpnGqxLVeyA8nTV5tdSE-FAaHhm5-fj5APnG054-rLbgu7-LAVjMkt02XMG7LhzOhKtap9SzaMMVGZWren5H3Ou_1XGHFCTnmjjGJMb8j9XUYae7r8RjoP4HBaYKAzpn7NIU50jB4HukSKjzCssCBdEkz5HxRyhoeXjNv0NMMUcxyBurQ-h3Pyroch48XxPSN3V99vL39WN79-XF9-u6lc3cql8lqa3pdOjVNKSeMUdl0L0hvWiE72QnR1h9zrvuwbKY0s3bn2CFzWUKv6jHw-5M4p_lkxL3YM2eEwwIRxzVZpyUWtmwKKA-hSzDlhb-cURkhPljO7d2p3du_U7p1apsuYcvTxmL52I_rXk6PEAnw6ApAdDH0R5EJ-5VQjZNO2hft64LC4eAyYbHYBJ4c-JHSL9TH8r8dfJu-WGQ</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Shippey, Stuart H.</creator><creator>Zahn, Christopher M.</creator><creator>Cisar, Margaret M.</creator><creator>Wu, T. John</creator><creator>Satin, Andrew J.</creator><general>Mosby, Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi</title><author>Shippey, Stuart H. ; Zahn, Christopher M. ; Cisar, Margaret M. ; Wu, T. John ; Satin, Andrew J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c384t-d749fd1565c66649c6ebb8a4d9052b4f22b3be1d7febb5449460017dea143a363</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chagas Disease - congenital</topic><topic>Chagas Disease - transmission</topic><topic>Congenital Chagas' disease</topic><topic>Diseases of mother, fetus and pregnancy</topic><topic>DNA, Protozoan - analysis</topic><topic>Female</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Human protozoal diseases</topic><topic>Humans</topic><topic>Infectious Disease Transmission, Vertical</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Parasitic diseases</topic><topic>Perfusion</topic><topic>Placenta - parasitology</topic><topic>Placental perfusion model</topic><topic>Pregnancy</topic><topic>Pregnancy. Fetus. Placenta</topic><topic>Protozoal diseases</topic><topic>Transplacental infection</topic><topic>Trophoblasts - parasitology</topic><topic>Trypanosoma cruzi</topic><topic>Trypanosoma cruzi - isolation & purification</topic><topic>Trypanosomiasis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shippey, Stuart H.</creatorcontrib><creatorcontrib>Zahn, Christopher M.</creatorcontrib><creatorcontrib>Cisar, Margaret M.</creatorcontrib><creatorcontrib>Wu, T. John</creatorcontrib><creatorcontrib>Satin, Andrew J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>American journal of obstetrics and gynecology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shippey, Stuart H.</au><au>Zahn, Christopher M.</au><au>Cisar, Margaret M.</au><au>Wu, T. John</au><au>Satin, Andrew J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi</atitle><jtitle>American journal of obstetrics and gynecology</jtitle><addtitle>Am J Obstet Gynecol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>192</volume><issue>2</issue><spage>586</spage><epage>591</epage><pages>586-591</pages><issn>0002-9378</issn><eissn>1097-6868</eissn><coden>AJOGAH</coden><abstract>To determine whether
Trypanosoma cruzi could be identified in placental cells in vitro and in placental tissue using a human ex vivo perfusion model.
A placental cell line was incubated with trypomastigotes for progressive time periods. Trypomastigotes were also infused as a bolus into the maternal circulation of a placental perfusion model. Maternal and fetal perfusates, and placental tissue, were analyzed for parasitic DNA using polymerase chain reaction; perfused specimens were also examined histologically.
Intracytoplasmic amastigotes were identified in trophoblast of the incubated cell line by 24–48 hours. Following placental perfusion,
T cruzi DNA was identified in all postinoculation maternal perfusate samples and postinoculation placental tissue specimens; preinoculation controls were negative.
This is the first description of the use of the human placental perfusion model to study congenital Chagas' disease, including the presence and time course of early parasitic invasion of the placenta.</abstract><cop>Philadelphia, PA</cop><pub>Mosby, Inc</pub><pmid>15696007</pmid><doi>10.1016/j.ajog.2004.07.079</doi><tpages>6</tpages></addata></record> |
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| ispartof | American journal of obstetrics and gynecology, 2005-02, Vol.192 (2), p.586-591 |
| issn | 0002-9378 1097-6868 |
| language | eng |
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| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Animals Biological and medical sciences Cells, Cultured Chagas Disease - congenital Chagas Disease - transmission Congenital Chagas' disease Diseases of mother, fetus and pregnancy DNA, Protozoan - analysis Female Gynecology. Andrology. Obstetrics Human protozoal diseases Humans Infectious Disease Transmission, Vertical Infectious diseases Medical sciences Parasitic diseases Perfusion Placenta - parasitology Placental perfusion model Pregnancy Pregnancy. Fetus. Placenta Protozoal diseases Transplacental infection Trophoblasts - parasitology Trypanosoma cruzi Trypanosoma cruzi - isolation & purification Trypanosomiasis |
| title | Use of the placental perfusion model to evaluate transplacental passage of Trypanosoma cruzi |
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