Molecular cloning and transcriptional activation of lysozyme-encoding cDNAs in the mosquito Aedes aegypti
Lysozymes are enzymes characterized by their ability to break down bacterial cell walls. In insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. We identified two lysozyme-encoding cDNAs from Aedes aegypti. Both...
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| Veröffentlicht in: | Insect molecular biology 2005, Vol.14 (1), p.89-94 |
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| creator | Ursic Bedoya, R.J Mitzey, A.M Obraztsova, M Lowenberger, C |
| description | Lysozymes are enzymes characterized by their ability to break down bacterial cell walls. In insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. We identified two lysozyme-encoding cDNAs from Aedes aegypti. Both deduced protein sequences are basic in nature, contain 148 amino acids including eight highly conserved cysteine residues, and their genomic sequences contain a single intron. Transcriptional profiles indicated that the predominant form is constitutively expressed and up-regulated upon immune challenge and blood feeding in adult mosquitoes. The second form is expressed during early developmental stages, larvae and pupae, and at low levels in adults after immune challenge. Lysozymes in Aedes aegypti play both roles, defined by the spatial and temporal regulation of their expression. |
| doi_str_mv | 10.1111/j.1365-2583.2004.00534.x |
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In insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. We identified two lysozyme-encoding cDNAs from Aedes aegypti. Both deduced protein sequences are basic in nature, contain 148 amino acids including eight highly conserved cysteine residues, and their genomic sequences contain a single intron. Transcriptional profiles indicated that the predominant form is constitutively expressed and up-regulated upon immune challenge and blood feeding in adult mosquitoes. The second form is expressed during early developmental stages, larvae and pupae, and at low levels in adults after immune challenge. 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In insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. We identified two lysozyme-encoding cDNAs from Aedes aegypti. Both deduced protein sequences are basic in nature, contain 148 amino acids including eight highly conserved cysteine residues, and their genomic sequences contain a single intron. Transcriptional profiles indicated that the predominant form is constitutively expressed and up-regulated upon immune challenge and blood feeding in adult mosquitoes. The second form is expressed during early developmental stages, larvae and pupae, and at low levels in adults after immune challenge. Lysozymes in Aedes aegypti play both roles, defined by the spatial and temporal regulation of their expression.</description><subject>Aedes - enzymology</subject><subject>Aedes - genetics</subject><subject>Aedes - growth & development</subject><subject>Aedes aegypti</subject><subject>Amino Acid Sequence</subject><subject>amino acid sequences</subject><subject>Animals</subject><subject>bacterial infections</subject><subject>Base Sequence</subject><subject>Cloning, Molecular</subject><subject>complementary DNA</subject><subject>developmental stages</subject><subject>Freshwater</subject><subject>gene expression regulation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Gene Expression Regulation, Enzymologic</subject><subject>gene induction</subject><subject>hematophagy</subject><subject>insect immunity</subject><subject>lysozyme</subject><subject>Molecular Sequence Data</subject><subject>Muramidase - biosynthesis</subject><subject>Muramidase - genetics</subject><subject>nucleotide sequences</subject><subject>Phylogeny</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>RNA, Messenger - biosynthesis</subject><subject>RNA, Messenger - genetics</subject><subject>Sequence Alignment</subject><subject>Transcriptional Activation</subject><issn>0962-1075</issn><issn>1365-2583</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkU-P1CAchonRuOPqV1BO3lp_QEtp4mXc_3F3zaobvRGGwsjYlllo3amf3nY7WY_KBQjP8xJ4EcIEUjKOd5uUMJ4nNBcspQBZCpCzLN09QYvHg6doASWnCYEiP0AvYtwAgCh5-RwdkJxzVhRigdyVr43uaxWwrn3r2jVWbYW7oNqog9t2zreqxkp37peaNthbXA_R_x4ak5hW-2py9PH1MmLX4u6HwY2Pd73rPF6aykSszHoYc16iZ1bV0bzaz4fo9vTk69F5cvnp7OJoeZnoLC-zpOTWclB2RQQpbFUyI0CoShDIWGXKTGlmC8WMYUwRWxJS8BVQSy1bMSIUsEP0ds7dBn_Xm9jJxkVt6lq1xvdR8oIVIqP_BsdkQnNKRlDMoA4-xmCs3AbXqDBIAnLqQ27k9O1y-nY59SEf-pC7UX29v6NfNab6K-4LGIH3M3DvajP8d7C8uPowLkY9mXUXO7N71FX4-fDOXH67PpOfPxY35Pjmuzwd-Tczb5WXah1clLdfKBAGBIBwTtkf72uyhQ</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Ursic