Characterization of a B220+ Lymphoid Cell Subpopulation with Immune Modulatory Functions in Nasal-Associated Lymphoid Tissues
Complex mechanisms operate on mucosal tissues to regulate immune responsiveness and tolerance. When the lymphocyte subpopulations from murine nasal-associated lymphoid tissues (NALT) were characterized, we observed an accumulation of B220(low)CD3(low)CD4(-)CD8(-)CD19(-)c-Kit(+) cells. TCR transgenic...
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| Veröffentlicht in: | The Journal of immunology (1950) 2005-02, Vol.174 (3), p.1317-1324 |
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| container_title | The Journal of immunology (1950) |
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| creator | Rharbaoui, Faiza Bruder, Dunja Vidakovic, Melita Ebensen, Thomas Buer, Jan Guzman, Carlos A |
| description | Complex mechanisms operate on mucosal tissues to regulate immune responsiveness and tolerance. When the lymphocyte subpopulations from murine nasal-associated lymphoid tissues (NALT) were characterized, we observed an accumulation of B220(low)CD3(low)CD4(-)CD8(-)CD19(-)c-Kit(+) cells. TCR transgenic mice and athymic mice were used for monitoring T cell lineage and the presence of extrathymic T cell precursors. The majority of cells from NALT exhibited a T cell precursor phenotype (CD4(-)CD8(-)CD19(-)c-Kit(+)). Fas-independent apoptosis was their main mechanism of cell death. We also demonstrated that B220(low)CD4(-)CD8(-)CD19(-) cells from NALT exhibited the potential to down-regulate the activation of mature T cells. However, the innate immunity receptor TLR2 was also highly expressed by this cell subpopulation. Moreover, nasal stimulation with a TLR2/6 agonist resulted in a partial activation of the double-negative cells. These results suggest that the immune responses in NALT may be in part modulated by a cell subpopulation that maintains a tolerogenic milieu by its proapoptotic status and suppressive activity, which can be reverted through stimulation of a TLR signaling cascade. |
| doi_str_mv | 10.4049/jimmunol.174.3.1317 |
| format | Article |
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When the lymphocyte subpopulations from murine nasal-associated lymphoid tissues (NALT) were characterized, we observed an accumulation of B220(low)CD3(low)CD4(-)CD8(-)CD19(-)c-Kit(+) cells. TCR transgenic mice and athymic mice were used for monitoring T cell lineage and the presence of extrathymic T cell precursors. The majority of cells from NALT exhibited a T cell precursor phenotype (CD4(-)CD8(-)CD19(-)c-Kit(+)). Fas-independent apoptosis was their main mechanism of cell death. We also demonstrated that B220(low)CD4(-)CD8(-)CD19(-) cells from NALT exhibited the potential to down-regulate the activation of mature T cells. However, the innate immunity receptor TLR2 was also highly expressed by this cell subpopulation. Moreover, nasal stimulation with a TLR2/6 agonist resulted in a partial activation of the double-negative cells. These results suggest that the immune responses in NALT may be in part modulated by a cell subpopulation that maintains a tolerogenic milieu by its proapoptotic status and suppressive activity, which can be reverted through stimulation of a TLR signaling cascade.