Pseudomonas Invasion of Type I Pneumocytes Is Dependent on the Expression and Phosphorylation of Caveolin-2
Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a m...
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| Veröffentlicht in: | The Journal of biological chemistry 2005-02, Vol.280 (6), p.4864-4872 |
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| container_title | The Journal of biological chemistry |
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| creator | Zaas, David W. Duncan, Mathew J. Li, Guojie Wright, Jo Rae Abraham, Soman N. |
| description | Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion. |
| doi_str_mv | 10.1074/jbc.M411702200 |
| format | Article |
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Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion.</description><identifier>ISSN: 0021-9258</identifier><identifier>EISSN: 1083-351X</identifier><identifier>DOI: 10.1074/jbc.M411702200</identifier><identifier>PMID: 15545264</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Animals ; Caveolin 1 ; Caveolin 2 ; Caveolins - metabolism ; Cell Adhesion ; Cell Line ; Cells, Cultured ; Coloring Agents - pharmacology ; Cystic Fibrosis - metabolism ; Epithelial Cells - microbiology ; Immunoprecipitation ; Lipid Metabolism ; Lipids - chemistry ; Lung - cytology ; Lung - microbiology ; Lung - pathology ; Membrane Microdomains - chemistry ; Membrane Microdomains - metabolism ; Mice ; Microscopy, Confocal ; Microscopy, Electron ; Phosphorylation ; Pseudomonas - metabolism ; Pulmonary Alveoli - metabolism ; Rats ; Rats, Sprague-Dawley ; RNA Interference ; Tetrazolium Salts - pharmacology ; Thiazoles - pharmacology ; Tyrosine - chemistry</subject><ispartof>The Journal of biological chemistry, 2005-02, Vol.280 (6), p.4864-4872</ispartof><rights>2005 © 2005 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology.</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c440t-430c0bdf7a082d07f69b6520874ccdf2bf4bbd2a5e557bc5d7881c0f478ecc453</citedby><cites>FETCH-LOGICAL-c440t-430c0bdf7a082d07f69b6520874ccdf2bf4bbd2a5e557bc5d7881c0f478ecc453</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15545264$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Zaas, David W.</creatorcontrib><creatorcontrib>Duncan, Mathew J.</creatorcontrib><creatorcontrib>Li, Guojie</creatorcontrib><creatorcontrib>Wright, Jo Rae</creatorcontrib><creatorcontrib>Abraham, Soman N.</creatorcontrib><title>Pseudomonas Invasion of Type I Pneumocytes Is Dependent on the Expression and Phosphorylation of Caveolin-2</title><title>The Journal of biological chemistry</title><addtitle>J Biol Chem</addtitle><description>Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion.</description><subject>Animals</subject><subject>Caveolin 1</subject><subject>Caveolin 2</subject><subject>Caveolins - metabolism</subject><subject>Cell Adhesion</subject><subject>Cell Line</subject><subject>Cells, Cultured</subject><subject>Coloring Agents - pharmacology</subject><subject>Cystic Fibrosis - metabolism</subject><subject>Epithelial Cells - microbiology</subject><subject>Immunoprecipitation</subject><subject>Lipid Metabolism</subject><subject>Lipids - chemistry</subject><subject>Lung - cytology</subject><subject>Lung - microbiology</subject><subject>Lung - pathology</subject><subject>Membrane Microdomains - chemistry</subject><subject>Membrane Microdomains - metabolism</subject><subject>Mice</subject><subject>Microscopy, Confocal</subject><subject>Microscopy, Electron</subject><subject>Phosphorylation</subject><subject>Pseudomonas - metabolism</subject><subject>Pulmonary Alveoli - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA Interference</subject><subject>Tetrazolium Salts - pharmacology</subject><subject>Thiazoles - pharmacology</subject><subject>Tyrosine - chemistry</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1v1DAQxS0EokvhyhFFHLhlGTt27D2ibYGVithDkbhZ_pgQl8QOcbKw_z1pd6WeEHOZw_ze0-g9Ql5TWFOQ_P2ddesvnFIJjAE8ISsKqiorQb8_JSsARssNE-qCvMj5DpbhG_qcXFAhuGA1X5Gf-4yzT32KJhe7eDA5pFikprg9Dljsin3EuU_uOOFyzsUVDhg9xqlYqKnF4vrPMGJ-EJnoi32b8tCm8diZ6Wy0NQdMXYgle0meNabL-Oq8L8m3j9e328_lzddPu-2Hm9JxDlPJK3BgfSMNKOZBNvXG1oKBktw53zDbcGs9MwKFkNYJL5WiDhouFTrHRXVJ3p18hzH9mjFPug_ZYdeZiGnOupYcJHD5X5DKmkMF947rE-jGlPOIjR7G0JvxqCno-x700oN-7GERvDk7z7ZH_4ifg1-AtyegDT_a32FEbUNyLfaaKdC15uoBUicIl7QOAUedXcDo0C8CN2mfwr8e-Avl-6J7</recordid><startdate>20050211</startdate><enddate>20050211</enddate><creator>Zaas, David W.