The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner
Morphological changes at synaptic specializations have been implicated in regulating synaptic strength. Actin turnover at dendritic spines is regulated by neuronal activity and contributes to spine size, shape and motility. The reorganization of actin filaments requires profilins, which stimulate ac...
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creator | Neuhoff, Henrike Sassoè-Pognetto, Marco Panzanelli, Patrizia Maas, Christoph Witke, Walter Kneussel, Matthias |
description | Morphological changes at synaptic specializations have been implicated in regulating synaptic strength. Actin turnover at dendritic spines is regulated by neuronal activity and contributes to spine size, shape and motility. The reorganization of actin filaments requires profilins, which stimulate actin polymerization. Neurons express two independent gene products − profilin I and profilin II. A role for profilin II in activity‐dependent mechanisms at spine synapses has recently been described. Although profilin I interacts with synaptic proteins, little is known about its cellular and subcellular localization in neurons. Here, we investigated the subcellular distribution of this protein in brain neurons as well as in hippocampal cultures. Our results indicate that the expression of profilin I varies in different brain regions. Thus, in cerebral cortex and hippocampus profilin I immunostaining was associated predominantly with dendrites and was present in a subset of dendritic spines. In contrast, profilin I in cerebellum was associated primarily with presynaptic structures. Profilin I immunoreactivity was partially colocalized with the synaptic molecules synaptophysin, PSD‐95 and gephyrin in cultured hippocampal neurons, indicating that profilin I is present in only a subset of synapses. At dendritic spine structures, profilin I was found primarily in protrusions, which were in apposition to presynaptic terminal boutons. Remarkably, depolarization with KCl caused a moderate but significant increase in the number of synapses containing profilin I. These results show that profilin I can be present at both pre‐ and postsynaptic sites and suggest a role for this actin‐binding protein in activity‐dependent remodelling of synaptic structure. |
doi_str_mv | 10.1111/j.1460-9568.2004.03814.x |
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Actin turnover at dendritic spines is regulated by neuronal activity and contributes to spine size, shape and motility. The reorganization of actin filaments requires profilins, which stimulate actin polymerization. Neurons express two independent gene products − profilin I and profilin II. A role for profilin II in activity‐dependent mechanisms at spine synapses has recently been described. Although profilin I interacts with synaptic proteins, little is known about its cellular and subcellular localization in neurons. Here, we investigated the subcellular distribution of this protein in brain neurons as well as in hippocampal cultures. Our results indicate that the expression of profilin I varies in different brain regions. Thus, in cerebral cortex and hippocampus profilin I immunostaining was associated predominantly with dendrites and was present in a subset of dendritic spines. In contrast, profilin I in cerebellum was associated primarily with presynaptic structures. Profilin I immunoreactivity was partially colocalized with the synaptic molecules synaptophysin, PSD‐95 and gephyrin in cultured hippocampal neurons, indicating that profilin I is present in only a subset of synapses. At dendritic spine structures, profilin I was found primarily in protrusions, which were in apposition to presynaptic terminal boutons. Remarkably, depolarization with KCl caused a moderate but significant increase in the number of synapses containing profilin I. These results show that profilin I can be present at both pre‐ and postsynaptic sites and suggest a role for this actin‐binding protein in activity‐dependent remodelling of synaptic structure.