Relative expression of Pseudomonas aeruginosa virulence genes analyzed by a real time RT-PCR method during lung infection in rats
The bacterial aspects of the events occurring during the first days of lung colonization by Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three P. aeruginosa virulence genes: exoS, lasI and...
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| Veröffentlicht in: | FEMS microbiology letters 2005-02, Vol.243 (1), p.271-278 |
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| creator | Béatrice, Joly Maud, Pierre Stéphane, Auvin Franc¸ cois, Collet Frédéric, Gottrand Benoit, Guery Marie-Odile, Husson |
| description | The bacterial aspects of the events occurring during the first days of lung colonization by
Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three
P. aeruginosa virulence genes:
exoS,
lasI and
algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout
P. aeruginosa infection.
ExoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of
algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of
exoS and
algD. The strong expression of
exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of
algD expression after this period was associated to the development of a chronic infection with no further mortality.
LasI transcription remained more constant throughout the infection. |
| doi_str_mv | 10.1016/j.femsle.2004.12.012 |
| format | Article |
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Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three
P. aeruginosa virulence genes:
exoS,
lasI and
algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout
P. aeruginosa infection.
ExoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of
algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of
exoS and
algD. The strong expression of
exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of
algD expression after this period was associated to the development of a chronic infection with no further mortality.
LasI transcription remained more constant throughout the infection.</description><identifier>ISSN: 0378-1097</identifier><identifier>EISSN: 1574-6968</identifier><identifier>DOI: 10.1016/j.femsle.2004.12.012</identifier><identifier>PMID: 15668029</identifier><identifier>CODEN: FMLED7</identifier><language>eng</language><publisher>Oxford, UK: Elsevier B.V</publisher><subject>ADP Ribose Transferases - genetics ; ADP Ribose Transferases - metabolism ; Animals ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacterial Toxins - genetics ; Bacterial Toxins - metabolism ; Bacteriological methods and techniques used in bacteriology ; Bacteriology ; Biological and medical sciences ; Bronchopulmonary infection ; Chronic infection ; Colonization ; Experimental lung infection ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial ; Genes ; Humans ; Infections ; Lung Diseases - microbiology ; Lungs ; Microbiology ; Mortality ; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains ; Polymerase chain reaction ; Pseudomonas aeruginosa ; Pseudomonas aeruginosa - genetics ; Pseudomonas aeruginosa - metabolism ; Pseudomonas aeruginosa - pathogenicity ; Pseudomonas Infections - microbiology ; Rats ; Rats, Sprague-Dawley ; Real time ; Real time RT-PCR ; Reverse Transcriptase Polymerase Chain Reaction - methods ; Transcription ; Transcription, Genetic ; Virulence ; Virulence Factors - genetics ; Virulence Factors - metabolism ; Virulence genes</subject><ispartof>FEMS microbiology letters, 2005-02, Vol.243 (1), p.271-278</ispartof><rights>2004 Federation of European Microbiological Societies</rights><rights>2004 Federation of European Microbiological Societies 2004</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5381-7d587461d04e9a155b191c032cc4358d431c1ba285e10534fbaf84b4b8363a173</citedby><cites>FETCH-LOGICAL-c5381-7d587461d04e9a155b191c032cc4358d431c1ba285e10534fbaf84b4b8363a173</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1016%2Fj.femsle.2004.12.012$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1016%2Fj.femsle.2004.12.012$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16461235$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15668029$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Béatrice, Joly</creatorcontrib><creatorcontrib>Maud, Pierre</creatorcontrib><creatorcontrib>Stéphane, Auvin</creatorcontrib><creatorcontrib>Franc¸ cois, Collet</creatorcontrib><creatorcontrib>Frédéric, Gottrand</creatorcontrib><creatorcontrib>Benoit, Guery</creatorcontrib><creatorcontrib>Marie-Odile, Husson</creatorcontrib><title>Relative expression of Pseudomonas aeruginosa virulence genes analyzed by a real time RT-PCR method during lung infection in rats</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>The bacterial aspects of the events occurring during the first days of lung colonization by
Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three
P. aeruginosa virulence genes:
exoS,
lasI and
algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout
P. aeruginosa infection.
ExoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of
algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of
exoS and
algD. The strong expression of
exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of
algD expression after this period was associated to the development of a chronic infection with no further mortality.
LasI transcription remained more constant throughout the infection.</description><subject>ADP Ribose Transferases - genetics</subject><subject>ADP Ribose Transferases - metabolism</subject><subject>Animals</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacterial Toxins - genetics</subject><subject>Bacterial Toxins - metabolism</subject><subject>Bacteriological methods and techniques used in bacteriology</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Bronchopulmonary infection</subject><subject>Chronic infection</subject><subject>Colonization</subject><subject>Experimental lung infection</subject><subject>Fundamental and applied biological sciences. 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Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Béatrice, Joly</au><au>Maud, Pierre</au><au>Stéphane, Auvin</au><au>Franc¸ cois, Collet</au><au>Frédéric, Gottrand</au><au>Benoit, Guery</au><au>Marie-Odile, Husson</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Relative expression of Pseudomonas aeruginosa virulence genes analyzed by a real time RT-PCR method during lung infection in rats</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>2005-02</date><risdate>2005</risdate><volume>243</volume><issue>1</issue><spage>271</spage><epage>278</epage><pages>271-278</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>The bacterial aspects of the events occurring during the first days of lung colonization by
Pseudomonas aeruginosa are still unknown. The aim of this study was to develop a real time RT-PCR method for the direct quantification of the transcripts of three
P. aeruginosa virulence genes:
exoS,
lasI and
algD, during the first seven days of a rat lung infection. Our results document differences in bacterial gene expression throughout
P. aeruginosa infection.
ExoS transcripts levels were very high in the first days of infection, but a significant decrease was then progressively observed. Transcription of
algD occurred on the 4th day and increased regularly over time suggesting a balance in the transcription of
exoS and
algD. The strong expression of
exoS during the first 3 days was correlated to 29% of mortality among infected rats. On the contrary, the increase of
algD expression after this period was associated to the development of a chronic infection with no further mortality.
LasI transcription remained more constant throughout the infection.</abstract><cop>Oxford, UK</cop><pub>Elsevier B.V</pub><pmid>15668029</pmid><doi>10.1016/j.femsle.2004.12.012</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 2005-02, Vol.243 (1), p.271-278 |
| issn | 0378-1097 1574-6968 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_67378585 |
| source | MEDLINE; Wiley Online Library Journals Frontfile Complete; Oxford University Press Journals All Titles (1996-Current); Alma/SFX Local Collection |
| subjects | ADP Ribose Transferases - genetics ADP Ribose Transferases - metabolism Animals Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacterial Toxins - genetics Bacterial Toxins - metabolism Bacteriological methods and techniques used in bacteriology Bacteriology Biological and medical sciences Bronchopulmonary infection Chronic infection Colonization Experimental lung infection Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genes Humans Infections Lung Diseases - microbiology Lungs Microbiology Mortality Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains Polymerase chain reaction Pseudomonas aeruginosa Pseudomonas aeruginosa - genetics Pseudomonas aeruginosa - metabolism Pseudomonas aeruginosa - pathogenicity Pseudomonas Infections - microbiology Rats Rats, Sprague-Dawley Real time Real time RT-PCR Reverse Transcriptase Polymerase Chain Reaction - methods Transcription Transcription, Genetic Virulence Virulence Factors - genetics Virulence Factors - metabolism Virulence genes |
| title | Relative expression of Pseudomonas aeruginosa virulence genes analyzed by a real time RT-PCR method during lung infection in rats |
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