Growth factor-loaded scaffolds for bone engineering
The objective of the study presented here was to investigate the bone inductive properties as well as release kinetics of rhTGF-β1- and rhBMP-2-loaded Ti-fiber mesh and CaP cement scaffolds. Therefore, Ti-fiber mesh and porous CaP cement scaffolds were provided with these growth factors and inserted...
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| Veröffentlicht in: | Journal of controlled release 2005-01, Vol.101 (1), p.127-136 |
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| creator | Jansen, J.A. Vehof, J.W.M. Ruhé, P.Q. Kroeze-Deutman, H. Kuboki, Y. Takita, H. Hedberg, E.L. Mikos, A.G. |
| description | The objective of the study presented here was to investigate the bone inductive properties as well as release kinetics of rhTGF-β1- and rhBMP-2-loaded Ti-fiber mesh and CaP cement scaffolds. Therefore, Ti-fiber mesh and porous CaP cement scaffolds were provided with these growth factors and inserted in subcutaneous and cranial implant locations in rats and rabbits.
In vitro, a rapid release of rhTGF-β
1 was observed during the first 2 h of the Ti-fiber mesh scaffolds. During this time, more than 50% of the total dose of rhTGF-β
1 was released. Following this initial peak, a decline in the level of rhTGF-β
1 occurred. After 1 week, the entire theoretical initial dose was observed to have been released. This in contrast to the rhTGF-β
1 and rhBMP-2 release of the porous CaP cement scaffolds. Here, no substantial initial burst release was observed. The scaffolds showed an initial release of about 1% after 1 day, followed by an additional marginal release after 1 week.
Histological analysis revealed excellent osteoconductive properties of non-loaded Ca-P material. Inside non-loaded Ti-mesh fiber scaffolds, also bone ingrowth occurred. Quantification of the bone ingrowth showed that bone formation was increased significantly in all scaffold materials by administration of rhTGF-β
1 and rhBMP-2.
Consequently, we conclude that the release kinetics of growth factors from porous CaP cement differs from other scaffold materials, like metals and polymers. Nevertheless, orthotopic bone formation in a rabbit cranial defect model was stimulated in rhTGF-β
1- and rhBMP-2-loaded CaP cement and Ti-fiber mesh scaffolds compared with non-loaded implants. |
| doi_str_mv | 10.1016/j.jconrel.2004.07.005 |
| format | Article |
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In vitro, a rapid release of rhTGF-β
1 was observed during the first 2 h of the Ti-fiber mesh scaffolds. During this time, more than 50% of the total dose of rhTGF-β
1 was released. Following this initial peak, a decline in the level of rhTGF-β
1 occurred. After 1 week, the entire theoretical initial dose was observed to have been released. This in contrast to the rhTGF-β
1 and rhBMP-2 release of the porous CaP cement scaffolds. Here, no substantial initial burst release was observed. The scaffolds showed an initial release of about 1% after 1 day, followed by an additional marginal release after 1 week.
Histological analysis revealed excellent osteoconductive properties of non-loaded Ca-P material. Inside non-loaded Ti-mesh fiber scaffolds, also bone ingrowth occurred. Quantification of the bone ingrowth showed that bone formation was increased significantly in all scaffold materials by administration of rhTGF-β
1 and rhBMP-2.