</description><identifier>ISSN: 0953-816X</identifier><identifier>EISSN: 1460-9568</identifier><identifier>DOI: 10.1111/j.1460-9568.2004.03814.x</identifier><identifier>PMID: 15654839</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>actin ; activity-dependent ; Adaptor Proteins, Signal Transducing - metabolism ; alpha Catenin ; Animals ; Animals, Newborn ; Brain - anatomy & histology ; Brain - metabolism ; Carrier Proteins - metabolism ; Cell Count - methods ; Cells, Cultured ; Contractile Proteins - metabolism ; Cytoskeletal Proteins - metabolism ; dendritic spine ; Dendritic Spines - drug effects ; Dendritic Spines - metabolism ; Dendritic Spines - ultrastructure ; Disks Large Homolog 4 Protein ; Gene Expression Regulation - drug effects ; Gene Expression Regulation - physiology ; Green Fluorescent Proteins - metabolism ; Guanylate Kinases ; Hippocampus - cytology ; Hippocampus - metabolism ; Immunohistochemistry - methods ; Intracellular Signaling Peptides and Proteins ; Membrane Proteins - metabolism ; Mice ; Mice, Inbred C57BL ; Microfilament Proteins - metabolism ; Microscopy, Immunoelectron - methods ; Nerve Tissue Proteins - metabolism ; Neuronal Plasticity - drug effects ; Neuronal Plasticity - physiology ; Neurons - cytology ; Neurons - drug effects ; Neurons - metabolism ; Potassium Chloride - pharmacology ; profilin ; Profilins ; Statistics, Nonparametric ; synapse ; Synapses - drug effects ; Synapses - metabolism ; Synapses - ultrastructure ; Synaptophysin - metabolism</subject><ispartof>The European journal of neuroscience, 2005-01, Vol.21 (1), p.15-25</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5024-c08beaff8d16e814600ec4a15ddd712fd45a1c6d5a44830e8e56affa9aaafcd63</citedby><cites>FETCH-LOGICAL-c5024-c08beaff8d16e814600ec4a15ddd712fd45a1c6d5a44830e8e56affa9aaafcd63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1460-9568.2004.03814.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1460-9568.2004.03814.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27903,27904,45553,45554</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15654839$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Neuhoff, Henrike</creatorcontrib><creatorcontrib>Sassoè-Pognetto, Marco</creatorcontrib><creatorcontrib>Panzanelli, Patrizia</creatorcontrib><creatorcontrib>Maas, Christoph</creatorcontrib><creatorcontrib>Witke, Walter</creatorcontrib><creatorcontrib>Kneussel, Matthias</creatorcontrib><title>The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner</title><title>The European journal of neuroscience</title><addtitle>Eur J Neurosci</addtitle><description>Morphological changes at synaptic specializations have been implicated in regulating synaptic strength. Actin turnover at dendritic spines is regulated by neuronal activity and contributes to spine size, shape and motility. The reorganization of actin filaments requires profilins, which stimulate actin polymerization. Neurons express two independent gene products − profilin I and profilin II. A role for profilin II in activity‐dependent mechanisms at spine synapses has recently been described. Although profilin I interacts with synaptic proteins, little is known about its cellular and subcellular localization in neurons. Here, we investigated the subcellular distribution of this protein in brain neurons as well as in hippocampal cultures. Our results indicate that the expression of profilin I varies in different brain regions. Thus, in cerebral cortex and hippocampus profilin I immunostaining was associated predominantly with dendrites and was present in a subset of dendritic spines. In contrast, profilin I in cerebellum was associated primarily with presynaptic structures. Profilin I immunoreactivity was partially colocalized with the synaptic molecules synaptophysin, PSD‐95 and gephyrin in cultured hippocampal neurons, indicating that profilin I is present in only a subset of synapses. At dendritic spine structures, profilin I was found primarily in protrusions, which were in apposition to presynaptic terminal boutons. Remarkably, depolarization with KCl caused a moderate but significant increase in the number of synapses containing profilin I. These results show that profilin I can be present at both pre‐ and postsynaptic sites and suggest a role for this actin‐binding protein in activity‐dependent remodelling of synaptic structure.