Detection of Subepithelial Fibrosis Associated with Corneal Stromal Edema by Second Harmonic Generation Imaging Microscopy

Human corneas with or without stromal edema were examined by second harmonic generation (SHG) imaging microscopy to characterize stromal collagen organization. Tissue buttons from 31 corneas with stromal edema and 8 normal corneas were fixed, and 3-mm(2) blocks were cut and stained with phalloidin,...

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Veröffentlicht in:	Investigative ophthalmology & visual science 2009-07, Vol.50 (7), p.3145-3150
Hauptverfasser:	Morishige, Naoyuki, Yamada, Naoyuki, Teranishi, Shinichiro, Chikama, Tai-ichiro, Nishida, Teruo, Takahara, Atsushi
Format:	Artikel
Sprache:	eng
Schlagworte:	Aged Aged, 80 and over Biological and medical sciences Collagen - metabolism Corneal Edema - diagnosis Corneal Edema - surgery Corneal Stroma - metabolism Corneal Stroma - pathology Cytoskeleton - metabolism Cytoskeleton - pathology Diseases of cornea, anterior segment and sclera Epithelium, Corneal - pathology Eye and associated structures. Visual pathways and centers. Vision Fibroblasts - pathology Fibrosis - diagnosis Fuchs' Endothelial Dystrophy - pathology Fuchs' Endothelial Dystrophy - surgery Fundamental and applied biological sciences. Psychology Humans Image Processing, Computer-Assisted Keratoplasty, Penetrating Medical sciences Microscopy, Confocal Middle Aged Ophthalmology Time Factors Vertebrates: nervous system and sense organs
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