Mouse Strain–Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease
OBJECTIVE—Different strains of inbred mice exhibit different susceptibility to the development of atherosclerosis. The C3H/HeJ and C57Bl/6 mice have been used in several studies aimed at understanding the genetic basis of atherosclerosis. Under controlled environmental conditions, variations in susc...
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| Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2005-02, Vol.25 (2), p.302-308 |
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| container_title | Arteriosclerosis, thrombosis, and vascular biology |
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| creator | Tabibiazar, Raymond Wagner, Roger A Spin, Joshua M Ashley, Euan A Narasimhan, Balasubramanian Rubin, Edward M Efron, Bradley Tsao, Phil S Tibshirani, Robert Quertermous, Thomas |
| description | OBJECTIVE—Different strains of inbred mice exhibit different susceptibility to the development of atherosclerosis. The C3H/HeJ and C57Bl/6 mice have been used in several studies aimed at understanding the genetic basis of atherosclerosis. Under controlled environmental conditions, variations in susceptibility to atherosclerosis reflect differences in genetic makeup, and these differences must be reflected in gene expression patterns that are temporally related to the development of disease. In this study, we sought to identify the genetic pathways that are differentially activated in the aortas of these mice.
METHODS AND RESULTS—We performed genome-wide transcriptional profiling of aortas from C3H/HeJ and C57Bl/6 mice. Differences in gene expression were identified at baseline as well as during normal aging and longitudinal exposure to high-fat diet. The significance of these genes to the development of atherosclerosis was evaluated by observing their temporal pattern of expression in the well-studied apolipoprotein E model of atherosclerosis.
CONCLUSION—Gene expression differences between the 2 strains suggest that aortas of C57Bl/6 mice have a higher genetic propensity to develop inflammation in response to appropriate atherogenic stimuli. This study expands the repertoire of factors in known disease-related signaling pathways and identifies novel candidate genes for future study. |
| doi_str_mv | 10.1161/01.ATV.0000151372.86863.a5 |
| format | Article |
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METHODS AND RESULTS—We performed genome-wide transcriptional profiling of aortas from C3H/HeJ and C57Bl/6 mice. Differences in gene expression were identified at baseline as well as during normal aging and longitudinal exposure to high-fat diet. The significance of these genes to the development of atherosclerosis was evaluated by observing their temporal pattern of expression in the well-studied apolipoprotein E model of atherosclerosis.
CONCLUSION—Gene expression differences between the 2 strains suggest that aortas of C57Bl/6 mice have a higher genetic propensity to develop inflammation in response to appropriate atherogenic stimuli. This study expands the repertoire of factors in known disease-related signaling pathways and identifies novel candidate genes for future study.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.ATV.0000151372.86863.a5</identifier><identifier>PMID: 15550693</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Aging - genetics ; Aging - metabolism ; Animals ; Aorta - metabolism ; Aorta - pathology ; Aortitis - genetics ; Aortitis - metabolism ; Aortitis - pathology ; Apolipoproteins E - deficiency ; Apolipoproteins E - genetics ; Arteriosclerosis - genetics ; Arteriosclerosis - metabolism ; Arteriosclerosis - pathology ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood vessels and receptors ; Cardiology. Vascular system ; Diet, Atherogenic ; Dietary Fats - pharmacology ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Female ; Fundamental and applied biological sciences. Psychology ; Gene Expression Profiling ; Gene Expression Regulation, Developmental ; Genetic Predisposition to Disease ; Inflammation - genetics ; Medical sciences ; Mice ; Mice, Inbred C3H - genetics ; Mice, Inbred C3H - metabolism ; Mice, Inbred C57BL - genetics ; Mice, Inbred C57BL - metabolism ; Mice, Knockout ; Oligonucleotide Array Sequence Analysis ; Reverse Transcriptase Polymerase Chain Reaction ; Transcription, Genetic ; Vertebrates: cardiovascular system</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2005-02, Vol.25 (2), p.302-308</ispartof><rights>2005 American Heart Association, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Feb 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4521-319c8d92c6cd8b3cae5f1f3b163186adeef0e3aa47c1e0652596743cca00cdc3</citedby><cites>FETCH-LOGICAL-c4521-319c8d92c6cd8b3cae5f1f3b163186adeef0e3aa47c1e0652596743cca00cdc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16514772$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15550693$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tabibiazar, Raymond</creatorcontrib><creatorcontrib>Wagner, Roger A</creatorcontrib><creatorcontrib>Spin, Joshua M</creatorcontrib><creatorcontrib>Ashley, Euan A</creatorcontrib><creatorcontrib>Narasimhan, Balasubramanian</creatorcontrib><creatorcontrib>Rubin, Edward M</creatorcontrib><creatorcontrib>Efron, Bradley</creatorcontrib><creatorcontrib>Tsao, Phil S</creatorcontrib><creatorcontrib>Tibshirani, Robert</creatorcontrib><creatorcontrib>Quertermous, Thomas</creatorcontrib><title>Mouse Strain–Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—Different strains of inbred mice exhibit different susceptibility to the development of atherosclerosis. The C3H/HeJ and C57Bl/6 mice have been used in several studies aimed at understanding the genetic basis of atherosclerosis. Under controlled environmental conditions, variations in susceptibility to atherosclerosis reflect differences in genetic makeup, and these differences must be reflected in gene expression patterns that are temporally related to the development of disease. In this study, we sought to identify the genetic pathways that are differentially activated in the aortas of these mice.
