Quantitative analysis of binding parameters of [ 3H] N-methylscopolamine in central nervous system of muscarinic acetylcholine receptor knockout mice
We have studied binding parameters ( K d, B max) of [ 3H] N-methylscopolamine ([ 3H]NMS) in various brain regions and spinal cord of wild-type (WT) and muscarinic acetylcholine receptor (mAChR) subtype (M 1–M 5) knockout (KO) mice. In the M 1–M 4 KO mice, the number of [ 3H]NMS binding sites ( B max...
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| Veröffentlicht in: | Brain research. Molecular brain research. 2005-01, Vol.133 (1), p.6-11 |
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| creator | Oki, Tomomi Takagi, Yukiko Inagaki, Sayuri Taketo, Makoto M. Manabe, Toshiya Matsui, Minoru Yamada, Shizuo |
| description | We have studied binding parameters (
K
d,
B
max) of [
3H]
N-methylscopolamine ([
3H]NMS) in various brain regions and spinal cord of wild-type (WT) and muscarinic acetylcholine receptor (mAChR) subtype (M
1–M
5) knockout (KO) mice. In the M
1–M
4 KO mice, the number of [
3H]NMS binding sites (
B
max) was decreased throughout the central nervous system (CNS) with significant regional differences. Our results collectively suggest that M
1 receptor was present in a relatively high density in the cerebral cortex and hippocampus, and the densities of M
1 and M
4 subtypes were highest in the corpus striatum. M
2 receptor appeared to be the major subtype in the thalamus, hypothalamus, midbrain, pons-medulla, cerebellum and spinal cord. These findings may contribute significantly not only to the further understanding of the physiological roles of mAChR subtypes in the central cholinergic functions, but also to the development of selective therapeutic agents targeting specific subtype. |
| doi_str_mv | 10.1016/j.molbrainres.2004.09.012 |
| format | Article |
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K
d,
B
max) of [
3H]
N-methylscopolamine ([
3H]NMS) in various brain regions and spinal cord of wild-type (WT) and muscarinic acetylcholine receptor (mAChR) subtype (M
1–M
5) knockout (KO) mice. In the M
1–M
4 KO mice, the number of [
3H]NMS binding sites (
B
max) was decreased throughout the central nervous system (CNS) with significant regional differences. Our results collectively suggest that M
1 receptor was present in a relatively high density in the cerebral cortex and hippocampus, and the densities of M
1 and M
4 subtypes were highest in the corpus striatum. M
2 receptor appeared to be the major subtype in the thalamus, hypothalamus, midbrain, pons-medulla, cerebellum and spinal cord. These findings may contribute significantly not only to the further understanding of the physiological roles of mAChR subtypes in the central cholinergic functions, but also to the development of selective therapeutic agents targeting specific subtype.</description><identifier>ISSN: 0169-328X</identifier><identifier>EISSN: 1872-6941</identifier><identifier>DOI: 10.1016/j.molbrainres.2004.09.012</identifier><identifier>PMID: 15661360</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Central Nervous System - metabolism ; CNS distribution ; Dose-Response Relationship, Drug ; Fundamental and applied biological sciences. Psychology ; Knockout mice ; Mice ; Mice, Knockout - metabolism ; Muscarinic acetylcholine receptor subtype ; N-Methylscopolamine - pharmacokinetics ; Parasympatholytics - pharmacokinetics ; Protein Binding ; Radioligand Assay - methods ; Receptors, Muscarinic - classification ; Receptors, Muscarinic - genetics ; Receptors, Muscarinic - metabolism ; Tissue Distribution ; Tritium ; Vertebrates: nervous system and sense organs</subject><ispartof>Brain research. Molecular brain research., 2005-01, Vol.133 (1), p.6-11</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c502t-24403b9c02278275cb017d6b23bd806c9a3d2d1bfcb519267b1debf68445d7993</citedby><cites>FETCH-LOGICAL-c502t-24403b9c02278275cb017d6b23bd806c9a3d2d1bfcb519267b1debf68445d7993</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16460872$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15661360$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Oki, Tomomi</creatorcontrib><creatorcontrib>Takagi, Yukiko</creatorcontrib><creatorcontrib>Inagaki, Sayuri</creatorcontrib><creatorcontrib>Taketo, Makoto M.