Consequently, we conclude that the release kinetics of growth factors from porous CaP cement differs from other scaffold materials, like metals and polymers. Nevertheless, orthotopic bone formation in a rabbit cranial defect model was stimulated in rhTGF-β
1- and rhBMP-2-loaded CaP cement and Ti-fiber mesh scaffolds compared with non-loaded implants.</description><identifier>ISSN: 0168-3659</identifier><identifier>EISSN: 1873-4995</identifier><identifier>DOI: 10.1016/j.jconrel.2004.07.005</identifier><identifier>PMID: 15588899</identifier><identifier>CODEN: JCREEC</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Animals ; Biological and medical sciences ; Bone Cements ; Bone engineering ; Bone Morphogenetic Protein 2 ; Bone Morphogenetic Proteins - administration & dosage ; Bone Morphogenetic Proteins - secretion ; Calcium phosphate cement ; Calcium Phosphates ; Female ; General pharmacology ; Male ; Medical sciences ; Osteogenesis - drug effects ; Osteoinduction ; Pharmaceutical technology. Pharmaceutical industry ; Pharmacology. Drug treatments ; Rabbits ; Rats ; Rats, Wistar ; Recombinant human BMP-2 ; Recombinant Proteins - administration & dosage ; Tissue Engineering ; Titanium - administration & dosage ; Titanium fiber mesh ; Transforming Growth Factor beta - administration & dosage ; Transforming Growth Factor beta - secretion ; Transforming growth factor beta-1 ; Transforming Growth Factor beta1</subject><ispartof>Journal of controlled release, 2005-01, Vol.101 (1), p.127-136</ispartof><rights>2004 Elsevier B.V.</rights><rights>2005 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-eae8f81031c848d6db75297d2c2dfb577f4a71e166c2460672780e403a687b123</citedby><cites>FETCH-LOGICAL-c490t-eae8f81031c848d6db75297d2c2dfb577f4a71e166c2460672780e403a687b123</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.jconrel.2004.07.005$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>309,310,314,780,784,789,790,3550,23930,23931,25140,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=16356115$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/15588899$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Jansen, J.A.</creatorcontrib><creatorcontrib>Vehof, J.W.M.</creatorcontrib><creatorcontrib>Ruhé, P.Q.</creatorcontrib><creatorcontrib>Kroeze-Deutman, H.</creatorcontrib><creatorcontrib>Kuboki, Y.</creatorcontrib><creatorcontrib>Takita, H.</creatorcontrib><creatorcontrib>Hedberg, E.L.</creatorcontrib><creatorcontrib>Mikos, A.G.</creatorcontrib><title>Growth factor-loaded scaffolds for bone engineering</title><title>Journal of controlled release</title><addtitle>J Control Release</addtitle><description>The objective of the study presented here was to investigate the bone inductive properties as well as release kinetics of rhTGF-β1- and rhBMP-2-loaded Ti-fiber mesh and CaP cement scaffolds. Therefore, Ti-fiber mesh and porous CaP cement scaffolds were provided with these growth factors and inserted in subcutaneous and cranial implant locations in rats and rabbits.
In vitro, a rapid release of rhTGF-β
1 was observed during the first 2 h of the Ti-fiber mesh scaffolds. During this time, more than 50% of the total dose of rhTGF-β
1 was released. Following this initial peak, a decline in the level of rhTGF-β
1 occurred. After 1 week, the entire theoretical initial dose was observed to have been released. This in contrast to the rhTGF-β
1 and rhBMP-2 release of the porous CaP cement scaffolds. Here, no substantial initial burst release was observed. The scaffolds showed an initial release of about 1% after 1 day, followed by an additional marginal release after 1 week.
Histological analysis revealed excellent osteoconductive properties of non-loaded Ca-P material. Inside non-loaded Ti-mesh fiber scaffolds, also bone ingrowth occurred. Quantification of the bone ingrowth showed that bone formation was increased significantly in all scaffold materials by administration of rhTGF-β
1 and rhBMP-2.
