Relaxin Stimulates Interleukin-6 and Interleukin-8 Secretion from the Extraplacental Chorionic Cytotrophoblast
Abstract In the absence of infection, decidual relaxin (RLN) expression is increased in patients with preterm premature rupture of the membranes (PPROM) resulting in preterm birth, but it is not known whether inflammation stimulates RLN expression or vice versa. This study examined the effect of lip...
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| Veröffentlicht in: | Placenta (Eastbourne) 2009-07, Vol.30 (7), p.599-606 |
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| creator | Bryant-Greenwood, G.D Yamamoto, S.Y Sadowsky, D.W Gravett, M.G Novy, M.J |
| description | Abstract In the absence of infection, decidual relaxin (RLN) expression is increased in patients with preterm premature rupture of the membranes (PPROM) resulting in preterm birth, but it is not known whether inflammation stimulates RLN expression or vice versa. This study examined the effect of lipopolysaccharide (LPS) on the expression of RLN mRNA and secreted protein and whether RLN treatment influences secretion of proinflammatory cytokines from the fetal membranes. Explants of human fetal membranes in vitro and rhesus monkey fetal membranes in vivo were treated with LPS, which increased expression of IL-6 but had no effect on RLN. RLN treatment stimulated IL-6 and IL-8 secretion from choriodecidual explants in a subset of patients, as well as from isolated chorionic cytotrophoblast cells but not decidual cells. In vivo results obtained in rhesus monkeys after intra-amniotic infusion of RLN demonstrated increased IL-6 and IL-8 concentrations in amniotic fluid. Our results indicate that increased decidual RLN expression is independent of LPS but may induce a local sterile inflammatory process which potentially contributes to extracellular matrix degradation and weakening of the fetal membranes. |
| doi_str_mv | 10.1016/j.placenta.2009.04.009 |
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This study examined the effect of lipopolysaccharide (LPS) on the expression of RLN mRNA and secreted protein and whether RLN treatment influences secretion of proinflammatory cytokines from the fetal membranes. Explants of human fetal membranes in vitro and rhesus monkey fetal membranes in vivo were treated with LPS, which increased expression of IL-6 but had no effect on RLN. RLN treatment stimulated IL-6 and IL-8 secretion from choriodecidual explants in a subset of patients, as well as from isolated chorionic cytotrophoblast cells but not decidual cells. In vivo results obtained in rhesus monkeys after intra-amniotic infusion of RLN demonstrated increased IL-6 and IL-8 concentrations in amniotic fluid. Our results indicate that increased decidual RLN expression is independent of LPS but may induce a local sterile inflammatory process which potentially contributes to extracellular matrix degradation and weakening of the fetal membranes.</description><identifier>ISSN: 0143-4004</identifier><identifier>EISSN: 1532-3102</identifier><identifier>DOI: 10.1016/j.placenta.2009.04.009</identifier><identifier>PMID: 19467703</identifier><identifier>CODEN: PLACDF</identifier><language>eng</language><publisher>Kidlington: Elsevier Ltd</publisher><subject>Amniotic Fluid - metabolism ; Animals ; Biological and medical sciences ; Cells, Cultured ; Chorion - cytology ; Chorion - drug effects ; Chorion - secretion ; Decidua - cytology ; Decidua - drug effects ; Decidua - secretion ; Embryology: invertebrates and vertebrates. Teratology ; Extracellular Matrix - metabolism ; Extraembryonic Membranes - cytology ; Extraembryonic Membranes - drug effects ; Extraembryonic Membranes - secretion ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression - drug effects ; Gene Expression - physiology ; Humans ; Interleukin-6 - genetics ; Interleukin-6 - secretion ; Interleukin-8 - genetics ; Interleukin-8 - secretion ; Internal Medicine ; Lipopolysaccharides - pharmacology ; Macaca mulatta ; Obstetrics and Gynecology ; Pregnancy ; Preterm premature rupture of membranes ; Proinflammatory cytokines ; Recombinant Proteins - metabolism ; Recombinant Proteins - pharmacology ; Relaxin ; Relaxin - genetics ; Relaxin - metabolism ; Relaxin - pharmacology ; Trophoblasts - drug effects ; Trophoblasts - secretion</subject><ispartof>Placenta (Eastbourne), 2009-07, Vol.30 (7), p.599-606</ispartof><rights>Elsevier Ltd</rights><rights>2009 Elsevier Ltd</rights><rights>2009 