</creatorcontrib><creatorcontrib>Manabe, Toshiya</creatorcontrib><creatorcontrib>Matsui, Minoru</creatorcontrib><creatorcontrib>Yamada, Shizuo</creatorcontrib><title>Quantitative analysis of binding parameters of [ 3H] N-methylscopolamine in central nervous system of muscarinic acetylcholine receptor knockout mice</title><title>Brain research. Molecular brain research.</title><addtitle>Brain Res Mol Brain Res</addtitle><description>We have studied binding parameters (
K
d,
B
max) of [
3H]
N-methylscopolamine ([
3H]NMS) in various brain regions and spinal cord of wild-type (WT) and muscarinic acetylcholine receptor (mAChR) subtype (M
1–M
5) knockout (KO) mice. In the M
1–M
4 KO mice, the number of [
3H]NMS binding sites (
B
max) was decreased throughout the central nervous system (CNS) with significant regional differences. Our results collectively suggest that M
1 receptor was present in a relatively high density in the cerebral cortex and hippocampus, and the densities of M
1 and M
4 subtypes were highest in the corpus striatum. M
2 receptor appeared to be the major subtype in the thalamus, hypothalamus, midbrain, pons-medulla, cerebellum and spinal cord. These findings may contribute significantly not only to the further understanding of the physiological roles of mAChR subtypes in the central cholinergic functions, but also to the development of selective therapeutic agents targeting specific subtype.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Central Nervous System - metabolism</subject><subject>CNS distribution</subject><subject>Dose-Response Relationship, Drug</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Knockout mice</subject><subject>Mice</subject><subject>Mice, Knockout - metabolism</subject><subject>Muscarinic acetylcholine receptor subtype</subject><subject>N-Methylscopolamine - pharmacokinetics</subject><subject>Parasympatholytics - pharmacokinetics</subject><subject>Protein Binding</subject><subject>Radioligand Assay - methods</subject><subject>Receptors, Muscarinic - classification</subject><subject>Receptors, Muscarinic - genetics</subject><subject>Receptors, Muscarinic - metabolism</subject><subject>Tissue Distribution</subject><subject>Tritium</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0169-328X</issn><issn>1872-6941</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqNkdGK1DAUhoMo7rj6ChIv9K5jkrZpcynD6gqLIigIIiFJT93MpklN0oE-iO9rxhlY7_QqcPj-JOf_EHpByZYSyl_vt1NwOirrI6QtI6TZErEllD1AG9p3rOKioQ_RprCiqln_9QI9SWlPCKE9pY_RBW05pzUnG_Tr06J8tlllewCsvHJrsgmHEWvrB-t_4FlFNUGG-Gf6DdfX3_GHqkxuV5dMmINTk_WArccGfI7KYQ_xEJaE05oyTMfYtCSjovXWYGUgr87cBndMRTAw5xDxnQ_mLiwZT9bAU_RoVC7Bs_N5ib68vfq8u65uPr57v3tzU5mWsFyxpiG1FoYw1vWsa40mtBu4ZrUeesKNUPXABqpHo1sqGO80HUCPvG-aduiEqC_Rq9O9cww_F0hZTjYZcE55KAtI3tVt09b0nyDt2q7uGSugOIEmhpQijHKOdlJxlZTIozy5l3_Jk0d5kghZ5JXs8_Mji55guE-ebRXg5RlQpU43RuWNTfccbzgp-gu3O3FQujtYiDIZC97AYEvfWQ7B_sd3fgOplcG5</recordid><startdate>20050105</startdate><enddate>20050105</enddate><creator>Oki, Tomomi</creator><creator>Takagi, Yukiko</creator><creator>Inagaki, Sayuri</creator><creator>Taketo, Makoto M.</creator><creator>Manabe, Toshiya</creator><creator>Matsui, Minoru</creator><creator>Yamada, Shizuo</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>20050105</creationdate><title>Quantitative analysis of binding parameters of [ 3H] N-methylscopolamine in central nervous system of muscarinic acetylcholine receptor knockout mice</title><author>Oki, Tomomi ; Takagi, Yukiko ; Inagaki, Sayuri ; Taketo, Makoto M. ; Manabe, Toshiya ; Matsui, Minoru ; Yamada, Shizuo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c502t-24403b9c02278275cb017d6b23bd806c9a3d2d1bfcb519267b1debf68445d7993</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Central