Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells
The antioxidant status of cancer cells is an important factor in tumor invasion and metastases. This study investigated whether metastatic cancer cells derive beneficial antioxidant protection from ascitic fluid and are rendered resistant to oxidative stress in the form of a chemically generated fre...
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| Veröffentlicht in: | Gynecologic oncology 2005-02, Vol.96 (2), p.430-438 |
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| creator | Yang, Wenxuan Toffa, Samuel E. Lohn, Jonathan W.G. Seifalian, Alexander M. Winslet, Marc C. |
| description | The antioxidant status of cancer cells is an important factor in tumor invasion and metastases. This study investigated whether metastatic cancer cells derive beneficial antioxidant protection from ascitic fluid and are rendered resistant to oxidative stress in the form of a chemically generated free radical insult.
Human gastric carcinoma (KATO-III) and ovarian adenocarcinoma (SKOV-3) cell lines were cultured and incubated for 24 h with (1) M199 medium; (2) M199 + 20% fetal calf serum (FCS); (3) malignant ascites. All cells were exposed to a hydroxyl radical-generating system for 1 h. Cellular lipid peroxidation was assessed by measuring malondialdehyde (MDA) in cell suspensions. Glutathione (GSH) levels in cell pellet were measured in SKOV-3 cells after 0, 24, 48, and 72 h of incubation with buthionine sulphoximine (BSO). CD44 gene expression of cancer cells was analyzed by Northern blotting.
The results showed that the cancer cells were rendered resistant to oxidative stress and with upregulated CD44 gene expression by components of malignant ascites.
These findings suggest that malignant ascites increases the antioxidant ability of cancer cells and the potential of adhesion and invasion. Thus, determination of the nature of these putative tumor-protective components of ascites may provide targets for therapeutic intervention. |
| doi_str_mv | 10.1016/j.ygyno.2004.10.016 |
| format | Article |
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Human gastric carcinoma (KATO-III) and ovarian adenocarcinoma (SKOV-3) cell lines were cultured and incubated for 24 h with (1) M199 medium; (2) M199 + 20% fetal calf serum (FCS); (3) malignant ascites. All cells were exposed to a hydroxyl radical-generating system for 1 h. Cellular lipid peroxidation was assessed by measuring malondialdehyde (MDA) in cell suspensions. Glutathione (GSH) levels in cell pellet were measured in SKOV-3 cells after 0, 24, 48, and 72 h of incubation with buthionine sulphoximine (BSO). CD44 gene expression of cancer cells was analyzed by Northern blotting.
The results showed that the cancer cells were rendered resistant to oxidative stress and with upregulated CD44 gene expression by components of malignant ascites.
These findings suggest that malignant ascites increases the antioxidant ability of cancer cells and the potential of adhesion and invasion. Thus, determination of the nature of these putative tumor-protective components of ascites may provide targets for therapeutic intervention.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1016/j.ygyno.2004.10.016</identifier><identifier>PMID: 15661232</identifier><language>eng</language><publisher>United States: Elsevier Inc</publisher><subject>Adenocarcinoma - genetics ; Adenocarcinoma - metabolism ; Adenocarcinoma - pathology ; Antioxidant ; Antioxidants - metabolism ; Ascites - metabolism ; Ascites - pathology ; Blotting, Northern ; Buthionine Sulfoximine - pharmacology ; CD44 ; Cell Adhesion - physiology ; Cell Line, Tumor ; Cell Survival - drug effects ; Female ; Gastric cancer ; Gene Expression ; Glutathione ; Glutathione - deficiency ; Glutathione - metabolism ; Humans ; Hyaluronan Receptors - biosynthesis ; Hyaluronan Receptors - genetics ; Lipid Peroxidation ; Malignant ascites ; Malondialdehyde - metabolism ; Metastases ; Ovarian cancer ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - metabolism ; Ovarian Neoplasms - pathology ; Oxidative Stress ; Stomach Neoplasms - genetics ; Stomach Neoplasms - metabolism ; Stomach Neoplasms - pathology</subject><ispartof>Gynecologic oncology, 2005-02, Vol.96 (2), p.430-438</ispartof><rights>2004 Elsevier Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c357t-af9da840f3d01e55bea8a2d52d8fbfe5eb3f033addfb6d356ba1b5c446910eee3</citedby><cites>FETCH-LOGICAL-c357t-af9da840f3d01e55bea8a2d52d8fbfe5eb3f033addfb6d356ba1b5c446910eee3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.ygyno.2004.10.016$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,776,780,3536,27903,27904,45974</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15661232$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yang, Wenxuan</creatorcontrib><creatorcontrib>Toffa, Samuel E.</creatorcontrib><creatorcontrib>Lohn, Jonathan W.G.</creatorcontrib><creatorcontrib>Seifalian, Alexander M.</creatorcontrib><creatorcontrib>Winslet, Marc C.</creatorcontrib><title>Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>The antioxidant status of cancer cells is an important factor in tumor invasion and metastases. This study investigated whether metastatic cancer cells derive beneficial antioxidant protection from ascitic fluid and are rendered resistant to oxidative stress in the form of a chemically generated free radical insult.
