Characterization of a novel pestivirus originating from a pronghorn antelope
A unique pestivirus, isolated from a pronghorn antelope ( Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the Pestivirus...
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| Veröffentlicht in: | Virus research 2005-03, Vol.108 (1), p.187-193 |
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| creator | Vilcek, S. Ridpath, J.F. Van Campen, H. Cavender, J.L. Warg, J. |
| description | A unique pestivirus, isolated from a pronghorn antelope (
Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the
Pestivirus genus. These characteristics included the organization of the 5′ untranslated region (5′-UTR), the presence and length of a viral N
pro coding region, conservation of cysteine residues in N
pro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides N
pro and C and between C and E
rns and conservation of predicted hydrophobicity plots of N
pro protein. While this data indicated the virus belongs to the
Pestivirus genus, phylogenetic analysis in 5′-UTR, N
pro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife. |
| doi_str_mv | 10.1016/j.virusres.2004.09.010 |
| format | Article |
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Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the
Pestivirus genus. These characteristics included the organization of the 5′ untranslated region (5′-UTR), the presence and length of a viral N
pro coding region, conservation of cysteine residues in N
pro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides N
pro and C and between C and E
rns and conservation of predicted hydrophobicity plots of N
pro protein. While this data indicated the virus belongs to the
Pestivirus genus, phylogenetic analysis in 5′-UTR, N
pro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife.</description><identifier>ISSN: 0168-1702</identifier><identifier>EISSN: 1872-7492</identifier><identifier>DOI: 10.1016/j.virusres.2004.09.010</identifier><identifier>PMID: 15681069</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>5' Untranslated Regions - genetics ; Amino Acid Sequence ; Animals ; Antelopes - virology ; Antilocopra americana ; Base Sequence ; Conserved Sequence ; Genotype ; Molecular Sequence Data ; Neutralization Tests ; Pestivirus ; Pestivirus - classification ; Pestivirus - genetics ; Pestivirus - isolation & purification ; Pestivirus Infections - veterinary ; Pestivirus Infections - virology ; Phylogeny ; Pronghorn antelope ; Protein Processing, Post-Translational ; Sequence Homology ; Viral Proteins - chemistry ; Viral Proteins - genetics</subject><ispartof>Virus research, 2005-03, Vol.108 (1), p.187-193</ispartof><rights>2004 Elsevier B.V.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c463t-90448b2d4e73988c151ae46bac685b6b6a157a79bfaaf96f451d3b11e2452e683</citedby><cites>FETCH-LOGICAL-c463t-90448b2d4e73988c151ae46bac685b6b6a157a79bfaaf96f451d3b11e2452e683</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.virusres.2004.09.010$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,782,786,3554,27933,27934,46004</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15681069$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vilcek, S.</creatorcontrib><creatorcontrib>Ridpath, J.F.</creatorcontrib><creatorcontrib>Van Campen, H.</creatorcontrib><creatorcontrib>Cavender, J.L.</creatorcontrib><creatorcontrib>Warg, J.</creatorcontrib><title>Characterization of a novel pestivirus originating from a pronghorn antelope</title><title>Virus research</title><addtitle>Virus Res</addtitle><description>A unique pestivirus, isolated from a pronghorn antelope (
Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the
Pestivirus genus. These characteristics included the organization of the 5′ untranslated region (5′-UTR), the presence and length of a viral N
pro coding region, conservation of cysteine residues in N
pro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides N
pro and C and between C and E
rns and conservation of predicted hydrophobicity plots of N
pro protein. While this data indicated the virus belongs to the
Pestivirus genus, phylogenetic analysis in 5′-UTR, N
pro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife.</description><subject>5' Untranslated Regions - genetics</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Antelopes - virology</subject><subject>Antilocopra americana</subject><subject>Base Sequence</subject><subject>Conserved Sequence</subject><subject>Genotype</subject><subject>Molecular Sequence Data</subject><subject>Neutralization Tests</subject><subject>Pestivirus</subject><subject>Pestivirus - classification</subject><subject>Pestivirus - genetics</subject><subject>Pestivirus - isolation & purification</subject><subject>Pestivirus Infections - veterinary</subject><subject>Pestivirus Infections - virology</subject><subject>Phylogeny</subject><subject>Pronghorn antelope</subject><subject>Protein Processing, Post-Translational</subject><subject>Sequence Homology</subject><subject>Viral Proteins - chemistry</subject><subject>Viral Proteins - genetics</subject><issn>0168-1702</issn><issn>1872-7492</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkE1PGzEURa2qVQm0fwHNqrsZ_Gb8uaOKWkCKxIauLY_nTXA0sYM9iQS_HtMEsWT1Nufde3UIuQTaAAVxtWkOPu1zwty0lLKG6oYC_UIWoGRbS6bbr2RRQFWDpO0ZOc95QykVnRTfyRlwoYAKvSCr5aNN1s2Y_IudfQxVHCtbhXjAqdphnv3_niomv_ahEGFdjSluC7NLMawfYwqVDTNOcYc_yLfRThl_nu4F-ff3z8Pytl7d39wtf69qx0Q315oypvp2YCg7rZQDDhaZ6K0TiveiFxa4tFL3o7WjFiPjMHQ9ALaMtyhUd0F-HXPLhKd9GWm2PjucJhsw7rMRJZdz1n0KglQgudIFFEfQpZiL1dHskt_a9GyAmjfhZmPehZs34YZqU4SXx8tTw77f4vDxdjJcgOsjgEXIwWMy2XkMDgef0M1miP6zjlcTxZZj</recordid><startdate>20050301</startdate><enddate>20050301</enddate><creator>Vilcek, S.