</creator><creator>Duncan, Mathew J.</creator><creator>Li, Guojie</creator><creator>Wright, Jo Rae</creator><creator>Abraham, Soman N.</creator><general>Elsevier Inc</general><general>American Society for Biochemistry and Molecular Biology</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050211</creationdate><title>Pseudomonas Invasion of Type I Pneumocytes Is Dependent on the Expression and Phosphorylation of Caveolin-2</title><author>Zaas, David W. ; Duncan, Mathew J. ; Li, Guojie ; Wright, Jo Rae ; Abraham, Soman N.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c440t-430c0bdf7a082d07f69b6520874ccdf2bf4bbd2a5e557bc5d7881c0f478ecc453</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Caveolin 1</topic><topic>Caveolin 2</topic><topic>Caveolins - metabolism</topic><topic>Cell Adhesion</topic><topic>Cell Line</topic><topic>Cells, Cultured</topic><topic>Coloring Agents - pharmacology</topic><topic>Cystic Fibrosis - metabolism</topic><topic>Epithelial Cells - microbiology</topic><topic>Immunoprecipitation</topic><topic>Lipid Metabolism</topic><topic>Lipids - chemistry</topic><topic>Lung - cytology</topic><topic>Lung - microbiology</topic><topic>Lung - pathology</topic><topic>Membrane Microdomains - chemistry</topic><topic>Membrane Microdomains - metabolism</topic><topic>Mice</topic><topic>Microscopy, Confocal</topic><topic>Microscopy, Electron</topic><topic>Phosphorylation</topic><topic>Pseudomonas - metabolism</topic><topic>Pulmonary Alveoli - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA Interference</topic><topic>Tetrazolium Salts - pharmacology</topic><topic>Thiazoles - pharmacology</topic><topic>Tyrosine - chemistry</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Zaas, David W.</creatorcontrib><creatorcontrib>Duncan, Mathew J.</creatorcontrib><creatorcontrib>Li, Guojie</creatorcontrib><creatorcontrib>Wright, Jo Rae</creatorcontrib><creatorcontrib>Abraham, Soman N.</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Zaas, David W.</au><au>Duncan, Mathew J.</au><au>Li, Guojie</au><au>Wright, Jo Rae</au><au>Abraham, Soman N.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Pseudomonas Invasion of Type I Pneumocytes Is Dependent on the Expression and Phosphorylation of Caveolin-2</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>2005-02-11</date><risdate>2005</risdate><volume>280</volume><issue>6</issue><spage>4864</spage><epage>4872</epage><pages>4864-4872</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><abstract>Pseudomonas aeruginosa is a major cause of pneumonia in patients with cystic fibrosis and other immuncompromising conditions. Here we showed that P. aeruginosa invades type I pneumocytes via a lipid raft-mediated mechanism. P. aeruginosa invasion of rat primary type I-like pneumocytes as well as a murine lung epithelial cell line 12 (MLE-12) is inhibited by drugs that remove membrane cholesterol and disrupt lipid rafts. Confocal microscopy demonstrated co-localization of intracellular P. aeruginosa with lipid raft components including caveolin-1 and -2. We generated caveolin-1 and -2 knockdowns in MLE-12 cells by using RNA interference techniques. Decreased expression of caveolin-2 significantly impaired the ability of P. aeruginosa to invade MLE-12 cells. In addition, the lipid raft-dependent tyrosine phosphorylation of caveolin-2 appeared to be a critical regulator of P. aeruginosa invasion.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15545264</pmid><doi>10.1074/jbc.M411702200</doi><tpages>9</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Animals Caveolin 1 Caveolin 2 Caveolins - metabolism Cell Adhesion Cell Line Cells, Cultured Coloring Agents - pharmacology Cystic Fibrosis - metabolism Epithelial Cells - microbiology Immunoprecipitation Lipid Metabolism Lipids - chemistry Lung - cytology Lung - microbiology Lung - pathology Membrane Microdomains - chemistry Membrane Microdomains - metabolism Mice Microscopy, Confocal Microscopy, Electron Phosphorylation Pseudomonas - metabolism Pulmonary Alveoli - metabolism Rats Rats, Sprague-Dawley RNA Interference Tetrazolium Salts - pharmacology Thiazoles - pharmacology Tyrosine - chemistry |
| title | Pseudomonas Invasion of Type I Pneumocytes Is Dependent on the Expression and Phosphorylation of Caveolin-2 |
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