PCR Testing of Pooled Longitudinally Collected Cervical Specimens of Women to Increase the Efficiency of Studying Human Papillomavirus Infection
In large active cohort studies of women investigating human papillomavirus (HPV) and cervical neoplasia, many women will be HPV-negative at all time points and testing of all their cervical specimens is an inefficient use of laboratory resources. The aim of this pilot study was to evaluate whether p...
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| Veröffentlicht in: | Cancer epidemiology, biomarkers & prevention biomarkers & prevention, 2005-01, Vol.14 (1), p.256-260 |
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| creator | CASTLE, Philip E SCHIFFMAN, Mark PFEIFFER, Ruth BURK, Robert D HERRERO, Rolando HILDESHEIM, Allan RODRIGUEZ, Ana-Cecilia BRATTI, M. Concepcion WACHOLDER, Sholom KENDAL, Hortense BREHENY, Anne M PRIOR, Andrew |
| description | In large active cohort studies of women investigating human papillomavirus (HPV) and cervical neoplasia, many women will be
HPV-negative at all time points and testing of all their cervical specimens is an inefficient use of laboratory resources.
The aim of this pilot study was to evaluate whether pooling cervical specimens from the same woman might provide a useful
pretest of specimens from women unlikely to have high-grade cervical neoplasia or significant HPV exposure. We selected women
( n = 187) participating in the Guanacaste Project for whom we already had HPV testing data on all their specimens from multiple
visits (median = 8 visits), who were HPV DNA-negative at enrollment and at their 5- to 7-year exit from the cohort, and had
no evidence of high-grade cervical neoplasia. Equal aliquots of cervical specimens from these women were pooled to create
a proportional pooled specimen. Aliquots of pooled specimens were tested in a masked fashion by MY09/11 L1 consensus primer
PCR. Second aliquots of some pooled specimens ( n = 83) were included to assess the reliability of pooled testing. Results were compared with the predicted (expected) results
based on the obtained test results of the individual specimens collected at interim visits. There was good overall agreement
between observed and expected HPV DNA positivity, with a κ of 0.63 [95% confidence interval (95% CI), 0.51-0.75] and a percent agreement of 83.4% (95% CI, 77.3-88.5%) although the
HPV DNA positivity in the pooled specimen was less than expected ( P = 0.001). The agreement between observed and expected HPV DNA positivity was related to the number of aliquots pooled, suggesting
that positivity was related to viral genome concentrations. The κ and percent agreement for intra-batch reliability of testing pooled specimens were 0.68 (95% CI, 0.53-0.84) and 84.3% (95%
CI, 74.7-91.4%), respectively. We conclude that pooling specimens and testing by PCR may be useful for discriminating HPV
DNA-positive from completely negative specimen sets in women who are likely to have been HPV DNA-negative.> |
| doi_str_mv | 10.1158/1055-9965.256.14.1 |
| format | Article |
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HPV-negative at all time points and testing of all their cervical specimens is an inefficient use of laboratory resources.
