Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)
Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highes...
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| Veröffentlicht in: | Biological & pharmaceutical bulletin 2005, Vol.28(2), pp.299-304 |
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| container_title | Biological & pharmaceutical bulletin |
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| creator | Sakano, Yuichi Mutsuga, Motoh Tanaka, Rie Suganuma, Hiroyuki Inakuma, Takahiro Toyoda, Masatake Goda, Yukihiro Shibuya, Masaaki Ebizuka, Yutaka |
| description | Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 μg/ml). Examination of activity variation among eight taro cultivars indicated that “Aichi-wase” and “Yatsugashira” had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of “Aichi-wase” were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300 μg/ml. |
| doi_str_mv | 10.1248/bpb.28.299 |
| format | Article |
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Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 μg/ml). Examination of activity variation among eight taro cultivars indicated that “Aichi-wase” and “Yatsugashira” had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of “Aichi-wase” were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300 μg/ml.</description><identifier>ISSN: 0918-6158</identifier><identifier>EISSN: 1347-5215</identifier><identifier>DOI: 10.1248/bpb.28.299</identifier><identifier>PMID: 15684488</identifier><language>eng</language><publisher>Japan: The Pharmaceutical Society of Japan</publisher><subject>cholesterol biosynthesis ; Colocasia ; Colocasia esculenta ; digalactosyldiacylglycerol ; Enzyme Inhibitors - isolation & purification ; Enzyme Inhibitors - pharmacology ; Humans ; Intramolecular Transferases - antagonists & inhibitors ; Intramolecular Transferases - metabolism ; lanosterol synthase ; monogalactosyldiacylglycerol ; Plant Extracts - isolation & purification ; Plant Extracts - pharmacology</subject><ispartof>Biological and Pharmaceutical Bulletin, 2005, Vol.28(2), pp.299-304</ispartof><rights>2005 The Pharmaceutical Society of Japan</rights><rights>Copyright Japan Science and Technology Agency 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c621t-f89ea205e30edb17e47d0692df7321ba4c7f9fbd26fa0267e71a40096511eecf3</citedby><cites>FETCH-LOGICAL-c621t-f89ea205e30edb17e47d0692df7321ba4c7f9fbd26fa0267e71a40096511eecf3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,1877,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15684488$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Sakano, Yuichi</creatorcontrib><creatorcontrib>Mutsuga, Motoh</creatorcontrib><creatorcontrib>Tanaka, Rie</creatorcontrib><creatorcontrib>Suganuma, Hiroyuki</creatorcontrib><creatorcontrib>Inakuma, Takahiro</creatorcontrib><creatorcontrib>Toyoda, Masatake</creatorcontrib><creatorcontrib>Goda, Yukihiro</creatorcontrib><creatorcontrib>Shibuya, Masaaki</creatorcontrib><creatorcontrib>Ebizuka, Yutaka</creatorcontrib><title>Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)</title><title>Biological & pharmaceutical bulletin</title><addtitle>Biol Pharm Bull</addtitle><description>Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 μg/ml). Examination of activity variation among eight taro cultivars indicated that “Aichi-wase” and “Yatsugashira” had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of “Aichi-wase” were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. Further purification of these fractions by preparative HPLC gave three monogalactosyldiacylglycerols and five digalactosyldiacylglycerols as active compounds that showed 28 to 67% inhibitory activities at the concentration 300 μg/ml.</description><subject>cholesterol biosynthesis</subject><subject>Colocasia</subject><subject>Colocasia esculenta</subject><subject>digalactosyldiacylglycerol</subject><subject>Enzyme Inhibitors - isolation & purification</subject><subject>Enzyme Inhibitors - pharmacology</subject><subject>Humans</subject><subject>Intramolecular Transferases - antagonists & inhibitors</subject><subject>Intramolecular Transferases - metabolism</subject><subject>lanosterol synthase</subject><subject>monogalactosyldiacylglycerol</subject><subject>Plant Extracts - isolation & purification</subject><subject>Plant Extracts - pharmacology</subject><issn>0918-6158</issn><issn>1347-5215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpd0MtqGzEUBmBRWho37aYPUASF0hbG0W10WYVikiZg6CLpIiuh0RzVY8aSK2kWfvtMsHGhGwmk7_wcfoQ-UrKkTOirbt8tmV4yY16hBeVCNS2j7Wu0IIbqRtJWX6B3pWwJIYow_hZd0FZqIbReoKf7uBm6oQ4p4hTw3bRzEa9dTKVCTiN-OMS6cQVwd8B1A3iVYqlDnSDW8jKwSmPyrgwOQ_HTOD87_PXR5fTtPXoT3Fjgw-m-RL9vbx5Xd83618_71Y914yWjtQnagGOkBU6g76gCoXoiDeuD4ox2TngVTOh6JoMjTCpQ1AlCjGwpBfCBX6Ivx9x9Tn8nKNXuhuJhHF2ENBUrFTfSUDXDz__BbZpynHezVAjDudSEzur7UfmcSskQ7D4PO5cPlhL7Ured67ZM27nuGX86RU7dDvp_9NTvDK6PYFuq-wNn4HId_AjnrNNhzPnHb1y2EPkzd7GRtQ</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Sakano, Yuichi</creator><creator>Mutsuga, Motoh</creator><creator>Tanaka, Rie</creator><creator>Suganuma, Hiroyuki</creator><creator>Inakuma, Takahiro</creator><creator>Toyoda, Masatake</creator><creator>Goda, Yukihiro</creator><creator>Shibuya, Masaaki</creator><creator>Ebizuka, Yutaka</creator><general>The Pharmaceutical Society of Japan</general><general>Japan Science and Technology Agency</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)</title><author>Sakano, Yuichi ; Mutsuga, Motoh ; Tanaka, Rie ; Suganuma, Hiroyuki ; Inakuma, Takahiro ; Toyoda, Masatake ; Goda, Yukihiro ; Shibuya, Masaaki ; Ebizuka, Yutaka</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c621t-f89ea205e30edb17e47d0692df7321ba4c7f9fbd26fa0267e71a40096511eecf3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>cholesterol biosynthesis</topic><topic>Colocasia</topic><topic>Colocasia esculenta</topic><topic>digalactosyldiacylglycerol</topic><topic>Enzyme Inhibitors - isolation & purification</topic><topic>Enzyme Inhibitors - pharmacology</topic><topic>Humans</topic><topic>Intramolecular Transferases - antagonists & inhibitors</topic><topic>Intramolecular Transferases - metabolism</topic><topic>lanosterol synthase</topic><topic>monogalactosyldiacylglycerol</topic><topic>Plant Extracts - isolation & purification</topic><topic>Plant Extracts - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Sakano, Yuichi</creatorcontrib><creatorcontrib>Mutsuga, Motoh</creatorcontrib><creatorcontrib>Tanaka, Rie</creatorcontrib><creatorcontrib>Suganuma, Hiroyuki</creatorcontrib><creatorcontrib>Inakuma, Takahiro</creatorcontrib><creatorcontrib>Toyoda, Masatake</creatorcontrib><creatorcontrib>Goda, Yukihiro</creatorcontrib><creatorcontrib>Shibuya, Masaaki</creatorcontrib><creatorcontrib>Ebizuka, Yutaka</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Biological & pharmaceutical bulletin</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Sakano, Yuichi</au><au>Mutsuga, Motoh</au><au>Tanaka, Rie</au><au>Suganuma, Hiroyuki</au><au>Inakuma, Takahiro</au><au>Toyoda, Masatake</au><au>Goda, Yukihiro</au><au>Shibuya, Masaaki</au><au>Ebizuka, Yutaka</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro)</atitle><jtitle>Biological & pharmaceutical bulletin</jtitle><addtitle>Biol Pharm Bull</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>28</volume><issue>2</issue><spage>299</spage><epage>304</epage><pages>299-304</pages><issn>0918-6158</issn><eissn>1347-5215</eissn><abstract>Ethanol extracts of lyophilized vegetables were tested for inhibition of human lanosterol synthase (hOSC) in order to find the compounds to suppress cholesterol biosynthesis. Of 130 samples tested, twelve samples showed significant inhibition. Among them, Colocasia esculenta (taro) showed the highest inhibition (55% inhibition at 300 μg/ml). Examination of activity variation among eight taro cultivars indicated that “Aichi-wase” and “Yatsugashira” had the most potent activity for hOSC inhibition. In order to identify the active constituent of taro, ethanol extracts of “Aichi-wase” were partitioned with hexane and aqueous methanol, and fractionated by silica gel column chromatography. Inhibitory activity was concentrated in two major active fractions. 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| subjects | cholesterol biosynthesis Colocasia Colocasia esculenta digalactosyldiacylglycerol Enzyme Inhibitors - isolation & purification Enzyme Inhibitors - pharmacology Humans Intramolecular Transferases - antagonists & inhibitors Intramolecular Transferases - metabolism lanosterol synthase monogalactosyldiacylglycerol Plant Extracts - isolation & purification Plant Extracts - pharmacology |
| title | Inhibition of Human Lanosterol Synthase by the Constituents of Colocasia esculenta (Taro) |
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