Structural and Oxidation-State Changes at Its Nonstandard Ni−Fe Site during Activation of the NAD-Reducing Hydrogenase from Ralstonia eutropha Detected by X-ray Absorption, EPR, and FTIR Spectroscopy

Structure and oxidation state of the Ni−Fe cofactor of the NAD-reducing soluble hydrogenase (SH) from Ralstonia eutropha were studied employing X-ray absorption spectroscopy (XAS) at the Ni K-edge, EPR, and FTIR spectroscopy. The SH comprises a nonstandard (CN)Ni−Fe(CN)3(CO) site; its hydrogen-cleav...

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