The aim of this pilot study was to evaluate whether pooling cervical specimens from the same woman might provide a useful
pretest of specimens from women unlikely to have high-grade cervical neoplasia or significant HPV exposure. We selected women
( n = 187) participating in the Guanacaste Project for whom we already had HPV testing data on all their specimens from multiple
visits (median = 8 visits), who were HPV DNA-negative at enrollment and at their 5- to 7-year exit from the cohort, and had
no evidence of high-grade cervical neoplasia. Equal aliquots of cervical specimens from these women were pooled to create
a proportional pooled specimen. Aliquots of pooled specimens were tested in a masked fashion by MY09/11 L1 consensus primer
PCR. Second aliquots of some pooled specimens ( n = 83) were included to assess the reliability of pooled testing. Results were compared with the predicted (expected) results
based on the obtained test results of the individual specimens collected at interim visits. There was good overall agreement
between observed and expected HPV DNA positivity, with a κ of 0.63 [95% confidence interval (95% CI), 0.51-0.75] and a percent agreement of 83.4% (95% CI, 77.3-88.5%) although the
HPV DNA positivity in the pooled specimen was less than expected ( P = 0.001). The agreement between observed and expected HPV DNA positivity was related to the number of aliquots pooled, suggesting
that positivity was related to viral genome concentrations. The κ and percent agreement for intra-batch reliability of testing pooled specimens were 0.68 (95% CI, 0.53-0.84) and 84.3% (95%
CI, 74.7-91.4%), respectively. We conclude that pooling specimens and testing by PCR may be useful for discriminating HPV
DNA-positive from completely negative specimen sets in women who are likely to have been HPV DNA-negative.></description><identifier>ISSN: 1055-9965</identifier><identifier>EISSN: 1538-7755</identifier><identifier>DOI: 10.1158/1055-9965.256.14.1</identifier><identifier>PMID: 15668503</identifier><language>eng</language><publisher>Philadelphia, PA: American Association for Cancer Research</publisher><subject>Biological and medical sciences ; Cervical Intraepithelial Neoplasia - virology ; Female ; Female genital diseases ; Gynecology. Andrology. Obstetrics ; Human papillomavirus ; Humans ; Longitudinal Studies ; Medical sciences ; Papillomaviridae - isolation & purification ; Papillomavirus Infections - complications ; Papillomavirus Infections - diagnosis ; Pilot Projects ; Polymerase Chain Reaction - methods ; Reproducibility of Results ; Tumors ; Uterine Cervical Neoplasms - virology</subject><ispartof>Cancer epidemiology, biomarkers & prevention, 2005-01, Vol.14 (1), p.256-260</ispartof><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c438t-8238e6a68707a7b75bf4c399eb2513cb2deecce3b876ebaf03608a115a4a67b93</citedby><cites>FETCH-LOGICAL-c438t-8238e6a68707a7b75bf4c399eb2513cb2deecce3b876ebaf03608a115a4a67b93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,3343,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16438831$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15668503$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>CASTLE, Philip E</creatorcontrib><creatorcontrib>SCHIFFMAN, Mark</creatorcontrib><creatorcontrib>PFEIFFER, Ruth</creatorcontrib><creatorcontrib>BURK, Robert D</creatorcontrib><creatorcontrib>HERRERO, Rolando</creatorcontrib><creatorcontrib>HILDESHEIM, Allan</creatorcontrib><creatorcontrib>RODRIGUEZ, Ana-Cecilia</creatorcontrib><creatorcontrib>BRATTI, M. Concepcion</creatorcontrib><creatorcontrib>WACHOLDER, Sholom</creatorcontrib><creatorcontrib>KENDAL, Hortense</creatorcontrib><creatorcontrib>BREHENY, Anne M</creatorcontrib><creatorcontrib>PRIOR, Andrew</creatorcontrib><title>PCR Testing of Pooled Longitudinally Collected Cervical Specimens of Women to Increase the Efficiency of Studying Human Papillomavirus Infection</title><title>Cancer epidemiology, biomarkers & prevention</title><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><description>In large active cohort studies of women investigating human papillomavirus (HPV) and cervical neoplasia, many women will be
HPV-negative at all time points and testing of all their cervical specimens is an inefficient use of laboratory resources.
