Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites
Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of seconda...
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| Veröffentlicht in: | Plant cell reports 2005, Vol.23 (8), p.557-566 |
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| creator | Choi, D.W Jung, J.D Ha, Y.I Park, H.W In, D.S Chung, H.J Liu, J.R |
| description | Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment. |
| doi_str_mv | 10.1007/s00299-004-0845-4 |
| format | Article |
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Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment.</description><identifier>ISSN: 0721-7714</identifier><identifier>EISSN: 1432-203X</identifier><identifier>DOI: 10.1007/s00299-004-0845-4</identifier><identifier>PMID: 15538577</identifier><identifier>CODEN: PCRPD8</identifier><language>eng</language><publisher>Berlin: Springer</publisher><subject>Acetates - pharmacology ; Base Sequence ; Biological and medical sciences ; Biosynthesis ; Biotechnology ; complementary DNA ; Cyclopentanes - pharmacology ; DNA libraries ; DNA, Plant - genetics ; Eukaryotic cell cultures ; expressed sequence tags ; Fundamental and applied biological sciences. Psychology ; gene expression regulation ; Genes, Plant - drug effects ; Genetic resources ; ginsenoside ; Ginsenosides - biosynthesis ; Metabolism ; Metabolites ; Methods. Procedures. Technologies ; methyl jasmonate ; Miscellaneous ; Oxylipins ; Panax - drug effects ; Panax - genetics ; Panax ginseng ; Panax pseudoginseng ; phytochemicals ; Plant cells and fungal cells ; plant proteins ; Plant Proteins - genetics ; Plant Roots - drug effects ; Plant Roots - genetics ; RNA, Plant - drug effects ; RNA, Plant - genetics ; root hairs ; Roots ; Secondary metabolites ; sequence analysis ; Transcription, Genetic ; triterpenoids</subject><ispartof>Plant cell reports, 2005, Vol.23 (8), p.557-566</ispartof><rights>2005 INIST-CNRS</rights><rights>Springer-Verlag 2005</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c411t-41d3f20b0b28f61905ba7fc14f0a1ace287a5bb76931a23b22bbca8c9ea05d7c3</citedby><cites>FETCH-LOGICAL-c411t-41d3f20b0b28f61905ba7fc14f0a1ace287a5bb76931a23b22bbca8c9ea05d7c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16402835$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15538577$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Choi, D.W</creatorcontrib><creatorcontrib>Jung, J.D</creatorcontrib><creatorcontrib>Ha, Y.I</creatorcontrib><creatorcontrib>Park, H.W</creatorcontrib><creatorcontrib>In, D.S</creatorcontrib><creatorcontrib>Chung, H.J</creatorcontrib><creatorcontrib>Liu, J.R</creatorcontrib><title>Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites</title><title>Plant cell reports</title><addtitle>Plant Cell Rep</addtitle><description>Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment.</description><subject>Acetates - pharmacology</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biosynthesis</subject><subject>Biotechnology</subject><subject>complementary DNA</subject><subject>Cyclopentanes - pharmacology</subject><subject>DNA libraries</subject><subject>DNA, Plant - genetics</subject><subject>Eukaryotic cell cultures</subject><subject>expressed sequence tags</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene expression regulation</subject><subject>Genes, Plant - drug effects</subject><subject>Genetic resources</subject><subject>ginsenoside</subject><subject>Ginsenosides - biosynthesis</subject><subject>Metabolism</subject><subject>Metabolites</subject><subject>Methods. 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Technologies</subject><subject>methyl jasmonate</subject><subject>Miscellaneous</subject><subject>Oxylipins</subject><subject>Panax - drug effects</subject><subject>Panax - genetics</subject><subject>Panax ginseng</subject><subject>Panax pseudoginseng</subject><subject>phytochemicals</subject><subject>Plant cells and fungal cells</subject><subject>plant proteins</subject><subject>Plant Proteins - genetics</subject><subject>Plant Roots - drug effects</subject><subject>Plant Roots - genetics</subject><subject>RNA, Plant - drug effects</subject><subject>RNA, Plant - genetics</subject><subject>root hairs</subject><subject>Roots</subject><subject>Secondary metabolites</subject><subject>sequence analysis</subject><subject>Transcription, Genetic</subject><subject>triterpenoids</subject><issn>0721-7714</issn><issn>1432-203X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><recordid>eNqFkU2LFDEQhhtR3NnVH-BFw8J6a618dCd9XBa_YMGDLngLSTo9k6EnGVOZhf5D_k4zzMCCF08VqKfeIvU0zRsKHyiA_IgAbBhaANGCEl0rnjUrKjhrGfBfz5sVSEZbKam4aC4RtwC1KfuXzQXtOq46KVfNn9to5gUDkjSRkk1El8O-IAmR7HzZLDPZGtylaIpvS_a1jGQdIvq4JhsT8kJySpUviYTRxxKmhax99MeExzQ_VrxGlY0nNiRcYn2dt51SEtYxJCaOJNVeJuhdiqOpwXW_sWkOxeOr5sVkZvSvz_Wqefj86efd1_b--5dvd7f3rROUllbQkU8MLFimpp4O0FkjJ0fFBIYa55mSprNW9gOnhnHLmLXOKDd4A90oHb9q3p9y9zn9PngsehfQ-Xk20acD6l7yTg5S_ReksoNeqSN4_Q-4TYdcj45agRSKMd5XiJ4glxNi9pPe57CrN9AU9FG1PqnWVbU-qtaizrw9Bx_szo9PE2e3Fbg5Awadmacq1wV84noBTPGucu9O3GSSNutcmYcfDCgHOP5UCP4X1qS_Cg</recordid><startdate>2005</startdate><enddate>2005</enddate><creator>Choi, D.W</creator><creator>Jung, J.D</creator><creator>Ha, Y.I</creator><creator>Park, H.W</creator><creator>In, D.S</creator><creator>Chung, H.J</creator><creator>Liu, J.R</creator><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7T5</scope><scope>7T7</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>RC3</scope><scope>7QO</scope><scope>7X8</scope></search><sort><creationdate>2005</creationdate><title>Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites</title><author>Choi, D.W ; Jung, J.D ; Ha, Y.I ; Park, H.W ; In, D.S ; Chung, H.J ; Liu, J.R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c411t-41d3f20b0b28f61905ba7fc14f0a1ace287a5bb76931a23b22bbca8c9ea05d7c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Acetates - pharmacology</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biosynthesis</topic><topic>Biotechnology</topic><topic>complementary DNA</topic><topic>Cyclopentanes - pharmacology</topic><topic>DNA libraries</topic><topic>DNA, Plant - genetics</topic><topic>Eukaryotic cell cultures</topic><topic>expressed sequence tags</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene expression regulation</topic><topic>Genes, Plant - drug effects</topic><topic>Genetic resources</topic><topic>ginsenoside</topic><topic>Ginsenosides - biosynthesis</topic><topic>Metabolism</topic><topic>Metabolites</topic><topic>Methods. Procedures. Technologies</topic><topic>methyl jasmonate</topic><topic>Miscellaneous</topic><topic>Oxylipins</topic><topic>Panax - drug effects</topic><topic>Panax - genetics</topic><topic>Panax ginseng</topic><topic>Panax pseudoginseng</topic><topic>phytochemicals</topic><topic>Plant cells and fungal cells</topic><topic>plant proteins</topic><topic>Plant Proteins - genetics</topic><topic>Plant Roots - drug effects</topic><topic>Plant Roots - genetics</topic><topic>RNA, Plant - drug effects</topic><topic>RNA, Plant - genetics</topic><topic>root hairs</topic><topic>Roots</topic><topic>Secondary metabolites</topic><topic>sequence analysis</topic><topic>Transcription, Genetic</topic><topic>triterpenoids</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Choi, D.W</creatorcontrib><creatorcontrib>Jung, J.D</creatorcontrib><creatorcontrib>Ha, Y.I</creatorcontrib><creatorcontrib>Park, H.W</creatorcontrib><creatorcontrib>In, D.S</creatorcontrib><creatorcontrib>Chung, H.J</creatorcontrib><creatorcontrib>Liu, J.R</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Immunology Abstracts</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Biology Database (Alumni Edition)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>Genetics Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant cell reports</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Choi, D.W</au><au>Jung, J.D</au><au>Ha, Y.I</au><au>Park, H.W</au><au>In, D.S</au><au>Chung, H.J</au><au>Liu, J.R</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites</atitle><jtitle>Plant cell reports</jtitle><addtitle>Plant Cell Rep</addtitle><date>2005</date><risdate>2005</risdate><volume>23</volume><issue>8</issue><spage>557</spage><epage>566</epage><pages>557-566</pages><issn>0721-7714</issn><eissn>1432-203X</eissn><coden>PCRPD8</coden><abstract>Methyl jasmonate (MeJA) treatment increases the levels of plant secondary metabolites, including ginsenosides, which are considered to be the main active compounds in ginseng (Panax ginseng C.A. Meyer). To create a ginseng gene resource that contains the genes involved in the biosynthesis of secondary metabolites, including ginsenosides, we generated 3,134 expression sequence tags (ESTs) from MeJA-treated ginseng hairy roots. These ESTs assembled into 370 clusters and 1,680 singletons. Genes yielding highly abundant transcripts were those encoding proteins involved in fatty acid desaturation, the defense response, and the biosynthesis of secondary metabolites. Analysis of the latter group revealed a number of genes that may be involved in the biosynthesis of ginsenosides, namely, oxidosqualene cyclase (OSC), cytochrome P450, and glycosyltransferase. A novel OSC gene was also identified by this analysis. RNA gel blot analysis confirmed that transcription of this OSC gene, along with squalene synthase (SS) and squalene epoxidase (SE) gene transcription, is increased by MeJA treatment. This ginseng EST data set will also provide important information on the genes that are involved in the biosynthesis of other secondary metabolites and the genes that are responsive to MeJA treatment.</abstract><cop>Berlin</cop><pub>Springer</pub><pmid>15538577</pmid><doi>10.1007/s00299-004-0845-4</doi><tpages>10</tpages></addata></record> |
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| subjects | Acetates - pharmacology Base Sequence Biological and medical sciences Biosynthesis Biotechnology complementary DNA Cyclopentanes - pharmacology DNA libraries DNA, Plant - genetics Eukaryotic cell cultures expressed sequence tags Fundamental and applied biological sciences. Psychology gene expression regulation Genes, Plant - drug effects Genetic resources ginsenoside Ginsenosides - biosynthesis Metabolism Metabolites Methods. Procedures. Technologies methyl jasmonate Miscellaneous Oxylipins Panax - drug effects Panax - genetics Panax ginseng Panax pseudoginseng phytochemicals Plant cells and fungal cells plant proteins Plant Proteins - genetics Plant Roots - drug effects Plant Roots - genetics RNA, Plant - drug effects RNA, Plant - genetics root hairs Roots Secondary metabolites sequence analysis Transcription, Genetic triterpenoids |
| title | Analysis of transcripts in methyl jasmonate-treated ginseng hairy roots to identify genes involved in the biosynthesis of ginsenosides and other secondary metabolites |
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