Validated liquid chromatographic ultraviolet method for the quantitation of Etoricoxib in human plasma using liquid–liquid extraction
A simple, sensitive and specific HPLC method with UV detection (284 nm) was developed and validated for quantitation of Etoricoxib in human plasma, the newest addition to the group of nonsteroidal anti-inflammatory drugs—a highly selective cyclooxygenase-2 inhibitor. Following a single-step liquid–l...
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| Veröffentlicht in: | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2005-02, Vol.816 (1), p.215-221 |
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| container_title | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences |
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| creator | Ramakrishna, N.V.S. Vishwottam, K.N. Wishu, S. Koteshwara, M. |
| description | A simple, sensitive and specific HPLC method with UV detection (284
nm) was developed and validated for quantitation of Etoricoxib in human plasma, the newest addition to the group of nonsteroidal anti-inflammatory drugs—a highly selective cyclooxygenase-2 inhibitor. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30, v/v), the analyte and internal standard (Zaleplon) were separated using an isocratic mobile phase of water/acetonitrile (58/42, v/v) on reverse phase Waters symmetry
® C
18 column. The lower limit of quantitation was 5
ng/mL, with a relative standard deviation of less than 20%. A linear range of 5–2500
ng/mL was established. This HPLC method was validated with between- and within-batch precision of 4.1–5.1% and 1.1–2.4%, respectively. The between- and within-batch bias was −3.8–4.7% and −0.6–9.4%, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of Etoricoxib in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of |
| doi_str_mv | 10.1016/j.jchromb.2004.11.034 |
| format | Article |
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nm) was developed and validated for quantitation of Etoricoxib in human plasma, the newest addition to the group of nonsteroidal anti-inflammatory drugs—a highly selective cyclooxygenase-2 inhibitor. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30, v/v), the analyte and internal standard (Zaleplon) were separated using an isocratic mobile phase of water/acetonitrile (58/42, v/v) on reverse phase Waters symmetry
® C
18 column. The lower limit of quantitation was 5
ng/mL, with a relative standard deviation of less than 20%. A linear range of 5–2500
ng/mL was established. This HPLC method was validated with between- and within-batch precision of 4.1–5.1% and 1.1–2.4%, respectively. The between- and within-batch bias was −3.8–4.7% and −0.6–9.4%, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of Etoricoxib in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <6% and was used in pharmacokinetic studies.</description><identifier>ISSN: 1570-0232</identifier><identifier>EISSN: 1873-376X</identifier><identifier>DOI: 10.1016/j.jchromb.2004.11.034</identifier><identifier>PMID: 15664353</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Analysis ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Chromatography, High Pressure Liquid - methods ; Cyclooxygenase Inhibitors - blood ; Drug Stability ; Etoricoxib ; Fundamental and applied biological sciences. Psychology ; General pharmacology ; Human plasma ; Humans ; Liquid–liquid extraction ; Medical sciences ; Pharmacology. Drug treatments ; Pyridines - blood ; Pyridines - isolation & purification ; Pyridines - pharmacokinetics ; Quantitation ; Reproducibility of Results ; Sensitivity and Specificity ; Sulfones - blood ; Sulfones - isolation & purification ; Sulfones - pharmacokinetics</subject><ispartof>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-02, Vol.816 (1), p.215-221</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c422t-bc1bff0be0c47e2331588cc792f39305806e733d22267f212e7948603027c2183</citedby><cites>FETCH-LOGICAL-c422t-bc1bff0be0c47e2331588cc792f39305806e733d22267f212e7948603027c2183</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jchromb.2004.11.034$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3548,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16451378$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15664353$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ramakrishna, N.V.S.</creatorcontrib><creatorcontrib>Vishwottam, K.N.</creatorcontrib><creatorcontrib>Wishu, S.</creatorcontrib><creatorcontrib>Koteshwara, M.</creatorcontrib><title>Validated liquid chromatographic ultraviolet method for the quantitation of Etoricoxib in human plasma using liquid–liquid extraction</title><title>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</title><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><description>A simple, sensitive and specific HPLC method with UV detection (284
nm) was developed and validated for quantitation of Etoricoxib in human plasma, the newest addition to the group of nonsteroidal anti-inflammatory drugs—a highly selective cyclooxygenase-2 inhibitor. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30, v/v), the analyte and internal standard (Zaleplon) were separated using an isocratic mobile phase of water/acetonitrile (58/42, v/v) on reverse phase Waters symmetry
® C
18 column. The lower limit of quantitation was 5
ng/mL, with a relative standard deviation of less than 20%. A linear range of 5–2500