Human gastric carcinoma (KATO-III) and ovarian adenocarcinoma (SKOV-3) cell lines were cultured and incubated for 24 h with (1) M199 medium; (2) M199 + 20% fetal calf serum (FCS); (3) malignant ascites. All cells were exposed to a hydroxyl radical-generating system for 1 h. Cellular lipid peroxidation was assessed by measuring malondialdehyde (MDA) in cell suspensions. Glutathione (GSH) levels in cell pellet were measured in SKOV-3 cells after 0, 24, 48, and 72 h of incubation with buthionine sulphoximine (BSO). CD44 gene expression of cancer cells was analyzed by Northern blotting.
The results showed that the cancer cells were rendered resistant to oxidative stress and with upregulated CD44 gene expression by components of malignant ascites.
These findings suggest that malignant ascites increases the antioxidant ability of cancer cells and the potential of adhesion and invasion. Thus, determination of the nature of these putative tumor-protective components of ascites may provide targets for therapeutic intervention.</description><subject>Adenocarcinoma - genetics</subject><subject>Adenocarcinoma - metabolism</subject><subject>Adenocarcinoma - pathology</subject><subject>Antioxidant</subject><subject>Antioxidants - metabolism</subject><subject>Ascites - metabolism</subject><subject>Ascites - pathology</subject><subject>Blotting, Northern</subject><subject>Buthionine Sulfoximine - pharmacology</subject><subject>CD44</subject><subject>Cell Adhesion - physiology</subject><subject>Cell Line, Tumor</subject><subject>Cell Survival - drug effects</subject><subject>Female</subject><subject>Gastric cancer</subject><subject>Gene Expression</subject><subject>Glutathione</subject><subject>Glutathione - deficiency</subject><subject>Glutathione - metabolism</subject><subject>Humans</subject><subject>Hyaluronan Receptors - biosynthesis</subject><subject>Hyaluronan Receptors - genetics</subject><subject>Lipid Peroxidation</subject><subject>Malignant ascites</subject><subject>Malondialdehyde - metabolism</subject><subject>Metastases</subject><subject>Ovarian cancer</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - metabolism</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Oxidative Stress</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - metabolism</subject><subject>Stomach Neoplasms - pathology</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFuGyEQhlHVqnHTPEGlilOVHNaBxeDdQw9RlLZWUvmQNFc0C4ODtQsprKPu2xfHlnrradDPNzPwEfKJszlnXF1u59NmCnFeM7Yoybxkb8iMs1ZWqpHtWzJjrGVVU8vmhHzIecsYE4zX78kJl0rxWtQzMv2E3m8ChJFCNn7ETH0wCSGX0_iEtNz4-MfbV6LzvR8nGh192g0QaHyB5Es9v79dP1biotCWbiCPyRsKFkM0kIwPcQB6fnv1sK5Wq9UFNdj3-SN556DPeHasp-TXt5uH6x_V3fr76vrqrjJCLscKXGuhWTAnLOMoZYfQQG1lbRvXOZTYCceEAGtdp6yQqgPeSbNYqJYzRBSn5Mth7nOKv3eYRz34vH8BBIy7rNVSKLnkTQHFATQp5pzQ6efkB0iT5kzvjeutfjWu98b3YclK1-fj-F03oP3Xc1RcgK8HAMsnXzwmXTxjMGh9QjNqG_1_F_wF3y-Uug</recordid><startdate>20050201</startdate><enddate>20050201</enddate><creator>Yang, Wenxuan</creator><creator>Toffa, Samuel E.</creator><creator>Lohn, Jonathan W.G.</creator><creator>Seifalian, Alexander M.</creator><creator>Winslet, Marc C.</creator><general>Elsevier Inc</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20050201</creationdate><title>Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells</title><author>Yang, Wenxuan ; Toffa, Samuel E. ; Lohn, Jonathan W.G. ; Seifalian, Alexander M. ; Winslet, Marc C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c357t-af9da840f3d01e55bea8a2d52d8fbfe5eb3f033addfb6d356ba1b5c446910eee3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Adenocarcinoma - genetics</topic><topic>Adenocarcinoma - metabolism</topic><topic>Adenocarcinoma - pathology</topic><topic>Antioxidant</topic><topic>Antioxidants - metabolism</topic><topic>Ascites - metabolism</topic><topic>Ascites - pathology</topic><topic>Blotting, Northern</topic><topic>Buthionine Sulfoximine - pharmacology</topic><topic>CD44</topic><topic>Cell Adhesion - physiology</topic><topic>Cell