</description><identifier>ISSN: 0022-1767</identifier><identifier>EISSN: 1550-6606</identifier><identifier>DOI: 10.4049/jimmunol.174.3.1317</identifier><identifier>PMID: 15661888</identifier><language>eng</language><publisher>United States: Am Assoc Immnol</publisher><subject>Animals ; Apoptosis - immunology ; B-Lymphocytes - immunology ; B-Lymphocytes - metabolism ; CD3 Complex - biosynthesis ; CD4 Antigens - metabolism ; CD8 Antigens - metabolism ; Cell Differentiation - immunology ; fas Receptor - physiology ; Female ; Flow Cytometry ; Hematopoietic Stem Cells - immunology ; Hematopoietic Stem Cells - metabolism ; Immunity, Cellular - physiology ; Immunophenotyping ; Leukocyte Common Antigens - biosynthesis ; Lymphocyte Activation - immunology ; Lymphocyte Subsets - immunology ; Lymphocyte Subsets - metabolism ; Lymphoid Tissue - cytology ; Lymphoid Tissue - immunology ; Lymphoid Tissue - metabolism ; Membrane Glycoproteins - physiology ; Mice ; Mice, Inbred BALB C ; Mice, Inbred MRL lpr ; Mice, Nude ; Mice, Transgenic ; Nasal Mucosa - cytology ; Nasal Mucosa - immunology ; Nasal Mucosa - metabolism ; Receptors, Cell Surface - physiology ; Signal Transduction - immunology ; T-Lymphocytes - cytology ; T-Lymphocytes - immunology ; T-Lymphocytes - metabolism ; Toll-Like Receptor 2 ; Toll-Like Receptors</subject><ispartof>The Journal of immunology (1950), 2005-02, Vol.174 (3), p.1317-1324</ispartof><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c380t-d6fed94e39ed6d5f8d8845ed9ad68379bd2b0c40db5aa01311284c25c06a68df3</citedby><cites>FETCH-LOGICAL-c380t-d6fed94e39ed6d5f8d8845ed9ad68379bd2b0c40db5aa01311284c25c06a68df3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,27905,27906</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15661888$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Rharbaoui, Faiza</creatorcontrib><creatorcontrib>Bruder, Dunja</creatorcontrib><creatorcontrib>Vidakovic, Melita</creatorcontrib><creatorcontrib>Ebensen, Thomas</creatorcontrib><creatorcontrib>Buer, Jan</creatorcontrib><creatorcontrib>Guzman, Carlos A</creatorcontrib><title>Characterization of a B220+ Lymphoid Cell Subpopulation with Immune Modulatory Functions in Nasal-Associated Lymphoid Tissues</title><title>The Journal of immunology (1950)</title><addtitle>J Immunol</addtitle><description>Complex mechanisms operate on mucosal tissues to regulate immune responsiveness and tolerance. When the lymphocyte subpopulations from murine nasal-associated lymphoid tissues (NALT) were characterized, we observed an accumulation of B220(low)CD3(low)CD4(-)CD8(-)CD19(-)c-Kit(+) cells. TCR transgenic mice and athymic mice were used for monitoring T cell lineage and the presence of extrathymic T cell precursors. The majority of cells from NALT exhibited a T cell precursor phenotype (CD4(-)CD8(-)CD19(-)c-Kit(+)). Fas-independent apoptosis was their main mechanism of cell death. We also demonstrated that B220(low)CD4(-)CD8(-)CD19(-) cells from NALT exhibited the potential to down-regulate the activation of mature T cells. However, the innate immunity receptor TLR2 was also highly expressed by this cell subpopulation. Moreover, nasal stimulation with a TLR2/6 agonist resulted in a partial activation of the double-negative cells. These results suggest that the immune responses in NALT may be in part modulated by a cell subpopulation that maintains a tolerogenic milieu by its proapoptotic status and suppressive activity, which can be reverted through stimulation of a TLR signaling cascade.