Mitochondrial trans-2-Enoyl-CoA Reductase of Wax Ester Fermentation from Euglena gracilis Defines a New Family of Enzymes Involved in Lipid Synthesis

Under anaerobiosis, Euglena gracilis mitochondria perform a malonyl-CoA independent synthesis of fatty acids leading to accumulation of wax esters, which serve as the sink for electrons stemming from glycolytic ATP synthesis and pyruvate oxidation. An important enzyme of this unusual pathway is tran...

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Veröffentlicht in:	The Journal of biological chemistry 2005-02, Vol.280 (6), p.4329-4338
Hauptverfasser:	Hoffmeister, Meike, Piotrowski, Markus, Nowitzki, Ulrich, Martin, William
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphate - chemistry Amino Acid Sequence Animals Base Sequence Blotting, Northern Blotting, Western Catalysis Chromatography Cloning, Molecular Culture Media DNA, Complementary - metabolism Electrons Electrophoresis, Polyacrylamide Gel Escherichia coli - metabolism Euglena gracilis - metabolism Evolution, Molecular Fatty Acids - metabolism Kinetics Lipid Metabolism Mass Spectrometry Mitochondria - enzymology Molecular Sequence Data NAD - metabolism NADH, NADPH Oxidoreductases - metabolism NADH, NADPH Oxidoreductases - physiology NADP - metabolism Nucleic Acid Hybridization Open Reading Frames Operon Oxidoreductases Acting on CH-CH Group Donors Peptides - chemistry Phylogeny Protein Binding Protein Structure, Tertiary Silver Staining Trypsin - chemistry
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