Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway
Summary Erwinia carotovora produces the β‐lactam antibiotic, carbapenem, in response to a quorum sensing signalling molecule, N‐(3‐oxohexanoyl)‐ l‐homoserine lactone (OHHL). We have mapped the OHHL‐dependent promoter upstream of the first of the biosynthetic genes, carA. We have also analysed the ef...
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| Veröffentlicht in: | Molecular microbiology 2005-01, Vol.55 (2), p.526-545 |
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| creator | McGowan, Simon J. Barnard, Anne M. L. Bosgelmez, Gulgun Sebaihia, Mohammed Simpson, Natalie J. L. Thomson, Nicholas R. Todd, Daniel E. Welch, Martin Whitehead, Neil A. Salmond, George P. C. |
| description | Summary
Erwinia carotovora produces the β‐lactam antibiotic, carbapenem, in response to a quorum sensing signalling molecule, N‐(3‐oxohexanoyl)‐ l‐homoserine lactone (OHHL). We have mapped the OHHL‐dependent promoter upstream of the first of the biosynthetic genes, carA. We have also analysed the effect on this promoter of the known genetic regulators of carbapenem expression, carR, carI (encoding homologues of LuxR and LuxI respectively) and hor (encoding a SlyA/MarR‐like transcriptional regulator). We describe a previously unknown promoter located within the carA‐H operon. This promoter does not respond to CarR and is required for quorum sensing‐independent expression of the carbapenem resistance determinants encoded by the carFG genes. We have mapped the carR, carI and hor transcription start points, shown that CarR is positively autoregulated in the presence of OHHL, and have demonstrated negative feedback affecting transcription of carI. In addition, various environmental and physiological factors were shown to impinge on the transcription of the car biosynthetic genes. The nature of the carbon source and the temperature of growth influence carbapenem production by modulating the level of the OHHL signalling molecule, and thereby physiologically fine‐tune the quorum sensing regulatory system. |
| doi_str_mv | 10.1111/j.1365-2958.2004.04397.x |
| format | Article |
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Erwinia carotovora produces the β‐lactam antibiotic, carbapenem, in response to a quorum sensing signalling molecule, N‐(3‐oxohexanoyl)‐ l‐homoserine lactone (OHHL). We have mapped the OHHL‐dependent promoter upstream of the first of the biosynthetic genes, carA. We have also analysed the effect on this promoter of the known genetic regulators of carbapenem expression, carR, carI (encoding homologues of LuxR and LuxI respectively) and hor (encoding a SlyA/MarR‐like transcriptional regulator). We describe a previously unknown promoter located within the carA‐H operon. This promoter does not respond to CarR and is required for quorum sensing‐independent expression of the carbapenem resistance determinants encoded by the carFG genes. We have mapped the carR, carI and hor transcription start points, shown that CarR is positively autoregulated in the presence of OHHL, and have demonstrated negative feedback affecting transcription of carI. In addition, various environmental and physiological factors were shown to impinge on the transcription of the car biosynthetic genes. The nature of the carbon source and the temperature of growth influence carbapenem production by modulating the level of the OHHL signalling molecule, and thereby physiologically fine‐tune the quorum sensing regulatory system.</description><identifier>ISSN: 0950-382X</identifier><identifier>EISSN: 1365-2958</identifier><identifier>DOI: 10.1111/j.1365-2958.2004.04397.x</identifier><identifier>PMID: 15659168</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Science Ltd</publisher><subject>4-Butyrolactone - analogs & derivatives ; 4-Butyrolactone - metabolism ; Antibiotics ; Bacterial Proteins - genetics ; Bacterial Proteins - metabolism ; Bacteriology ; Biological and medical sciences ; Carbapenems - biosynthesis ; Carbon - metabolism ; Culture Media ; Erwinia carotovora ; Fundamental and applied biological sciences. Psychology ; Gene expression ; Gene Expression Regulation, Bacterial ; Genetics ; Microbiology ; Miscellaneous ; Molecular biology ; Operon ; Oxygen - pharmacology ; Pectobacterium carotovorum - genetics ; Pectobacterium carotovorum - growth & development ; Pectobacterium carotovorum - metabolism ; Pectobacterium carotovorum - physiology ; Promoter Regions, Genetic ; Signal Transduction ; Temperature ; Transcription, Genetic</subject><ispartof>Molecular microbiology, 2005-01, Vol.55 (2), p.526-545</ispartof><rights>2005 INIST-CNRS</rights><rights>Copyright Blackwell Publishing Jan 2005</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5717-2d58ec9b802dc426abdf6b0cbba738d172df3ff101719390ede7cf488450476f3</citedby><cites>FETCH-LOGICAL-c5717-2d58ec9b802dc426abdf6b0cbba738d172df3ff101719390ede7cf488450476f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1365-2958.2004.04397.