</description><subject>Animals</subject><subject>Apoptosis - immunology</subject><subject>B-Lymphocytes - immunology</subject><subject>B-Lymphocytes - metabolism</subject><subject>CD3 Complex - biosynthesis</subject><subject>CD4 Antigens - metabolism</subject><subject>CD8 Antigens - metabolism</subject><subject>Cell Differentiation - immunology</subject><subject>fas Receptor - physiology</subject><subject>Female</subject><subject>Flow Cytometry</subject><subject>Hematopoietic Stem Cells - immunology</subject><subject>Hematopoietic Stem Cells - metabolism</subject><subject>Immunity, Cellular - physiology</subject><subject>Immunophenotyping</subject><subject>Leukocyte Common Antigens - biosynthesis</subject><subject>Lymphocyte Activation - immunology</subject><subject>Lymphocyte Subsets - immunology</subject><subject>Lymphocyte Subsets - metabolism</subject><subject>Lymphoid Tissue - cytology</subject><subject>Lymphoid Tissue - immunology</subject><subject>Lymphoid Tissue - metabolism</subject><subject>Membrane Glycoproteins - physiology</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred MRL lpr</subject><subject>Mice, Nude</subject><subject>Mice, Transgenic</subject><subject>Nasal Mucosa - cytology</subject><subject>Nasal Mucosa - immunology</subject><subject>Nasal Mucosa - metabolism</subject><subject>Receptors, Cell Surface - physiology</subject><subject>Signal Transduction - immunology</subject><subject>T-Lymphocytes - cytology</subject><subject>T-Lymphocytes - immunology</subject><subject>T-Lymphocytes - metabolism</subject><subject>Toll-Like Receptor 2</subject><subject>Toll-Like Receptors</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1v1DAQhi0EotvCL0BCPsEBZRl_xPEe2xUtlRY4UM6WYzvEVRIHO1G0SPx3HO2icrI0fuadmQehNwS2HPju46Pv-3kI3ZZUfMu2hJHqGdqQsoRCCBDP0QaA0oJUorpAlyk9AoAAyl-iC1IKQaSUG_Rn3-qozeSi_60nHwYcGqzxDaXwAR-O_dgGb_HedR3-PtdjGOfuhC1-avH9uoHDX4JdyyEe8e08mPU_YT_grzrprrhOKRivJ2efAh98SrNLr9CLRnfJvT6_V-jH7aeH_efi8O3ufn99KAyTMBVWNM7uuGM7Z4UtG2ml5GUuaSskq3a1pTUYDrYutYYsglDJDS0NCC2kbdgVenfKHWP4ledOqvfJ5KP04MKclKiYqDjQDLITaGJIKbpGjdH3Oh4VAbVaV_-sq2xdMbVaz11vz_Fz3Tv71HPWnIH3J6D1P9vFR6dSr7su40Qty_Jf1F9epo_1</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Rharbaoui, Faiza</creator><creator>Bruder, Dunja</creator><creator>Vidakovic, Melita</creator><creator>Ebensen, Thomas</creator><creator>Buer, Jan</creator><creator>Guzman, Carlos A</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Characterization of a B220+ Lymphoid Cell Subpopulation with Immune Modulatory Functions in Nasal-Associated Lymphoid Tissues</title><author>Rharbaoui, Faiza ; Bruder, Dunja ; Vidakovic, Melita ; Ebensen, Thomas ; Buer, Jan ; Guzman, Carlos A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c380t-d6fed94e39ed6d5f8d8845ed9ad68379bd2b0c40db5aa01311284c25c06a68df3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Apoptosis - immunology</topic><topic>B-Lymphocytes - immunology</topic><topic>B-Lymphocytes - metabolism</topic><topic>CD3 Complex - biosynthesis</topic><topic>CD4 Antigens - metabolism</topic><topic>CD8 Antigens - metabolism</topic><topic>Cell Differentiation - immunology</topic><topic>fas Receptor - physiology</topic><topic>Female</topic><topic>Flow Cytometry</topic><topic>Hematopoietic Stem Cells - immunology</topic><topic>Hematopoietic Stem Cells - metabolism</topic><topic>Immunity, Cellular - physiology</topic><topic>Immunophenotyping</topic><topic>Leukocyte Common Antigens - biosynthesis</topic><topic>Lymphocyte Activation - immunology</topic><topic>Lymphocyte Subsets - immunology</topic><topic>Lymphocyte Subsets - metabolism</topic><topic>Lymphoid Tissue - cytology</topic><topic>Lymphoid Tissue - immunology</topic><topic>Lymphoid Tissue - metabolism</topic><topic>Membrane Glycoproteins - physiology</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred MRL lpr</topic><topic>Mice, Nude</topic><topic>Mice, Transgenic</topic><topic>Nasal