Line, Tumor</topic><topic>Cell Survival - drug effects</topic><topic>Female</topic><topic>Gastric cancer</topic><topic>Gene Expression</topic><topic>Glutathione</topic><topic>Glutathione - deficiency</topic><topic>Glutathione - metabolism</topic><topic>Humans</topic><topic>Hyaluronan Receptors - biosynthesis</topic><topic>Hyaluronan Receptors - genetics</topic><topic>Lipid Peroxidation</topic><topic>Malignant ascites</topic><topic>Malondialdehyde - metabolism</topic><topic>Metastases</topic><topic>Ovarian cancer</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - metabolism</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Oxidative Stress</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - metabolism</topic><topic>Stomach Neoplasms - pathology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Yang, Wenxuan</creatorcontrib><creatorcontrib>Toffa, Samuel E.</creatorcontrib><creatorcontrib>Lohn, Jonathan W.G.</creatorcontrib><creatorcontrib>Seifalian, Alexander M.</creatorcontrib><creatorcontrib>Winslet, Marc C.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Yang, Wenxuan</au><au>Toffa, Samuel E.</au><au>Lohn, Jonathan W.G.</au><au>Seifalian, Alexander M.</au><au>Winslet, Marc C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>2005-02-01</date><risdate>2005</risdate><volume>96</volume><issue>2</issue><spage>430</spage><epage>438</epage><pages>430-438</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><abstract>The antioxidant status of cancer cells is an important factor in tumor invasion and metastases. This study investigated whether metastatic cancer cells derive beneficial antioxidant protection from ascitic fluid and are rendered resistant to oxidative stress in the form of a chemically generated free radical insult.
Human gastric carcinoma (KATO-III) and ovarian adenocarcinoma (SKOV-3) cell lines were cultured and incubated for 24 h with (1) M199 medium; (2) M199 + 20% fetal calf serum (FCS); (3) malignant ascites. All cells were exposed to a hydroxyl radical-generating system for 1 h. Cellular lipid peroxidation was assessed by measuring malondialdehyde (MDA) in cell suspensions. Glutathione (GSH) levels in cell pellet were measured in SKOV-3 cells after 0, 24, 48, and 72 h of incubation with buthionine sulphoximine (BSO). CD44 gene expression of cancer cells was analyzed by Northern blotting.
The results showed that the cancer cells were rendered resistant to oxidative stress and with upregulated CD44 gene expression by components of malignant ascites.
These findings suggest that malignant ascites increases the antioxidant ability of cancer cells and the potential of adhesion and invasion. Thus, determination of the nature of these putative tumor-protective components of ascites may provide targets for therapeutic intervention.</abstract><cop>United States</cop><pub>Elsevier Inc</pub><pmid>15661232</pmid><doi>10.1016/j.ygyno.2004.10.016</doi><tpages>9</tpages></addata></record> |
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| subjects | Adenocarcinoma - genetics Adenocarcinoma - metabolism Adenocarcinoma - pathology Antioxidant Antioxidants - metabolism Ascites - metabolism Ascites - pathology Blotting, Northern Buthionine Sulfoximine - pharmacology CD44 Cell Adhesion - physiology Cell Line, Tumor Cell Survival - drug effects Female Gastric cancer Gene Expression Glutathione Glutathione - deficiency Glutathione - metabolism Humans Hyaluronan Receptors - biosynthesis Hyaluronan Receptors - genetics Lipid Peroxidation Malignant ascites Malondialdehyde - metabolism Metastases Ovarian cancer Ovarian Neoplasms - genetics Ovarian Neoplasms - metabolism Ovarian Neoplasms - pathology Oxidative Stress Stomach Neoplasms - genetics Stomach Neoplasms - metabolism Stomach Neoplasms - pathology |
| title | Malignant ascites increases the antioxidant ability of human ovarian (SKOV-3) and gastric adenocarcinoma (KATO-III) cells |
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