The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes

We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanoso...

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Veröffentlicht in:Molecular and biochemical parasitology 2005-02, Vol.139 (2), p.163-172
Hauptverfasser: Brems, Stefanie, Lys Guilbride, D., Gundlesdodjir-Planck, Drifa, Busold, Christian, Luu, Van-Duc, Schanne, Michaela, Hoheisel, Jörg, Clayton, Christine
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container_end_page 172
container_issue 2
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container_title Molecular and biochemical parasitology
container_volume 139
creator Brems, Stefanie
Lys Guilbride, D.
Gundlesdodjir-Planck, Drifa
Busold, Christian
Luu, Van-Duc
Schanne, Michaela
Hoheisel, Jörg
Clayton, Christine
description We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes – encoding HSP83 and an importin-related protein – appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level.
doi_str_mv 10.1016/j.molbiopara.2004.11.004
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subjects Animals
Array
Blood - parasitology
Differentiation
Gene Expression Regulation, Developmental
Oligonucleotide Array Sequence Analysis
Proteome
Protozoan Proteins - genetics
Protozoan Proteins - metabolism
Regulation
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcription, Genetic
Trypanosoma
Trypanosoma brucei
Trypanosoma brucei brucei - genetics
Trypanosoma brucei brucei - growth & development
Trypanosoma brucei brucei - metabolism
title The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes
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