The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes
We describe developmentally regulated genes in two strains of Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanoso...
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Veröffentlicht in: | Molecular and biochemical parasitology 2005-02, Vol.139 (2), p.163-172 |
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container_title | Molecular and biochemical parasitology |
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creator | Brems, Stefanie Lys Guilbride, D. Gundlesdodjir-Planck, Drifa Busold, Christian Luu, Van-Duc Schanne, Michaela Hoheisel, Jörg Clayton, Christine |
description | We describe developmentally regulated genes in two strains of
Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes – encoding HSP83 and an importin-related protein – appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level. |
doi_str_mv | 10.1016/j.molbiopara.2004.11.004 |
format | Article |
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Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes – encoding HSP83 and an importin-related protein – appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level.</description><subject>Animals</subject><subject>Array</subject><subject>Blood - parasitology</subject><subject>Differentiation</subject><subject>Gene Expression Regulation, Developmental</subject><subject>Oligonucleotide Array Sequence Analysis</subject><subject>Proteome</subject><subject>Protozoan Proteins - genetics</subject><subject>Protozoan Proteins - metabolism</subject><subject>Regulation</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Transcription, Genetic</subject><subject>Trypanosoma</subject><subject>Trypanosoma brucei</subject><subject>Trypanosoma brucei brucei - genetics</subject><subject>Trypanosoma brucei brucei - growth & development</subject><subject>Trypanosoma brucei brucei - metabolism</subject><issn>0166-6851</issn><issn>1872-9428</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU9v1DAQxS0EapfSr4B84pbgif_FR6hKi7QSEtqeLceZBa-SONhepP32uOxKPfb0RuPfm7HmEUKBtcBAfT60c5yGEFeXXNsxJlqAtsobsoFed40RXf-WbCqqGtVLuCbvcz4wxqRW6opcg1RKKAkbEna_kZbkluxTWEucMdO4p7t0Wt0Sc5wdHdLRY6DbkAsmKjpN3TLS3c_7J1PrYYpxzCWhm__31xT9yU_B16mntfpzCb9iwfyBvNu7KePtRW_I07f73d1js_3x8P3uy7bxQvLSaAHaiEFq7DrVSTPsUckBeugQR2a84Mpw6blmxngPtYmIko1G96w-GX5DPp3n1p_8OWIudg7Z4zS5BeMxW6W55iD4qyAYyRUTqoL9GfQp5pxwb9cUZpdOFph9zsMe7Ese9jkPC2CrVOvHy47jMOP4YrwEUIGvZwDrSf4GTDb7gIvHMST0xY4xvL7lH8KHoV4</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Brems, Stefanie</creator><creator>Lys Guilbride, D.</creator><creator>Gundlesdodjir-Planck, Drifa</creator><creator>Busold, Christian</creator><creator>Luu, Van-Duc</creator><creator>Schanne, Michaela</creator><creator>Hoheisel, Jörg</creator><creator>Clayton, Christine</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7TM</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes</title><author>Brems, Stefanie ; Lys Guilbride, D. ; Gundlesdodjir-Planck, Drifa ; Busold, Christian ; Luu, Van-Duc ; Schanne, Michaela ; Hoheisel, Jörg ; Clayton, Christine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c453t-741794b57e226259bfe65b1812eed09c436935c37099cc1eedeee50d978036993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Array</topic><topic>Blood - parasitology</topic><topic>Differentiation</topic><topic>Gene Expression Regulation, Developmental</topic><topic>Oligonucleotide Array Sequence Analysis</topic><topic>Proteome</topic><topic>Protozoan Proteins - genetics</topic><topic>Protozoan Proteins - metabolism</topic><topic>Regulation</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Transcription, Genetic</topic><topic>Trypanosoma</topic><topic>Trypanosoma brucei</topic><topic>Trypanosoma brucei brucei - genetics</topic><topic>Trypanosoma brucei brucei - growth & development</topic><topic>Trypanosoma brucei brucei - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brems, Stefanie</creatorcontrib><creatorcontrib>Lys Guilbride, D.</creatorcontrib><creatorcontrib>Gundlesdodjir-Planck, Drifa</creatorcontrib><creatorcontrib>Busold, Christian</creatorcontrib><creatorcontrib>Luu, Van-Duc</creatorcontrib><creatorcontrib>Schanne, Michaela</creatorcontrib><creatorcontrib>Hoheisel, Jörg</creatorcontrib><creatorcontrib>Clayton, Christine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular and biochemical parasitology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brems, Stefanie</au><au>Lys Guilbride, D.</au><au>Gundlesdodjir-Planck, Drifa</au><au>Busold, Christian</au><au>Luu, Van-Duc</au><au>Schanne, Michaela</au><au>Hoheisel, Jörg</au><au>Clayton, Christine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes</atitle><jtitle>Molecular and biochemical parasitology</jtitle><addtitle>Mol Biochem Parasitol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>139</volume><issue>2</issue><spage>163</spage><epage>172</epage><pages>163-172</pages><issn>0166-6851</issn><eissn>1872-9428</eissn><abstract>We describe developmentally regulated genes in two strains of
Trypanosoma brucei: the monomorphic strain Lister 427 and the pleomorphic strain TREU927. Expression patterns were obtained using an array of 24,567 genomic fragments. Probes were prepared from bloodstream-form or procyclic-form trypanosomes. Fourteen procyclic-specific and 77 bloodstream-specific signals were obtained from sequences matching variant surface glycoprotein or associated genes, and a further 17 regulated sequences were repetitive or transposable-element-related. Two hundred and eighty-six regulated spots corresponded to mRNAs from other protein-coding genes; these spots represent 191 different proteins. Regulation of 113 different genes (79 from procyclic forms, 34 from bloodstream-forms) was supported by at least two independent experiments or criteria; of these, about 60 were novel. Only two genes – encoding HSP83 and an importin-related protein – appeared to be regulated in the TREU927 strain only. Our results confirmed previous estimates that 2% of trypanosome genes show developmental regulation at the mRNA level.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>15664651</pmid><doi>10.1016/j.molbiopara.2004.11.004</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Animals Array Blood - parasitology Differentiation Gene Expression Regulation, Developmental Oligonucleotide Array Sequence Analysis Proteome Protozoan Proteins - genetics Protozoan Proteins - metabolism Regulation RNA, Messenger - genetics RNA, Messenger - metabolism Transcription, Genetic Trypanosoma Trypanosoma brucei Trypanosoma brucei brucei - genetics Trypanosoma brucei brucei - growth & development Trypanosoma brucei brucei - metabolism |
title | The transcriptomes of Trypanosoma brucei Lister 427 and TREU927 bloodstream and procyclic trypomastigotes |
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