</description><subject>actin</subject><subject>activity-dependent</subject><subject>Adaptor Proteins, Signal Transducing - metabolism</subject><subject>alpha Catenin</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Brain - anatomy & histology</subject><subject>Brain - metabolism</subject><subject>Carrier Proteins - metabolism</subject><subject>Cell Count - methods</subject><subject>Cells, Cultured</subject><subject>Contractile Proteins - metabolism</subject><subject>Cytoskeletal Proteins - metabolism</subject><subject>dendritic spine</subject><subject>Dendritic Spines - drug effects</subject><subject>Dendritic Spines - metabolism</subject><subject>Dendritic Spines - ultrastructure</subject><subject>Disks Large Homolog 4 Protein</subject><subject>Gene Expression Regulation - drug effects</subject><subject>Gene Expression Regulation - physiology</subject><subject>Green Fluorescent Proteins - metabolism</subject><subject>Guanylate Kinases</subject><subject>Hippocampus - cytology</subject><subject>Hippocampus - metabolism</subject><subject>Immunohistochemistry - methods</subject><subject>Intracellular Signaling Peptides and Proteins</subject><subject>Membrane Proteins - metabolism</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Microfilament Proteins - metabolism</subject><subject>Microscopy, Immunoelectron - methods</subject><subject>Nerve Tissue Proteins - metabolism</subject><subject>Neuronal Plasticity - drug effects</subject><subject>Neuronal Plasticity - physiology</subject><subject>Neurons - cytology</subject><subject>Neurons - drug effects</subject><subject>Neurons - metabolism</subject><subject>Potassium Chloride - pharmacology</subject><subject>profilin</subject><subject>Profilins</subject><subject>Statistics, Nonparametric</subject><subject>synapse</subject><subject>Synapses - drug effects</subject><subject>Synapses - metabolism</subject><subject>Synapses - ultrastructure</subject><subject>Synaptophysin - metabolism</subject><issn>0953-816X</issn><issn>1460-9568</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkcFuEzEQhi0EomnhFZBP3HZr79qO98ABqtIWhVYqhSAu1sSeLQ4bJ6ydkvD0eJOoHMGXGcnfP_PPDCGUs5LndzovuVCsaKTSZcWYKFmtuSg3T8jo8eMpGbFG1oXm6usROY5xzhjTSsjn5IhLJYWumxGxd9-Rgk0-FDMfnA_3dNUvE_owxNZ3ObmiPtJuaaHzv9FRSDRuA6yStzT6hJFmBsKuyoNP26LH-3UHKaMLCAH7F-RZC13El4d4Qj6_P787uywmNxdXZ28nhZWsEoVleobQttpxhXqYg6EVwKVzbsyr1gkJ3ConQWTvDDVKlXFoAKC1TtUn5PW-bnb-c40xmYWPFrsOAi7X0ahxPa64av4J8nEt8rKqDOo9aPtljD22ZtX7BfRbw5kZLmHmZjBqhoWb4RJmdwmzydJXhx7r2QLdX-Fh9Rl4swd--Q63_13YnH-4HrKsL_Z6HxNuHvXQ_9gNKs30-sLcTj5Op5_efTHf6j-Ty6jR</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>Neuhoff, Henrike</creator><creator>Sassoè-Pognetto, Marco</creator><creator>Panzanelli, Patrizia</creator><creator>Maas, Christoph</creator><creator>Witke, Walter</creator><creator>Kneussel, Matthias</creator><general>Blackwell Science Ltd</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner</title><author>Neuhoff, Henrike ; Sassoè-Pognetto, Marco ; Panzanelli, Patrizia ; Maas, Christoph ; Witke, Walter ; Kneussel, Matthias</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5024-c08beaff8d16e814600ec4a15ddd712fd45a1c6d5a44830e8e56affa9aaafcd63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>actin</topic><topic>activity-dependent</topic><topic>Adaptor Proteins, Signal Transducing - metabolism</topic><topic>alpha Catenin</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Brain - anatomy & histology</topic><topic>Brain - metabolism</topic><topic>Carrier Proteins - metabolism</topic><topic>Cell Count - methods</topic><topic>Cells, Cultured</topic><topic>Contractile Proteins - metabolism</topic><topic>Cytoskeletal Proteins - metabolism</topic><topic>dendritic spine</topic><topic>Dendritic Spines - drug effects</topic><topic>Dendritic Spines - metabolism</topic><topic>Dendritic Spines - ultrastructure</topic><topic>Disks Large Homolog 4 Protein</topic><topic>Gene Expression Regulation - drug effects</topic><topic>Gene Expression Regulation - physiology</topic><topic>Green Fluorescent Proteins - metabolism</topic><topic>Guanylate Kinases</topic><topic>Hippocampus - cytology</topic><topic>Hippocampus - metabolism</topic><topic>Immunohistochemistry - methods</topic><topic>Intracellular Signaling Peptides and Proteins</topic><topic>Membrane Proteins - metabolism</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Microfilament Proteins - metabolism</topic><topic>Microscopy, Immunoelectron - methods</topic><topic>Nerve Tissue Proteins - metabolism</topic><topic>Neuronal Plasticity - drug effects</topic><topic>Neuronal Plasticity - physiology</topic><topic>Neurons - cytology</topic><topic>Neurons - drug effects</topic><topic>Neurons - metabolism</topic><topic>Potassium Chloride - pharmacology</topic><topic>profilin</topic><topic>Profilins</topic><topic>Statistics, Nonparametric</topic><topic>synapse</topic><topic>Synapses - drug effects</topic><topic>Synapses - metabolism</topic><topic>Synapses - ultrastructure</topic><topic>Synaptophysin - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Neuhoff, Henrike</creatorcontrib><creatorcontrib>Sassoè-Pognetto, Marco</creatorcontrib><creatorcontrib>Panzanelli, Patrizia</creatorcontrib><creatorcontrib>Maas, Christoph</creatorcontrib><creatorcontrib>Witke, Walter</creatorcontrib><creatorcontrib>Kneussel, Matthias</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>The European journal of neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Neuhoff, Henrike</au><au>Sassoè-Pognetto, Marco</au><au>Panzanelli, Patrizia</au><au>Maas, Christoph</au><au>Witke, Walter</au><au>Kneussel, Matthias</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner</atitle><jtitle>The European journal of neuroscience</jtitle><addtitle>Eur J Neurosci</addtitle><date>2005-01</date><risdate>2005</risdate><volume>21</volume><issue>1</issue><spage>15</spage><epage>25</epage><pages>15-25</pages><issn>0953-816X</issn><eissn>1460-9568</eissn><abstract>Morphological changes at synaptic specializations have been implicated in regulating synaptic strength. Actin turnover at dendritic spines is regulated by neuronal activity and contributes to spine size, shape and motility. The reorganization of actin filaments requires profilins, which stimulate actin polymerization. Neurons express two independent gene products − profilin I and profilin II. A role for profilin II in activity‐dependent mechanisms at spine synapses has recently been described. Although profilin I interacts with synaptic proteins, little is known about its cellular and subcellular localization in neurons. Here, we investigated the subcellular distribution of this protein in brain neurons as well as in hippocampal cultures. Our results indicate that the expression of profilin I varies in different brain regions. Thus, in cerebral cortex and hippocampus profilin I immunostaining was associated predominantly with dendrites and was present in a subset of dendritic spines. In contrast, profilin I in cerebellum was associated primarily with presynaptic structures. Profilin I immunoreactivity was partially colocalized with the synaptic molecules synaptophysin, PSD‐95 and gephyrin in cultured hippocampal neurons, indicating that profilin I is present in only a subset of synapses. At dendritic spine structures, profilin I was found primarily in protrusions, which were in apposition to presynaptic terminal boutons. Remarkably, depolarization with KCl caused a moderate but significant increase in the number of synapses containing profilin I. These results show that profilin I can be present at both pre‐ and postsynaptic sites and suggest a role for this actin‐binding protein in activity‐dependent remodelling of synaptic structure.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15654839</pmid><doi>10.1111/j.1460-9568.2004.03814.x</doi><tpages>11</tpages></addata></record> |
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subjects | actin activity-dependent Adaptor Proteins, Signal Transducing - metabolism alpha Catenin Animals Animals, Newborn Brain - anatomy & histology Brain - metabolism Carrier Proteins - metabolism Cell Count - methods Cells, Cultured Contractile Proteins - metabolism Cytoskeletal Proteins - metabolism dendritic spine Dendritic Spines - drug effects Dendritic Spines - metabolism Dendritic Spines - ultrastructure Disks Large Homolog 4 Protein Gene Expression Regulation - drug effects Gene Expression Regulation - physiology Green Fluorescent Proteins - metabolism Guanylate Kinases Hippocampus - cytology Hippocampus - metabolism Immunohistochemistry - methods Intracellular Signaling Peptides and Proteins Membrane Proteins - metabolism Mice Mice, Inbred C57BL Microfilament Proteins - metabolism Microscopy, Immunoelectron - methods Nerve Tissue Proteins - metabolism Neuronal Plasticity - drug effects Neuronal Plasticity - physiology Neurons - cytology Neurons - drug effects Neurons - metabolism Potassium Chloride - pharmacology profilin Profilins Statistics, Nonparametric synapse Synapses - drug effects Synapses - metabolism Synapses - ultrastructure Synaptophysin - metabolism |
title | The actin-binding protein profilin I is localized at synaptic sites in an activity-regulated manner |
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