Production and secretion of recombinant Leuconostoc mesenteroides dextransucrase DsrS in Bacillus megaterium

Leuconostoc mesenteroides dextransucrase DsrS was recombinantly produced in Bacillus megaterium and exported into the growth medium. For this purpose a plasmid‐based xylose‐inducible gene expression system was optimized via introduction of a multiple cloning site and an encoded optimal B. megaterium...

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Veröffentlicht in:	Biotechnology and bioengineering 2005-01, Vol.89 (2), p.206-218
Hauptverfasser:	Malten, Marco, Hollmann, Rajan, Deckwer, Wolf-Dieter, Jahn, Dieter
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacillus megaterium Bacillus megaterium - enzymology Bacillus megaterium - genetics Bacteria Biological and medical sciences Biotechnology dextransucrase Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - physiology Gene Expression Regulation, Enzymologic - physiology Genetic engineering Genetic Enhancement - methods Glucosyltransferases - biosynthesis Glucosyltransferases - genetics heterologous gene expression high cell density cultivation Leuconostoc - enzymology Leuconostoc - genetics Leuconostoc mesenteroides Protein Engineering - methods Recombinant Proteins - biosynthesis secretion Transformation, Bacterial - genetics
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