The aim of this pilot study was to evaluate whether pooling cervical specimens from the same woman might provide a useful
pretest of specimens from women unlikely to have high-grade cervical neoplasia or significant HPV exposure. We selected women
( n = 187) participating in the Guanacaste Project for whom we already had HPV testing data on all their specimens from multiple
visits (median = 8 visits), who were HPV DNA-negative at enrollment and at their 5- to 7-year exit from the cohort, and had
no evidence of high-grade cervical neoplasia. Equal aliquots of cervical specimens from these women were pooled to create
a proportional pooled specimen. Aliquots of pooled specimens were tested in a masked fashion by MY09/11 L1 consensus primer
PCR. Second aliquots of some pooled specimens ( n = 83) were included to assess the reliability of pooled testing. Results were compared with the predicted (expected) results
based on the obtained test results of the individual specimens collected at interim visits. There was good overall agreement
between observed and expected HPV DNA positivity, with a κ of 0.63 [95% confidence interval (95% CI), 0.51-0.75] and a percent agreement of 83.4% (95% CI, 77.3-88.5%) although the
HPV DNA positivity in the pooled specimen was less than expected ( P = 0.001). The agreement between observed and expected HPV DNA positivity was related to the number of aliquots pooled, suggesting
that positivity was related to viral genome concentrations. The κ and percent agreement for intra-batch reliability of testing pooled specimens were 0.68 (95% CI, 0.53-0.84) and 84.3% (95%
CI, 74.7-91.4%), respectively. We conclude that pooling specimens and testing by PCR may be useful for discriminating HPV
DNA-positive from completely negative specimen sets in women who are likely to have been HPV DNA-negative.></description><subject>Biological and medical sciences</subject><subject>Cervical Intraepithelial Neoplasia - virology</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Human papillomavirus</subject><subject>Humans</subject><subject>Longitudinal Studies</subject><subject>Medical sciences</subject><subject>Papillomaviridae - isolation & purification</subject><subject>Papillomavirus Infections - complications</subject><subject>Papillomavirus Infections - diagnosis</subject><subject>Pilot Projects</subject><subject>Polymerase Chain Reaction - methods</subject><subject>Reproducibility of Results</subject><subject>Tumors</subject><subject>Uterine Cervical Neoplasms - virology</subject><issn>1055-9965</issn><issn>1538-7755</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9u1DAYxC0EoqXwAhyQL8Apix3Hf3JEUaGVVmJFizhajvfLrpFjL3ZStG_BI-Owi3rk5E_yb2akGYReU7KilKsPlHBeta3gq5qLFW1W9Am6pJypSkrOn5b7H3CBXuT8gxAiW86fowvKhVCcsEv0e9N9xfeQJxd2OA54E6OHLV7HsHPTvHXBeH_EXfQe7FQ-OkgPzhqP7w5g3QghL6rvsVx4ivg22AQmA572gK-HwVkHwR4X5q7YHZeUm3k0AW_MwXkfR_Pg0pyLcCgBLoaX6NlgfIZX5_cKfft0fd_dVOsvn2-7j-vKNkxNlaqZAmGEkkQa2UveD41lbQt9zSmzfb0FsBZYr6SA3gyECaJMqc00Rsi-ZVfo3cn3kOLPuRSgR5cteG8CxDlrIZmksnT0P5BKRRiRooD1CbQp5pxg0IfkRpOOmhK9DKaXPfSyhy6DadpoWkRvzu5zP8L2UXJeqABvz4DJpfchmWBdfuREqUOxxej9idu73f6XS6BtISElyGCS3f-NW3LZH2AHraw</recordid><startdate>20050101</startdate><enddate>20050101</enddate><creator>CASTLE, Philip E</creator><creator>SCHIFFMAN, Mark</creator><creator>PFEIFFER, Ruth</creator><creator>BURK, Robert D</creator><creator>HERRERO, Rolando</creator><creator>HILDESHEIM, Allan</creator><creator>RODRIGUEZ, Ana-Cecilia</creator><creator>BRATTI, M. 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Obstetrics</topic><topic>Human papillomavirus</topic><topic>Humans</topic><topic>Longitudinal Studies</topic><topic>Medical sciences</topic><topic>Papillomaviridae - isolation & purification</topic><topic>Papillomavirus Infections - complications</topic><topic>Papillomavirus Infections - diagnosis</topic><topic>Pilot Projects</topic><topic>Polymerase Chain Reaction - methods</topic><topic>Reproducibility of Results</topic><topic>Tumors</topic><topic>Uterine Cervical Neoplasms - virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>CASTLE, Philip E</creatorcontrib><creatorcontrib>SCHIFFMAN, Mark</creatorcontrib><creatorcontrib>PFEIFFER, Ruth</creatorcontrib><creatorcontrib>BURK, Robert D</creatorcontrib><creatorcontrib>HERRERO, Rolando</creatorcontrib><creatorcontrib>HILDESHEIM, Allan</creatorcontrib><creatorcontrib>RODRIGUEZ, Ana-Cecilia</creatorcontrib><creatorcontrib>BRATTI, M. 