Consequently, we conclude that the release kinetics of growth factors from porous CaP cement differs from other scaffold materials, like metals and polymers. Nevertheless, orthotopic bone formation in a rabbit cranial defect model was stimulated in rhTGF-β
1- and rhBMP-2-loaded CaP cement and Ti-fiber mesh scaffolds compared with non-loaded implants.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Bone Cements</subject><subject>Bone engineering</subject><subject>Bone Morphogenetic Protein 2</subject><subject>Bone Morphogenetic Proteins - administration & dosage</subject><subject>Bone Morphogenetic Proteins - secretion</subject><subject>Calcium phosphate cement</subject><subject>Calcium Phosphates</subject><subject>Female</subject><subject>General pharmacology</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Osteogenesis - drug effects</subject><subject>Osteoinduction</subject><subject>Pharmaceutical technology. Pharmaceutical industry</subject><subject>Pharmacology. Drug treatments</subject><subject>Rabbits</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Recombinant human BMP-2</subject><subject>Recombinant Proteins - administration & dosage</subject><subject>Tissue Engineering</subject><subject>Titanium - administration & dosage</subject><subject>Titanium fiber mesh</subject><subject>Transforming Growth Factor beta - administration & dosage</subject><subject>Transforming Growth Factor beta - secretion</subject><subject>Transforming growth factor beta-1</subject><subject>Transforming Growth Factor beta1</subject><issn>0168-3659</issn><issn>1873-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0E1P4zAQgGFrxWpbyv4EUC7sLWEcxx85IYSWgoTEBc6WY4-7rtIY7JQV_55UjcSRky-PZ0YvIecUKgpUXG2rrY1Dwr6qAZoKZAXAf5AlVZKVTdvyE7KcnCqZ4O2CnOa8hUmwRv4iC8q5Uqptl4StU_w__iu8sWNMZR-NQ1dka7yPvcuFj6no4oAFDpswIKYwbM7IT2_6jL_nd0Ve7v4-396Xj0_rh9ubx9I2LYwlGlReUWDUqkY54TrJ61a62tbOd1xK3xhJkQph60aAkLVUgA0wI5TsaM1W5M9x7muKb3vMo96FbLHvzYBxn7WQjAMT30MqlWIt0AnyI7Qp5pzQ69cUdiZ9aAr6kFVv9ZxVH7JqkPoQbUUu5gX7bofu69fccQKXMzBTu94nM9iQv5xgXFB6GHR9dDh1ew-YdLYBB4suJLSjdjF8c8ondT2W3w</recordid><startdate>20050103</startdate><enddate>20050103</enddate><creator>Jansen, J.A.</creator><creator>Vehof, J.W.M.</creator><creator>Ruhé, P.Q.</creator><creator>Kroeze-Deutman, H.</creator><creator>Kuboki, Y.</creator><creator>Takita, H.</creator><creator>Hedberg, E.L.</creator><creator>Mikos, A.G.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QO</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20050103</creationdate><title>Growth factor-loaded scaffolds for bone engineering</title><author>Jansen, J.A. ; Vehof, J.W.M. ; Ruhé, P.Q. ; Kroeze-Deutman, H. ; Kuboki, Y. ; Takita, H. ; Hedberg, E.L. ; Mikos, A.G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-eae8f81031c848d6db75297d2c2dfb577f4a71e166c2460672780e403a687b123</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Bone Cements</topic><topic>Bone engineering</topic><topic>Bone Morphogenetic Protein 2</topic><topic>Bone Morphogenetic Proteins - administration & dosage</topic><topic>Bone Morphogenetic Proteins - secretion</topic><topic>Calcium phosphate cement</topic><topic>Calcium Phosphates</topic><topic>Female</topic><topic>General pharmacology</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Osteogenesis - drug effects</topic><topic>Osteoinduction</topic><topic>Pharmaceutical technology. Pharmaceutical industry</topic><topic>Pharmacology. Drug treatments</topic><topic>Rabbits</topic><topic>Rats</topic><topic>Rats, Wistar</topic><topic>Recombinant human BMP-2</topic><topic>Recombinant Proteins - administration & dosage</topic><topic>Tissue Engineering</topic><topic>Titanium - administration & dosage</topic><topic>Titanium fiber mesh</topic><topic>Transforming Growth Factor beta - administration & dosage</topic><topic>Transforming Growth Factor beta - secretion</topic><topic>Transforming growth factor beta-1</topic><topic>Transforming Growth Factor beta1</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Jansen, J.A.