Down-regulation of miR-141 in gastric cancer and its involvement in cell growth
Purpose Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer...
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| Veröffentlicht in: | Journal of gastroenterology 2009-06, Vol.44 (6), p.556-561 |
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| creator | Du, Ying Xu, Yanjun Ding, Ling Yao, Haomi Yu, Hong Zhou, Tianhua Si, Jianmin |
| description | Purpose Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. Methods The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. Results MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. Conclusions These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation. |
| doi_str_mv | 10.1007/s00535-009-0037-7 |
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However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. Methods The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. Results MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. Conclusions These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.</description><identifier>ISSN: 0944-1174</identifier><identifier>EISSN: 1435-5922</identifier><identifier>DOI: 10.1007/s00535-009-0037-7</identifier><identifier>PMID: 19363643</identifier><language>eng</language><publisher>Japan: Japan : Springer Japan</publisher><subject>Abdominal Surgery ; Aged ; Aged, 80 and over ; Cell Proliferation ; Colorectal Surgery ; Down-Regulation - physiology ; Female ; Gastroenterology ; Gene Expression Regulation, Neoplastic - physiology ; Hepatology ; Humans ; Male ; Medicine ; Medicine & Public Health ; MicroRNAs - metabolism ; MicroRNAs - physiology ; Middle Aged ; Original Article—Alimentary Tract ; Stomach Neoplasms - genetics ; Stomach Neoplasms - pathology ; Stomach Neoplasms - physiopathology ; Surgical Oncology ; Tumor Cells, Cultured</subject><ispartof>Journal of gastroenterology, 2009-06, Vol.44 (6), p.556-561</ispartof><rights>Springer 2009</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c512t-390cc5673cf3a5f3c1a1af411aba27ad9561cd45066af8fe03381ef1cbd347dd3</citedby><cites>FETCH-LOGICAL-c512t-390cc5673cf3a5f3c1a1af411aba27ad9561cd45066af8fe03381ef1cbd347dd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1007/s00535-009-0037-7$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1007/s00535-009-0037-7$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19363643$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Du, Ying</creatorcontrib><creatorcontrib>Xu, Yanjun</creatorcontrib><creatorcontrib>Ding, Ling</creatorcontrib><creatorcontrib>Yao, Haomi</creatorcontrib><creatorcontrib>Yu, Hong</creatorcontrib><creatorcontrib>Zhou, Tianhua</creatorcontrib><creatorcontrib>Si, Jianmin</creatorcontrib><title>Down-regulation of miR-141 in gastric cancer and its involvement in cell growth</title><title>Journal of gastroenterology</title><addtitle>J Gastroenterol</addtitle><addtitle>J Gastroenterol</addtitle><description>Purpose Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. Methods The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. Results MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. Conclusions These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.</description><subject>Abdominal Surgery</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Cell Proliferation</subject><subject>Colorectal Surgery</subject><subject>Down-Regulation - physiology</subject><subject>Female</subject><subject>Gastroenterology</subject><subject>Gene Expression Regulation, Neoplastic - physiology</subject><subject>Hepatology</subject><subject>Humans</subject><subject>Male</subject><subject>Medicine</subject><subject>Medicine & Public Health</subject><subject>MicroRNAs - metabolism</subject><subject>MicroRNAs - physiology</subject><subject>Middle Aged</subject><subject>Original Article—Alimentary Tract</subject><subject>Stomach Neoplasms - genetics</subject><subject>Stomach Neoplasms - pathology</subject><subject>Stomach Neoplasms - physiopathology</subject><subject>Surgical Oncology</subject><subject>Tumor Cells, Cultured</subject><issn>0944-1174</issn><issn>1435-5922</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><recordid>eNp9kE1rFTEUhoMo9vbqD3Cjg4vuRs_J581S6icUCmrXITeTjFNmkprMtPjvzTAXCi5chEDOc968PIS8QniHAOp9ARBMtAC6HqZa9YTskNcXoSl9SnagOW8RFT8j56XcAiADcXhOzlAzySRnO3L9MT3ENvt-Ge08pNik0EzD9xY5NkNselvmPLjG2eh8bmzsmmEudXKfxns_-TivlPPj2PQ5Pcy_XpBnwY7Fvzzde3Lz-dPPy6_t1fWXb5cfrlonkM4t0-CckIq5wKwIzKFFGziiPVqqbKeFRNdxAVLacAgeGDugD-iOHeOq69ieXGy5dzn9XnyZzTSUtYeNPi3F1GhBNdUVfPsPeJuWHGs3Q1GhlLRa2RPcIJdTKdkHc5eHyeY_BsGsqs2m2lTVZlVtVN15fQpejpPvHjdObitAN6DUUex9fvz5f6lvtqVgk7F9Hoq5-bFWBJScatDsL0T5kS4</recordid><startdate>20090601</startdate><enddate>20090601</enddate><creator>Du, Ying</creator><creator>Xu, Yanjun</creator><creator>Ding, Ling</creator><creator>Yao, Haomi</creator><creator>Yu, Hong</creator><creator>Zhou, Tianhua</creator><creator>Si, Jianmin</creator><general>Japan : Springer Japan</general><general>Springer Japan</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7RV</scope><scope>7T5</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>K9-</scope><scope>K9.