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Concepcion</au><au>WACHOLDER, Sholom</au><au>KENDAL, Hortense</au><au>BREHENY, Anne M</au><au>PRIOR, Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>PCR Testing of Pooled Longitudinally Collected Cervical Specimens of Women to Increase the Efficiency of Studying Human Papillomavirus Infection</atitle><jtitle>Cancer epidemiology, biomarkers & prevention</jtitle><addtitle>Cancer Epidemiol Biomarkers Prev</addtitle><date>2005-01-01</date><risdate>2005</risdate><volume>14</volume><issue>1</issue><spage>256</spage><epage>260</epage><pages>256-260</pages><issn>1055-9965</issn><eissn>1538-7755</eissn><abstract>In large active cohort studies of women investigating human papillomavirus (HPV) and cervical neoplasia, many women will be
HPV-negative at all time points and testing of all their cervical specimens is an inefficient use of laboratory resources.
The aim of this pilot study was to evaluate whether pooling cervical specimens from the same woman might provide a useful
pretest of specimens from women unlikely to have high-grade cervical neoplasia or significant HPV exposure. We selected women
( n = 187) participating in the Guanacaste Project for whom we already had HPV testing data on all their specimens from multiple
visits (median = 8 visits), who were HPV DNA-negative at enrollment and at their 5- to 7-year exit from the cohort, and had
no evidence of high-grade cervical neoplasia. Equal aliquots of cervical specimens from these women were pooled to create
a proportional pooled specimen. Aliquots of pooled specimens were tested in a masked fashion by MY09/11 L1 consensus primer
PCR. Second aliquots of some pooled specimens ( n = 83) were included to assess the reliability of pooled testing. Results were compared with the predicted (expected) results
based on the obtained test results of the individual specimens collected at interim visits. There was good overall agreement
between observed and expected HPV DNA positivity, with a κ of 0.63 [95% confidence interval (95% CI), 0.51-0.75] and a percent agreement of 83.4% (95% CI, 77.3-88.5%) although the
HPV DNA positivity in the pooled specimen was less than expected ( P = 0.001). The agreement between observed and expected HPV DNA positivity was related to the number of aliquots pooled, suggesting
that positivity was related to viral genome concentrations. The κ and percent agreement for intra-batch reliability of testing pooled specimens were 0.68 (95% CI, 0.53-0.84) and 84.3% (95%
CI, 74.7-91.4%), respectively. We conclude that pooling specimens and testing by PCR may be useful for discriminating HPV
DNA-positive from completely negative specimen sets in women who are likely to have been HPV DNA-negative.></abstract><cop>Philadelphia, PA</cop><pub>American Association for Cancer Research</pub><pmid>15668503</pmid><doi>10.1158/1055-9965.256.14.1</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Cancer epidemiology, biomarkers & prevention, 2005-01, Vol.14 (1), p.256-260 |
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| source | MEDLINE; American Association for Cancer Research; EZB-FREE-00999 freely available EZB journals |
| subjects | Biological and medical sciences Cervical Intraepithelial Neoplasia - virology Female Female genital diseases Gynecology. Andrology. Obstetrics Human papillomavirus Humans Longitudinal Studies Medical sciences Papillomaviridae - isolation & purification Papillomavirus Infections - complications Papillomavirus Infections - diagnosis Pilot Projects Polymerase Chain Reaction - methods Reproducibility of Results Tumors Uterine Cervical Neoplasms - virology |
| title | PCR Testing of Pooled Longitudinally Collected Cervical Specimens of Women to Increase the Efficiency of Studying Human Papillomavirus Infection |
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