Mucosa - cytology</topic><topic>Nasal Mucosa - immunology</topic><topic>Nasal Mucosa - metabolism</topic><topic>Receptors, Cell Surface - physiology</topic><topic>Signal Transduction - immunology</topic><topic>T-Lymphocytes - cytology</topic><topic>T-Lymphocytes - immunology</topic><topic>T-Lymphocytes - metabolism</topic><topic>Toll-Like Receptor 2</topic><topic>Toll-Like Receptors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rharbaoui, Faiza</creatorcontrib><creatorcontrib>Bruder, Dunja</creatorcontrib><creatorcontrib>Vidakovic, Melita</creatorcontrib><creatorcontrib>Ebensen, Thomas</creatorcontrib><creatorcontrib>Buer, Jan</creatorcontrib><creatorcontrib>Guzman, Carlos A</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rharbaoui, Faiza</au><au>Bruder, Dunja</au><au>Vidakovic, Melita</au><au>Ebensen, Thomas</au><au>Buer, Jan</au><au>Guzman, Carlos A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Characterization of a B220+ Lymphoid Cell Subpopulation with Immune Modulatory Functions in Nasal-Associated Lymphoid Tissues</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>174</volume><issue>3</issue><spage>1317</spage><epage>1324</epage><pages>1317-1324</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>Complex mechanisms operate on mucosal tissues to regulate immune responsiveness and tolerance. When the lymphocyte subpopulations from murine nasal-associated lymphoid tissues (NALT) were characterized, we observed an accumulation of B220(low)CD3(low)CD4(-)CD8(-)CD19(-)c-Kit(+) cells. TCR transgenic mice and athymic mice were used for monitoring T cell lineage and the presence of extrathymic T cell precursors. The majority of cells from NALT exhibited a T cell precursor phenotype (CD4(-)CD8(-)CD19(-)c-Kit(+)). Fas-independent apoptosis was their main mechanism of cell death. We also demonstrated that B220(low)CD4(-)CD8(-)CD19(-) cells from NALT exhibited the potential to down-regulate the activation of mature T cells. However, the innate immunity receptor TLR2 was also highly expressed by this cell subpopulation. Moreover, nasal stimulation with a TLR2/6 agonist resulted in a partial activation of the double-negative cells. 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| issn | 0022-1767 1550-6606 |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Alma/SFX Local Collection |
| subjects | Animals Apoptosis - immunology B-Lymphocytes - immunology B-Lymphocytes - metabolism CD3 Complex - biosynthesis CD4 Antigens - metabolism CD8 Antigens - metabolism Cell Differentiation - immunology fas Receptor - physiology Female Flow Cytometry Hematopoietic Stem Cells - immunology Hematopoietic Stem Cells - metabolism Immunity, Cellular - physiology Immunophenotyping Leukocyte Common Antigens - biosynthesis Lymphocyte Activation - immunology Lymphocyte Subsets - immunology Lymphocyte Subsets - metabolism Lymphoid Tissue - cytology Lymphoid Tissue - immunology Lymphoid Tissue - metabolism Membrane Glycoproteins - physiology Mice Mice, Inbred BALB C Mice, Inbred MRL lpr Mice, Nude Mice, Transgenic Nasal Mucosa - cytology Nasal Mucosa - immunology Nasal Mucosa - metabolism Receptors, Cell Surface - physiology Signal Transduction - immunology T-Lymphocytes - cytology T-Lymphocytes - immunology T-Lymphocytes - metabolism Toll-Like Receptor 2 Toll-Like Receptors |
| title | Characterization of a B220+ Lymphoid Cell Subpopulation with Immune Modulatory Functions in Nasal-Associated Lymphoid Tissues |
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