</scope><scope>KB0</scope><scope>M0R</scope><scope>M0S</scope><scope>M1P</scope><scope>NAPCQ</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>8BM</scope></search><sort><creationdate>20090601</creationdate><title>Down-regulation of miR-141 in gastric cancer and its involvement in cell growth</title><author>Du, Ying ; Xu, Yanjun ; Ding, Ling ; Yao, Haomi ; Yu, Hong ; Zhou, Tianhua ; Si, Jianmin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c512t-390cc5673cf3a5f3c1a1af411aba27ad9561cd45066af8fe03381ef1cbd347dd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2009</creationdate><topic>Abdominal Surgery</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Cell Proliferation</topic><topic>Colorectal Surgery</topic><topic>Down-Regulation - physiology</topic><topic>Female</topic><topic>Gastroenterology</topic><topic>Gene Expression Regulation, Neoplastic - physiology</topic><topic>Hepatology</topic><topic>Humans</topic><topic>Male</topic><topic>Medicine</topic><topic>Medicine & Public Health</topic><topic>MicroRNAs - metabolism</topic><topic>MicroRNAs - physiology</topic><topic>Middle Aged</topic><topic>Original Article—Alimentary Tract</topic><topic>Stomach Neoplasms - genetics</topic><topic>Stomach Neoplasms - pathology</topic><topic>Stomach Neoplasms - physiopathology</topic><topic>Surgical Oncology</topic><topic>Tumor Cells, Cultured</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Du, Ying</creatorcontrib><creatorcontrib>Xu, Yanjun</creatorcontrib><creatorcontrib>Ding, Ling</creatorcontrib><creatorcontrib>Yao, Haomi</creatorcontrib><creatorcontrib>Yu, Hong</creatorcontrib><creatorcontrib>Zhou, Tianhua</creatorcontrib><creatorcontrib>Si, Jianmin</creatorcontrib><collection>AGRIS</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Nursing & Allied Health Database</collection><collection>Immunology Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Consumer Health Database (Alumni Edition)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Consumer Health Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Nursing & Allied Health Premium</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>ComDisDome</collection><jtitle>Journal of gastroenterology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Du, Ying</au><au>Xu, Yanjun</au><au>Ding, Ling</au><au>Yao, Haomi</au><au>Yu, Hong</au><au>Zhou, Tianhua</au><au>Si, Jianmin</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Down-regulation of miR-141 in gastric cancer and its involvement in cell growth</atitle><jtitle>Journal of gastroenterology</jtitle><stitle>J Gastroenterol</stitle><addtitle>J Gastroenterol</addtitle><date>2009-06-01</date><risdate>2009</risdate><volume>44</volume><issue>6</issue><spage>556</spage><epage>561</epage><pages>556-561</pages><issn>0944-1174</issn><eissn>1435-5922</eissn><abstract>Purpose Human microRNA-141 (miR-141), a member of the miR-200 family, has been reported to be associated with various human malignancies. However, it remains unknown whether miR-141 is involved in the pathogenesis of gastric cancer. Therefore, we examined the expression of miR-141 in gastric cancer tissues and the effect of miR-141 overexpression on cancer cell proliferation. Methods The expression level of miR-141 in 35 pair-matched gastric neoplastic and adjacent non-neoplastic tissues, and in 5 gastric cancer cell lines were examined by quantitative real-time PCR. The growth of MGC-803 cells transfected with miRNA precursor was examined by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazoliumbromide) assay. Results MiR-141 was significantly down-regulated in 80% (28/35) of primary gastric cancer tissues compared with pair-matched adjacent non-tumor tissues (P < 0.01). The expression of miR-141 was also found to be substantially reduced in several human gastric cancer cell lines such as MGC-803, HGC-27, SGC-7901 and BGC-823 cells. Overexpression of miR-141 with its precursors significantly inhibited the proliferation of gastric cancer cells. Conclusions These results suggest that miR-141 may be involved in the development of gastric cancer through its inhibitory effect on cell proliferation.</abstract><cop>Japan</cop><pub>Japan : Springer Japan</pub><pmid>19363643</pmid><doi>10.1007/s00535-009-0037-7</doi><tpages>6</tpages></addata></record> |
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| subjects | Abdominal Surgery Aged Aged, 80 and over Cell Proliferation Colorectal Surgery Down-Regulation - physiology Female Gastroenterology Gene Expression Regulation, Neoplastic - physiology Hepatology Humans Male Medicine Medicine & Public Health MicroRNAs - metabolism MicroRNAs - physiology Middle Aged Original Article—Alimentary Tract Stomach Neoplasms - genetics Stomach Neoplasms - pathology Stomach Neoplasms - physiopathology Surgical Oncology Tumor Cells, Cultured |
| title | Down-regulation of miR-141 in gastric cancer and its involvement in cell growth |
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