Separation of 2-Aminobenzoic Acid-Derivatized Glycosaminoglycans and Asparagine-linked Glycans by Capillary Electrophoresis
A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan Δ-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. Th...
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| Veröffentlicht in: | Analytical Sciences 2005, Vol.21(1), pp.21-24 |
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| creator | SATO, Kae SATO, Kiichi OKUBO, Akira YAMAZAKI, Sunao |
| description | A capillary electrophoresis method was developed for the analysis of oligosaccharides combined with derivatization with 2-aminobenzoic acid. Glycosaminoglycan Δ-disaccharides were effectively resolved on a fused-silica capillary tube using 150 mM borate, pH 8.5, as a running electrolyte solution. This analytical method was applied to the identification of glycosaminoglycan in combination with enzymatic digestion. The separation of N-glycans or glucose-oligomers was performed with a phosphate buffer containing polyethylene glycol or borate as an electrolyte solution. This method is expected to be useful in the determination of oligosaccharide structures in a glycoprotein. |
| doi_str_mv | 10.2116/analsci.21.21 |
| format | Article |
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| language | eng |
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| subjects | Animals Asparagine Chondroitin ABC Lyase - metabolism Disaccharides - analysis Disaccharides - isolation & purification Electrophoresis, Capillary - methods Glycosaminoglycans - isolation & purification Glycosaminoglycans - metabolism Oligosaccharides - analysis Oligosaccharides - isolation & purification ortho-Aminobenzoates Peptide Hydrolases - metabolism Polysaccharides - isolation & purification Polysaccharides - metabolism |
| title | Separation of 2-Aminobenzoic Acid-Derivatized Glycosaminoglycans and Asparagine-linked Glycans by Capillary Electrophoresis |
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