METHODS AND RESULTS—We performed genome-wide transcriptional profiling of aortas from C3H/HeJ and C57Bl/6 mice. Differences in gene expression were identified at baseline as well as during normal aging and longitudinal exposure to high-fat diet. The significance of these genes to the development of atherosclerosis was evaluated by observing their temporal pattern of expression in the well-studied apolipoprotein E model of atherosclerosis.
CONCLUSION—Gene expression differences between the 2 strains suggest that aortas of C57Bl/6 mice have a higher genetic propensity to develop inflammation in response to appropriate atherogenic stimuli. This study expands the repertoire of factors in known disease-related signaling pathways and identifies novel candidate genes for future study.</description><subject>Aging - genetics</subject><subject>Aging - metabolism</subject><subject>Animals</subject><subject>Aorta - metabolism</subject><subject>Aorta - pathology</subject><subject>Aortitis - genetics</subject><subject>Aortitis - metabolism</subject><subject>Aortitis - pathology</subject><subject>Apolipoproteins E - deficiency</subject><subject>Apolipoproteins E - genetics</subject><subject>Arteriosclerosis - genetics</subject><subject>Arteriosclerosis - metabolism</subject><subject>Arteriosclerosis - pathology</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood vessels and receptors</subject><subject>Cardiology. Vascular system</subject><subject>Diet, Atherogenic</subject><subject>Dietary Fats - pharmacology</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Expression Profiling</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Genetic Predisposition to Disease</subject><subject>Inflammation - genetics</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H - genetics</subject><subject>Mice, Inbred C3H - metabolism</subject><subject>Mice, Inbred C57BL - genetics</subject><subject>Mice, Inbred C57BL - metabolism</subject><subject>Mice, Knockout</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Transcription, Genetic</subject><subject>Vertebrates: cardiovascular system</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkdtu1DAQhiMEoqXwCsiqRO8SPD4l4a7qCaQiJLoql9asM9G6eJPFTlS46zvwhjwJXnallfDF2Bp9_8x4_qI4BV4BGHjPoTpf3Fc8H9Aga1E1pjGyQv2sOAYtVKmMNM_zm9dtqY0SR8WrlB4yr4TgL4sj0Fpz08rjYvw8zonY3RTRD3-eft9tyPneO3bp-54iDY4S8wO7x-TmgJF9wxDYDQ3Ern5uIqXkx4Hh0LHFinxkXynglFNp5TdsGg-6S58IE70uXvQYEr3Z3yfF4vpqcfGxvP1y8-ni_LZ0SgsoJbSu6VrhjOuapXRIuodeLsFIaAx2RD0niahqB8SNFro1tZLOIeeuc_KkONuV3cTxx0xpsmufHIWAA-UPW1PLpjG1yeDpf-DDOMchj2ZF3lbTqkZm6MMOcnFMKVJvN9GvMf6ywO3WEsvBZkvswRL7zxKLOovf7jvMyzV1B-negwy82wN5Vxj6iIPz6cAZDaquRebUjnscw0QxfQ_zI0W7IgzTattaScN1KTjXPAdebocB-Rc4ZKYZ</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Tabibiazar, Raymond</creator><creator>Wagner, Roger A</creator><creator>Spin, Joshua M</creator><creator>Ashley, Euan A</creator><creator>Narasimhan, Balasubramanian</creator><creator>Rubin, Edward M</creator><creator>Efron, Bradley</creator><creator>Tsao, Phil S</creator><creator>Tibshirani, Robert</creator><creator>Quertermous, Thomas</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Mouse Strain–Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease</title><author>Tabibiazar, Raymond ; Wagner, Roger A ; Spin, Joshua M ; Ashley, Euan A ; Narasimhan, Balasubramanian ; Rubin, Edward M ; Efron, Bradley ; Tsao, Phil S ; Tibshirani, Robert ; Quertermous, Thomas</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4521-319c8d92c6cd8b3cae5f1f3b163186adeef0e3aa47c1e0652596743cca00cdc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aging - genetics</topic><topic>Aging - metabolism</topic><topic>Animals</topic><topic>Aorta - metabolism</topic><topic>Aorta - pathology</topic><topic>Aortitis - genetics</topic><topic>Aortitis - metabolism</topic><topic>Aortitis - pathology</topic><topic>Apolipoproteins E - deficiency</topic><topic>Apolipoproteins E - genetics</topic><topic>Arteriosclerosis - genetics</topic><topic>Arteriosclerosis - metabolism</topic><topic>Arteriosclerosis - pathology</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood vessels and receptors</topic><topic>Cardiology. Vascular system</topic><topic>Diet, Atherogenic</topic><topic>Dietary Fats - pharmacology</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Expression Profiling</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Genetic Predisposition to Disease</topic><topic>Inflammation - genetics</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H - genetics</topic><topic>Mice, Inbred C3H - metabolism</topic><topic>Mice, Inbred C57BL - genetics</topic><topic>Mice, Inbred C57BL - metabolism</topic><topic>Mice, Knockout</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>Transcription, Genetic</topic><topic>Vertebrates: cardiovascular system</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tabibiazar, Raymond</creatorcontrib><creatorcontrib>Wagner, Roger A</creatorcontrib><creatorcontrib>Spin, Joshua M</creatorcontrib><creatorcontrib>Ashley, Euan A</creatorcontrib><creatorcontrib>Narasimhan, Balasubramanian</creatorcontrib><creatorcontrib>Rubin, Edward M</creatorcontrib><creatorcontrib>Efron, Bradley</creatorcontrib><creatorcontrib>Tsao, Phil S</creatorcontrib><creatorcontrib>Tibshirani, Robert</creatorcontrib><creatorcontrib>Quertermous, Thomas</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tabibiazar, Raymond</au><au>Wagner, Roger A</au><au>Spin, Joshua M</au><au>Ashley, Euan A</au><au>Narasimhan, Balasubramanian</au><au>Rubin, Edward M</au><au>Efron, Bradley</au><au>Tsao, Phil S</au><au>Tibshirani, Robert</au><au>Quertermous, Thomas</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Mouse Strain–Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2005-02</date><risdate>2005</risdate><volume>25</volume><issue>2</issue><spage>302</spage><epage>308</epage><pages>302-308</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—Different strains of inbred mice exhibit different susceptibility to the development of atherosclerosis. The C3H/HeJ and C57Bl/6 mice have been used in several studies aimed at understanding the genetic basis of atherosclerosis. Under controlled environmental conditions, variations in susceptibility to atherosclerosis reflect differences in genetic makeup, and these differences must be reflected in gene expression patterns that are temporally related to the development of disease. In this study, we sought to identify the genetic pathways that are differentially activated in the aortas of these mice.
METHODS AND RESULTS—We performed genome-wide transcriptional profiling of aortas from C3H/HeJ and C57Bl/6 mice. Differences in gene expression were identified at baseline as well as during normal aging and longitudinal exposure to high-fat diet. The significance of these genes to the development of atherosclerosis was evaluated by observing their temporal pattern of expression in the well-studied apolipoprotein E model of atherosclerosis.
CONCLUSION—Gene expression differences between the 2 strains suggest that aortas of C57Bl/6 mice have a higher genetic propensity to develop inflammation in response to appropriate atherogenic stimuli. This study expands the repertoire of factors in known disease-related signaling pathways and identifies novel candidate genes for future study.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>15550693</pmid><doi>10.1161/01.ATV.0000151372.86863.a5</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1079-5642 |
| ispartof | Arteriosclerosis, thrombosis, and vascular biology, 2005-02, Vol.25 (2), p.302-308 |
| issn | 1079-5642 1524-4636 |
| language | eng |
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| subjects | Aging - genetics Aging - metabolism Animals Aorta - metabolism Aorta - pathology Aortitis - genetics Aortitis - metabolism Aortitis - pathology Apolipoproteins E - deficiency Apolipoproteins E - genetics Arteriosclerosis - genetics Arteriosclerosis - metabolism Arteriosclerosis - pathology Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood vessels and receptors Cardiology. Vascular system Diet, Atherogenic Dietary Fats - pharmacology Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Female Fundamental and applied biological sciences. Psychology Gene Expression Profiling Gene Expression Regulation, Developmental Genetic Predisposition to Disease Inflammation - genetics Medical sciences Mice Mice, Inbred C3H - genetics Mice, Inbred C3H - metabolism Mice, Inbred C57BL - genetics Mice, Inbred C57BL - metabolism Mice, Knockout Oligonucleotide Array Sequence Analysis Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic Vertebrates: cardiovascular system |
| title | Mouse Strain–Specific Differences in Vascular Wall Gene Expression and Their Relationship to Vascular Disease |
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