</creator><creator>Ridpath, J.F.</creator><creator>Van Campen, H.</creator><creator>Cavender, J.L.</creator><creator>Warg, J.</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope></search><sort><creationdate>20050301</creationdate><title>Characterization of a novel pestivirus originating from a pronghorn antelope</title><author>Vilcek, S. ; Ridpath, J.F. ; Van Campen, H. ; Cavender, J.L. ; Warg, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c463t-90448b2d4e73988c151ae46bac685b6b6a157a79bfaaf96f451d3b11e2452e683</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>5' Untranslated Regions - genetics</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Antelopes - virology</topic><topic>Antilocopra americana</topic><topic>Base Sequence</topic><topic>Conserved Sequence</topic><topic>Genotype</topic><topic>Molecular Sequence Data</topic><topic>Neutralization Tests</topic><topic>Pestivirus</topic><topic>Pestivirus - classification</topic><topic>Pestivirus - genetics</topic><topic>Pestivirus - isolation & purification</topic><topic>Pestivirus Infections - veterinary</topic><topic>Pestivirus Infections - virology</topic><topic>Phylogeny</topic><topic>Pronghorn antelope</topic><topic>Protein Processing, Post-Translational</topic><topic>Sequence Homology</topic><topic>Viral Proteins - chemistry</topic><topic>Viral Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vilcek, S.</creatorcontrib><creatorcontrib>Ridpath, J.F.</creatorcontrib><creatorcontrib>Van Campen, H.</creatorcontrib><creatorcontrib>Cavender, J.L.</creatorcontrib><creatorcontrib>Warg, J.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Virus research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vilcek, S.</au><au>Ridpath, J.F.</au><au>Van Campen, H.</au><au>Cavender, J.L.</au><au>Warg, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a novel pestivirus originating from a pronghorn antelope</atitle><jtitle>Virus research</jtitle><addtitle>Virus Res</addtitle><date>2005-03-01</date><risdate>2005</risdate><volume>108</volume><issue>1</issue><spage>187</spage><epage>193</epage><pages>187-193</pages><issn>0168-1702</issn><eissn>1872-7492</eissn><abstract>A unique pestivirus, isolated from a pronghorn antelope (
Antilocopra americana), was characterized. Serum neutralization studies suggested that this virus was antigenically related to pestiviruses. Genomic characteristics, unique to pestiviruses, indicated that this virus belongs to the
Pestivirus genus. These characteristics included the organization of the 5′ untranslated region (5′-UTR), the presence and length of a viral N
pro coding region, conservation of cysteine residues in N
pro, conservation of predicted amino acid sequences flanking the cleavage sites between viral polypeptides N
pro and C and between C and E
rns and conservation of predicted hydrophobicity plots of N
pro protein. While this data indicated the virus belongs to the
Pestivirus genus, phylogenetic analysis in 5′-UTR, N
pro and E2 regions suggested that it is the most divergent of the pestiviruses identified to date. This conclusion was also supported by the amino acid identity in coding regions. The corresponding values were much lower for the comparison of pronghorn pestivirus to other pestivirus genotypes than only between previous recognized genotypes. These results suggest the virus isolated from pronghorn antelope represents a new pestivirus genotype. It also represents the only pestivirus genotype first isolated from New World wildlife.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15681069</pmid><doi>10.1016/j.virusres.2004.09.010</doi><tpages>7</tpages></addata></record> |
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| ispartof | Virus research, 2005-03, Vol.108 (1), p.187-193 |
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| source | MEDLINE; Access via ScienceDirect (Elsevier) |
| subjects | 5' Untranslated Regions - genetics Amino Acid Sequence Animals Antelopes - virology Antilocopra americana Base Sequence Conserved Sequence Genotype Molecular Sequence Data Neutralization Tests Pestivirus Pestivirus - classification Pestivirus - genetics Pestivirus - isolation & purification Pestivirus Infections - veterinary Pestivirus Infections - virology Phylogeny Pronghorn antelope Protein Processing, Post-Translational Sequence Homology Viral Proteins - chemistry Viral Proteins - genetics |
| title | Characterization of a novel pestivirus originating from a pronghorn antelope |
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