Competitive homogeneous digoxigenin immunoassay based on fluorescence quenching by gold nanoparticles

We report on a competitive, homogeneous immunoassay for the detection of the hapten digoxigenin. The assay is based on competitive fluorescence quenching by gold nanoparticles. Digoxigenin is indirectly labeled with the fluorophore Cy3B through bovine serum albumin and used as a marker. Gold nanopar...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Analytica chimica acta 2009-07, Vol.646 (1), p.119-122
Hauptverfasser:	Mayilo, Sergiy, Ehlers, Benjamin, Wunderlich, Michael, Klar, Thomas A., Josel, Hans-Peter, Heindl, Dieter, Nichtl, Alfons, Kürzinger, Konrad, Feldmann, Jochen
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Animals Antibodies - chemistry Antibodies - immunology Cattle Chemistry Digoxigenin Digoxigenin - analysis Digoxigenin - immunology Digoxin Digoxin - analysis Digoxin - immunology Exact sciences and technology Fluorescence quenching Fluorescent Dyes - chemistry Gold - chemistry Gold nanoparticle Immunoassay - methods Metal Nanoparticles - chemistry Miscellaneous Serum Albumin, Bovine - chemistry Serum Albumin, Bovine - immunology Spectrometric and optical methods Spectrophotometry, Ultraviolet
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!