relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera
Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to the vertebrate luteinizing hormone (LH). Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determ...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 2009-06, Vol.106 (23), p.9507-9512 |
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| creator | Mita, Masatoshi Yoshikuni, Michiyasu Ohno, Kaoru Shibata, Yasushi Paul-Prasanth, Bindhu Pitchayawasin, Suthasinee Isobe, Minoru Nagahama, Yoshitaka |
| description | Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to the vertebrate luteinizing hormone (LH). Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determined its amino acid sequence. The purified GSS was a heterodimer composed of 2 different peptides, A and B chains, with disulfide cross-linkages. Based on its cysteine motif, starfish GSS was classified as a member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily. The cDNA of GSS encodes a preprohormone sequence with a C peptide between the A and B chains. Phylogenetic analyses revealed that starfish GSS was a relaxin-like peptide. Chemically synthesized GSS induced not only oocyte maturation and ovulation in isolated ovarian fragments, but also unique spawning behavior, followed by release of gametes shortly after the injection. Importantly, the action of the synthetic GSS on oocyte maturation and ovulation was mediated through the production of cAMP by isolated ovarian follicle cells, thereby producing the maturation-inducing hormone of this species, 1-methyladenine. In situ hybridization showed the transcription of GSS to occur in the periphery of radial nerves at the side of tube feet. Together, the structure, sequence, and mode of signal transduction strongly suggest that GSS is closely related to the vertebrate relaxin. |
| doi_str_mv | 10.1073/pnas.0900243106 |
| format | Article |
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Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determined its amino acid sequence. The purified GSS was a heterodimer composed of 2 different peptides, A and B chains, with disulfide cross-linkages. Based on its cysteine motif, starfish GSS was classified as a member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily. The cDNA of GSS encodes a preprohormone sequence with a C peptide between the A and B chains. Phylogenetic analyses revealed that starfish GSS was a relaxin-like peptide. Chemically synthesized GSS induced not only oocyte maturation and ovulation in isolated ovarian fragments, but also unique spawning behavior, followed by release of gametes shortly after the injection. Importantly, the action of the synthetic GSS on oocyte maturation and ovulation was mediated through the production of cAMP by isolated ovarian follicle cells, thereby producing the maturation-inducing hormone of this species, 1-methyladenine. In situ hybridization showed the transcription of GSS to occur in the periphery of radial nerves at the side of tube feet. Together, the structure, sequence, and mode of signal transduction strongly suggest that GSS is closely related to the vertebrate relaxin.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.0900243106</identifier><identifier>PMID: 19470645</identifier><language>eng</language><publisher>United States: National Academy of Sciences</publisher><subject>Amino Acid Sequence ; Amino acids ; Animals ; Asterina - chemistry ; Asterina - physiology ; Asterina pectinifera ; Asteroidea ; Base Sequence ; Biological Sciences ; Complementary DNA ; Female ; Fish ; Fluorescence in situ hybridization ; Gene Expression ; Hormones ; Insulin ; Invertebrate Hormones - chemistry ; Invertebrate Hormones - genetics ; Invertebrate Hormones - metabolism ; Marine ; Maturation ; Molecular Sequence Data ; Nerves ; Neuropeptides - chemistry ; Neuropeptides - genetics ; Neuropeptides - metabolism ; Oocytes ; Oogenesis ; Ovulation ; Peptides ; Phylogenetics ; Radial nerve ; Signal transduction ; Starfish ; Vertebrates</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 2009-06, Vol.106 (23), p.9507-9512</ispartof><rights>Copyright National Academy of Sciences Jun 9, 2009</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c618t-603bf46561bad75e68a239f3d77cd855e6e3741e833390cb2a51baca9587456d3</citedby><cites>FETCH-LOGICAL-c618t-603bf46561bad75e68a239f3d77cd855e6e3741e833390cb2a51baca9587456d3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/106/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/40483245$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/40483245$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/19470645$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mita, Masatoshi</creatorcontrib><creatorcontrib>Yoshikuni, Michiyasu</creatorcontrib><creatorcontrib>Ohno, Kaoru</creatorcontrib><creatorcontrib>Shibata, Yasushi</creatorcontrib><creatorcontrib>Paul-Prasanth, Bindhu</creatorcontrib><creatorcontrib>Pitchayawasin, Suthasinee</creatorcontrib><creatorcontrib>Isobe, Minoru</creatorcontrib><creatorcontrib>Nagahama, Yoshitaka</creatorcontrib><title>relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Gonad-stimulating substance (GSS) of starfish is the only known invertebrate peptide hormone responsible for final gamete maturation, rendering it functionally analogous to the vertebrate luteinizing hormone (LH). Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determined its amino acid sequence. The purified GSS was a heterodimer composed of 2 different peptides, A and B chains, with disulfide cross-linkages. Based on its cysteine motif, starfish GSS was classified as a member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily. The cDNA of GSS encodes a preprohormone sequence with a C peptide between the A and B chains. Phylogenetic analyses revealed that starfish GSS was a relaxin-like peptide. Chemically synthesized GSS induced not only oocyte maturation and ovulation in isolated ovarian fragments, but also unique spawning behavior, followed by release of gametes shortly after the injection. Importantly, the action of the synthetic GSS on oocyte maturation and ovulation was mediated through the production of cAMP by isolated ovarian follicle cells, thereby producing the maturation-inducing hormone of this species, 1-methyladenine. In situ hybridization showed the transcription of GSS to occur in the periphery of radial nerves at the side of tube feet. Together, the structure, sequence, and mode of signal transduction strongly suggest that GSS is closely related to the vertebrate relaxin.</description><subject>Amino Acid Sequence</subject><subject>Amino acids</subject><subject>Animals</subject><subject>Asterina - chemistry</subject><subject>Asterina - physiology</subject><subject>Asterina pectinifera</subject><subject>Asteroidea</subject><subject>Base Sequence</subject><subject>Biological Sciences</subject><subject>Complementary DNA</subject><subject>Female</subject><subject>Fish</subject><subject>Fluorescence in situ hybridization</subject><subject>Gene Expression</subject><subject>Hormones</subject><subject>Insulin</subject><subject>Invertebrate Hormones - chemistry</subject><subject>Invertebrate Hormones - genetics</subject><subject>Invertebrate Hormones - metabolism</subject><subject>Marine</subject><subject>Maturation</subject><subject>Molecular Sequence Data</subject><subject>Nerves</subject><subject>Neuropeptides - chemistry</subject><subject>Neuropeptides - genetics</subject><subject>Neuropeptides - metabolism</subject><subject>Oocytes</subject><subject>Oogenesis</subject><subject>Ovulation</subject><subject>Peptides</subject><subject>Phylogenetics</subject><subject>Radial nerve</subject><subject>Signal transduction</subject><subject>Starfish</subject><subject>Vertebrates</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2009</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1vEzEQhlcIREPhzAmwOCAObDv-3r0gVRVfUiUO0LPl7M42Dhs72N6ovfS311GipnCA08jjZ96Z156qeknhhILmp2tv0wm0AExwCupRNaPQ0lqJFh5Xs5LWdSOYOKqepbQEgFY28LQ6oq3QoIScVbcRR3vtfD26X0jWuM6uL3GKbnDYkyGGFYm2d3YkHuMGE3G-n7oSQ-huMpKVzVO02QVPrO9J2Ezj7uQ8yQskKds4uLT4QM5Sxui8LV267LwbMNrn1ZPBjglf7ONxdfn508_zr_XF9y_fzs8u6k7RJtcK-HwQSio6t72WqBrLeDvwXuuub2RJINeCYsM5b6GbMysL2dliVwupen5cfdzprqf5CvsOfY52NOvoVjbemGCd-fPGu4W5ChvDVCOZpEXg3V4ght8TpmxWLnU4jtZjmJJRmkvWCP1fkEFDQei2gG__Apdhir68QmGoAKaoKNDpDupiSCnicD8yBbPdALPdAHPYgFLx-qHTA7__8gK82QPbyoOcMoybVsLWwvt_E2aYxjHjdS7oqx26TDnEe1aAaDh72Gywwdir6JK5_FHscaBKsDIPvwM1NtoQ</recordid><startdate>20090609</startdate><enddate>20090609</enddate><creator>Mita, Masatoshi</creator><creator>Yoshikuni, Michiyasu</creator><creator>Ohno, Kaoru</creator><creator>Shibata, Yasushi</creator><creator>Paul-Prasanth, Bindhu</creator><creator>Pitchayawasin, Suthasinee</creator><creator>Isobe, Minoru</creator><creator>Nagahama, Yoshitaka</creator><general>National Academy of Sciences</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7TN</scope><scope>F1W</scope><scope>H95</scope><scope>H98</scope><scope>L.G</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>20090609</creationdate><title>relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera</title><author>Mita, Masatoshi ; 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Here, we purified GSS of starfish, Asterina pectinifera, from radial nerves and determined its amino acid sequence. The purified GSS was a heterodimer composed of 2 different peptides, A and B chains, with disulfide cross-linkages. Based on its cysteine motif, starfish GSS was classified as a member of the insulin/insulin-like growth factor (IGF)/relaxin superfamily. The cDNA of GSS encodes a preprohormone sequence with a C peptide between the A and B chains. Phylogenetic analyses revealed that starfish GSS was a relaxin-like peptide. Chemically synthesized GSS induced not only oocyte maturation and ovulation in isolated ovarian fragments, but also unique spawning behavior, followed by release of gametes shortly after the injection. Importantly, the action of the synthetic GSS on oocyte maturation and ovulation was mediated through the production of cAMP by isolated ovarian follicle cells, thereby producing the maturation-inducing hormone of this species, 1-methyladenine. In situ hybridization showed the transcription of GSS to occur in the periphery of radial nerves at the side of tube feet. Together, the structure, sequence, and mode of signal transduction strongly suggest that GSS is closely related to the vertebrate relaxin.</abstract><cop>United States</cop><pub>National Academy of Sciences</pub><pmid>19470645</pmid><doi>10.1073/pnas.0900243106</doi><tpages>6</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence Amino acids Animals Asterina - chemistry Asterina - physiology Asterina pectinifera Asteroidea Base Sequence Biological Sciences Complementary DNA Female Fish Fluorescence in situ hybridization Gene Expression Hormones Insulin Invertebrate Hormones - chemistry Invertebrate Hormones - genetics Invertebrate Hormones - metabolism Marine Maturation Molecular Sequence Data Nerves Neuropeptides - chemistry Neuropeptides - genetics Neuropeptides - metabolism Oocytes Oogenesis Ovulation Peptides Phylogenetics Radial nerve Signal transduction Starfish Vertebrates |
| title | relaxin-like peptide purified from radial nerves induces oocyte maturation and ovulation in the starfish, Asterina pectinifera |
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