ng/mL was established. This HPLC method was validated with between- and within-batch precision of 4.1–5.1% and 1.1–2.4%, respectively. The between- and within-batch bias was −3.8–4.7% and −0.6–9.4%, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of Etoricoxib in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <6% and was used in pharmacokinetic studies.</description><subject>Analysis</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Chromatography, High Pressure Liquid - methods</subject><subject>Cyclooxygenase Inhibitors - blood</subject><subject>Drug Stability</subject><subject>Etoricoxib</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General pharmacology</subject><subject>Human plasma</subject><subject>Humans</subject><subject>Liquid–liquid extraction</subject><subject>Medical sciences</subject><subject>Pharmacology. Drug treatments</subject><subject>Pyridines - blood</subject><subject>Pyridines - isolation & purification</subject><subject>Pyridines - pharmacokinetics</subject><subject>Quantitation</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Sulfones - blood</subject><subject>Sulfones - isolation & purification</subject><subject>Sulfones - pharmacokinetics</subject><issn>1570-0232</issn><issn>1873-376X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc1u1DAUhS0Eou3AI4C8gV2CfxI7s0KoKhSpEhtA7CzHuWnuyIlnbKdqd-x4AN6QJyHDROqS1b2L75xzdQ8hrzgrOePq3a7cuSGGsS0FY1XJeclk9YSc80bLQmr14-my15oVTEhxRi5S2jHGNdPyOTnjtVKVrOU5-fXdeuxsho56PMzY0X-uNofbaPcDOjr7HO0dBg-ZjpCH0NE-RJoHoIfZThmzzRgmGnp6lUNEF-6xpTjRYR7tRPfeptHSOeF0u0b8-fl7zYL7xdsd5S_Is976BC_XuSHfPl59vbwubr58-nz54aZwlRC5aB1v-561wFylQUjJ66ZxTm9FL7eS1Q1ToKXshBBK94IL0NuqUUwyoZ3gjdyQtyfffQyHGVI2IyYH3tsJwpyM0lKL7WK8IfUJdDGkFKE3-4ijjQ-GM3NswOzM2oA5NmA4N0sDi-71GjC3I3SPqvXlC_BmBWxy1vfRTg7TI6eqmkt9vPT9iYPlHXcI0SSHMDnoMILLpgv4n1P-Ao_qqo0</recordid><startdate>20050225</startdate><enddate>20050225</enddate><creator>Ramakrishna, N.V.S.</creator><creator>Vishwottam, K.N.</creator><creator>Wishu, S.</creator><creator>Koteshwara, M.</creator><general>Elsevier B.V</general><general>Elsevier Science</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050225</creationdate><title>Validated liquid chromatographic ultraviolet method for the quantitation of Etoricoxib in human plasma using liquid–liquid extraction</title><author>Ramakrishna, N.V.S. ; Vishwottam, K.N. ; Wishu, S. ; Koteshwara, M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c422t-bc1bff0be0c47e2331588cc792f39305806e733d22267f212e7948603027c2183</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Analysis</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Chromatography, High Pressure Liquid - methods</topic><topic>Cyclooxygenase Inhibitors - blood</topic><topic>Drug Stability</topic><topic>Etoricoxib</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General pharmacology</topic><topic>Human plasma</topic><topic>Humans</topic><topic>Liquid–liquid extraction</topic><topic>Medical sciences</topic><topic>Pharmacology. Drug treatments</topic><topic>Pyridines - blood</topic><topic>Pyridines - isolation & purification</topic><topic>Pyridines - pharmacokinetics</topic><topic>Quantitation</topic><topic>Reproducibility of Results</topic><topic>Sensitivity and Specificity</topic><topic>Sulfones - blood</topic><topic>Sulfones - isolation & purification</topic><topic>Sulfones - pharmacokinetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ramakrishna, N.V.S.</creatorcontrib><creatorcontrib>Vishwottam, K.N.</creatorcontrib><creatorcontrib>Wishu, S.</creatorcontrib><creatorcontrib>Koteshwara, M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ramakrishna, N.V.S.</au><au>Vishwottam, K.N.</au><au>Wishu, S.</au><au>Koteshwara, M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Validated liquid chromatographic ultraviolet method for the quantitation of Etoricoxib in human plasma using liquid–liquid extraction</atitle><jtitle>Journal of chromatography. B, Analytical technologies in the biomedical and life sciences</jtitle><addtitle>J Chromatogr B Analyt Technol Biomed Life Sci</addtitle><date>2005-02-25</date><risdate>2005</risdate><volume>816</volume><issue>1</issue><spage>215</spage><epage>221</epage><pages>215-221</pages><issn>1570-0232</issn><eissn>1873-376X</eissn><abstract>A simple, sensitive and specific HPLC method with UV detection (284
nm) was developed and validated for quantitation of Etoricoxib in human plasma, the newest addition to the group of nonsteroidal anti-inflammatory drugs—a highly selective cyclooxygenase-2 inhibitor. Following a single-step liquid–liquid extraction with diethyl ether/dichloromethane (70/30, v/v), the analyte and internal standard (Zaleplon) were separated using an isocratic mobile phase of water/acetonitrile (58/42, v/v) on reverse phase Waters symmetry
® C
18 column. The lower limit of quantitation was 5
ng/mL, with a relative standard deviation of less than 20%. A linear range of 5–2500
ng/mL was established. This HPLC method was validated with between- and within-batch precision of 4.1–5.1% and 1.1–2.4%, respectively. The between- and within-batch bias was −3.8–4.7% and −0.6–9.4%, respectively. Frequently coadministered drugs did not interfere with the described methodology. Stability of Etoricoxib in plasma was >90%, with no evidence of degradation during sample processing (autosampler) and 30 days storage in a freezer. This validated method is sensitive and simple with between-batch precision of <6% and was used in pharmacokinetic studies.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15664353</pmid><doi>10.1016/j.jchromb.2004.11.034</doi><tpages>7</tpages></addata></record> |
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| ispartof | Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 2005-02, Vol.816 (1), p.215-221 |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Analysis Analytical, structural and metabolic biochemistry Biological and medical sciences Chromatography, High Pressure Liquid - methods Cyclooxygenase Inhibitors - blood Drug Stability Etoricoxib Fundamental and applied biological sciences. Psychology General pharmacology Human plasma Humans Liquid–liquid extraction Medical sciences Pharmacology. Drug treatments Pyridines - blood Pyridines - isolation & purification Pyridines - pharmacokinetics Quantitation Reproducibility of Results Sensitivity and Specificity Sulfones - blood Sulfones - isolation & purification Sulfones - pharmacokinetics |
| title | Validated liquid chromatographic ultraviolet method for the quantitation of Etoricoxib in human plasma using liquid–liquid extraction |
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