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1365-2958.2004.04397.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,1433,27924,27925,45574,45575,46409,46833</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16535317$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15659168$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>McGowan, Simon J.</creatorcontrib><creatorcontrib>Barnard, Anne M. L.</creatorcontrib><creatorcontrib>Bosgelmez, Gulgun</creatorcontrib><creatorcontrib>Sebaihia, Mohammed</creatorcontrib><creatorcontrib>Simpson, Natalie J. L.</creatorcontrib><creatorcontrib>Thomson, Nicholas R.</creatorcontrib><creatorcontrib>Todd, Daniel E.</creatorcontrib><creatorcontrib>Welch, Martin</creatorcontrib><creatorcontrib>Whitehead, Neil A.</creatorcontrib><creatorcontrib>Salmond, George P. C.</creatorcontrib><title>Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway</title><title>Molecular microbiology</title><addtitle>Mol Microbiol</addtitle><description>Summary
Erwinia carotovora produces the β‐lactam antibiotic, carbapenem, in response to a quorum sensing signalling molecule, N‐(3‐oxohexanoyl)‐ l‐homoserine lactone (OHHL). We have mapped the OHHL‐dependent promoter upstream of the first of the biosynthetic genes, carA. We have also analysed the effect on this promoter of the known genetic regulators of carbapenem expression, carR, carI (encoding homologues of LuxR and LuxI respectively) and hor (encoding a SlyA/MarR‐like transcriptional regulator). We describe a previously unknown promoter located within the carA‐H operon. This promoter does not respond to CarR and is required for quorum sensing‐independent expression of the carbapenem resistance determinants encoded by the carFG genes. We have mapped the carR, carI and hor transcription start points, shown that CarR is positively autoregulated in the presence of OHHL, and have demonstrated negative feedback affecting transcription of carI. In addition, various environmental and physiological factors were shown to impinge on the transcription of the car biosynthetic genes. The nature of the carbon source and the temperature of growth influence carbapenem production by modulating the level of the OHHL signalling molecule, and thereby physiologically fine‐tune the quorum sensing regulatory system.</description><subject>4-Butyrolactone - analogs & derivatives</subject><subject>4-Butyrolactone - metabolism</subject><subject>Antibiotics</subject><subject>Bacterial Proteins - genetics</subject><subject>Bacterial Proteins - metabolism</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carbapenems - biosynthesis</subject><subject>Carbon - metabolism</subject><subject>Culture Media</subject><subject>Erwinia carotovora</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Bacterial</subject><subject>Genetics</subject><subject>Microbiology</subject><subject>Miscellaneous</subject><subject>Molecular biology</subject><subject>Operon</subject><subject>Oxygen - pharmacology</subject><subject>Pectobacterium carotovorum - genetics</subject><subject>Pectobacterium carotovorum - growth & development</subject><subject>Pectobacterium carotovorum - metabolism</subject><subject>Pectobacterium carotovorum - physiology</subject><subject>Promoter Regions, Genetic</subject><subject>Signal Transduction</subject><subject>Temperature</subject><subject>Transcription, Genetic</subject><issn>0950-382X</issn><issn>1365-2958</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkc1u1DAUhSMEokPhFZCFBLsEO47tZMECjQpUasUGJHaW458ZjxJ7ajtMs-MR-hB9Mp4EhxlRiQ14c617v3t0rk5RAAQrlN_bXYUwJWXdkbaqIWwq2OCOVbePitWfweNiBTsCS9zW386KZzHuIEQYUvy0OEOEkg7RdlXcr0XoxV47PQLhku2tT1aCXOLs0lZHG4F14CIcrLMCSBF88t99ECAPgt5Mg0hagX4G--0crR_8xkoxZC0FNll1ETNCJh8iGL1acOs2ICuDm8mHaQRRu5hbP3_cKZ19KO0SkN6l4AewF2l7EPPz4okRQ9QvTvW8-Prh4sv6U3n1-ePl-v1VKQlDrKwVabXs-hbWSjY1Fb0ytIey7wXDrUKsVgYbgyBiqMMd1EozaZq2bQhsGDX4vHhz1N0HfzPpmPhoo9TDIJz2U-SUYcoy-k8QMYJrSLsMvvoL3PkpuHwERx0lGFFSZ6g9QjL4GIM2fB_sKMLMEeRL3nzHl1j5Eitf8ua_8-a3efXlSX_qR60eFk8BZ-D1CRAx52KCcNLGBy57yC6Wi94duYMd9PzfBvj19eXyw78AtbfL6w</recordid><startdate>200501</startdate><enddate>200501</enddate><creator>McGowan, Simon J.</creator><creator>Barnard, Anne M. L.</creator><creator>Bosgelmez, Gulgun</creator><creator>Sebaihia, Mohammed</creator><creator>Simpson, Natalie J. L.</creator><creator>Thomson, Nicholas R.</creator><creator>Todd, Daniel E.</creator><creator>Welch, Martin</creator><creator>Whitehead, Neil A.</creator><creator>Salmond, George P. C.