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-43b7df0bed94bce63e197dd4d5d11a02c1f780f2b6d01be56021d4c6335bd673</citedby><cites>FETCH-LOGICAL-c451t-43b7df0bed94bce63e197dd4d5d11a02c1f780f2b6d01be56021d4c6335bd673</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.placenta.2009.04.009$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,778,782,3539,27907,27908,45978</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=21726596$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19467703$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Bryant-Greenwood, G.D</creatorcontrib><creatorcontrib>Yamamoto, S.Y</creatorcontrib><creatorcontrib>Sadowsky, D.W</creatorcontrib><creatorcontrib>Gravett, M.G</creatorcontrib><creatorcontrib>Novy, M.J</creatorcontrib><title>Relaxin Stimulates Interleukin-6 and Interleukin-8 Secretion from the Extraplacental Chorionic Cytotrophoblast</title><title>Placenta (Eastbourne)</title><addtitle>Placenta</addtitle><description>Abstract In the absence of infection, decidual relaxin (RLN) expression is increased in patients with preterm premature rupture of the membranes (PPROM) resulting in preterm birth, but it is not known whether inflammation stimulates RLN expression or vice versa. This study examined the effect of lipopolysaccharide (LPS) on the expression of RLN mRNA and secreted protein and whether RLN treatment influences secretion of proinflammatory cytokines from the fetal membranes. Explants of human fetal membranes in vitro and rhesus monkey fetal membranes in vivo were treated with LPS, which increased expression of IL-6 but had no effect on RLN. RLN treatment stimulated IL-6 and IL-8 secretion from choriodecidual explants in a subset of patients, as well as from isolated chorionic cytotrophoblast cells but not decidual cells. In vivo results obtained in rhesus monkeys after intra-amniotic infusion of RLN demonstrated increased IL-6 and IL-8 concentrations in amniotic fluid. Our results indicate that increased decidual RLN expression is independent of LPS but may induce a local sterile inflammatory process which potentially contributes to extracellular matrix degradation and weakening of the fetal membranes.</description><subject>Amniotic Fluid - metabolism</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cells, Cultured</subject><subject>Chorion - cytology</subject><subject>Chorion - drug effects</subject><subject>Chorion - secretion</subject><subject>Decidua - cytology</subject><subject>Decidua - drug effects</subject><subject>Decidua - secretion</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Extracellular Matrix - metabolism</subject><subject>Extraembryonic Membranes - cytology</subject><subject>Extraembryonic Membranes - drug effects</subject><subject>Extraembryonic Membranes - secretion</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression - drug effects</subject><subject>Gene Expression - physiology</subject><subject>Humans</subject><subject>Interleukin-6 - genetics</subject><subject>Interleukin-6 - secretion</subject><subject>Interleukin-8 - genetics</subject><subject>Interleukin-8 - secretion</subject><subject>Internal Medicine</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macaca mulatta</subject><subject>Obstetrics and Gynecology</subject><subject>Pregnancy</subject><subject>Preterm premature rupture of membranes</subject><subject>Proinflammatory cytokines</subject><subject>Recombinant Proteins - metabolism</subject><subject>Recombinant Proteins - pharmacology</subject><subject>Relaxin</subject><subject>Relaxin - genetics</subject><subject>Relaxin - metabolism</subject><subject>Relaxin - pharmacology</subject><subject>Trophoblasts - drug effects</subject><subject>Trophoblasts - secretion</subject><issn>0143-4004</issn><issn>1532-3102</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1v1DAQhiMEokvhL1S5wC1h_BGnuSDQqkClSkhs75ZjT7TeOvZiO6j77_FqtyC4cHol-5nx-NFU1RWBlgAR73ft3imNPquWAgwt8LbEs2pFOkYbRoA-r1ZAOGs4AL-oXqW0g0JwQl9WF2Tgou-BrSr_HZ16tL7eZDsvTmVM9a3PGB0uD9Y3olbe_HVyXW9QR8w2-HqKYa7zFuubxxzV00SuXm9DLPdW1-tDDjmG_TaMTqX8unoxKZfwzTkvq_vPN_frr83dty-36093jeYdyQ1nY28mGNEMfNQoGJKhN4abzhCigGoy9dcw0VEYICN2AigxXAvGutGInl1W705t9zH8WDBlOduk0TnlMSxJip4Xif1QQHECdQwpRZzkPtpZxYMkII-i5U4-fUseRUvgskQpvDq_sIwzmj9lZ7MFeHsGVNLKTVF5bdNvjpKeim4Qhft44rDo-GkxyqQteo3GRtRZmmD_P8uHf1poZ4t85R7wgGkXluiLbElkohLk5rgWx62AAYAwwtgvpay2Pw</recordid><startdate>20090701</startdate><enddate>20090701</enddate><creator>Bryant-Greenwood, G.D</creator><creator>Yamamoto, S.Y</creator><creator>Sadowsky, D.W</creator><creator>Gravett, M.G</creator><creator>Novy, M.J</creator><general>Elsevier Ltd</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20090701</creationdate><title>Relaxin Stimulates Interleukin-6 and Interleukin-8 Secretion from the Extraplacental Chorionic Cytotrophoblast</title><author>Bryant-Greenwood, G.D ; Yamamoto, S.Y ; Sadowsky, D.W ; Gravett, M.G ; Novy, M.J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-43b7df0bed94bce63e197dd4d5d11a02c1f780f2b6d01be56021d4c6335bd673</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Amniotic Fluid - metabolism</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Cells, Cultured</topic><topic>Chorion - cytology</topic><topic>Chorion - drug effects</topic><topic>Chorion - secretion</topic><topic>Decidua - cytology</topic><topic>Decidua - drug effects</topic><topic>Decidua - secretion</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Extracellular Matrix - metabolism</topic><topic>Extraembryonic Membranes - cytology</topic><topic>Extraembryonic Membranes - drug effects</topic><topic>Extraembryonic Membranes - secretion</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression - drug effects</topic><topic>Gene Expression - physiology</topic><topic>Humans</topic><topic>Interleukin-6 - genetics</topic><topic>Interleukin-6 - secretion</topic><topic>Interleukin-8 - genetics</topic><topic>Interleukin-8 - secretion</topic><topic>Internal Medicine</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macaca mulatta</topic><topic>Obstetrics and Gynecology</topic><topic>Pregnancy</topic><topic>Preterm premature rupture of membranes</topic><topic>Proinflammatory cytokines</topic><topic>Recombinant Proteins - metabolism</topic><topic>Recombinant Proteins - pharmacology</topic><topic>Relaxin</topic><topic>Relaxin - genetics</topic><topic>Relaxin - metabolism</topic><topic>Relaxin - pharmacology</topic><topic>Trophoblasts - drug effects</topic><topic>Trophoblasts - secretion</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Bryant-Greenwood, G.D</creatorcontrib><creatorcontrib>Yamamoto, S.Y</creatorcontrib><creatorcontrib>Sadowsky, D.W</creatorcontrib><creatorcontrib>Gravett, M.G</creatorcontrib><creatorcontrib>Novy, M.J</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Placenta (Eastbourne)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Bryant-Greenwood, G.D</au><au>Yamamoto, S.Y</au><au>Sadowsky, D.W</au><au>Gravett, M.G</au><au>Novy, M.J</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relaxin Stimulates Interleukin-6 and Interleukin-8 Secretion from the Extraplacental Chorionic Cytotrophoblast</atitle><jtitle>Placenta (Eastbourne)</jtitle><addtitle>Placenta</addtitle><date>2009-07-01</date><risdate>2009</risdate><volume>30</volume><issue>7</issue><spage>599</spage><epage>606</epage><pages>599-606</pages><issn>0143-4004</issn><eissn>1532-3102</eissn><coden>PLACDF</coden><abstract>Abstract In the absence of infection, decidual relaxin (RLN) expression is increased in patients with preterm premature rupture of the membranes (PPROM) resulting in preterm birth, but it is not known whether inflammation stimulates RLN expression or vice versa. This study examined the effect of lipopolysaccharide (LPS) on the expression of RLN mRNA and secreted protein and whether RLN treatment influences secretion of proinflammatory cytokines from the fetal membranes. Explants of human fetal membranes in vitro and rhesus monkey fetal membranes in vivo were treated with LPS, which increased expression of IL-6 but had no effect on RLN. RLN treatment stimulated IL-6 and IL-8 secretion from choriodecidual explants in a subset of patients, as well as from isolated chorionic cytotrophoblast cells but not decidual cells. In vivo results obtained in rhesus monkeys after intra-amniotic infusion of RLN demonstrated increased IL-6 and IL-8 concentrations in amniotic fluid. Our results indicate that increased decidual RLN expression is independent of LPS but may induce a local sterile inflammatory process which potentially contributes to extracellular matrix degradation and weakening of the fetal membranes.</abstract><cop>Kidlington</cop><pub>Elsevier Ltd</pub><pmid>19467703</pmid><doi>10.1016/j.placenta.2009.04.009</doi><tpages>8</tpages></addata></record> |
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| ispartof | Placenta (Eastbourne), 2009-07, Vol.30 (7), p.599-606 |
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| subjects | Amniotic Fluid - metabolism Animals Biological and medical sciences Cells, Cultured Chorion - cytology Chorion - drug effects Chorion - secretion Decidua - cytology Decidua - drug effects Decidua - secretion Embryology: invertebrates and vertebrates. Teratology Extracellular Matrix - metabolism Extraembryonic Membranes - cytology Extraembryonic Membranes - drug effects Extraembryonic Membranes - secretion Female Fundamental and applied biological sciences. Psychology Gene Expression - drug effects Gene Expression - physiology Humans Interleukin-6 - genetics Interleukin-6 - secretion Interleukin-8 - genetics Interleukin-8 - secretion Internal Medicine Lipopolysaccharides - pharmacology Macaca mulatta Obstetrics and Gynecology Pregnancy Preterm premature rupture of membranes Proinflammatory cytokines Recombinant Proteins - metabolism Recombinant Proteins - pharmacology Relaxin Relaxin - genetics Relaxin - metabolism Relaxin - pharmacology Trophoblasts - drug effects Trophoblasts - secretion |
| title | Relaxin Stimulates Interleukin-6 and Interleukin-8 Secretion from the Extraplacental Chorionic Cytotrophoblast |
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