Improved hollow-fibre membranes for dye-affinity chromatography
Hollow‐fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G‐A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity...
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| Veröffentlicht in: | Journal of separation science 2005-01, Vol.28 (1), p.45-51 |
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| container_title | Journal of separation science |
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| creator | Wolman, Federico J. Smolko, Eduardo E. Cascone, Osvaldo Grasselli, Mariano |
| description | Hollow‐fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G‐A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As the hydrophilicity increased the permeability decreased from 352 mL/cm2 min MPa for membranes grafted with GMA with ammonia spacer to 12.7 mL/cm2 min MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 ± 2.2 mg BSA/mL membrane and 58.3 ± 6.6 mg lysozyme/mL membrane while dissociation constants were 0.27 ± 0.16 and 0.13 ± 0.12 mg/mL, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0. |
| doi_str_mv | 10.1002/jssc.200401915 |
| format | Article |
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Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As the hydrophilicity increased the permeability decreased from 352 mL/cm2 min MPa for membranes grafted with GMA with ammonia spacer to 12.7 mL/cm2 min MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 ± 2.2 mg BSA/mL membrane and 58.3 ± 6.6 mg lysozyme/mL membrane while dissociation constants were 0.27 ± 0.16 and 0.13 ± 0.12 mg/mL, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0.</description><identifier>ISSN: 1615-9306</identifier><identifier>EISSN: 1615-9314</identifier><identifier>DOI: 10.1002/jssc.200401915</identifier><identifier>PMID: 15688630</identifier><language>eng</language><publisher>Weinheim: WILEY-VCH Verlag</publisher><subject>Acrylamides ; Adsorption ; Affinity chromatography ; Animals ; Cattle ; Chromatography, Affinity - methods ; Coloring Agents ; Dye ligands ; Epoxy Compounds ; Hollow-fibre membranes ; Hydrophobic and Hydrophilic Interactions ; Membranes, Artificial ; Methacrylates ; Muramidase - isolation & purification ; Permeability ; Polymers ; Proteins - isolation & purification ; Serum Albumin, Bovine - isolation & purification ; Surface Properties ; Triazines</subject><ispartof>Journal of separation science, 2005-01, Vol.28 (1), p.45-51</ispartof><rights>Copyright © 2005 WILEY‐VCH Verlag GmbH & Co. KGaA, Weinheim</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3815-7fb3c5c6d4fa14c26b0b849220c01c7796a009bcbec1d152803ebdab85af72833</citedby><cites>FETCH-LOGICAL-c3815-7fb3c5c6d4fa14c26b0b849220c01c7796a009bcbec1d152803ebdab85af72833</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjssc.200401915$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45575</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15688630$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Wolman, Federico J.</creatorcontrib><creatorcontrib>Smolko, Eduardo E.</creatorcontrib><creatorcontrib>Cascone, Osvaldo</creatorcontrib><creatorcontrib>Grasselli, Mariano</creatorcontrib><title>Improved hollow-fibre membranes for dye-affinity chromatography</title><title>Journal of separation science</title><addtitle>J. Sep. Science</addtitle><description>Hollow‐fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G‐A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As the hydrophilicity increased the permeability decreased from 352 mL/cm2 min MPa for membranes grafted with GMA with ammonia spacer to 12.7 mL/cm2 min MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 ± 2.2 mg BSA/mL membrane and 58.3 ± 6.6 mg lysozyme/mL membrane while dissociation constants were 0.27 ± 0.16 and 0.13 ± 0.12 mg/mL, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0.</description><subject>Acrylamides</subject><subject>Adsorption</subject><subject>Affinity chromatography</subject><subject>Animals</subject><subject>Cattle</subject><subject>Chromatography, Affinity - methods</subject><subject>Coloring Agents</subject><subject>Dye ligands</subject><subject>Epoxy Compounds</subject><subject>Hollow-fibre membranes</subject><subject>Hydrophobic and Hydrophilic Interactions</subject><subject>Membranes, Artificial</subject><subject>Methacrylates</subject><subject>Muramidase - isolation & purification</subject><subject>Permeability</subject><subject>Polymers</subject><subject>Proteins - isolation & purification</subject><subject>Serum Albumin, Bovine - isolation & purification</subject><subject>Surface Properties</subject><subject>Triazines</subject><issn>1615-9306</issn><issn>1615-9314</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM9PwjAYhhujEUSvHs1O3oZf161dT8YQQQzRGPxxbNquleHGsB3i_ntHIOjN0_cdnvfNkxehcwx9DBBdzb3X_QggBsxxcoC6mOIk5ATHh_sfaAedeD8HwCzlcIw6OKFpSgl00fW4XLrqy2TBrCqKah3aXDkTlKZUTi6MD2zlgqwxobQ2X-R1E-iZq0pZV-9OLmfNKTqysvDmbHd76GV4-zy4CyePo_HgZhJqkrYSzCqiE02z2Eoc64gqUGnMowg0YM0YpxKAK62MxhlOohSIUZlUaSIti1JCeuhy29vafq6Mr0WZe22KopWsVl5QRjiLSNKC_S2oXeW9M1YsXV5K1wgMYjOZ2Ewm9pO1gYtd80qVJvvFdxu1AN8C67wwzT914n46HfwtD7fZ3Nfme5-V7mNjzBLx9jAS7AkPh-x1IjD5ATbih-I</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Wolman, Federico J.</creator><creator>Smolko, Eduardo E.</creator><creator>Cascone, Osvaldo</creator><creator>Grasselli, Mariano</creator><general>WILEY-VCH Verlag</general><general>WILEY‐VCH Verlag</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Improved hollow-fibre membranes for dye-affinity chromatography</title><author>Wolman, Federico J. ; Smolko, Eduardo E. ; Cascone, Osvaldo ; Grasselli, Mariano</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3815-7fb3c5c6d4fa14c26b0b849220c01c7796a009bcbec1d152803ebdab85af72833</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acrylamides</topic><topic>Adsorption</topic><topic>Affinity chromatography</topic><topic>Animals</topic><topic>Cattle</topic><topic>Chromatography, Affinity - methods</topic><topic>Coloring Agents</topic><topic>Dye ligands</topic><topic>Epoxy Compounds</topic><topic>Hollow-fibre membranes</topic><topic>Hydrophobic and Hydrophilic Interactions</topic><topic>Membranes, Artificial</topic><topic>Methacrylates</topic><topic>Muramidase - isolation & purification</topic><topic>Permeability</topic><topic>Polymers</topic><topic>Proteins - isolation & purification</topic><topic>Serum Albumin, Bovine - isolation & purification</topic><topic>Surface Properties</topic><topic>Triazines</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wolman, Federico J.</creatorcontrib><creatorcontrib>Smolko, Eduardo E.</creatorcontrib><creatorcontrib>Cascone, Osvaldo</creatorcontrib><creatorcontrib>Grasselli, Mariano</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of separation science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wolman, Federico J.</au><au>Smolko, Eduardo E.</au><au>Cascone, Osvaldo</au><au>Grasselli, Mariano</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Improved hollow-fibre membranes for dye-affinity chromatography</atitle><jtitle>Journal of separation science</jtitle><addtitle>J. Sep. Science</addtitle><date>2005-01</date><risdate>2005</risdate><volume>28</volume><issue>1</issue><spage>45</spage><epage>51</epage><pages>45-51</pages><issn>1615-9306</issn><eissn>1615-9314</eissn><abstract>Hollow‐fibre membranes with different degrees of surface hydrophilicity were obtained by grafting mixtures of glycidyl methacrylate (GMA) and dimethyl acrylamide (DMAA) in various proportions, and Cibacron Blue F3G‐A was attached to them through ammonia or glucamine spacers. Membrane hydrophilicity increased with the amount of dimethyl acrylamide in the grafted polymer. As the hydrophilicity increased the permeability decreased from 352 mL/cm2 min MPa for membranes grafted with GMA with ammonia spacer to 12.7 mL/cm2 min MPa for membranes grafted with GMA/DMAA 1/3 with glucamine spacer. Membranes grafted with GMA/DMAA 1/3 with ammonia spacer showed the best performance for BSA and lysozyme adsorption: maximum capacity was 15.3 ± 2.2 mg BSA/mL membrane and 58.3 ± 6.6 mg lysozyme/mL membrane while dissociation constants were 0.27 ± 0.16 and 0.13 ± 0.12 mg/mL, respectively. Over 80% of adsorbed proteins could be eluted with 2 M NaCl + 20% isopropanol in 20 mM sodium phosphate buffer, pH 7.0.</abstract><cop>Weinheim</cop><pub>WILEY-VCH Verlag</pub><pmid>15688630</pmid><doi>10.1002/jssc.200401915</doi><tpages>7</tpages></addata></record> |
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| subjects | Acrylamides Adsorption Affinity chromatography Animals Cattle Chromatography, Affinity - methods Coloring Agents Dye ligands Epoxy Compounds Hollow-fibre membranes Hydrophobic and Hydrophilic Interactions Membranes, Artificial Methacrylates Muramidase - isolation & purification Permeability Polymers Proteins - isolation & purification Serum Albumin, Bovine - isolation & purification Surface Properties Triazines |
| title | Improved hollow-fibre membranes for dye-affinity chromatography |
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