</creatorcontrib><creatorcontrib>Vehof, J.W.M.</creatorcontrib><creatorcontrib>Ruhé, P.Q.</creatorcontrib><creatorcontrib>Kroeze-Deutman, H.</creatorcontrib><creatorcontrib>Kuboki, Y.</creatorcontrib><creatorcontrib>Takita, H.</creatorcontrib><creatorcontrib>Hedberg, E.L.</creatorcontrib><creatorcontrib>Mikos, A.G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Biotechnology Research Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of controlled release</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Jansen, J.A.</au><au>Vehof, J.W.M.</au><au>Ruhé, P.Q.</au><au>Kroeze-Deutman, H.</au><au>Kuboki, Y.</au><au>Takita, H.</au><au>Hedberg, E.L.</au><au>Mikos, A.G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Growth factor-loaded scaffolds for bone engineering</atitle><jtitle>Journal of controlled release</jtitle><addtitle>J Control Release</addtitle><date>2005-01-03</date><risdate>2005</risdate><volume>101</volume><issue>1</issue><spage>127</spage><epage>136</epage><pages>127-136</pages><issn>0168-3659</issn><eissn>1873-4995</eissn><coden>JCREEC</coden><abstract>The objective of the study presented here was to investigate the bone inductive properties as well as release kinetics of rhTGF-β1- and rhBMP-2-loaded Ti-fiber mesh and CaP cement scaffolds. Therefore, Ti-fiber mesh and porous CaP cement scaffolds were provided with these growth factors and inserted in subcutaneous and cranial implant locations in rats and rabbits.
In vitro, a rapid release of rhTGF-β
1 was observed during the first 2 h of the Ti-fiber mesh scaffolds. During this time, more than 50% of the total dose of rhTGF-β
1 was released. Following this initial peak, a decline in the level of rhTGF-β
1 occurred. After 1 week, the entire theoretical initial dose was observed to have been released. This in contrast to the rhTGF-β
1 and rhBMP-2 release of the porous CaP cement scaffolds. Here, no substantial initial burst release was observed. The scaffolds showed an initial release of about 1% after 1 day, followed by an additional marginal release after 1 week.
Histological analysis revealed excellent osteoconductive properties of non-loaded Ca-P material. Inside non-loaded Ti-mesh fiber scaffolds, also bone ingrowth occurred. Quantification of the bone ingrowth showed that bone formation was increased significantly in all scaffold materials by administration of rhTGF-β
1 and rhBMP-2.
Consequently, we conclude that the release kinetics of growth factors from porous CaP cement differs from other scaffold materials, like metals and polymers. Nevertheless, orthotopic bone formation in a rabbit cranial defect model was stimulated in rhTGF-β
1- and rhBMP-2-loaded CaP cement and Ti-fiber mesh scaffolds compared with non-loaded implants.</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>15588899</pmid><doi>10.1016/j.jconrel.2004.07.005</doi><tpages>10</tpages></addata></record> |
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| subjects | Animals Biological and medical sciences Bone Cements Bone engineering Bone Morphogenetic Protein 2 Bone Morphogenetic Proteins - administration & dosage Bone Morphogenetic Proteins - secretion Calcium phosphate cement Calcium Phosphates Female General pharmacology Male Medical sciences Osteogenesis - drug effects Osteoinduction Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Rabbits Rats Rats, Wistar Recombinant human BMP-2 Recombinant Proteins - administration & dosage Tissue Engineering Titanium - administration & dosage Titanium fiber mesh Transforming Growth Factor beta - administration & dosage Transforming Growth Factor beta - secretion Transforming growth factor beta-1 Transforming Growth Factor beta1 |
| title | Growth factor-loaded scaffolds for bone engineering |
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