</creator><general>Blackwell Science Ltd</general><general>Blackwell Science</general><general>Blackwell Publishing Ltd</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7TK</scope><scope>7TM</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>200501</creationdate><title>Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway</title><author>McGowan, Simon J. ; Barnard, Anne M. L. ; Bosgelmez, Gulgun ; Sebaihia, Mohammed ; Simpson, Natalie J. L. ; Thomson, Nicholas R. ; Todd, Daniel E. ; Welch, Martin ; Whitehead, Neil A. ; Salmond, George P. C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5717-2d58ec9b802dc426abdf6b0cbba738d172df3ff101719390ede7cf488450476f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>4-Butyrolactone - analogs & derivatives</topic><topic>4-Butyrolactone - metabolism</topic><topic>Antibiotics</topic><topic>Bacterial Proteins - genetics</topic><topic>Bacterial Proteins - metabolism</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carbapenems - biosynthesis</topic><topic>Carbon - metabolism</topic><topic>Culture Media</topic><topic>Erwinia carotovora</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene expression</topic><topic>Gene Expression Regulation, Bacterial</topic><topic>Genetics</topic><topic>Microbiology</topic><topic>Miscellaneous</topic><topic>Molecular biology</topic><topic>Operon</topic><topic>Oxygen - pharmacology</topic><topic>Pectobacterium carotovorum - genetics</topic><topic>Pectobacterium carotovorum - growth & development</topic><topic>Pectobacterium carotovorum - metabolism</topic><topic>Pectobacterium carotovorum - physiology</topic><topic>Promoter Regions, Genetic</topic><topic>Signal Transduction</topic><topic>Temperature</topic><topic>Transcription, Genetic</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>McGowan, Simon J.</creatorcontrib><creatorcontrib>Barnard, Anne M. L.</creatorcontrib><creatorcontrib>Bosgelmez, Gulgun</creatorcontrib><creatorcontrib>Sebaihia, Mohammed</creatorcontrib><creatorcontrib>Simpson, Natalie J. L.</creatorcontrib><creatorcontrib>Thomson, Nicholas R.</creatorcontrib><creatorcontrib>Todd, Daniel E.</creatorcontrib><creatorcontrib>Welch, Martin</creatorcontrib><creatorcontrib>Whitehead, Neil A.</creatorcontrib><creatorcontrib>Salmond, George P. C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Molecular microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>McGowan, Simon J.</au><au>Barnard, Anne M. L.</au><au>Bosgelmez, Gulgun</au><au>Sebaihia, Mohammed</au><au>Simpson, Natalie J. L.</au><au>Thomson, Nicholas R.</au><au>Todd, Daniel E.</au><au>Welch, Martin</au><au>Whitehead, Neil A.</au><au>Salmond, George P. C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway</atitle><jtitle>Molecular microbiology</jtitle><addtitle>Mol Microbiol</addtitle><date>2005-01</date><risdate>2005</risdate><volume>55</volume><issue>2</issue><spage>526</spage><epage>545</epage><pages>526-545</pages><issn>0950-382X</issn><eissn>1365-2958</eissn><abstract>Summary
Erwinia carotovora produces the β‐lactam antibiotic, carbapenem, in response to a quorum sensing signalling molecule, N‐(3‐oxohexanoyl)‐ l‐homoserine lactone (OHHL). We have mapped the OHHL‐dependent promoter upstream of the first of the biosynthetic genes, carA. We have also analysed the effect on this promoter of the known genetic regulators of carbapenem expression, carR, carI (encoding homologues of LuxR and LuxI respectively) and hor (encoding a SlyA/MarR‐like transcriptional regulator). We describe a previously unknown promoter located within the carA‐H operon. This promoter does not respond to CarR and is required for quorum sensing‐independent expression of the carbapenem resistance determinants encoded by the carFG genes. We have mapped the carR, carI and hor transcription start points, shown that CarR is positively autoregulated in the presence of OHHL, and have demonstrated negative feedback affecting transcription of carI. In addition, various environmental and physiological factors were shown to impinge on the transcription of the car biosynthetic genes. The nature of the carbon source and the temperature of growth influence carbapenem production by modulating the level of the OHHL signalling molecule, and thereby physiologically fine‐tune the quorum sensing regulatory system.</abstract><cop>Oxford, UK</cop><pub>Blackwell Science Ltd</pub><pmid>15659168</pmid><doi>10.1111/j.1365-2958.2004.04397.x</doi><tpages>20</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | 4-Butyrolactone - analogs & derivatives 4-Butyrolactone - metabolism Antibiotics Bacterial Proteins - genetics Bacterial Proteins - metabolism Bacteriology Biological and medical sciences Carbapenems - biosynthesis Carbon - metabolism Culture Media Erwinia carotovora Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Regulation, Bacterial Genetics Microbiology Miscellaneous Molecular biology Operon Oxygen - pharmacology Pectobacterium carotovorum - genetics Pectobacterium carotovorum - growth & development Pectobacterium carotovorum - metabolism Pectobacterium carotovorum - physiology Promoter Regions, Genetic Signal Transduction Temperature Transcription, Genetic |
| title | Carbapenem antibiotic biosynthesis in Erwinia carotovora is regulated by physiological and genetic factors modulating the quorum sensing‐dependent control pathway |
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