Nervous System - metabolism</topic><topic>CNS distribution</topic><topic>Dose-Response Relationship, Drug</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Knockout mice</topic><topic>Mice</topic><topic>Mice, Knockout - metabolism</topic><topic>Muscarinic acetylcholine receptor subtype</topic><topic>N-Methylscopolamine - pharmacokinetics</topic><topic>Parasympatholytics - pharmacokinetics</topic><topic>Protein Binding</topic><topic>Radioligand Assay - methods</topic><topic>Receptors, Muscarinic - classification</topic><topic>Receptors, Muscarinic - genetics</topic><topic>Receptors, Muscarinic - metabolism</topic><topic>Tissue Distribution</topic><topic>Tritium</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Oki, Tomomi</creatorcontrib><creatorcontrib>Takagi, Yukiko</creatorcontrib><creatorcontrib>Inagaki, Sayuri</creatorcontrib><creatorcontrib>Taketo, Makoto M.</creatorcontrib><creatorcontrib>Manabe, Toshiya</creatorcontrib><creatorcontrib>Matsui, Minoru</creatorcontrib><creatorcontrib>Yamada, Shizuo</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Brain research. Molecular brain research.</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Oki, Tomomi</au><au>Takagi, Yukiko</au><au>Inagaki, Sayuri</au><au>Taketo, Makoto M.</au><au>Manabe, Toshiya</au><au>Matsui, Minoru</au><au>Yamada, Shizuo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Quantitative analysis of binding parameters of [ 3H] N-methylscopolamine in central nervous system of muscarinic acetylcholine receptor knockout mice</atitle><jtitle>Brain research. Molecular brain research.</jtitle><addtitle>Brain Res Mol Brain Res</addtitle><date>2005-01-05</date><risdate>2005</risdate><volume>133</volume><issue>1</issue><spage>6</spage><epage>11</epage><pages>6-11</pages><issn>0169-328X</issn><eissn>1872-6941</eissn><abstract>We have studied binding parameters (
K
d,
B
max) of [
3H]
N-methylscopolamine ([
3H]NMS) in various brain regions and spinal cord of wild-type (WT) and muscarinic acetylcholine receptor (mAChR) subtype (M
1–M
5) knockout (KO) mice. In the M
1–M
4 KO mice, the number of [
3H]NMS binding sites (
B
max) was decreased throughout the central nervous system (CNS) with significant regional differences. Our results collectively suggest that M
1 receptor was present in a relatively high density in the cerebral cortex and hippocampus, and the densities of M
1 and M
4 subtypes were highest in the corpus striatum. M
2 receptor appeared to be the major subtype in the thalamus, hypothalamus, midbrain, pons-medulla, cerebellum and spinal cord. These findings may contribute significantly not only to the further understanding of the physiological roles of mAChR subtypes in the central cholinergic functions, but also to the development of selective therapeutic agents targeting specific subtype.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15661360</pmid><doi>10.1016/j.molbrainres.2004.09.012</doi><tpages>6</tpages></addata></record> |
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| ispartof | Brain research. Molecular brain research., 2005-01, Vol.133 (1), p.6-11 |
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| subjects | Animals Biological and medical sciences Central Nervous System - metabolism CNS distribution Dose-Response Relationship, Drug Fundamental and applied biological sciences. Psychology Knockout mice Mice Mice, Knockout - metabolism Muscarinic acetylcholine receptor subtype N-Methylscopolamine - pharmacokinetics Parasympatholytics - pharmacokinetics Protein Binding Radioligand Assay - methods Receptors, Muscarinic - classification Receptors, Muscarinic - genetics Receptors, Muscarinic - metabolism Tissue Distribution Tritium Vertebrates: nervous system and sense organs |
| title | Quantitative analysis of binding parameters of [ 3H] N-methylscopolamine in central nervous system of muscarinic acetylcholine receptor knockout mice |
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