Bedoya, R.J</creator><creator>Mitzey, A.M</creator><creator>Obraztsova, M</creator><creator>Lowenberger, C</creator><general>Blackwell Science Ltd</general><scope>FBQ</scope><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7SS</scope><scope>8FD</scope><scope>F1W</scope><scope>FR3</scope><scope>H95</scope><scope>L.G</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Molecular cloning and transcriptional activation of lysozyme-encoding cDNAs in the mosquito Aedes aegypti</title><author>Ursic Bedoya, R.J ; Mitzey, A.M ; Obraztsova, M ; Lowenberger, C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4594-96ff60afb1817fd93e808ad81043de94ac3f7a3ee33a1f91176b02f2f3b318a03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Aedes - enzymology</topic><topic>Aedes - genetics</topic><topic>Aedes - growth & development</topic><topic>Aedes aegypti</topic><topic>Amino Acid Sequence</topic><topic>amino acid sequences</topic><topic>Animals</topic><topic>bacterial infections</topic><topic>Base Sequence</topic><topic>Cloning, Molecular</topic><topic>complementary DNA</topic><topic>developmental stages</topic><topic>Freshwater</topic><topic>gene expression regulation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Gene Expression Regulation, Enzymologic</topic><topic>gene induction</topic><topic>hematophagy</topic><topic>insect immunity</topic><topic>lysozyme</topic><topic>Molecular Sequence Data</topic><topic>Muramidase - biosynthesis</topic><topic>Muramidase - genetics</topic><topic>nucleotide sequences</topic><topic>Phylogeny</topic><topic>Reverse Transcriptase Polymerase Chain Reaction</topic><topic>RNA, Messenger - biosynthesis</topic><topic>RNA, Messenger - genetics</topic><topic>Sequence Alignment</topic><topic>Transcriptional Activation</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ursic Bedoya, R.J</creatorcontrib><creatorcontrib>Mitzey, A.M</creatorcontrib><creatorcontrib>Obraztsova, M</creatorcontrib><creatorcontrib>Lowenberger, C</creatorcontrib><collection>AGRIS</collection><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Technology Research Database</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Insect molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ursic Bedoya, R.J</au><au>Mitzey, A.M</au><au>Obraztsova, M</au><au>Lowenberger, C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and transcriptional activation of lysozyme-encoding cDNAs in the mosquito Aedes aegypti</atitle><jtitle>Insect molecular biology</jtitle><addtitle>Insect Mol Biol</addtitle><date>2005</date><risdate>2005</risdate><volume>14</volume><issue>1</issue><spage>89</spage><epage>94</epage><pages>89-94</pages><issn>0962-1075</issn><eissn>1365-2583</eissn><abstract>Lysozymes are enzymes characterized by their ability to break down bacterial cell walls. In insects certain lysozymes are only found in the midgut, whereas others are only found in the haemolymph and fat body after immune challenge. We identified two lysozyme-encoding cDNAs from Aedes aegypti. Both deduced protein sequences are basic in nature, contain 148 amino acids including eight highly conserved cysteine residues, and their genomic sequences contain a single intron. Transcriptional profiles indicated that the predominant form is constitutively expressed and up-regulated upon immune challenge and blood feeding in adult mosquitoes. The second form is expressed during early developmental stages, larvae and pupae, and at low levels in adults after immune challenge. Lysozymes in Aedes aegypti play both roles, defined by the spatial and temporal regulation of their expression.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15663778</pmid><doi>10.1111/j.1365-2583.2004.00534.x</doi><tpages>6</tpages></addata></record> |
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| subjects | Aedes - enzymology Aedes - genetics Aedes - growth & development Aedes aegypti Amino Acid Sequence amino acid sequences Animals bacterial infections Base Sequence Cloning, Molecular complementary DNA developmental stages Freshwater gene expression regulation Gene Expression Regulation, Developmental Gene Expression Regulation, Enzymologic gene induction hematophagy insect immunity lysozyme Molecular Sequence Data Muramidase - biosynthesis Muramidase - genetics nucleotide sequences Phylogeny Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger - biosynthesis RNA, Messenger - genetics Sequence Alignment Transcriptional Activation |
| title | Molecular cloning and transcriptional activation of lysozyme-encoding cDNAs in the mosquito Aedes aegypti |
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