Prostaglandin E Synthases in Zebrafish
OBJECTIVE—Prostaglandin E synthases (PGESs) are being explored as antiinflammtory drug targets as alternatives to cyclooxygenase (COX)-2. Located downstream of the cyclooxygenases, PGESs catalyze PGE2 formation, and deletion of microsomal (m)-PGES-1 abrogates inflammation. We sought to characterize...
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Veröffentlicht in: | Arteriosclerosis, thrombosis, and vascular biology thrombosis, and vascular biology, 2005-02, Vol.25 (2), p.315-320 |
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creator | Pini, Barbara Grosser, Tilo Lawson, John A Price, Tom S Pack, Michael A FitzGerald, Garret A |
description | OBJECTIVE—Prostaglandin E synthases (PGESs) are being explored as antiinflammtory drug targets as alternatives to cyclooxygenase (COX)-2. Located downstream of the cyclooxygenases, PGESs catalyze PGE2 formation, and deletion of microsomal (m)-PGES-1 abrogates inflammation. We sought to characterize the developmental expression of COX and PGES in zebrafish.
METHODS AND RESULTS—We cloned zebrafish cytosolic (c) and m-PGES orthologs and mapped them to syntenic regions of chromosomes 23 and 5. cPGES was widely expressed during development and was coordinately regulated with zCOX-1 in the inner ear, the pronephros, and intestine. COX-2 and mPGES-1 exhibited restricted expression, dominantly in the vasculature of the aortic arch. However, the enzymes were anatomically segregated within the vessel wall. Experiments with antisense morpholinos and with nonsteroidal antiinflammatory drugs suggest that these genes may not be critical for development.
CONCLUSIONS—mPGES-1 is developmentally coregulated with COX-2 in vasculature. Given the high fecundidity and translucency of the zebrafish, this model may afford a high throughput system for characterization of novel PGES inhibitors. |
doi_str_mv | 10.1161/01.ATV.0000152355.97808.10 |
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METHODS AND RESULTS—We cloned zebrafish cytosolic (c) and m-PGES orthologs and mapped them to syntenic regions of chromosomes 23 and 5. cPGES was widely expressed during development and was coordinately regulated with zCOX-1 in the inner ear, the pronephros, and intestine. COX-2 and mPGES-1 exhibited restricted expression, dominantly in the vasculature of the aortic arch. However, the enzymes were anatomically segregated within the vessel wall. Experiments with antisense morpholinos and with nonsteroidal antiinflammatory drugs suggest that these genes may not be critical for development.
CONCLUSIONS—mPGES-1 is developmentally coregulated with COX-2 in vasculature. Given the high fecundidity and translucency of the zebrafish, this model may afford a high throughput system for characterization of novel PGES inhibitors.</description><identifier>ISSN: 1079-5642</identifier><identifier>EISSN: 1524-4636</identifier><identifier>DOI: 10.1161/01.ATV.0000152355.97808.10</identifier><identifier>PMID: 15576635</identifier><identifier>CODEN: ATVBFA</identifier><language>eng</language><publisher>Philadelphia, PA: American Heart Association, Inc</publisher><subject>Animals ; Atherosclerosis (general aspects, experimental research) ; Biological and medical sciences ; Blood and lymphatic vessels ; Blood Vessels - embryology ; Blood Vessels - enzymology ; Blood Vessels - growth & development ; Cardiology. Vascular system ; Chromosome Mapping ; Cyclooxygenase 1 ; Cyclooxygenase 2 ; Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous ; Embryo, Nonmammalian ; Gene Expression Regulation, Developmental ; Gene Expression Regulation, Enzymologic ; Intramolecular Oxidoreductases - biosynthesis ; Intramolecular Oxidoreductases - genetics ; Isoenzymes - biosynthesis ; Isoenzymes - genetics ; Kidney - embryology ; Kidney - enzymology ; Kidney - growth & development ; Larva ; Medical sciences ; Microsomes - enzymology ; Molecular Sequence Data ; Organ Specificity ; Phylogeny ; Prostaglandin-E Synthases ; Prostaglandin-Endoperoxide Synthases - biosynthesis ; Prostaglandin-Endoperoxide Synthases - genetics ; Prostaglandins E - biosynthesis ; Semicircular Canals - embryology ; Semicircular Canals - enzymology ; Semicircular Canals - growth & development ; Zebrafish - growth & development ; Zebrafish - metabolism ; Zebrafish Proteins - biosynthesis ; Zebrafish Proteins - genetics</subject><ispartof>Arteriosclerosis, thrombosis, and vascular biology, 2005-02, Vol.25 (2), p.315-320</ispartof><rights>2005 American Heart Association, Inc.</rights><rights>2005 INIST-CNRS</rights><rights>Copyright American Heart Association, Inc. Feb 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5703-5bee682583610c1868d0b428c46a82d2d7ca71544e73885853fac64abbeda2c63</citedby><cites>FETCH-LOGICAL-c5703-5bee682583610c1868d0b428c46a82d2d7ca71544e73885853fac64abbeda2c63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16514774$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15576635$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Pini, Barbara</creatorcontrib><creatorcontrib>Grosser, Tilo</creatorcontrib><creatorcontrib>Lawson, John A</creatorcontrib><creatorcontrib>Price, Tom S</creatorcontrib><creatorcontrib>Pack, Michael A</creatorcontrib><creatorcontrib>FitzGerald, Garret A</creatorcontrib><title>Prostaglandin E Synthases in Zebrafish</title><title>Arteriosclerosis, thrombosis, and vascular biology</title><addtitle>Arterioscler Thromb Vasc Biol</addtitle><description>OBJECTIVE—Prostaglandin E synthases (PGESs) are being explored as antiinflammtory drug targets as alternatives to cyclooxygenase (COX)-2. Located downstream of the cyclooxygenases, PGESs catalyze PGE2 formation, and deletion of microsomal (m)-PGES-1 abrogates inflammation. We sought to characterize the developmental expression of COX and PGES in zebrafish.
METHODS AND RESULTS—We cloned zebrafish cytosolic (c) and m-PGES orthologs and mapped them to syntenic regions of chromosomes 23 and 5. cPGES was widely expressed during development and was coordinately regulated with zCOX-1 in the inner ear, the pronephros, and intestine. COX-2 and mPGES-1 exhibited restricted expression, dominantly in the vasculature of the aortic arch. However, the enzymes were anatomically segregated within the vessel wall. Experiments with antisense morpholinos and with nonsteroidal antiinflammatory drugs suggest that these genes may not be critical for development.
CONCLUSIONS—mPGES-1 is developmentally coregulated with COX-2 in vasculature. Given the high fecundidity and translucency of the zebrafish, this model may afford a high throughput system for characterization of novel PGES inhibitors.</description><subject>Animals</subject><subject>Atherosclerosis (general aspects, experimental research)</subject><subject>Biological and medical sciences</subject><subject>Blood and lymphatic vessels</subject><subject>Blood Vessels - embryology</subject><subject>Blood Vessels - enzymology</subject><subject>Blood Vessels - growth & development</subject><subject>Cardiology. Vascular system</subject><subject>Chromosome Mapping</subject><subject>Cyclooxygenase 1</subject><subject>Cyclooxygenase 2</subject><subject>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</subject><subject>Embryo, Nonmammalian</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>Intramolecular Oxidoreductases - biosynthesis</subject><subject>Intramolecular Oxidoreductases - genetics</subject><subject>Isoenzymes - biosynthesis</subject><subject>Isoenzymes - genetics</subject><subject>Kidney - embryology</subject><subject>Kidney - enzymology</subject><subject>Kidney - growth & development</subject><subject>Larva</subject><subject>Medical sciences</subject><subject>Microsomes - enzymology</subject><subject>Molecular Sequence Data</subject><subject>Organ Specificity</subject><subject>Phylogeny</subject><subject>Prostaglandin-E Synthases</subject><subject>Prostaglandin-Endoperoxide Synthases - biosynthesis</subject><subject>Prostaglandin-Endoperoxide Synthases - genetics</subject><subject>Prostaglandins E - biosynthesis</subject><subject>Semicircular Canals - embryology</subject><subject>Semicircular Canals - enzymology</subject><subject>Semicircular Canals - growth & development</subject><subject>Zebrafish - growth & development</subject><subject>Zebrafish - metabolism</subject><subject>Zebrafish Proteins - biosynthesis</subject><subject>Zebrafish Proteins - genetics</subject><issn>1079-5642</issn><issn>1524-4636</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpdkFtLxDAQhYMo3v-CLIK-tU4uk2R9k8UbCAquPvgS0jR1q91Wk5bFf2_WXVgwD7nxzZwzh5BTCjmlkl4Aza-mrzmkRZFxxHysNOicwhbZTz8iE5LL7XQHNc5QCrZHDmL8SLxgDHbJHkVUUnLcJ-dPoYu9fW9sW9bt6Hr0_NP2Mxt9HKXnmy-Creo4OyI7lW2iP16fh-Tl5no6ucseHm_vJ1cPmUMFPMPCe6kZai4pOKqlLqEQTDshrWYlK5WziqIQXnGtUSOvrJPCFoUvLXOSH5LzVd-v0H0PPvZmXkfnm2TPd0M0MtXJMYUEnv4DP7ohtMmbYWlKPUbOE3S5glwaMgZfma9Qz234MRTMMkoD1KQozSZK8xel-VM4WSsMxdyXm9J1dgk4WwM2OttUwbaujhtOIhVKicSJFbfomt6H-NkMCx_MzNumny2lBZeAGQNASBtkSzOc_wIDx4ks</recordid><startdate>200502</startdate><enddate>200502</enddate><creator>Pini, Barbara</creator><creator>Grosser, Tilo</creator><creator>Lawson, John A</creator><creator>Price, Tom S</creator><creator>Pack, Michael A</creator><creator>FitzGerald, Garret A</creator><general>American Heart Association, Inc</general><general>Lippincott</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>K9.</scope><scope>7X8</scope></search><sort><creationdate>200502</creationdate><title>Prostaglandin E Synthases in Zebrafish</title><author>Pini, Barbara ; Grosser, Tilo ; Lawson, John A ; Price, Tom S ; Pack, Michael A ; FitzGerald, Garret A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5703-5bee682583610c1868d0b428c46a82d2d7ca71544e73885853fac64abbeda2c63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Atherosclerosis (general aspects, experimental research)</topic><topic>Biological and medical sciences</topic><topic>Blood and lymphatic vessels</topic><topic>Blood Vessels - embryology</topic><topic>Blood Vessels - enzymology</topic><topic>Blood Vessels - growth & development</topic><topic>Cardiology. Vascular system</topic><topic>Chromosome Mapping</topic><topic>Cyclooxygenase 1</topic><topic>Cyclooxygenase 2</topic><topic>Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous</topic><topic>Embryo, Nonmammalian</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>Intramolecular Oxidoreductases - biosynthesis</topic><topic>Intramolecular Oxidoreductases - genetics</topic><topic>Isoenzymes - biosynthesis</topic><topic>Isoenzymes - genetics</topic><topic>Kidney - embryology</topic><topic>Kidney - enzymology</topic><topic>Kidney - growth & development</topic><topic>Larva</topic><topic>Medical sciences</topic><topic>Microsomes - enzymology</topic><topic>Molecular Sequence Data</topic><topic>Organ Specificity</topic><topic>Phylogeny</topic><topic>Prostaglandin-E Synthases</topic><topic>Prostaglandin-Endoperoxide Synthases - biosynthesis</topic><topic>Prostaglandin-Endoperoxide Synthases - genetics</topic><topic>Prostaglandins E - biosynthesis</topic><topic>Semicircular Canals - embryology</topic><topic>Semicircular Canals - enzymology</topic><topic>Semicircular Canals - growth & development</topic><topic>Zebrafish - growth & development</topic><topic>Zebrafish - metabolism</topic><topic>Zebrafish Proteins - biosynthesis</topic><topic>Zebrafish Proteins - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Pini, Barbara</creatorcontrib><creatorcontrib>Grosser, Tilo</creatorcontrib><creatorcontrib>Lawson, John A</creatorcontrib><creatorcontrib>Price, Tom S</creatorcontrib><creatorcontrib>Pack, Michael A</creatorcontrib><creatorcontrib>FitzGerald, Garret A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>MEDLINE - Academic</collection><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Pini, Barbara</au><au>Grosser, Tilo</au><au>Lawson, John A</au><au>Price, Tom S</au><au>Pack, Michael A</au><au>FitzGerald, Garret A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Prostaglandin E Synthases in Zebrafish</atitle><jtitle>Arteriosclerosis, thrombosis, and vascular biology</jtitle><addtitle>Arterioscler Thromb Vasc Biol</addtitle><date>2005-02</date><risdate>2005</risdate><volume>25</volume><issue>2</issue><spage>315</spage><epage>320</epage><pages>315-320</pages><issn>1079-5642</issn><eissn>1524-4636</eissn><coden>ATVBFA</coden><abstract>OBJECTIVE—Prostaglandin E synthases (PGESs) are being explored as antiinflammtory drug targets as alternatives to cyclooxygenase (COX)-2. Located downstream of the cyclooxygenases, PGESs catalyze PGE2 formation, and deletion of microsomal (m)-PGES-1 abrogates inflammation. We sought to characterize the developmental expression of COX and PGES in zebrafish.
METHODS AND RESULTS—We cloned zebrafish cytosolic (c) and m-PGES orthologs and mapped them to syntenic regions of chromosomes 23 and 5. cPGES was widely expressed during development and was coordinately regulated with zCOX-1 in the inner ear, the pronephros, and intestine. COX-2 and mPGES-1 exhibited restricted expression, dominantly in the vasculature of the aortic arch. However, the enzymes were anatomically segregated within the vessel wall. Experiments with antisense morpholinos and with nonsteroidal antiinflammatory drugs suggest that these genes may not be critical for development.
CONCLUSIONS—mPGES-1 is developmentally coregulated with COX-2 in vasculature. Given the high fecundidity and translucency of the zebrafish, this model may afford a high throughput system for characterization of novel PGES inhibitors.</abstract><cop>Philadelphia, PA</cop><cop>Hagerstown, MD</cop><pub>American Heart Association, Inc</pub><pmid>15576635</pmid><doi>10.1161/01.ATV.0000152355.97808.10</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Atherosclerosis (general aspects, experimental research) Biological and medical sciences Blood and lymphatic vessels Blood Vessels - embryology Blood Vessels - enzymology Blood Vessels - growth & development Cardiology. Vascular system Chromosome Mapping Cyclooxygenase 1 Cyclooxygenase 2 Diseases of the peripheral vessels. Diseases of the vena cava. Miscellaneous Embryo, Nonmammalian Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic Intramolecular Oxidoreductases - biosynthesis Intramolecular Oxidoreductases - genetics Isoenzymes - biosynthesis Isoenzymes - genetics Kidney - embryology Kidney - enzymology Kidney - growth & development Larva Medical sciences Microsomes - enzymology Molecular Sequence Data Organ Specificity Phylogeny Prostaglandin-E Synthases Prostaglandin-Endoperoxide Synthases - biosynthesis Prostaglandin-Endoperoxide Synthases - genetics Prostaglandins E - biosynthesis Semicircular Canals - embryology Semicircular Canals - enzymology Semicircular Canals - growth & development Zebrafish - growth & development Zebrafish - metabolism Zebrafish Proteins - biosynthesis Zebrafish Proteins - genetics |
title | Prostaglandin E Synthases in Zebrafish |
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